Magister Scientiae Dentium - MSc(Dent) / Human identification is one of the major responsibilities in the field of Forensic Odontology as it plays a role in identifying deceased individuals using their oral and dental structures. Furthermore, human identification could be a challenging procedure in catastrophic disasters and mass fatality incidents in terms of decomposed and skeletonized human remains. Identification process can be applied using different methods such as fingerprint, DNA and detention. Gender-determination of the victim facilitates reconstruction and rebuilding in the profiling of a medico-legal case investigation. Gender-determination facilitates the procedure up to 50% for positive identification. Anthropology studies have shown that the sexual dimorphism of skeletal remains and teeth can facilitate the identification process. However, teeth are the hardest structures in the human body and virtually immortal as they can withstand diverse circumstances This study aimed to investigate sexual dimorphism of teeth by measuring the mesiodistal width of the maxillary and mandibular permanent canines in a sample of the South African population living in Cape Town. Two hundred orthodontic study models were used, 50 males and 50 females, between 13-30 years of age.

Cerebral blood flow (CBF) measures are central to the investigation of ischemic strokes, spreading depressions, functional and neuronal activation. Laser Speckle Contrast Imaging (LSCI) is an optical imaging technique that has been used to obtain CBF measures in vivo at high spatial and temporal resolutions, by quantifying the localized spatial blurring of backscattered coherent light induced by blood flow. Despite being widely used for biomedical applications, LSCI's critical limitations such as its tendency to underestimate large flow changes and its inability to accurately estimate CBF through a thinned skull have not been overcome. This dissertation presents a new Multi Exposure Speckle Imaging (MESI) technique that combines a new instrument and mathematical model to overcome these limitations. Additionally, in a pilot clinical study, an adapted neurosurgical microscope was used to obtain intra-operative LSCI images of CBF in humans. The MESI instrument accurately estimates experimental constants by imaging backscattered speckles over a wide range of the camera's exposure durations. The MESI mathematical model helps account for light that has scattered from both static and moving particles. In controlled flow experiments using tissue simulating phantoms, the MESI technique was found to estimate large changes in flow accurately and the estimates of flow changes were found to be unaffected by the presence of static particles in these phantoms. In an in vivo experiment in which the middle cerebral artery in mice was occluded to induce ~100% reduction in CBF, not only was the reduction in CBF accurately estimated by the MESI technique but these estimates of CBF changes were found to be unaffected by the presence of a thinned skull. The validity of statistical models used to derive the MESI mathematical model was confirmed using in vivo dynamic light scattering (DLS) measurements of CBF in mice. The MESI technique's potential to estimate absolute values of CBF in vivo was demonstrated by comparing CBF estimates obtained using the MESI technique to DLS measurements. The MESI technique's ability to measure CBF changes quantitatively through a thinned skull makes it particularly useful in chronic and long term studies leading to the development of better, more accurate stroke models. / text