The skin conductance response is involved in the preparation for and response to stimuli with emotional significance. The neural mechanisms responsible for the generation of the skin conductance response are not well understood despite the common use of this signal as an index of emotional response. Data from anatomical, lesion, and neuroimaging studies in humans suggest that the amygdala, a component of the brain circuit for emotion, plays a critical role in the generation of the skin conductance response. Here we employ a novel combination of existing techniques to understand the stimuli that elicit skin conductance responses in the monkey and the neural mechanisms in the amygdala that participate in its generation. We recorded skin conductance responses in monkeys trained to perform a passive image viewing task. This paradigm is a staple of human emotion research but to date has not been adapted to the monkey. In addition, skin conductance responses to these stimuli were recorded in conjunction with single unit responses from the amygdala. This study addresses the relationship between the activity of single neurons recorded from identified nuclei of the monkey amygdala and autonomic responses. Neurons in multiple nuclei of the amygdala showed reliable changes in neuronal discharge prior to the skin conductance response. These neurons were primarily in the dorsal nuclei of the amygdala, which confirms predictions made from anatomical and neuroimaging data. It is suggested that these changes in neuronal discharge may correspond to the generation of this autonomic component of the expression of emotion.

Solo percussionist and composer Glenn Kotche has achieved international fame with his experimental percussion-based music, collaborating with and composing for renowned contemporary music ensembles such as the Kronos Quartet, Band on a Can All-Stars, So Percussion, and Eighth Blackbird. One of his most celebrated compositions in recent years is Monkey Chant, which combines acoustic and electronic elements in a solo multiple percussion setting. Written and premiered in 2006, Kotche was inspired to compose Monkey Chant after listening to original field recordings in Bali from the Nonesuch Explorer Series. Found in these recordings is the popular Balinese music and dance drama known as Kecak. Monkey Chant showcases, through percussion, the intricate vocal patterns and recounting of the Ramayana Epic featured in Balinese Kecak. This monograph serves as an informational performance guide for Monkey Chant that simplifies and resolves performance questions and issues. It provides a contextual setting for the work with a brief biography of Glenn Kotche, including his musical influences and inspiration for the composition. Balinese Kecak is examined as well as the Hindu Ramayana tale as it relates to Kotche’s composition, revealing the function for its compositional form. This document also clarifies and details preparatory procedures for collecting and building the unique instruments required, also detailing schematics for electronic audio equipment and setup. Lastly, there is an analysis of compositional style and form, offering optional solutions to performance obstacles.

The samango monkey (Cercopithecus mitis erythrarchus) is the southern most representative of the polytypic mitis group. The samango is also the only truly arboreal guenon to have radiated as far as 30°S. At southern latitudes a greater seasonality of climate and an attendant seasonal shift in food availability is expected to restrict the foraging strategy of the arboreal guenon. In the absence of arboreal congenerics and few frugivorous bird and bat species, the samango experiences a level of competitive release at Cape Vidal not found in other equatorially located mitis populations. In this thesis I examine and contrast the diet and feeding behaviour of the mitis species group. In this way I illustrate the consequences of seasonality of food abundance and competitive release on the foraging strategy of the samango, and provide an explanation for the unique distribution of the samango monkey as the only arboreal guenon in southern Africa. Despite seasonality in climate and abundance of food resources, my data show that, in general, samango monkeys at Cape Vidal are not food limited. For this reason Cape Vidal samangos have large troop sizes (25+), use small home ranges (15 ha) and have the highest density (2.02 ind/ha) of any C. mitis population researched to date. Fruit forms an important part of the diet year-round and therefore, energy and carbohydrate are abundant. There is very little intra-group aggression for food, although interindividual distances are greatest when feeding. There are age-sex differences in the diet, and adult males eat more fruit while females eat more leaves than other age-sex classes. The most important aspect of the feeding strategy of the samango is concerned with obtaining adequate protein in the diet, and throughout the range of the mitis group, populations differ most in feeding strategies used to secure protein-rich foods, such as young leaves, flowers and invertebrates. In this respect seasonal nutrient (protein) availability, rather than seasonality of food abundance per se, is the most limiting component of the forest environment. Unlike equatorial populations of mitis that derive most of their protein from insects, samangos are unable to adopt a similar strategy. Insects were available to the monkeys, and then only in low numbers, in the wet summer season at Cape Vidal. Samango monkeys, therefore, make greater use of a wider variety of plant items for protein acquisition. During the wet summer months, insects, flowers and young leaves are used by samangos to obtain sufficient protein for important reproductive activities, such as lactation, and initial growth of the infant. During the drier winter months only mature leaves and small quantities of young leaves are available as protein resources, consequently samangos use more mature leaf in the diet than other C. mitis populations. Adaptations of the gut and specialized gut microflora permit this high degree of folivory in the diet (Bruorton and Perrin 1988) of the samango monkey. This is a characteristic that does not appear to be shared with other arboreal guenons. In so far as protein is essential for reproduction, folivory in c. mitis has been important in permitting this arboreal guenon species-group to radiate into southern latitudes where protein is more seasonally available. The ability of samango monkeys to eat large quantities of leaves at anyone time, accounts for their unique position as the only arboreal guenon species in southern Africa. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1990.

Despite the existence of effective vaccines for both diseases, measles and poliomyelitis still cause significant worldwide morbidity and mortality. The live-attenuated measles and inactivated polio vaccines are both given using a standard needle and syringe injection. This method of delivery poses many problems for large-scale vaccination campaigns. Microneedles are micron-scale needles which have the potential to overcome many of these hurdles. In the first study, we showed that the measles vaccine could be successfully incorporated into a solid, metal microneedle system which induced potent neutralizing antibody titers after administration into cotton rats. This response was statistically identical to the same dose delivered using a subcutaneous injection. The second study focused on enhancing the stability of the measles vaccine after drying and long-term storage. Using a new assay developed from a measles virus variant engineered to encode for green fluorescent protein, it was determined that a combination of sucrose and threonine provided the highest stabilizing effect. Vaccine mixed with this solution retained more than 90% of its activity after 6 months of storage at 4°C and 25°C. The third study involved the incorporation of the measles vaccine into a dissolving microneedle patch. These patches were used to vaccinate rhesus macaques and the immune response was found to be statistically identical to the same dose delivered by syringe injection. Furthermore, after creation and storage, these patches retained 100% of their infectivity after 2 months at 4°C and 25°C. The final study attempted to create a dissolving microneedle patch containing a full dose of the inactivated polio vaccine. These patches were then used to deliver a full dose of IPV into the skin of a rhesus macaque. This delivery method produced neutralizing antibody titers to IPV type 1 and 2 that were statistically identical to the same dose delivered using a needle and syringe. Overall, these studies show that the microneedle patch was a safe, simple and effective method for measles and polio vaccination. This delivery platform has the potential to overcome many of the hurdles that currently stand in the way of measles elimination and polio eradication.

Thesis (M.S.)--University of Michigan, 1992.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1958. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (p. 92-94).

Thesis (Ph. D.)--University of Wisconsin, 1958. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.A.)--University of Wisconsin--Madison, 1970. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.A.)--University of Wisconsin--Madison, 1969. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (p. 138-144).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1963. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 149-156).