Le LIF (Leukemia Inhibitory factor), une cytokine de la famille de l’Interleukine 6, permet le maintien de la pluripotence des cellules souches embryonnaires murines (CSEm) in vitro. Dans le but de comprendre les mécanismes d’action du LIF dans ce modèle d’étude, une analyse sur puces à ADN a été réalisée et a permis d’identifier trois « signatures LIF » : les gènes « Pluri » (pour Pluripotence), dont le niveau d’expression relatif chute suite au retrait de cette cytokine, et deux catégories de gènes « Lifind » (pour LIF induit) dont le niveau d’expression relatif augmente suite à un ajout de LIF après une culture de 24 ou 48 heures sans cette cytokine. Nous avons mis au point des tests fonctionnels permettant d’étudier la fonction des gènes cibles du LIF dans notre modèle d’étude. Ainsi, nous avons mis en évidence le rôle d’un gène « Pluri », Mras/Rras3, une petite GTPase de la famille Ras, dans la régulation de l’expression d’une part de marqueurs de pluripotence, tels que Oct4 et Nanog et d’autre part de marqueurs de différenciation, tels que Lef1 et Fgf5. / LIF (Leukemia Inhibitory factor), a cytokine Interleukin 6 family, allows maintaining the pluripotency of murine embryonic stem cells (mESC) in vitro. To understand the mechanisms of action of the LIF in this model, a microarray analysis was conducted and identified three « signatures LIF » : the « Pluri » (for Pluripotency) genes, whose the relative level of expression falls following the withdrawal of this cytokine, and two classes of « Lifind » (for LIF induced) genes, whose the relative expression level increases as a result of LIF addition after a culture of 24 or 48 hours without this cytokine. We have developed functional tests to study the function of the target genes of LIF in our study model. Thus, we have investigated the role of a « Pluri » gene, Mras/Rras3, a small GTPase of the Ras family, in the regulation of the expression on the one hand of markers of pluripotency, such as Oct4 and Nanog, and on the other hand of differentiation markers, such as Lef1 and Fgf5.

<p>Important factors in manufacturing are quality and cost. Measuring machines play an important role for these fields. In order to meet higher demands on cost and accuracy, measuring machines can be constructed with weaker materials and increased mechanical flexibilities, and therefore there is a need to include the flexibilities in measuring machine models to obtain good performance. </p><p>The core theme in this thesis is modeling and idenfication of the physical parameters of drive mechanisms of a Brown&Sharpe Inc. Global A coordinate measuring machine. The approximation made is that the drive mechanisms can be described by a mass connected by springs, dampers and gear changes. It has been found that a one-spring model gives a reasonably good description of the studied CMM drive mechanism. The physical parameters of this model are identified using off-line algorithms. The algorithms are based on prediction error methods. For the off-line identification the MATLAB System Identification Toolbox and the bond graph representation is used. </p><p>The chosen model is then used for control. Traditional control and a Model-reference Adaptive System is derived and studied with the aim to increase the damping of CMM drive mechanisms. It is found that the adaptive system has very good disturbance rejection and can correct for drastic model errors. Another impact is that the damping of the studied drive mechanism can be increased with at least a factor of nine.</p>

Important factors in manufacturing are quality and cost. Measuring machines play an important role for these fields. In order to meet higher demands on cost and accuracy, measuring machines can be constructed with weaker materials and increased mechanical flexibilities, and therefore there is a need to include the flexibilities in measuring machine models to obtain good performance. The core theme in this thesis is modeling and idenfication of the physical parameters of drive mechanisms of a Brown&amp;Sharpe Inc. Global A coordinate measuring machine. The approximation made is that the drive mechanisms can be described by a mass connected by springs, dampers and gear changes. It has been found that a one-spring model gives a reasonably good description of the studied CMM drive mechanism. The physical parameters of this model are identified using off-line algorithms. The algorithms are based on prediction error methods. For the off-line identification the MATLAB System Identification Toolbox and the bond graph representation is used. The chosen model is then used for control. Traditional control and a Model-reference Adaptive System is derived and studied with the aim to increase the damping of CMM drive mechanisms. It is found that the adaptive system has very good disturbance rejection and can correct for drastic model errors. Another impact is that the damping of the studied drive mechanism can be increased with at least a factor of nine.

The goal of this thesis is to deal with comparison of algorithms in the field of sensorless control of permanent magnet synchronous motor. It is focused mainly on deterministic methods of estimation, verification of their dynamic responses and the influence of parasitic effects. Implementation of selected algortihms on DSP in fractional arithmetic with verification by Processor-in-the-loop method is described in second part. The last part describes use of algorithms on real drive.

