Version remaniée de: Diss.--Ruprecht-Karls-Universität Heidelberg, 1989.

Thesis (Ph. D.)--University of Oregon, 1999. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 329-354). Also available for download via the World Wide Web; free to University of Oregon users. Address: http://wwwlib.umi.com/cr/uoregon/fullcit?p9957574.

Made available in DSpace on 2016-08-29T15:33:46Z (GMT). No. of bitstreams: 1 tese_6279_Geane Oliveira de Lanes.pdf: 34557531 bytes, checksum: f89fdbcbc1ec3ef0a6393219b27c9a98 (MD5) Previous issue date: 2013-02-27 / O exoesqueleto e a musculatura da família de vespas parasitóides Bethylidae é revisado. Termos utilizados para outros grupos de Hymenoptera são revisados, e uma terminologia consensual é proposta. Caracteres externos são redescritos e correlacionados aos apódemas e músculos correspondentes; sua importância filogenética é discutida. 456 estruturas esqueléticas foram denominadas e definidas, das quais 133 são propostas ou redefinidas. 69 músculos são examinados e foram propostas homologias com aqueles presentes em outras famílias de Hymenoptera.

Made available in DSpace on 2016-08-29T15:38:09Z (GMT). No. of bitstreams: 1 tese_6282_Robson Rangel.pdf: 1513411 bytes, checksum: 6157d8d30829b5ecdeca60ba0a4ee590 (MD5) Previous issue date: 2013-02-26 / O cultivo da pimenteira-do-reino (Piper nigrum L.) para fins comerciais, no Espírito Santo, iniciou-se na década de 70, sendo hoje a cv. Bragantina a mais representativa nas lavouras capixabas. A pipericultura no Espirito Santo necessita urgente de estudos e pesquisas, para continuar a ser destaque de produtividade, com tecnologias apropriadas para a região. Neste sentido a qualidade das plantas matrizes inicia-se com a produção de mudas de excelência. Apesar da experiência dos viveiristas da região em produzirem mudas por estaquia, as etapas deste processo devem ser muito bem inspecionadas para garantir o máximo de eficiência. O estudo teve como objetivo investigar o sistema de produção de mudas de pimenteira-do-reino, inclusive a eficiência dos substratos utilizados nos viveiros. Foi solicitado ao Ministério da Agricultura Pecuária e Abastecimento (MAPA) a localização de viveiros em conformidade com a legislação pertinente e mediante as informações prestadas foram realizadas visitas técnicas a 11 viveiros, registradas por meio de um questionário. Coletaram-se amostras dos substratos utilizados na produção de mudas. As respostas dos questionários foram tabeladas e transformadas em gráficos de freqüência relativa para melhor contextualização da situação, e os substratos foram utilizados para montar um experimento fatorial 11(substratos) x 2 (presença e ausência de ácido-3- indolbutírico) visando avaliar o enraizamento de estacas de pimenteira-do-reino cultivar Bragantina, realizado em blocos ao acaso com 4 repetições de 16 estacas, na casa de vegetação localizada no Centro Universitário Norte do Espírito Santo, no Bairro Litorânio, no município de São Mateus-ES. Constatou-se que não existe uniformidade na produção de mudas, na infraestrutura dos viveiros e nas técnicas utilizadas. As diferenças estão relacionadas a origem das plantas matrizes, ao material propagativo, e ao manejo durante o crescimento das mudas, ficando confirmado, através de análises, diferença na composição química, física e biológica dos substratos, influenciadas principalmente pela diversidade da fonte de matéria orgânica e a utilização ou não de corretivos e adubações. Concluiu-se que, apesar de todos os viveiros serem registrados no Registro Nacional de Sementes e Mudas (RENASEM) e estarem em conformidade com as exigências legais em vigência, ainda é preciso unir informações e estudar as divergências para que a produção de mudas de pimenteira-do-reino tenha parâmetros técnicos consistentes. A qualidade do enraizamento de estacas de pimenteira-do-reino nos substratos utilizados pelos viveiristas, foi analisada estatisticamente por testes de médias (Tukey ou Scott-Knott a 5% de probabilidade), e constatou-se melhora significativa com a aplicação de ácido indolbutírico, ao tempo que observou-se diferença significativa na qualidade do enraizamento entre os substratos. Palavras-chave: Estaquia, viveiristas, substrato, lavoura, enraizamento e mudas

