Submitted in partial compliance with the requirements of the Master’s Degree in Technology: Homoeopathy, Department of Homeopathy, Durban University of Technology, Durban, South Africa, 2015. / INTRODUCTION A homoeopathic drug proving was conducted by Master’s students at the Durban University of Technology (DUT). The proving substance was nail shavings obtained from an African lioness (Panthera leo). The primary objective of this study was to identify the symptoms produced by healthy individuals in response to Panthera leo 30CH; this was done by administering the proving remedy (Panthera leo 30CH) to healthy individuals, who recorded the symptoms they experienced after taking the remedy. The secondary objective was to compare the symptoms produced to the ethology of the African lion. The final objective was to further develop the materia medica by adding the remedy picture of Panthera leo. METHODOLOGY This study was qualitative and exploratory in nature. The study took the form of a randomised, double-blind and placebo controlled trial. A total of 30 provers, who met the inclusion criteria (Appendix C), participated in this study. The provers were randomly assigned to either a verum or placebo group, such that 24 received verum (Panthera leo) and 6 received placebo. Neither provers nor researchers had knowledge of which provers were in each group until the study was complete, and provers did not have any knowledge regarding the nature of the proving substance. Before commencing the study all provers signed relevant information and consent documents. The researcher then took a full case history and performed a physical examination. Provers recorded their baseline state for 7 days in a journal, prior to taking the verum or placebo, as administered to them. After recording their baseline state for 7 days, they took their remedies and continued to record their symptoms for a minimum of 2 weeks, or until the proving symptoms ceased. Once they had completed recording in their journals, a post-proving case history was taken and a physical examination was performed. All journals were collected and analysed by the researcher. The remedy was manufactured by triturating the crude substance up to the 3rd centesimal potency. This was then converted to a liquid potency and further diluted up to the 30th centesimal potency. Neutral granules were impregnated with Panthera leo 30CH, and dispensed as 10 granules in 500mg of lactose powder. The placebo was dispensed as 10 granules, which were impregnated with 96% ethanol, in 500mg lactose powder. Each prover received 6 lactose powders, containing either verum or placebo. The manufacture of Panthera leo was done according to the methods and guidelines stipulated in the German Homoeopathic Pharmacopoeia (Benyunes, 2005) RESULTS The proving of Panthera leo 30CH produced a total of 1234 rubrics, which represent a wide variety of mental, emotional and physical symptoms. The main themes that emerged from the mental and emotional symptoms included anger, aggression, irritability, poor concentration and fear. Physical symptoms included catarrh, constipation, diarrhoea, diminished appetite, fatigue, insomnia, thirstiness and headaches. A comparison was made between the symptomatology of Panthera leo and the ethology of the lion. Some of the themes of symptoms which corresponded with the ethology of the lion included ‘anger’, ‘audacity’, ‘ennui’, ‘increased appetite and thirst’, ‘responsibilities’ and ‘children’. CONCLUSION The objectives of this study were fulfilled, as the proving of Panthera leo produced a wide variety of clearly identifiable and observable symptoms amongst the healthy individuals recruited. There was a remarkable correlation of a portion of the materia medica with the ethological features of the African lion. As a result of this study, the materia medica of Panthera leo was well outlined and will be added to the existing body of homoeopathic materia medica for application and utilization in clinical practice

Thesis (MTech. degree in Game Range Management.)--Tshwane University of Technology, 2010. / Lion (Panthera leo) social organisation in the Northern Tuli Game Reserve, Botswana, and the Venetia Limpopo Nature Reserve, South Africa, was investigated. Situated 20 km apart, the Northern Tuli Game Reserve is predominantly unfenced with unrestricted animal movements, as opposed to the Venetia Limpopo Nature Reserve which is completely fenced. Historically both lion populations have faced various but similar pressures such as safari hunting, snaring, poisoning and killing due to perceived conflict with livestock farmers.

