The following four broad aims were addressed in this study. (1) To ascertain whether the characteristics of the benthic macroinvertebrate assemblages within the different nearshore marine habitat types identified by Valesini et al. (2003) on the lower west coast of Australia differ significantly, and whether the pattern of those spatial differences matches those among the environmental characteristics that were used to distinguish those habitat types; (2) To develop a quantitative approach for classifying nearshore habitats in estuarine waters that employs readily-available data for a range of enduring environmental characteristics, and to use that approach to classify the various habitat types present in nearshore waters of the Swan-Canning Estuary on the lower west coast of Australia; (3) To test the hypothesis that the characteristics of the benthic macroinvertebrate assemblages in the in the Swan-Canning Estuary differ significantly among nearshore habitat types, and that the pattern of those differences matches that among the environmental characteristics used to distinguish those habitat types and (4) To test the hypothesis that, as a result of environmental changes in the Swan-Canning Estuary, the characteristics of the benthic macroinvertebrate assemblages at various habitats in this estuary in 1986/7 differ from those in 2003/4. To address the first aim, benthic macroinvertebrates were sampled seasonally for one year in the subtidal waters and intertidal zone (upper and lower swash zones) at the six nearshore habitat types that were identified by Valesini et al. (2003) on the lower west coast of Australia. The habitat types, which differed mainly in the extent of their exposure to wave activity and whether seagrass and/or nearshore reefs were present, had been distinguished quantitatively using values for a suite of seven statistically-selected enduring environmental characteristics. The faunal samples yielded a total of 121 species representing eight phyla, among which the Polychaeta, Malacostraca and Bivalvia were the most speciose classes and contributed ~ 38, 23 and 10%, respectively, to the total number of individuals. The total number of species and mean density of macroinvertebrates was far greater at the most protected habitat type (1), which also contained dense beds of seagrass, than at any other habitat type, i.e. 70 species and 209.2 individuals 0.1 m-2, compared to 32 species and 36.9 individuals 0.1 m-2 at the most exposed habitat type (6), which had a substrate comprised only of sand. Differences among habitat type influenced the benthic macroinvertebrate species composition to a greater extent than differences among either zones or seasons. Significantly different faunal compositions were detected among those latter two factors only at the most protected habitat type. The faunal assemblage at habitat type 1 was clearly the most distinct from those at the other five habitat types, particularly in the subtidal zone (R-statistics=0.642-0.831, p=0.1%), and was typified by five abundant polychaete species that were adapted to deposit-feeding. In contrast, the fauna at habitat type 6 was typified by four crustacean species and a species of bivalve and polychaete, whose mobility and tough external surface facilitated their survival and feeding in those turbulent waters. The extents of the differences in species composition among the six habitat types was significantly matched with that among the suite of enduring environmental characteristics that distinguished those habitat types, particularly in the case of the subtidal zone (Rho=0.676). Such results indicated that the environmental variables used to distinguish the nearshore habitat types could be used to reliably predict the types of benthic macroinvertebrate species likely to occur at any site along the lower west coast of Australia. The above biological validation of the nearshore marine habitat classification scheme developed by Valesini et al. (2003) provided the justification for the approach to the second broad aim of this study, namely to develop a quantitative scheme for classifying habitat types in the Swan-Canning Estuary. This approach was similar to that employed by Valesini et al. (2003) in that it considers that differences among habitat types are well reflected by differences in a suite of enduring environmental variables. However, it improves on that earlier method by employing a completely objective and quantitative approach. Thus, a large number of environmentally-diverse nearshore sites (102) were initially selected throughout the Swan-Canning Estuary and a suite of 13 enduring environmental variables quantified at each using remotely-sensed images of the estuary in a Geographic Information System. Such variables were chosen to reflect either (i) the type of substrate and submerged vegetation present, (ii) the extent of exposure to wave action or (iii) the location of the site within the estuary with respect to its vicinity to marine and fresh water sources. These data were then subjected to the CLUSTER routine and associated SIMPROF procedure in the PRIMER v6 multivariate statistical package to quantitatively identify those groups of sites that did not differ significantly in their environmental characteristics, and thus represented habitat types. Eighteen habitat types were identified, which were shown to well reflect spatial differences in a suite of non-enduring water quality and sediment characteristics that were measured in situ at a range of estuarine sites during both summer and winter in 2005 (Rho=0.683 and 0.740, respectively, p=0.1%). However, those latter environmental characteristics required far more time in the field and laboratory to quantify than the enduring variables used to identify the habitat types. Benthic macroinvertebrates were sampled during summer and winter in 2005 in the shallow subtidal regions (~1 m depth) at sites representing eight of the habitat types identified in the Swan-Canning Estuary. These samples contained a total of 51 and 36 species during summer and winter, respectively, and, in both seasons, represented nine phyla, namely Annelida, Crustacea, Mollusca, Sipuncula, Nematoda, Platyhelminthes, Cnidaria, Uniramia and Nemertea. The compositions of the