Thesis (M.A. (Dept. of Medical BioScience, Faculty of Natural Sciences))--University of the Western Cape, 2009. / Includes bibliographical references.

Thesis (M.Sc.)--College of William and Mary. / Vita. Includes bibliographical references.

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by Chi-Ming Wong. / Bibliography: leaves 183-193 / Thesis (M.Ph.)--Chinese University of Hong Kong, 1984

Thesis (M.Sc.)--Memorial University of Newfoundland, 2001. / Restricted until June 2002. Bibliography: leaves 126-136.

Thesis (M. Sc.)--Memorial University of Newfoundland, 1997. / Restricted until November 1998. Bibliography: leaves 94-106.

Obesity and type II diabetes are associated with dementia and Alzheimer's disease. A high-fat diet induces memory deficits in rodents, however, complex episodic-like memory, has not been tested. Episodic memory is the recollection of events using a “what-where-when/which” experience and is the first memory to be compromised in Alzheimer's disease. To identify a link between a high-fat diet and episodic memory, 12 week old, male, C57Bl/6 mice, were fed a semi purified high-fat or low-fat diet ad libitum and tested with object-place-context (episodic-like), novel object recognition, object-place (spatial) and object-context (contextual) memory tasks for up to 2 weeks. A separate group of animals were fed a high-fat diet for 1 week followed by a low-fat diet for 1 week. Animals were killed after 3 days, 1 week or 2 weeks on diet. Brains were kept frozen until the hippocampus was dissected and proteomics performed. Further studies were carried out in rat primary hippocampal cell cultures to investigate the impact of different fatty acids on neuronal dendritic morphology. We found that episodic-like memory is compromised after only one day of a high fat diet together with spatial and contextual tasks. The ability to carry out the novel object recognition test remained intact. Proteomic analysis of hippocampal tissue revealed changes in a number of proteins associated with metabolism, cell stress, cell signalling, inflammation and the cytoskeleton. High-fat diet induced changes were reversed by a low-fat diet. Hippocampal neuron cultures showed that long chain saturated fatty acid palmitic acid, a component of the high-fat diet used in the behavioural and proteomic studies, caused reduced dendritic arborisation whist n-3 polyunsaturated fat docosahexaenoic acid negated these effects. These data link high-fat diet to indices of hippocampal neuronal damage and memory deficits and have implications for the link between diet, obesity and cognitive decline.

Twenty-three university male students: were fed mixed food diets, containing either 400 mg or 1400 mg cholesterol/day under controlled dietary conditions for 4 consecutive weeks. One group received a diet containing 400 mg cholesterol/day, which was contributed by non-egg food sources. The other group received a similar diet with the daily addition of 1000 mg cholesterol as 4 whole eggs. Diets were adjusted so differences in total protein, carbohydrate, fat, and fatty acid composition were minimal between the two dietary treatments. Plasma total cholesterol, triglycerides, and HDL, LDL, and VLDL cholesterol levels were measured at the beginning, weekly throughout the experimental period, and 1 week after completion of the study. No significant changes in any of the measured parameters were observed as a result of the high cholesterol diet. Comparisons of BDL, LDL, and VLDL cholesterol concentrations expressed as percentages of plasma total cholesterol showed that lipoprotein cholesterol distribution remained unchanged within and between treatments throughout the study. Relative concentrations of plasma lipoproteins at the beginning or the end of dietary treatment were similar in subjects receiving 400 mg or 1400 mg cholesterol/day. No detectable ApoE was observed by SDS-PAGE in HDL₂ or HDL₃ samples from either treatment group. / Ph. D.

Thesis (M.Sc.)--Memorial University of Newfoundland, 1999. / Restricted until November 2000. Bibliography: leaves 117-126.