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Syntheses, reactivities and biological activities of ruthenium azido, nitrido and nitrosyl complexes supported by tetradentate tertiaryamine ligandsLeung, Hiu-chi., 梁曉詞. January 2010 (has links)
published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy
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Development of lymphocyte specific internalising aptamersMillroy, Laura Ann 23 April 2014 (has links)
Aptamers are synthetic nucleic acid molecules designed to bind with high specificity and affinity to a selected target. The aptamer selection method, called the systematic evolution of ligands by exponential enrichment (SELEX), was first described in 1990 and has been adapted for the selection of aptamers for a number of applications. One such application is the selective targeting of cells for therapeutic delivery. This thesis explores this application with the selection and characterisation of internalising aptamers specific to the T lymphocyte specific receptor, CD7. The CD7 receptor is expressed on thymus derived progenitor lymphocytes and remains after T cell activation and expression of the CD4 receptor. As such, the CD7 receptor is a noteworthy target for lymphocyte cancers, HIV-1 and other T lymphocyte tropic viruses.
A heterogeneous pool of internalising CD7-aptamers was enriched through six rounds of positive selection in a stably transduced CD7-HeLa cell line. Aptamers were selected using a modified whole cell SELEX method that selected specifically for internalising aptamers. Aptamer specificity for CD7-HeLa cells over HeLa cells was screened by flow cytometry. CD7 specific aptamers were screened for binding after blocking CD7-HeLa cells with an anti-CD7 antibody. Eight CD7 specific aptamer clones were selected from CD7-HeLa screening for evaluation in Jurkat cells (T lymphocyte cell line endogenously expressing the CD7 receptor). Three aptamer clones showed high level binding to Jurkat cells by flow cytometry (CSIR 3.14, CSIR 3.37 and CSIR 3.42). Kinetic analysis of aptamer internalisation was analysed using flow cytometry and determined to be within the femtomolar range. Aptamer CSIR 3.14 had a dissociation constant of 2.1 fM and an association rate of 4.7 ± 2.4 × 105 Molar-1minute-1, aptamer CSIR 3.37 had a dissociation constant of 0.23 fM and an association rate of 4.3 ± 3.3 × 106 Molar-1minute-1 and aptamer CSIR 3.42 had a dissociation constant of 1.1 fM with an association rate of 7.9 ± 5.1 × 105 Molar-1minute-1. Aptamer CSIR 3.14 internalisation was tracked by confocal microscopy and the kinetics calculated with an association rate of 6.3 × 104 Molar-1minute-1 and Kd of 13 fM. Deletions within the CSIR 3.14 sequence that altered the predicted structures significantly reduced the aptamer binding. Combined, the data presented in this thesis identifies aptamer CSIR 3.14 as a lymphocyte specific internalising aptamer with potential for therapeutic delivery.
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Neuron-ligand pathfinding on surfaces modified by laminin and laminin-derived peptidesLeng, Ying. January 2006 (has links)
Thesis (M.S.)--University of Delaware, 2006. / Principal faculty advisor: Thomas P. Beebe, Jr., Dept. of Chemistry & Biochemistry. Includes bibliographical references.
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Syntheses, reactivities and biological activities of ruthenium azido, nitrido and nitrosyl complexes supported by tetradentate tertiary amine ligandsLeung, Hiu-chi. January 2010 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2010. / Includes bibliographical references (leaves 196-198). Also available in print.
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Synthesis and characterization of group 4 complexes and olefin polymerization catalysts supported by chelating v-aryl ligands /Lo, Chun Yu. January 2009 (has links) (PDF)
Thesis (M.Phil.)--City University of Hong Kong, 2009. / "Submitted to Department of Biology and Chemistry in partial fulfillment of the requirements for the degree of Master of Philosophy." Includes bibliographical references.
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Metal complexes containing oxygen tripod ligands : models of metal oxides /Lam, Chong Ho. January 2003 (has links)
Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2003. / Includes bibliographical references (leaves 275-290). Also available in electronic version. Access restricted to campus users.
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Cell biology on NKG2D ligands and NK cell recognitionAgüera-González, Sonia January 2011 (has links)
No description available.
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Characterisation of protein-ligand interactions and their application to drug discoverySchreyer, Adrian Michael January 2011 (has links)
No description available.
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The synthesis and characterization by use of spectroscopic and x- ray methods of bromo, phosphine, and nitro derivatives of 13-phenyl- 1,4,7,10-tetraoxa-13-azacyclopentadecaneSheu, Biing-Jahn January 1992 (has links)
The phenyl ring in the crown ether, 13-phenyl-1,4,7,10-tetraoxa-13-azacyclopentadecane (I), was used a site for functionalizing the compound. Electrophilic bromination of the ring with tribromide ion gave a 95% yield of the product substituted in the para position. This product underwent lithium-bromine exchange when reacted with n-butyllithium. The resulting anion was used to prepare PhZPX and PhPX2 derivatives, X p-C6H4NCH2(CH2OCH2)4&12 The oxide of PhZPX was completely characterized by an x-ray diffraction study which showed, in general, that the phosphorus was tetrahedral, the nitrogen planar, and the crown ether ring organized with the oxygen atoms endodentate. Several attemps were made to nitrate or nitrosate the phenyl ring in the parent crown ether. Spectroscopic evidence obtained from the products indicate that the reaction led to mixtures of mono and disubstituted products. / Department of Chemistry
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Application of cobalt complexes containing SNS ligands as catalysts for biomimetic paraffin activation.Komarsamy, Lynette. 23 April 2014 (has links)
A series of SNS ligands have been successfully synthesised and characterised by IR, NMR and
MS. The ligands are divided into two groups and represented by the general formulae: 2,6-
bis(RSCH2)pyridine [R= methyl, ethyl, butyl, cyclohexyl, phenyl] and bis(RSCH2CH2)amine
[R= ethyl, butyl, decyl]. Cobalt complexes of the respective ligands with the general formulae
Co[2,6-bis(RSCH2)pyridine]Cl2 and Co[bis(RSCH2CH2)amine]Cl2 were synthesised and
characterised by IR, elemental analysis and X-ray crystallography (for selected complexes).
Thus, to investigate the electronic and steric effects of the ligand structure on the chemistry and
reactivity of the complexes, the substituents bonded to the two sulfur donor atoms were
sequentially varied and two different nitrogen sources were chosen. Crystal structures of Co[2,6-
bis(CH2SCH2)pyridine]Cl2 (Ia), Co[2,6-bis(CH2CH2SCH2) pyridine]Cl2 (IIa) and Co[2,6-
bis(CH2CH2CH2CH2SCH2)pyridine]Cl2 (IIIa) were obtained. It was found that complex Ia
exists as a molecular dimer linked through two chloride bridges resulting in an octahedral
geometry around each metal centre, while complexes IIa and IIIa are monomers exhibiting a
trigonal bypyrimidal geometry. The complexes were tested as catalysts for the activation of
paraffinic C−H bonds towards the formation of oxygenated products: octanol, octanone, octanal
and octanoic acid from the substrate n-octane. Gas chromatography was utilised to quantify the
products formed and also to calculate the conversion and selectivity of each catalyst system. The
catalytic testing revealed that the ketone products were the most dominant with selectivities of
ca. 90%. The catalyst that was the most active was Co[bis(CH2CH2SCH2CH2)amine]Cl2 (Ib)
with a total n-octane conversion of 23%. / Thesis (M.Sc.)-University of KwaZulu-Natal, Durban, 2012.
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