benthic macroinvertebrate assemblages differed significantly among habitat types and, to a similar extent, between seasons (Global R-statistic=0.408 and 0.409, respectively, p=0.1%). However, the spatial differences were considerable greater in winter than in summer (Global R-statistic=0.536 vs 0.280, p=0.1%), presumably due to the greater spatial variation in particular non-enduring in situ environmental characteristics, such as redox depth and salinity. While the number of species, overall density and taxonomic distinctness of benthic macroinvertebrates also differed significantly among habitats, those variables differed to a greater extent between seasons, being greater in winter than in summer. While the measures of taxonomic distinctness tended to be greater at habitat types located in the lower to middle reaches, i.e. habitat types 6, 7, 9, 10, 13 and 18, than the upper reaches i.e. habitat types 1 and 3, the number of species and overall density reflected this trend only during winter. During summer, the mean numbers of species at habitat types 1, 3, 6 and 10 (3.4-6.0) were significantly lower than those at habitat types 7, 13, and 18 (8.8-10.9), whereas the overall density of benthic macroinvertebrates was far greater at habitat type 7 (32260 individuals 0.1 m-2)than at any other habitat type in this season (3135-18552 individuals 0.1 m-2). Overall, the greatest differences in assemblage composition occurred between those at habitat types 1 and 18 (R-statistic=0.669, p=0.1%), which were located in the uppermost region of the estuary and the lower reaches of the basin, respectively, and differed to the greatest extent in their enduring environmental characteristics. The assemblage at habitat type 1, and also that at habitat type 3, located just downstream, were relatively distinct from those at all other habitat types, particularly during winter (R-statistics=0.666-0.993, p=0.1%). The fauna at the first of these habitat types was relatively depauperate, containing low numbers of species and densities, and was characterised by the polychaetes Leitoscoloplos normalis and Ceratonereis aequisetis and the bivalve Arthritica semen. The assemblage at habitat type 3 was also characterised by those three species and the amphipod Paracorophium minor and the polychaete Boccardiella limnicola. In contrast, the assemblage at habitat type 18 was characterised by a more diverse assemblage, i.e. the polychaetes Capitella capitata, C. aequisetis, L. normalis and Pseudopolydora kempi, the amphipods, Grandidierella propodentata and Corophium minor and the bivalve Sanguinolaria biradiata. The number of species was among the highest at this habitat type during both seasons, which was also reflected in the high taxonomic diversity, and the overall density was the highest in winter and second highest in summer. Despite the above faunal differences, those between assemblages at habitat types 7 and 9, which were both located in the basin of the Swan-Canning Estuary, were similar in magnitude to those that occurred between pairs of habitat types located in two different regions of the estuary. Although both habitat types 7 and 9 were characterised by a similar suite of species, i.e. Oligochaete spp., C. aequisetis, C. capitata, C. minor, G. propodentata, L. normalis, and S. biradiata, the substantial differences in assemblage composition between these habitat types in both summer and winter (R-statistics=0.570 and 0.725, respectively) was due to marked differences in the relative contributions of each of these species. Significant and strong correlations were shown to exist in both summer and winter between the pattern of differences in the benthic macroinvertebrate assemblages among habitat types and that among the enduring environmental characteristics used to identify those habitat types (Rho=0.625 and 0.825, respectively, p=0.1%). Furthermore, these correlations were greater than those obtained between the benthic macroinvertebrate fauna and any combination of the non-enduring environmental characteristics (i.e. water quality and sediment parameters) recorded in situ at each habitat type (Rho=0.508 and 0.824, in summer and winter, respectively, p=o.1%). This demonstrates the greater capacity of surrogate enduring environmental characteristics to account for differences in the range of variables that may influence the distribution of benthic invertebrate fauna. Thus, the lists of characteristic benthic macroinvertebrate taxa produced for each of the eight habitat types studied in the Swan-Canning Estuary provide a reliable benchmark by which to gauge any future changes in those fauna. Moreover, these results indicate that the above habitat classification scheme can be used to reliably predict the types of benthic macroinvertebrate fauna that are likely to occur at any nearshore site of interest in this estuarine system. The final component of this study showed that the benthic macroinvertebrate assemblages at four sites in the middle reaches of the Swan-Canning Estuary in 2003/4 differed significantly from those recorded at the same sites in 1986/7. Such differences were reflected in (1) changes in the relative densities of a suite of ten species that were responsible for distinguishing the faunas in these two periods, (2) the absence of 22 rare species in 2003/4 (i.e. 42% of the number of species recorded in 1986/7), (3) the presence of 17 new species in 2003/4, including an abundant polychaete that is likely to have been introduced and (4) a far greater extent of seasonal variation in the number of species and densities of benthic macroinvertebrates in 2003/4. Such changes are likely to be related to lower sediment oxygen levels in certain seasons in 2003/4, as well as an altered hydrological regime due to increased temperatures and decreased rainfall in that more recent period. The fact that these changes have occurred within the Swan-Canning Estuary highlights the need for effective management tools, such as the habitat classification scheme and associated faunal survey undertaken in this study. Such data will provide a sound basis by which to examine the ways in which fauna vary spatially within the system, and allow for the establishment of comprehensive benchmarks for detecting future changes.