This thesis addresses the paucity of research on network mobility with a taxonomy and a quantitative comparison on a real test bed of existing and forthcoming network mobility solutions. Broadly, network mobility solutions can be divided into two broad categories, intra-domain and inter-domain solutions. The two are distinct enough to warrant separate solutions. Network mobility solutions can also be divided into four catogories according to their implementation, (i) Mobile IP-based solutions, (ii) Mobile IP Mobile Routing, (iii) intra-domain routing protocols, and (iv) Mobile IP Foreign Agent-based solutions. This latter division by implementation serves as a useful starting point for a discussion and study of these protocols. The qualitative and quantitive comparisons presented here yield two "winning" solutions, Mobile IP with Network Address Translation (NAT) and Mobile IP Mobile Routing, both based on Mobile IP, the draft standard for Internet mobility. The two were chosen because they are the only solutions fit for production networks, not necessarily because they are the best performing solutions. Indeed, nearly all of the other solutions are promising alternatives and some, such as mobile ad hoc network (MANET) routing protocols and the solutions based on the next generation Internet (IPv6) version of Mobile IP, are particularly so. One of the solutions uses a novel extension for Mobile IP, the Multiple Home Address extension, developed and specified in this thesis, that turns a Mobile IP mobile node into a mobile router able to support dynamically sizing mobile networks while using the existing Mobile IP infrastructure. / Master of Science

Dans ce travail nous étudions la complexité de certains fragments du mu-calcul selon deux points de vue: l’un syntaxique et l’autre topologique. Dans la première partie nous adoptons le point de vue syntaxique afin d'étudier le comportement du mu-calcul sur des classes restreintes de modèles. Parmi d'autres résultats, nous montrons en particulier que sur les modèles transitifs toute propriété définissable par une formule du mu-calcul est définissable par une formule sans alternance de points fixes. Pour ce qui concerne la perspective topologique, nous montrons d'abord que sur les modèles transitifs la logique modale correspond au fragment borélien du mu-calcul. Ensuite nous donnons une description effective des hiérarchies de Borel et de Wadge d'un sous-fragment sans alternance de cette logique sur les arbres binaires et vérifions que pour ce fragment les points de vue topologique et syntaxique coïncident. / In this work we study the complexity of some fragments of the modal mu-calculus from two points of view: the syntactical and the topological. In the first part of the dissertation we adopt the syntactical point of view in order to study the behavior of this formalism on some restricted classes of models. Among other results, we show that on transitive transition systems, every mu-formula is logically equivalent to an alternation free formula. For what concerns the topological point of view, we first prove that on transitive models, the modal logic is exactly the Borel fragment of the modal mu-calculus. Then we provide an effective description of the Borel and Wadge hierarchies of a sub-fragment of the alternation free fragment of the mu-calculus on binary trees. Finally we verify that for this fragment the syntactical point of view and topological point of view coincide.

Bacteriophage Mu exhibits low specificity for the 5 bp sequence it selects as its transposition target, but shows regional biases in its insertion choices. For example, Mu prefers AT-rich DNA in vitro, exhibits a 1000-fold bias in target preference within the E. coli chromosome, and avoids targets carrying Mu end sequences. The Mu transposase is responsible for recognition of the 5 bp target consensus, but depends on the accessory protein MuB for efficient target capture. MuB preferentially binds to AT-rich DNA, explaining this particular regional preference. We have uncovered opposing roles for MuB in target capture and integration. We show while MuB-bound AT-rich DNA is favored for integration, the bound DNA itself is refractory, and that transposition occurs adjacent to, but not within the bound region. We show that this property of MuB is likely responsible for immunity of Mu from self-integration, since MuB was found to be strongly bound within the Mu genome. Genome-wide analysis of MuB binding on the E. coli chromosome showed that Mu target preference is positively related to MuB binding profile, and that MuB binding is insulated by the nucleoid-associated protein Fis but not by transcription events. Since Fis binding to the chromosome responds to the frequency of A-tracts, a chromosome domain structure signal, Mu transposition must also respond to chromosome domain signals. Work in this dissertation has provided a new understanding of how MuB influences and controls Mu target choice, and of reciprocal interactions between a bacterial chromosome and a transposable element. / text

