SANTRAUKA Darbo, kurio tema „Nevaisingų moterų folikulinio skysčio lątelinės sudėties ir interferono- γ koncentracijos tyrimas“, tikslas buvo nustatyti ir įvertinti leukocitų populiacijas ir interferono- γ koncentraciją nevaisingų moterų folikuliniame skystyje. Šiam tikslui pasiekti buvo iškelti uždaviniai: nustatyti ir įvertinti nevaisingų moterų folikulinio skysčio leukocitų kiekį; įvertinti nevaisingų moterų folikulinio skysčio leukocitų populiacijas; nustatyti ir įvertinti interferono- γ koncentraciją folikuliniame skystyje. Tyrimų objektas –folikuliniame skystyje esančios ląstelės ir jų išskiriami citokinai.Tiriamos 25 – 37 metų amžiaus moterys, kurioms planuojamas atlikti dirbtinis apvaisinimas. Tiriamoje medžiagoje skaičiuotas bendras leukocitų skaičius ( šviesiniu mikroskopu ), naudojant Goriajevo kamerą. Leukocitų populiacijos išaiškintos apžiūrint preparatus pro mikroskopą, naudojant šviesinį mikroskopą, imersinę sistemą. Interferono- γ koncentracija nustatyta imunofermentiniu metodu. Atlikus visus tyrimus, jie buvo statistiškai apdoroti.Tirtas septyniasdešimt vienos moters folikulinis skystis. Tiriamosios sugrupuotos pagal ovuliacijos pobūdį ir nevaisingumą sukėlusią priežastį, taip pat buvo dalis moterų, kurioms nevaisingumas nebuvo diagnozuotas, nevaisingi buvo jų sutuoktiniai.Tiriant nevaisingų moterų folikulinį skystį, absoliutus leukocitų skaičius buvo: esant normaliai ovuliacijai 0,58+0,30x106 ,esant nenormaliai ovuliacijai 0,65+ 0,26x106... [toliau žr. visą tekstą] / SUMMARY The investigation of the cell composition of the folicular liquid and the concentration of the interferon- γ in infertile women The aim of this study was to determine and evaluate populations of leukocytes and the concentration of interferon-γ in folicular liquid in infertile women. The tasks were set to reach the objective as follows: to determine and evaluate leukocyte count in folicular liquid in infertile women; to evaluate populations of leukocytes in folicular liquid in infertile women; to determine and evaluate concentration of interferon- γ in folicular liquid. Cells, present in the folicular liquid and cytokines they exude, are the object of the research. Women, from 25 to 37 years and who are planing an artificial impregnation procedure, take part in this study. Total leukocyte count of the best sample was measured using light microscope and Goriaev chamber. Populations of leukocytes were analysed using light microscope and immersive system. Concentration of interferon- γ was determined using the immunoenzyme method. After the investigations were conducted, the data was statistically processed. The folicular liquid of 71 women was investigated. The studied women were divided into groups by character of ovulation and reason inducing their infertility; and there was also a group of women to whom the infertifily was not diagnosed, their spouses were infertile. When investigating the folicular liquid of infertile women, the absolute leukocyte... [to full text]

The objective of the study is to investigate the total antioxidative capacity of plasma and the level of survival of non-activated neutrophilic leucocytes in peripheral venous blood, their oxidative function and its changes when activated by non-opsonised E.coli and S.aureus, exposed to glucose and investigate the effect of glycosylated haemoglobin (HbA1c%) of different concentrations, using the methods of luminol- and liucigenin-dependent chemiluminescence, with respect to the patients suffering from type 1 diabetes mellitus (DM) and a severe form of periodontitis, as well as those who suffer from type 1 diabetes mellitus, but have healthy periodontal tissues, and also persons without systemic diseases and inflammatory periodontal diseases. An integrated investigation of the level of oxidative capacity of neutrophilic leucocytes in peripheral venous blood, using the methods of luminol- and liucigenin-dependent chemiluminescence, of the patients who suffer from type 1 diabetes mellitus and a severe form of periodontitis as well as persons suffering from type 1 diabetes mellitus, but with no inflammatory periodontal disease, and also healthy persons, has been performed for the first time. A new presumption has been presented on the basis of the collected material, which states that the development of inflammatory diseases of periodontal tissues is determined by the decreased level of survival of bactericidal myeloperoxidase system of neutrophilic leucocytes.