For various reasons, existing methods for the assessment of aquatic pollution do not always adequately address the way in which contaminants affect receiving environments and their component ecosystems. The main advantage of biological assessment over the measurements of physical and chemical aspects of water quality is that biota provide an integrated response to all prevailing influences in their environment. Biological assessment protocols have been developed for a range of test organisms, from bacteria to mammals using measurement from molecular biomarkers to indicators at the population or community level of organisation. Macroinvertebrates in particular have been popular for ecological assessment of habitat and water quality because they are small and straight forward to sample and identify using relatively simple and inexpensive equipment and readily available taxonomic keys. However, various biological assessment techniques also have their limitations. Field-based assessment of biological communities does not provide direct evidence to determine underlying causal relationships, while laboratory or mesocosm toxicity tests are criticised for their limited ability to extrapolate to natural field conditions. To help bridge the gap, this thesis aims to investigate the efficacy of using caged macroinvertebrates in situ to assess the ecological condition of aquatic environments, and whether a causal relationship can be established when macroinvertebrates are deployed in situ at sites known to have impaired water quality. Endpoints employed in this thesis include survival, measurements of morphology (as a surrogate for growth) and condition and, for trials assessing sites that receive mine drainage, the tissue concentration of certain trace metals. Development of an in situ approach to water quality monitoring and assessment will potentially provide methods for use by resource managers, community groups and aquatic researchers that are less expensive and faster to run than existing methods and will complement other approaches employed in the assessment of water quality. In situ testing of water quality using macroinvertebrates requires the collection, handling, caging, deployment and retrieval of test organisms at sites of suspected pollutant impact. As such procedural factors may affect test organisms and potentially confound their responses, it is important to consider and understand as many of these factors as possible. Aquatic macroinvertebrates held in finer mesh cages had larger heads than in coarser mesh cages. This was likely due to increased substrate available for growth of epilithon and periphyton on which the caged organisms could graze. Caging density had no effect on amphipod mortality over the trial period, however, individuals held at higher densities increased in size (as indicated by longer dorsal lengths) more than those held at lower or intermediate densities. Temporary storage of test organisms in laboratory aquaria may facilitate the collection of abundances required for in situ trials, however, tanked individuals were smaller and had lower biomasses than individuals collected and deployed immediately. While this is likely to result from differences in feeding during the storage period, it is also possible that tank storage and the ?double handling? deleteriously affected them, or reduced their tolerance. The effects of transplanting macroinvertebrates between sites varied considerably depending on the characteristics of "source" and "transplant" sites. Certain taxa suffered marked mortality within 24 hours even at their source site, indicating an adverse effect of the caging itself, or perhaps via the change in food, shelter or microclimate which could potentially render them unsuitable as test organisms in caging studies. Other taxa did not differ in survival or body size when relocated between sites, with some evidence of increased growth at sites dissimilar from their source site. In general, organisms relocated to sites that are "similar" to their source environment performed less well at the transplant site. However, organisms transplanted to "dissimilar" sites were found to be bigger than those caged and deployed back to the source site. When employed to assess known pollution scenarios in and around Canberra, macroinvertebrate responses were, in some instances, able to be linked to specific environmental parameters or combinations thereof. In Case Study 1, findings varied in relation to the response endpoint being examined, and between test species, although concentrations of metals were significantly higher in tissue of macroinvertebrates deployed at the impact site downstream of the abandoned Captains Flat mine and increased with time exposed. In Case Study 2, freshwater shrimp suffered significant mortality within 24 hours of deployment at the impact sites, with larger individuals more susceptible at sites receiving urban stormwater runoff. While various biological effects were most closely correlated with ammonia concentrations at the site, different body size endpoints were affected in opposite ways. In Case Study 3, body size endpoints for one test organism varied consistently with respect to site and time factors, but none of the changes could be linked to any of the environmental data collected. Response variables for a different test species also indicated significant effects arising from both deployment site and time, however, each endpoint responded in a different way to the treatment factors, and aligned with different combinations of environmental data. In general, linking of macroinvertebrate responses with environmental data was difficult because of the high variability in the environmental data. However, it was further complicated by the mismatch in the level of replication between the two datasets. As a consequence of this, the macroinvertebrate data had to be collapsed to a lower level for comparison with the environmental data, resulting in a loss of natural variability and analytical power. Since only the strongest treatment effects, which could be detected above the background "noise", were detected and modelled against the environmental data, it is possible that other "cause" and "effect" relationships may have been overlooked. From these results, it is clear that many macroinvertebrate taxa are suitable for use as bioindicators in in situ trials, but that criteria used for selection of test species should definitely include more than just impact-sensitivity and abundance. However, there are several aspects associated with the experimental set up of field-based protocols involving caged macroinvertebrates that may limit their usefulness as a rapid and reliable bioassessment tool, and need to be considered when designing and undertaking these kinds of trials. It is also apparent that choice of endpoint can greatly influence conclusions, with detection of treatment effects reported in this thesis varying greatly depending on which morphological endpoint was examined. This study clearly demonstrated that there may be significant difficulties in establishing causal relationships between environmental data and biotic responses of macroinvertebrates deployed under field conditions. However, it has also shown that deployment of caged macroinvertebrates in situ may assist in the determination of biological effects arising from impaired water quality, which can then serve as the basis for more focussed laboratory or mesocosm studies in which environmental conditions can be more readily controlled or monitored.

