Schistosomiasis is one of the most widespread parasite diseases in the world, whereas Schistosoma mansoni is a major schistosome species in Africa, America, and the Caribbean islets. Many antigenic vaccine candidates have been postulated, including sm22.6 and GST. Although the lower level of re-infection of human schistosomiasis is related to the higher level of IgE against rsm22.6, unfortunately, the observation of the experimental vaccination in mice finds some difficulties in further development of vaccine. In addition, the biochemical and biophysical properties of the antigen are virtually unknown, thus the present study intends to characterize sm22.6 from biochemistry and cell biology. To do this, sm22.6 was expressed in E. coli (BL21DE3) and purified to homogeneity. Analyses of the recombinant protein showed that the antigen was highly hydrophobic and formed polymers readily as judged by both native and denatured electrophoreses. Because various technologies including NMR and DNA binding which had been applied to the study of the antigen generated vague results, we decided to express the antigen in human breast cancer cell (MDA-MB-435s) to locate in the subcellular compartments where the antigen is situated. Results showed that the antigen, not like the recombinant expressed in E. coli, located in both cellular fluids and membrane, suggesting that the antigen might not be a skeleton protein as predicted by proteomics.

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Mobile robots are used for a wide range of purposes such as mapping an environment and transporting material goods. Regardless of the specific application, the navigation of the mobile robot is usually divided into three separate parts: localization, path planning and path execution. Localization is the process of determining the location of the robot with respect to a reference coordinate system. There are many different approaches to localizing a mobile robot which employ a wide variety of sensors. The objective of my research is to develop a method for the localization of multiple mobile robots equipped with inexpensive range sensors in an indoor environment. Each mobile robot will be equipped with a rotating infrared sensor and a rotating CMOS camera. The multiple mobile robot system will be treated as a linked robot for localization. The proposed localization method is verified via both simulation and experiment. Through the use of the virtual link length and relative heading information, a system of mobile robots can be effectively localized using detected environmental features.

Software testing is the most commonly used methodology for validating quality of software systems. Conceptually, testing is simple, but in practice, given the huge (practically infinite) space of inputs to test against, it requires solving a number of challenging problems, including evaluating and reusing tests efficiently and effectively as software evolves. While software testing research has seen much progress in recent years, many crucial bugs still evade state-of-the-art approaches and cause significant monetary losses and sometimes are responsible for loss of life. My thesis is that a unified, bi-dimensional, change-driven methodology can form the basis of novel techniques and tools that can make testing significantly more effective and efficient, and allow us to find more bugs at a reduced cost. We propose a novel unification of the following two dimensions of change: (1) real manual changes made by programmers, e.g., as commonly used to support more effective and efficient regression testing techniques; and (2) mechanically introduced changes to code or specifications, e.g., as originally conceived in mutation testing for evaluating quality of test suites. We believe such unification can lay the foundation of a scalable and highly effective methodology for testing and maintaining real software systems. The primary contribution of my thesis is two-fold. One, it introduces new techniques to address central problems in both regression testing (e.g., test prioritization) and mutation testing (e.g., selective mutation testing). Two, it introduces a new methodology that uses the foundations of regression testing to speed up mutation testing, and also uses the foundations of mutation testing to help with the fault localization problem raised in regression testing. The central ideas are embodied in a suite of prototype tools. Rigorous experimental evaluation is used to validate the efficacy of the proposed techniques using a variety of real-world Java programs. / text

ITC/USA 2013 Conference Proceedings / The Forty-Ninth Annual International Telemetering Conference and Technical Exhibition / October 21-24, 2013 / Bally's Hotel & Convention Center, Las Vegas, NV / Telemetry ground stations spread over geographically diverse areas are well suited for use in passively locating the source of a distant transmitted signal. In a favorable positioning of receive sites, the accuracy of these passive localization techniques can compete with the accuracy of radars. In these cases, use of receive only assets is a less expensive alternative than the use of a radar's scarce resources. Until recently, the major technical challenge to implementation of the passive localization techniques of time-difference of arrival (TDOA) and frequency-difference of arrival (FDOA) has been the frequency and time stability of geographically separated receivers. Advances in GPS based timing and frequency references has made the implementation of TDOA and FDOA feasible. This paper shows how these limitations have been overcome using the current telemetry assets at the Reagan Test Site in Kwajalein Atoll.

The scientific interface between atomic, molecular and optical (AMO) physics, condensed matter, and quantum information science has recently led to the development of new insights and tools that bridge the gap between macroscopic quantum behavior and detailed microscopic intuition. While the dialogue between these fields has sharpened our understanding of quantum theory, it has also raised a bevy of new questions regarding the out-of-equilibrium dynamics and control of many-body systems. This thesis is motivated by experimental advances that make it possible to produce and probe isolated, strongly interacting ensembles of disordered particles, as found in systems ranging from trapped ions and Rydberg atoms to ultracold polar molecules and spin defects in the solid state. The presence of strong interactions in these systems underlies their potential for exploring correlated many-body physics and this thesis presents recent results on realizing fractionalization and localization. From a complementary perspective, the controlled manipulation of individual quanta can also enable the bottom-up construction of quantum devices. To this end, this thesis also describes blueprints for a room-temperature quantum computer, quantum credit cards and nanoscale quantum thermometry. / Physics

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Recent behavioral studies of damage to the hippocampus have demonstrated such effects as: (a) inability to inhibit a previously learned response (Clark & Isaacson, 1965: Ellen & Wilson, 1963: Isaacson & Wicklegren, 19631 Kimble, 1963: Kimble, Kirby, & Stein, in press: Kimura, 1958), (b) perseveration of a learned response (Gross, Chorover & Cohen, 1965: Teitelbaum, 1964: Webster & Voneida, 1964: Wicklegren & Isaacson, 1963), (c) increased activity and reduced spontaneous alternation (Douglas & Isaacson, 1964: Kimble, 1963: Teitelbaum & Milner, 1963), (d) consolidation deficits (Madsen & Kimble, in press), (e) recent memory deficits (Drachman & Ommaya, 1964). [...]