Baigiamajame darbe sudarytas uždarosios asinchroninės bejutiklės vektoriškai valdomos pavaros imitacinis modelis ir ištirtos charakteristikos. Teorinėje darbo dalyje yra aptariami asinchroninių elektros pavarų privalumai bei šiose pavarose naudojami greičio jutikliai. Aprašomi stebiklių privalumai bei trūkumai, pagrindžiamas jų naudojimas asinchroninėse pavarose. Nagrinėjami bejutiklių elektros pavarų ypatumai, aprašomi vektorinio valdymo bendrieji principai bei aprašomi bejutiklėse vektoriškai valdomose pavarose naudojamų stebiklių modeliai. Pateikiami du skirtingi asinchroninių variklių matematiniai modeliai. Tiriamojoje dalyje parenkamas asinchroninio variklio modelis, tiriant abiejų imitacinių modelių dinamines greičio charakteristikas. Sudaromas stebiklio imitacinis modelis. Tiriamos stebiklio greičio dinaminės charakteristikos, sudaroma uždaroji greičio reguliavimo sistema su stebikliu. Analizuojamos uždarosios greičio reguliavimo sistemos greičio charakteristikos be apkrovos, su šuoline apkrova ir harmoniškai kintančia apkrova. Nagrinėjama sistemos stiprinimo koeficiento įtaka uždarosios greičio reguliavimo sistemos greičio charakteristikų pereinamiesiams procesams. Magistro darbas baigiamas tyrimo išvadomis, kuriose aptariamas darbo rezultatų realaus pritaikymo galimybės. Darbą sudaro 8 dalys: įvadas, žymėjimai, literatūros šaltinių analizė, tyrimo tikslas ir uždaviniai, teorinė dalis, tiriamoji dalis išvados ir pasiūlymai, literatūros šaltiniai. / The final master degree thesis presents sensorless vector controlled induction motor drive simulation model and characteristics. In the analytic part of master thesis advantages of induction motor drives and speed sensors are described. Advantages and disadvantages of speed estimators are presented and purpose of using them are proved. Peculiarities of sensorless motor drives, principles of vector control and models of speed estimators are analyzed. Two simulation models of induction motor are proposed. In the research part characteristics of induction motors are compared and motor model is chosen. Characteristics of open loop induction motor drive are investigated and simulation model of closed loop induction motor drive with speed estimator is designed. Characteristics of closed loop control system at no load, constant load and harmonic load are analyzed and influence of speed controller gain is considered. Thesis is closed with conclusions about designed system application in real projects. Structure: introduction, list of symbols, literature review, the study aims and objectives, the theoretical part, research part, conclusions and proposals, references.

Le travail présenté dans cette thèse a pour objectif d'apporter une contribution aux méthodes de commande et d'observation des machines asynchrones destinées à la traction électrique. Dans ce contexte, plusieurs algorithmes ont été développés et implémentés. Après une présentation rapide de la commande vectorielle classique, de nouvelles approches de commande non linéaire sont proposées : il s'agit plus précisément de la commande backstepping classique et sa variante avec action intégrale. Une deuxième partie est consacrée à l'observation et à l'estimation des paramètres et des états de la machine, basée sur des structures MRAS-modes glissants d'une part et sur des structures de filtrage synchrone d'autre part. Une analyse détaillée du problème de fonctionnement à basse vitesse nous a conduit à proposer une solution originale dans le cadre d'une commande sans capteur mécanique. Le problème de la dégradation du couple en survitesse a été traité par un algorithme de défluxage basé sur la conception d'un contrôleur de tension. Enfin, nous avons proposé un algorithme d'optimisation afin de minimiser les pertes dans l'ensemble Onduleur-Machine / The work presented in this thesis aims to contribute to the control and observation of the induction machines for electric traction. Several algorithms have been developed and implemented. After a fast presentation of the classical vector control, new approaches of non-linear control are proposed : the classical backstepping and integral backstepping. A second part deals with the observation and the estimation of parameters and states of the machine, based on MRAS-Sliding Mode structures on one hand and on synchronous filtering structures on the other hand. A detailed analysis of the operation at low speed led us to propose an original solution for a Sensorless control. The torque degradation in field weakening zone was treated by a voltage regulation controller. Finally, we proposed losses minimization algorithm for the Inverter-Machine set

This thesis presents different state estimation techniques for speed sensorlees field oriented control of induction motors. The theoretical basis of each algorithm is explained in detail and its performance is tested with simulations and experiments individually. First, a stochastical nonlinear state estimator, Extended Kalman Filter (EKF) is presented. The motor model designed for EKF application involves rotor speed, dq-axis rotor fluxes and dq-axis stator currents. Thus, using this observer the rotor speed and rotor fluxes are estimated simultaneously. Different from the widely accepted use of EKF, in which it is optimized for either steady-state or transient operations, here using adjustable noise level process algorithm the optimization of EKF has been done for both states / the steady-state and the transient-state of operations. Additionally, the measurement noise immunity of EKF is also investigated. Second, Unscented Kalman Filter (UKF), which is an updated version of EKF, is proposed as a state estimator for speed sensorless field oriented control of induction motors. UKF state update computations, different from EKF, are derivative free and they do not involve costly calculation of Jacobian matrices. Moreover, variance of each state is not assumed Gaussian, therefore a more realistic approach is provided by UKF. In this work, the superiority of UKF is shown in the state estimation of induction motor. Third, Model Reference Adaptive System is studied as a state estimator. Two different methods, back emf scheme and reactive power scheme, are applied to MRAS algorithm to estimate rotor speed. Finally, a flux estimator and an open-loop speed estimator combination is employed to observe stator-rotor fluxes, rotor-flux angle and rotor speed. In flux estimator, voltage model is assisted by current model via a closed-loop to compensate voltage model&rsquo / s disadvantages.