Nutrients, periphyton and macroinvertebrates were sampled every two months for one year to assess the effects of treated sewage effluent on the Thredbo River and to compare changes in chemical concentrations and the invertebrate community since the introduction of nutrient removal on the Thredbo sewage treatment plant. Chemically, the impact of the effluent was minor and was reduced from levels measured before the addition of nutrient removal to the Thredbo sewage treatment plant. However, the reduction may be the result of higher flows during the study period. Biologically there was no indication of nutrient enrichment during June, August and October, with differences occurring between physically dissimilar sites irrespective of position relative to the sewage effluent inflow. Large increases in periphyton and macroinvertebrates occurred in December, February and April at both open sites and those downstream of the sewage outflow. Previous studies found increases in invertebrate abundance and richness immediately downstream of the sewage inflow, but the impact was greater and extended further downstream than found in this study. Strong correlations between total phosphorus and periphyton biomass and chlorophyll-a indicate phosphorus was a limiting nutrient. Higher periphyton biomass and chlorophyll-a at all sites during the warmer months (especially at open sites with an even cobble substratum), throughout the year, indicates light, temperature and substratum type were as important as phosphorus in controlling algal growth. Low algal biomass during the high flow month of October also indicates the importance of water velocity in controlling periphyton biomass. The structure of the macroinvertebrate community was noticeably different at sites downstream of the effluent discharge, with the exception of the most downstream site, when compared to the upstream sites. The occurrence of high macroinvertebrate numbers and species richness at sites with high periphyton growth and the strong positive correlation between invertebrate grazers and periphyton measurements shows a dependent relationship between invertebrates and periphyton growth. Therefore, the physical factors of light, temperature and substratum characteristics of particular sites in the Thredbo River, may be as important in controlling the benthic community as the levels of nutrient inputs from sewage.

This thesis aims to improve our understanding of how riverine ecosystems respond to dam removal. Riverine and particularly riparian ecosystems are among the most variable and important features of all landscapes. They connect landscape elements both longitudinally and laterally, and are governed by processes such as flooding, erosion and deposition that create dynamic, diverse and heterogeneous habitats. In fact, riparian zones are among the world’s most species-rich habitats. Worldwide there are millions of dams that fragment stream and river systems, regulate flows and degrade ecosystems. Dams impact freshwater, marine and terrestrial ecosystems and threaten biodiversity by disrupting organism movements and energy flows in the landscape. An important upstream effect of dams is inundation of habitats and development of new shorelines around impounded areas. Effects downstream of dams are mainly caused by changed hydrological regimes and retention of organic and inorganic materials in reservoirs, leading to reduced transport and dispersal of for example seeds to reaches downstream. The removal of dams create expectations that biota will eventually recover. We have studied a number of dam removal projects in Sweden. Our experimental results showed that following dam removal, newly exposed soils in former impoundments were rapidly colonized by pre-removal species. Their species richness increased slightly with time and their species composition indicated a slow change towards that in the reference site. In addition, the vegetation in formerly impounded areas showed a direction of change from lentic riparian plants (high proportion of aquatics) towards lotic ones, consisting of native perennials typical of free-flowing streams. We also found that the apprehensions that former impoundments would turn into pools of mud did not come true; in fact, a process towards more pristine channel morphology was observed. After removal there was erosion and downstream transport of sediment. We found only minor effects on macroinvertebrate communities. For example, a few species decreased over the years, suggesting that dam removal in itself might cause a temporary disturbance. This highlights the importance of long-term studies after dam removal, and also the importance of comparisons with pre-removal conditions and stretches unaffected by dams. Thorough documentation of executed dam removal projects and distribution of the results and experiences are tremendously important in the planning process of future decommissioning projects. Also, our experiences have taught us that in order to attain a successful dam removal it is important to involve stakeholders such as non-governmental organizations and local inhabitants in the process.