Lions have recently been introduced to a number of small (<300km²), enclosed reserves in the Eastern Cape Province as a draw-card for tourists. However, there have been few studies of lion behaviour or predator prey interactions within small reserves. Thus the aim of this study is to contribute towards a better understanding of the effects of space on diet and habitat use of re-introduced lions. The research was conducted at two small private reserves, Shamwari and Kwandwe. Kills were mostly large (55%, >120kg), adult (41%) and male (39%) and the five most commonly killed species (kudu, black wildebeest, warthog, bushbuck and Blesbuck) comprised 67.9% of all kills and 67.8% of total biomass consumed. In an attempt to overcome the problem of gaps in the kill lists, a subset of data, which excluded kills more than five days apart, was analyzed and this gave similar results to analysis of the full kill lists. Mean daily intake rates varied annually between 2.26 and 5.23kg.FEQ⁻¹ for the full data set and 4.53 and 1 0.46kg.FEQ⁻¹ for the subset. The difference between estimated (calculated using daily intake rates of 5 and 7.12kg.FEQ⁻¹) and observed offtake for each ungulate species reflected the preference ratings for that species. Analysis of bone marrow indicated that: lions killed ungulates in moderate to good condition; condition of culled animals was lower than that of kills; distal bones could be used in analyses; and that that bones exposed to the environment for at least a week could be used to analyze condition of prey. Home ranges and activity centers ranged from 21.42km² to 73.27km² and 2.7lkm² to 17.47km² respectively, which was smaller than expected from previous studies. Although, lions at K wandwe and Shan1wari preferred different vegetation types, at both reserves they generally occupied areas that were structurally similar to what has previously been reported. Continuous monitoring and analyses of diet, including condition of the prey, and habitat use will provide reserve managers with some of the necessary information to enable them to effectively manage their lion populations and to predict and minimize potential problems.

Populations of small prey species (< 92 kg) can be regulated by competition, habitat-mediated responses, climate change, disease and predation. Populations of small prey species have declined in the Addo Elephant National Park Main Camp Section since the reintroduction of apex predators (lion and spotted hyaena). One possible explanation is predation by lion and spotted hyaena. However, small prey species are not generally considered preferred prey items of these predators, hence their contribution to predator diets needs to be explored. Global Positioning System (GPS) cluster analysis has emerged as a viable approach to locate lion kills for lion diet estimation in AENP, however this technique is known to bias diet estimates to large prey species. Understanding the impact of lion predation on populations of small prey in Main Camp therefore requires better estimates of lion diets. This study investigated the potential underestimation of small prey species in lion diet estimates by combining the data from scat analysis and carcasses located at GPS clusters. The adjusted lion diets were then used to investigate if the impact of lion predation could explain declines in small prey, by relating lion prey preferences to prey population trends in Nyathi. Using the results from Nyathi, it could then be inferred whether lions could have been responsible for the declines observed in Main Camp. Six GPS-collared lions provided location data used to construct individual movement paths, along which spatio-temporal clusters of GPS locations (n = 4896) were identified for ground investigation. Carcasses (n = 643) were recorded at the GPS clusters and scats (n = 256) were collected at GPS clusters with or without carcasses. Data from the located carcasses and scat collections were then used to create a history of feeding events for each collared lion in Main Camp-Colchester and Nyathi from a) carcasses alone or b) a combination of scats and carcasses, based on an estimated short and long lion throughout period. Relative prey availability was estimated based on camera trapping in Nyathi in high lion, low lion and no lion use areas, as lions may encounter some prey species more than other prey species. Jacobs’ index prey preference was used to investigate the importance of prey species to lion diets at three landscape scales: 1) Nyathi, 2) lion UD and 3) core lion UD. Lastly, prey population trends were investigated in Nyathi to provide an indication of possible changes in prey populations. Large prey species dominated the adjusted diets of lions in Main Camp-Colchester and Nyathi. However, when comparing the GPS carcass-derived lion diet estimate to the combined lion diet estimate, small prey were underestimated more often than large prey in the GPS carcass-derived lion diet estimate. Missed feeding events were therefore a function of prey body size. In addition, missed feeding events were also representative of the lion diets based on carcasses, with the tendency for species more often consumed to be missed more often in the diet record. However, even though small prey species were underestimated, they represented the minority of species consumed by lions and were predominantly avoided by lions in Nyathi. Lion prey preferences thus did not correlate to the declines in small prey species in Naythi, suggesting other potential drivers are important for small population trends in Nyathi. Lion predation cannot be ruled out as a driver of the decline in small prey species in Main Camp, due to different ecological conditions (e.g. vegetation density) in Main Camp compared to Nyathi, during the observed small prey population declines. Lion prey preferences in Nyathi revealed fine-scale variations in species specific preferences by lions, as well as site-specific differences in prey preferences when compared to published lion prey preference meta-analyses. These findings suggest that metaanalysis studies should be used as a guideline from which hypotheses about local predatorprey interactions can be developed rather than as an absolute measure of prey preference.