In recent years, multi-functional reconfigurable antennas (MRA) has attracted much attention in wireless communication. The reconfigurable antenna can adapt itself with changing system conditions, and it can provide different multi-functionalities which can give better system performances. Instead of using multiple antennas, a single reconfigurable antenna can provide the same performance and occupy less space. By using the parasitic layer technique, an antenna can be turned into a reconfigurable antenna. The main objective of this thesis is to study radiation pattern reconfiguration of the horn antenna by using the parasitic layer concept. The MRA consists of a single horn, dielectric loaded truncated pyramid (DLTP), and the parasitic layer. The antenna that is chosen in this thesis is the horn antenna because it provides high directivity. DLTP is used for magnification purpose. The results show that three modes of operations that provide better performances compared to the single horn antenna are achieved.

Neste trabalho é apresentado um estudo sobre o controle de velocidade sensorless aplicado ao Motor de Indução Trifásico (MIT) visando sobretudo, melhorar o desempenho da máquina de indução em aplicações na região de baixa frequência de operação. Para tanto, propõe-se uma abordagem utilizando a técnica de controle Direct Torque Control (DTC) com Space Vector Modulation (SVM) em conjunto com o Observador de Fluxo com Modelo de Tensão e Corrente (OFMTC), o que possibilita a estimação precisa da posição do vetor de fluxo do estator em baixa velocidade. Para a estimativa da velocidade do rotor é utilizado a estratégia Model Reference Adaptive System (MRAS) com mecanismo de adaptação fuzzy, a fim de se diminuir a sensibilidade aos parâmetros da máquina. O desempenho da estratégia de controle de velocidade sensorless proposta é testada em simulações com diferentes condições de operação buscando reproduzir alguns dos problemas encontrados em aplicações práticas, utilizando o software Matlab. Os resultados obtidos foram condizentes com resultados encontrados na literatura, comprovando o bom desempenho do sistema de controle de velocidade proposto. / This work deals with the sensorless speed control for Three-phase Induction Motor in order to improve its operation at low speed and low frequency. At first, a Direct Torque Control employing Space Vector Pulse with Modulation (SVPWM) based on Flux Observer (voltage and current model) is studied and evaluated due to its high accuracy of estimating the stator-flux at low speed. The Model Adaptive Reference (MRAS) with Fuzzy Controler is chosen in order to estimate the rotor speed. This choice is based on its capability of high performance during Three-phase Induction Motor operation even if the machines parameters and the load change during the operation. At last, some simulations with diferent operational conditions at low speed are carried out in order to test the sensorless control. The results show a good perfomance and agreed with the results shown in recent papers.

Neste trabalho é apresentado um estudo sobre o controle de velocidade sensorless aplicado ao Motor de Indução Trifásico (MIT) visando sobretudo, melhorar o desempenho da máquina de indução em aplicações na região de baixa frequência de operação. Para tanto, propõe-se uma abordagem utilizando a técnica de controle Direct Torque Control (DTC) com Space Vector Modulation (SVM) em conjunto com o Observador de Fluxo com Modelo de Tensão e Corrente (OFMTC), o que possibilita a estimação precisa da posição do vetor de fluxo do estator em baixa velocidade. Para a estimativa da velocidade do rotor é utilizado a estratégia Model Reference Adaptive System (MRAS) com mecanismo de adaptação fuzzy, a fim de se diminuir a sensibilidade aos parâmetros da máquina. O desempenho da estratégia de controle de velocidade sensorless proposta é testada em simulações com diferentes condições de operação buscando reproduzir alguns dos problemas encontrados em aplicações práticas, utilizando o software Matlab. Os resultados obtidos foram condizentes com resultados encontrados na literatura, comprovando o bom desempenho do sistema de controle de velocidade proposto. / This work deals with the sensorless speed control for Three-phase Induction Motor in order to improve its operation at low speed and low frequency. At first, a Direct Torque Control employing Space Vector Pulse with Modulation (SVPWM) based on Flux Observer (voltage and current model) is studied and evaluated due to its high accuracy of estimating the stator-flux at low speed. The Model Adaptive Reference (MRAS) with Fuzzy Controler is chosen in order to estimate the rotor speed. This choice is based on its capability of high performance during Three-phase Induction Motor operation even if the machines parameters and the load change during the operation. At last, some simulations with diferent operational conditions at low speed are carried out in order to test the sensorless control. The results show a good perfomance and agreed with the results shown in recent papers.