In streams, wood provides protection, food and substrate for benthic macroinvertebrates, which leads a higher density and diversity of the benthic fauna compared to streams without wood. To examine wood and its effect on drift of macroinvertebrates an experiment was done in 18 sections of six semi-natural streams where fine woody debris (Salix sp.) was added to half of the sections (1-2 per stream). The purpose was to investigate possible differences in density and diversity of macroinvertebrates aswell as to investigate if there was any effect of location, upstream or downstream, in the streams. The results showed no significant effect of woody debris on total drift. The lack of an effect is probably related to the high abundance of predators (fish, brown trout) and the time of the day when the samples were taken. There was a significant effect of time on total drift; with more drift in September than in October. An effect of time was also found for the taxon Diptera (Chironomidae not included), with drift decreasing with temperature over the season. / Ved i ett vattendrag ger skydd, föda och substrat åt bentiska makroevertebrater, vilket leder till en högre täthet och diversitet av makroevertebrater än i vattendrag utan ved. För att undersöka vedens inverkan på makroevertebraternas drift utfördes ett experiment i sammanlagt 18 inhägnader i semi-naturliga bäckar där hälften av inhägnaderna behandlades med fin ved (Salix sp.). Syftet var att undersöka eventuella skillnader i täthet och diversitet av makroevertebrater samt om det fanns en skillnad i läge, uppströms och nedströms i bäckarna. Resultatet visade ingen signifikant effekt av ved med avseende på den totala driften. Det fanns heller ingen signifikant effekt av ved på tätheten i enskilda på ved än utan. De bakomliggande faktorerna till varför ingen signifikant effekt av ved visades är troligtvis den höga tätheten av predatorer (öring) och tidpunkten då proverna togs. Det fanns dock en signifikant effekt av tid gällande den totala driften, i september var det mer drift än i oktober. Det fanns även en signifikant effekt av tiden på Diptera (alla Diptera taxa förutom Chironomidae). Detta tros vara på grund av den sjunkande temperaturen mellan månaderna.

Η οδηγία της Ευρωπαϊκής Ένωσης 2000/60 για τα νερά θέτει το πλαίσιο δράσης όλων των κρατών μελών της Ευρωπαϊκής Ένωσης για σωστή διαχείριση των υδάτινων πόρων τους προκειμένου να επιτευχθεί «καλή» οικολογική ποιότητα των επιφανειακών και υπόγειων υδάτων τους μέχρι το έτος 2015 και να αποτραπεί η περαιτέρω υποβάθμισή τους, με στόχο να διασφαλισθεί η υγιής λειτουργία των υδρόβιων οικοσυστημάτων. Προκειμένου να εκτιμηθεί η οικολογική ποιότητα των υδάτων των ποταμών Πείρου-Παραπείρου και Βουραϊκού, πραγματοποιήθηκαν δειγματοληψίες και εν συνεχεία χημικές, υδρομορφολογικές και βιολογικές αναλύσεις σε έντεκα θέσεις, επιλεγμένες με συγκεκριμένα κριτήρια κατά μήκος αυτών. Συγκεκριμένα, η συλλογή των δειγμάτων πραγματοποιήθηκε και τις τέσσερις εποχές του έτους 2006, ενώ στους ποταμούς Πείρο και Παραπείρο υλοποιήθηκε μια επιπλέον δειγματοληψία κατά την άνοιξη του έτους 2007. Η υδρομορφολογική ανάλυση πραγματοποιήθηκε ακολουθώντας τη μεθοδολογία River Habitat Survey, ενώ η βιολογική ανάλυση περιελάμβανε τη συλλογή δειγμάτων βενθικών μακροασπον-δύλων σύμφωνα με τη μεθοδολογία STAR AQEM. Παράλληλα, χρησιμοποιήθηκαν τεχνικές στατιστικής ανάλυσης προκειμένου να εντοπιστούν οι σημαντικότερες περιβαλλοντικές παράμετροι που επηρεάζουν τις βιοκοινότητες των μακροασπον-δύλων. Για τον υπολογισμό της οικολογικής ποιότητας εφαρμόστηκε η μεθοδολο-γία REFCOND προκειμένου να εκτιμηθεί αυτή με βάση την επιμέρους συμβολή των χημικών, υδρομορφολογικών και βιολογικών παραμέτρων στη διαμόρφωσή της. Σύμφωνα με τα αποτελέσματα της έρευνας περισσότερο του ήμισυ του μήκους των ποταμών Πείρου-Παραπείρου και των 3/5 του μήκους του ποταμού Βουραϊκού δεν ικανοποιούν τις απαιτήσεις της οδηγίας 2000/60/ΕΕ, εκτιμώμενη η οικολογική τους ποιότητα από «φτωχή» έως «μέτρια». Για τους Πείρο και Παραπείρο, η ποιοτική υποβάθμιση αποδίδεται σε αγροτικές και κτηνοτροφικές δραστηριότητες καθώς και στην παρουσία αστικών και βιομηχανικών αποβλήτων στις κατάντη θέσεις. Η αντίστοιχη του Βουραϊκού, εν μέρει οφείλεται σε αγροκτηνοτροφικές δραστηριότη-τες αλλά κυρίως στην παρουσία σημαντικών ποσοτήτων τυροκομικών αποβλήτων. Επιπλέον, τα ευρήματα στο συγκεκριμένο ποτάμι πιστοποιούν τον καθοριστικό ρόλο της παρόχθιας βλάστησης στην απορρύπανση των υδάτων των ποταμών. Κατόπιν των ανωτέρω κρίνεται επιτακτική η ανάγκη να ληφθούν τα ενδεδειγμένα μέτρα προστασίας των νερών των ποταμών από τα παντοειδή απόβλητα, προκειμένου να αναβαθμιστεί η ποιότητά τους με τελικό στόχο να ικανοποιηθούν οι απαιτήσεις της οδηγίας 2000/60 για διασφάλιση «καλής» οικολογικής ποιότητας μέχρι το έτος 2015. / One of the main issues of the EU Water Framework Directive 2000/60 is the achievement of “good” ecological status for the surface waters by the year 2015. All European countries are obliged to assess the ecological quality of their surface water bodies and classify them into a five-quality class system, with a final purpose to ensure “good” status for Europe’s water bodies and prevent their further deterioration. Eleven sites located in the rivers Peiros - Parapeiros and Vouraikos (Western Greece), were sampled seasonally and analyzed using physicochemical, hydromorphological and biological data, in order to classify the water quality according to the aforementioned directive. Physicochemical classification was performed using the Nutrient Classification System, while the habitat quality was estimated by applying the River Habitat Survey methodology. Biological sampling was performed by application of the STAR AQEM methodology, while the ecological classification was achieved by utilizing the “REFCOND guidance