Mu is a temperate bacteriophage which infects Escherichia coli and several other Gram negative enteric bacteria. It is an extraordinary phage in several respects and has carved a special niche for itself both as a genetic tool and a paradigm in phage biology, almost rivaling phage lambda. It is also a predator that has adapted its hunting skills well in order to have an extraordinarily wide host range. While phage Mu finds a mention in almost every genetics textbook for several of its unique and well-studied characteristics, there are a few aspects of its biology that are far from understood. In this thesis, light has been shed on one such less understood feature of Mu biology, namely its anti-restriction function. The enigmatic mom gene of bacteriophage Mu is the center of this thesis work. Bacteriophages, through their sheer number and versatility of attack tactics, constitute an overwhelming threat to bacteria in the natural environment. While it is not always possible to completely prevent the entry of foreign DNA into the cell, it is in the interest of the bacterium to tame the xenogeneic DNA, whose expression may have adverse effects on bacterial fitness. Bacterial nucleoid associated proteins (NAPs) participate in chromosome structuring and global transcriptional regulation. Besides this canonical role, they furnish the job of regulating xenogeneic DNA as well. NAPs are known to regulate the expression of toxin-antitoxin modules, pathogenicity islands and other horizontally transferred DNA and have a profound role in regulating transposon dynamics and the lifestyle of many phages. Chapter 1 introduces the role of bacterial NAPs in silencing foreign DNA, especially after the DNA establishes itself in the host. This thesis examines the role of a bacterial NAP namely Fis in fine-tuning an immune evasion function of bacteriophage Mu. A general introduction to phage Mu and its host expansion strategies, with special focus on its DNA modification function is also presented. Owing to the various immune evasion strategies, phages often have an upper hand on their hosts in the ongoing evolutionary arms race. One such strategy is DNA modification which bacteriophages have evolved as a means to protect their genomes from restriction enzymes of the host. While most phages employ the commonplace methylation modification for their anti-restriction function, phage Mu employs an unusual acetamido modification, catalysed by its protein Mom. Mom modified DNA is refractory to several restriction enzymes from different bacterial species. However, the modification is toxic to the host and thus mom expression needs to be precisely regulated to prevent untimely expression. A crowded multifactorial regulatory circuit has evolved to ensure the expression of mom without jeopardizing the welfare of the bacterial host. Chapter 2 uncovers a new player in mom regulation. The study shows that the bacterial chromatin architectural protein Fis is a transcriptional repressor of mom promoter and that Fis mediates its repressive effect by denying access to RNA polymerase at mom promoter. Two distinct roles of Fis have been known previously in Mu biology. In addition to bringing about the overall downregulation of transposition events and transcription of early genes of phage Mu, Fis also stimulates tail fiber flipping by aiding the activity of a site-specific recombinase. The present study thus presents a novel facet of Fis function in Mu biology. While the regulation of mom has been a matter of intense investigation over the past few decades, most biochemical and structural aspects of the Mom protein per se have remained mysterious owing to the difficulties in cloning this toxic gene. Chapter 3 describes the expression, purification and biophysical characterization of Mom. A variety of techniques show Mom to be folded and dimeric in solution. SPR studies with Mom indicate its high affinity binding to DNA. Chapter 4 deals with the attempts to identify the elusive co-factor of Mom. To begin with, the in vivo activity of Mom was demonstrated by employing a simple plasmid cleavage assay based on the resistance of Mom modified DNA to certain restriction endonucleases. A variety of disparate in silico structure prediction tools such as I-TASSER, Robetta and PHYRE indicate Mom to be related to the GCN5-related N-acetyltransferase superfamily. Mutation of residues deemed important from this analysis indeed abolished or reduced Mom activity in vivo, validating the bioinformatics based prediction and shed light on the possible active site of Mom. However, acetyltransferases are not known to transfer acetamido groups. It was also necessary to establish beyond doubt, the chemical structure of the Mom modified nucleoside. High resolution mass spectrometry data showed the modification to be acetamido, corroborating the earlier sole report on this aspect. Based on the biochemical reactions that acetyl coenzyme A is known to participate in, it is difficult to explain the involvement of acetyl coenzyme A in acetamido addition. Notwithstanding the converging predictions of different bioinformatics tools, caution is recommended when inferring function from structurally similar family members. It is possible that a different chemistry might have converged on the same (acetyltransferase) fold, given that none of the known pathways utilizing acetyl coenzyme A can explain the Mom modification. Several likely candidates such as carboxy-SAM, glyoxylic acid and glycine were also tested for being donors of the two carbon entity transferred on adenine by Mom. Since these candidates tested negative in our genetic assays, a genome-wide genetic screen was subsequently devised to identify the host genes involved in mom modification. The assay exploited the phenotype of lethality associated with overexpression of Mom in E. coli in order to screen for mutations in the host genome that rescued the toxicity. However, the survivors which were obtained in this assay had emerged through mutations in the mom gene rather than abrogation of the co-factor synthesis pathway of the host. The results point at two possibilities: (i) utilization of essential gene(s) or (ii) existence of redundant pathways for the Mom modification reaction. Chapter 5 is an account of our attempts to trace the lineage of mom and its regulatory region, employing updated DNA and protein sequence databases. Despite the selective advantage conferred on the phage by the anti-restriction function of mom, in many Mu-like phages, mom is either absent or substituted with methyltransferases. However, in Mu-like genomes that do encode mom, in spite of a significant overall sequence divergence from Mu, the core elements of the mom regulatory circuit seem to have either co-evolved or have been selectively conserved. Although Mu appears to be unique in the possession of a regulatory circuit tailored for the purpose of mom regulation, recently discovered Mu-like genomes show that different types of regulatory features evolved several times in closely related genomes. It is very likely that a toxic gene like mom has earned its place in the phage genome by carrying along with itself a baggage of regulatory elements. Failure to sustain sufficient regulatory pressure may trigger the loss or replacement of the advantageous but potentially lethal mom function.