The reintroduction of lions, cheetahs and African wild dogs to Kwandwe Private Game Reserve in the Eastern Cape Province of South Africa created the opportunity to study the biology and behaviour of these large carnivores in an enclosed system. The research focused on space utilisation and feeding ecology of the predators, using standard observational methods. Changes in the structure of the carnivore guild, and changes in the prey base that occurred during the study were used as natural experiments to examine factors affecting space use and diet. In general, the diets of the predators were similar to previous studies and prey abundance was more important than prey species size in determining prey selection. Changes in the abundance of certain prey species was matched by a dietary switch in lions from kudu to warthog and an increase in the proportion of springbok kills by the cheetahs. There was no significant difference in the proportions of prey species detected by ad hoc or continuous observations. Core areas occupied by the predators were significantly smaller than home ranges except when females were denning. Home ranges overlapped both within and between species, but there was very little overlap of core areas. An increase in the number of lion prides in the area during the study resulted in an increase in overlap of home ranges of lions and cheetahs, but did not result in a change in home range size. Space use by female cheetahs with cubs increased as the cubs grew older. The use of thicket vegetation by cheetahs decreased with an increase in the number of lion prides. Minimum Daily Energy Expenditure, energy intake and net benefit were calculated for the predators using data from continuous observations. All predators exhibited a large net benefit and the net benefit for single female cheetahs was greater than for the members of the coalition. Net benefit for the alpha pair of African wild dogs was lower than that of the pack.

The re-introduction of large carnivores into relatively small conservation areas that fall within the historic distribution range of the species is becoming an increasingly common occurrence. The success of such re-introductions depends very much on the quality of the information that is available to guide management decisions, but in many cases, little information is available. The re-introduction of lions and cheetahs to Sanbona created the opportunity to monitor the behaviour of re-introduced predators to a relatively large system that was characterised by a low ungulate stocking density and little standing water. The broad aims were to study the feeding and spatial ecologies of the lions and cheetahs, to collect standard base-line data, and to examine the effects of the low prey density and limited standing water on habitat selection, range size and diet. The diet (data collected from direct observation and faecal analysis) was similar to that reported in previous studies, and lions and cheetahs preferred greater kudu, black wildebeest and springbok. Lions preferred medium to large prey items, and cheetahs preferred medium to small prey items. The hilly and mountainous terrain of much of the reserve meant that only 50% of the total space was available to the predators. Home ranges of most of the predators were focused around the single large body of standing water. This is likely to have been a response to the water, the vegetation, and the prey that was attracted to these. Habitat selection was also influenced by inter and intra-specific interactions at least for a solitary male lion and female cheetahs. Range sizes were larger than on some other reserves and it is suggested that this was a result of the low prey density. These results form the basis for management recommendations including the importance of continuing to monitor the system and opening up additional parts of the reserve to the predators.

Shamwari Game Reserve was the first enclosed conservation area in the Eastern Cape Province of South Africa to reintroduce free ranging lions, cheetahs, leopards and wild dogs back into their historic range. At that time (2000 – 2001), little information was available on the ecology and behaviour of these predators in the habitats of the Eastern Cape, and management decisions were based on assumptions and unfounded comparisons with extant populations but from quite different habitats. The aim of this study was therefore to obtain a better understanding of the feeding ecology and space use of the predator guild, and the carrying capacity of Shamwari Game Reserve to enable more informed management decisions. In addition, the reserve is a photographic based tourism venture and understanding both the ecological and financial sustainability of the predator guild was important. The diets of the predators were similar to those reported in other studies; larger predators killed a greater range of prey species than did smaller predators and a small number of prey species made up the majority of the kills. The larger predators had a higher mean kill mass than the smaller species and prey selection was influenced by prey size, prey abundance and prey habitat preference, and risk associated with hunting the species. Diet was flexible and responded to natural and management induced changes in prey abundance. There was a considerable overlap in space use by the lions, cheetahs and leopards with their core areas being centred on and around the Bushmans River. Space use was driven by resource distribution and landscape attributes, and by the presence of other predators of the same or different species. The long term viability of wild dog within the reserve was explored and the results confirmed that there was neither the required space nor the ecological processes and the wild dogs were removed from the reserve. A carrying capacity of the reserve for the predator guild was determined using the Maximum Sustainable Yield method to assess the potential prey species off take and a resultant density of 3.3 to 6.6 lion female equivalent units per 10 000 ha was established. The natural carrying capacity of the reserve with respect to predators will not sustain the tourism objectives and consequently prey supplementation was necessary to maintain predator density at levels high enough to sustain tourism. Under these conditions the large predator guild is still sustainable financially although careful, responsible management is needed to provide ecological sustainability.