for the relative roles of the physicochemical, hydromorphological and biological quality elements”. Various multivariate techniques (Canonical Correspondence Analysis, Cluster Analysis and MDS) revealed the most important environmental factors that affected the macroinvertebrate communities. According to the results of the study, half length of the rivers Peiros-Parapeiros and the 3/5 of the river Vouraikos were found not to fullfil the demands of the WFD, with their quality being assessed from “moderate” to “poor”. Agriculture, urbanization and hydromorphological alteration were the main factors that contributed to the water quality degradation of the rivers Peiros and Parapeiros, while the presence of dairy wastewaters has been assessed as the main reason for the quality degradation of Vouraikos river. Moreover, the results of the study revealed the valuable role of the riparian vegetation in absorbing a large part of the incoming pollution. Finally, the results reveal the obligation for focused actions to be taken for monitoring and improvement of water quality, in order to meet the demands of the Water Framework Directive 2000/60/EU for “good” ecological quality, by the year 2015.

Lielupės upių baseinų rajono 2001 – 2004 m. upių monitoringo hidromorfologinių, bendrųjų – cheminių ir biologinių duomenų sąryšių analizei naudotas Spirmeno koreliacijos koeficientas ir paprasta bei daugialypė tiesinė regresija. Nustatyta, kad upių debitas buvo glaudžiai susijęs su bendrojo azoto ir HCO3– jonų koncentracijų pokyčiais. Upių tarša biogeninėmis medžiagomis iki 61,4 proc. sumažino deguonies koncentraciją vandenyje. Vandens rūgštingumo pokyčiai net 71,8 proc. padidino Fe jonų koncentraciją vandenyje. Gauti glaudūs Ca2+, Mg2+ ir HCO3– jonų koncentracijų tarpusavio ryšiai parodė karstinių reiškinių įtaką Lielupės baseino upių vandens kokybei. Chironomidae rūšių gausumas buvo geras upių taršos biogeninėmis medžiagomis indikatorius, tačiau BI ir DIUF indeksai buvo jautresni šių junginių kiekiams. DIUF indeksas buvo jautresnis Ephemeroptera taksonų pokyčiams, tačiau Trichoptera gausumo svyravimus geriau atspindėjo BI indeksas. Nustatytas makrozoobentoso ir Oligochaeta ir Chironomidae taksonų gausumo priklausomybės modelis paaiškino net 99,7 proc. makrozoobentoso gausumo variacijos, taigi, tiriamuoju laikotarpiu Lielupės baseino upėse vyravo mažai taršai jautrūs bentofaunos taksonai. / The goal of this study was to find relationships between some biological, chemical and hydro-morphological variables in the rivers of the Lielupe basin in 2001 – 2004 period. Spearman correlation, simple and multiple stepwise regression analysis were used. Close relations between river discharge and total nitrogen, HCO3– ions concentrations were established. Rivers pollution by biogenic matters was affected the loss of dissolved oxygen concentration until 61,4 %. Drastic increase of the concentration of Fe was characterized by low pH. Close relations between Ca2+, Mg2+ and HCO3– concentrations showed that the karst processes were determined water quality in the rivers of the Lielupe basin. Abundance of the Chironomidae species was good indicator of rivers water pollution by biogenic matters, but BI and DIUF indices were more sensitive to concentrations of these compounds. Comparison of sensibility of BI and DIUF indices to abundance of Ephemeroptera and Trichoptera species showed that DIUF index was more sensitive to abundance of Ephemeroptera species, but BI index was more sensitive to abundance of Trichoptera species. Established model, which was characterized the dependence between abundance of macroinvertebrates and abundance of Oligochaeta and Chironomidae taxons, was explained 99,7 % of the variation of the abundance of macroinvertebrates, thus benthic organisms which are less sensitive to pollutants were dominants in the rivers of the Lielupe basin in investigated... [to full text]

Climate change will affect all freshwater ecosystems via both direct physiological and indirect, biologically-mediated effects. Small lakes (< 10 ha) numerically dominate the Boreal Shield and represent an important habitat for aquatic biota. Small, shallow lakes are particularly responsive to climate-induced changes in thermal structure. Furthermore, biological interactions may be particularly important in small lakes where space, habitat heterogeneity, and thermal refugia are limited. Therefore, it is critical to understand and predict the consequences of climate change for community dynamics in small Boreal Shield lakes. Using 10 years of monitoring data and a field experiment I tested for differences in crustacean zooplankton community structure between warm and cool lake habitats. I classified years from a small, shallow Boreal Shield lake as ‘warm’ or ‘cool’ based on several characteristics of lake thermal structure. Since macroinvertebrates are often the main predators in small, shallow lakes, I further tested for potential interactions between lake thermal structure and spatially-dependent macroinvertebrate predation using in situ mesocosms. Body sizes of two ubiquitous crustacean zooplankton taxa, Leptodiaptomus minutus and Bosmina spp., were reduced in warm years, but no differences in abundance or diversity were detected at the annual scale. In contrast, in 15d enclosure experiments, crustacean zooplankton abundance and calanoid copepodid body size were reduced by the vertically-migrating predator Chaoborus punctipennis, but only in warm isothermal conditions. Zooplankton lowered their daytime depth distribution to avoid the surface-orienting notonectid predator, Buenoa macrotibialis, regardless of thermal habitat. No predation effect was detected in a hot (25ºC) isothermal habitat where both Chaoborus and notonectids were likely heat-stressed. Differences in abundance effects between the enclosure and monitoring data are likely due to the scales at which the analyses were conducted. Over short timescales predator-prey dynamics depended on lake thermal structure. However, over annual timescales zooplankton response was averaged across periods of seasonal change in thermal structure and biological processes, which may dampen the short-term effects associated with strong predation in isothermal conditions. Therefore, the importance of macroinvertebrate predators in regulating crustacean zooplankton community structure may increase if small lakes become progressively more isothermal with future climate change. / Thesis (Master, Biology) -- Queen's University, 2009-03-31 13:29:35.975