La facilité de déploiement et la flexibilité des technologies sans fil ont permis une évolution considérable des normes IEEE 802.11. L'arrivée de la fibre optique à la maison a également motivé des études de recherche pour accroître les débits et la capacité. Dans ce contexte, la dernière norme IEEE 802.11ac ratifiée en 2014 a normalisé de nouvelles technologies pour atteindre des débits maximaux au-dessus de 1Obit/s. En particulier, 802 llac propose le Multiple Input Multiple Output Multi-Utilisateurs (MIMO-MU) et le Transmit Beamforming (TxBF). Ces technologies d'antennes intelligentes supposent un diagramme d'antenne d'émission qui dépend de la position des utilisateurs et des caractéristiques du canal de propagation multi-trajets correspondant. Cependant, les outils d'ingénierie et de planification radio actuels simulent une carte de couverture fixe et indépendante de tout traitement d'antennes adaptatives côté émission. Cette thèse étudie l'impact de ces techniques MIMO-MU et TxBF et propose des solutions adaptées pour l'ingénierie radio et l'optimisation du système ainsi que pour les outils de planification radio. En effet, la première partie de cette thèse consiste à affiner les gains de capacité en identifiant les configurations et les paramètres système optimaux. La deuxième partie aborde les gains de beamforming en termes du gain sur le rapport signal à bruit (SNR) et sur la réduction du niveau d'interférence pour avoir une modélisation réaliste de la couverture radio. / The deployment facility and flexibility of wireless technologies have allowed considerable evolution of IEEE 802 .11 standards. The arrival of the optical fiber to the home has further motivated the research studies to increase data rates and capacity. ln this context, the latest IEEE 802.l lac standard ratified in 2014 proposes new technologies to achieve maximum data rates above 1 Gbit/s. ln particular, 802.l lac includes the Multi-User Multiple Input Multiple Output (MU-MIMO) and Transmit Beamforming (TxBF). These smart antenna technologies assume a variable transmit antenna pattern in function of the users' position and the corresponding multipath propagation channel characteristics. However, the actual radio planning tools give a fixed coverage map in dependent of any adaptive or smart antenna processing. This thesis studies the impact of the MU-MIMO and TxBF techniques on radio engineering, system optimization as well as radio planning tools. Indeed, the first part of this thesis deals with refining the sum rate capacity gains by identifying interesting system configurations and optimal system parameters. The second part addresses the beamforming gains in terms of the Signal-to-Noise Ratio (SNR) gain and interference level to have realistic radio coverage modeling.

The goal of this dissertation was two fold: 1) To relate dopamine responses in the ventral striatum to ethanol preference and consumption, and 2) to investigate the role of the mu opioid receptors in this ethanol induced dopamine release in the ventral striatum. First a two bottle choice experiment established that a substrain of C57BL/6 mice (C57BL/6NCrl) had significantly less preference for and consumption of ethanol than a second substrain of mouse based on the same background (C57BL6/J). The C57BL/6 strain has been extensively used in alcohol drinking studies and is well known for it’s propensity to consume alcohol over water. To determine if differences in ventral striatal dopamine response vii could contribute to this variability in drinking behavior, we characterized the dopamine response in both substrains of mice after intraperitoneal injections of 1.0, 2.0 or 3.0 g/kg ethanol or saline. We found that the acute intraperitoneal ethanol injections in naïve mice caused a significant elevation in dopamine in both substrains at all three doses with a significant difference between substrains at the two highest alcohol doses. Therefore, ethanol induced dopamine release in the ventral striatum may contribute to ethanol preference and consumption. Next, we investigated the effect of acute intraperitoneal ethanol injections on naïve mu opioid receptor knockout mice and in mice pretreated with a mu opioid receptor antagonist. The mice used were all established on the C57BL/6J background. We found that ventral striatal dopamine response was similar in these mice after 1.0, 2.0 and 3.0 g/kg intraperitoneal ethanol injections compared to appropriate controls. As both gene deletion and pharmacological blockade of the mu opioid receptor did not affect ethanol stimulated dopamine release, it points to the conclusion that this receptor may not play a significant role in ethanol induced ventral striatal dopamine release. / text