A study of the aquatic invertebrate communities from two locations (Location 1 and Location 2) within the Matapouri catchment in Northland, New Zealand, was conducted to assess community structure in differing local-scale habitats. Four data collection methods were utilised generating 33,058 adult or larval invertebrates. The sampling methods comprised benthic kick-sampling, sticky trapping, light trapping, and emergence trapping. For the sticky trapping and light trapping, sampling was carried out at three different sites (Sites 1–3) within each location. The sites were situated within three habitat types; native forest, native forest-fringe, and raupo wetland. Emergence trapping also commenced within the three sites, at both locations, but was discontinued after two months, due to the equipment being destroyed by consecutive flooding events (method described in Appendix 1). Benthic sampling was carried out within the Forest and Forest-fringe habitats. Benthic sampling, sticky trapping, and light trapping were carried out following a monthly schedule between June and November 2005. Conductivity, pH, and water temperature measurements were taken concurrently with benthic sampling on a monthly basis, while water velocity and substrate measurements were taken once to assist in habitat characterisation. Overall, 71 taxa were recorded by benthic sampling over the six month period, with a mean of approximately 30 taxa per site per month. In comparison with similar studies elsewhere in New Zealand, a figure of around 30 taxa per sample was high. The benthic macroinvertebrate fauna at all sites was dominated by Trichoptera (19 taxa), Diptera (16 taxa) and Ephemeroptera (10 taxa). This pattern of diversity is similar to that reported in other New Zealand studies. However, in contrast to previous studies, the leptophlebiid mayfly genus Deleatidium was not numerically dominant over the rest of the community, and other leptophlebiid genera (Acanthophlebia, Atalophlebioides, Mauiulus and Zephlebia) were equally represented, possibly reflecting niche partitioning between the groups. The genus Nesameletus was not recorded at any site, despite being one of the core mayfly species in New Zealand streams. The rare mayfly Isothraulus abditus was recorded at one of the forest locations. There are no published records of this species from Northland. Although acknowledged as another of the core New Zealand benthic taxa, the hydropsychid caddisfly Aoteapsyche was not recorded during the study. However, another hydropsychid, Orthopsyche, was commonly recorded, and these may be filling a similar niche to the Aoteapsyche genus. In contrast to the Trichoptera, Diptera, and Ephemeroptera, the Plecoptera fauna was relatively depauperate, probably reflecting the warmer climate of the region and lack of temperature-buffered spring-fed streams. Surprisingly, Zelandobius, a core New Zealand genus, was absent but is regularly recorded in Northland. A species of conservation interest, Spaniocercoides watti, currently recognised as a Northland endemic, was recorded in low numbers. There were no apparent trends in diversity or abundance of benthic invertebrates over time. Also, there were no significant differences in species diversity between the two locations. However, in many cases, taxa were more abundant at Location 2. This may have been due to steeper gradients at Location 2, and the consequent effects on substrate size and streambed stability, as all other physical factors appeared similar between locations. Although several significant differences of individual benthic taxa were recorded, no broad effect of habitat (sites) on species diversity was observable. However, at Location 2, abundances were significantly higher at Site 3 (Forest) compared to Site 2 (Forest-fringe). The reasons were uncertain, but may be attributed to higher retention of allochthonous organic materials, trapped by in-stream cover and larger substrates. Investigations of adult stages by sticky traps supported benthic results recording community compositions and abundances dominated by Trichoptera and Diptera. Plecoptera were poorly represented. Location 2 recorded higher abundances of taxa, particularly Ephemeroptera and Plecoptera. Investigations of adult stages by light traps however did not produce any statistically significant differences in abundances between sites, between locations, or between sites across locations, and it is believed to be due to limited sampling replication combined with some biases of light trapping. This study indicates that the aquatic invertebrate community at Matapouri is diverse but also reasonably representative. Several rare or uncommon insects inhabit the catchment. It is therefore important that Iwi and the local Landcare Group, who invited and supported this research, together with the Department of Conservation, continue their efforts in protecting these areas. The resident fauna have the capacity to restock areas downstream, which are intended to be improved and restored through sediment control and riparian management.

A study of the aquatic invertebrate communities from two locations (Location 1 and Location 2) within the Matapouri catchment in Northland, New Zealand, was conducted to assess community structure in differing local-scale habitats. Four data collection methods were utilised generating 33,058 adult or larval invertebrates. The sampling methods comprised benthic kick-sampling, sticky trapping, light trapping, and emergence trapping. For the sticky trapping and light trapping, sampling was carried out at three different sites (Sites 1–3) within each location. The sites were situated within three habitat types; native forest, native forest-fringe, and raupo wetland. Emergence trapping also commenced within the three sites, at both locations, but was discontinued after two months, due to the equipment being destroyed by consecutive flooding events (method described in Appendix 1). Benthic sampling was carried out within the Forest and Forest-fringe habitats. Benthic sampling, sticky trapping, and light trapping were carried out following a monthly schedule between June and November 2005. Conductivity, pH, and water temperature measurements were taken concurrently with benthic sampling on a monthly basis, while water velocity and substrate measurements were taken once to assist in habitat characterisation. Overall, 71 taxa were recorded by benthic sampling over the six month period, with a mean of approximately 30 taxa per site per month. In comparison with similar studies elsewhere in New Zealand, a figure of around 30 taxa per sample was high. The benthic macroinvertebrate fauna at all sites was dominated by Trichoptera (19 taxa), Diptera (16 taxa) and Ephemeroptera (10 taxa). This pattern of diversity is similar to that reported in other New Zealand studies. However, in contrast to previous studies, the leptophlebiid mayfly genus Deleatidium was not numerically dominant over the rest of the community, and other leptophlebiid genera (Acanthophlebia, Atalophlebioides, Mauiulus and Zephlebia) were equally represented, possibly reflecting niche partitioning between the groups. The genus Nesameletus was not recorded at any site, despite being one of the core mayfly species in New Zealand streams. The rare mayfly Isothraulus abditus was recorded at one of the forest locations. There are no published records of this species from Northland. Although acknowledged as another of the core New Zealand benthic taxa, the hydropsychid caddisfly Aoteapsyche was not recorded during the study. However, another hydropsychid, Orthopsyche, was commonly recorded, and these may be filling a similar niche to the Aoteapsyche genus. In contrast to the Trichoptera, Diptera, and Ephemeroptera, the Plecoptera fauna was relatively depauperate, probably reflecting the warmer climate of the region and lack of temperature-buffered spring-fed streams. Surprisingly, Zelandobius, a core New Zealand genus, was absent but is regularly recorded in Northland. A species of conservation interest, Spaniocercoides watti, currently recognised as a Northland endemic, was recorded in low numbers. There were no apparent trends in diversity or abundance of benthic invertebrates over time. Also, there were no significant differences in species diversity between the two locations. However, in many cases, taxa were more abundant at Location 2. This may have been due to steeper gradients at Location 2, and the consequent effects on substrate size and streambed stability, as all other physical factors appeared similar between locations. Although several significant differences of individual benthic taxa were recorded, no broad effect of habitat (sites) on species diversity was observable. However, at Location 2, abundances were significantly higher at Site 3 (Forest) compared to Site 2 (Forest-fringe). The reasons were uncertain, but may be attributed to higher retention of allochthonous organic materials, trapped by in-stream cover and larger substrates. Investigations of adult stages by sticky traps supported benthic results recording community compositions and abundances dominated by Trichoptera and Diptera. Plecoptera were poorly represented. Location 2 recorded higher abundances of taxa, particularly Ephemeroptera and Plecoptera. Investigations of adult stages by light traps however did not produce any statistically significant differences in abundances between sites, between locations, or between sites across locations, and it is believed to be due to limited sampling replication combined with some biases of light trapping. This study indicates that the aquatic invertebrate community at Matapouri is diverse but also reasonably representative. Several rare or uncommon insects inhabit the catchment. It is therefore important that Iwi and the local Landcare Group, who invited and supported this research, together with the Department of Conservation, continue their efforts in protecting these areas. The resident fauna have the capacity to restock areas downstream, which are intended to be improved and restored through sediment control and riparian management.