Recently, there has been an increase in marine mammal mortalities, most commonly Atlantic bottlenose dolphins, Tursiops truncatus, which is an alarming indication of the health status of the marine ecosystem. Studies have demonstrated that some free-ranging dolphins exhibit a suppressed immune system possibly because of exposure to contaminants or infectious microorganisms. However, this research has been limited due to a lack of commercially available marine-specific antibodies. Therefore, the first chapter of this thesis aims to identify cross-reactive terrestrial-specific antibodies that could be used to phenotype and compare the immune cell populations of dolphins under human care and free-ranging dolphins. The second chapter aims to utilize terrestrial-specific growth factors and dendritic cell (DC) surface markers to generate, characterize, and compare ex vivo DCs from peripheral blood mononuclear cells (PBMCs) of dolphins under human care and free-ranging dolphins. In summary, I have identified differences within the PBMCs and ex vivo generated DCs of dolphins under human care and free-ranging dolphins that could potentially shed light on the impact of environmental contaminants and infectious microorganisms on immune cells which could lead to increased morbidity and mortality. / Includes bibliography. / Dissertation (Ph.D.)--Florida Atlantic University, 2015. / FAU Electronic Theses and Dissertations Collection

In this thesis, research by Crum and Mao [J. Acoust. Soc. Am. 99, 2898--2907 (1996)] and Houser, Howard, and Ridgway [J. Theor. Biol. 213, 183--195 (2001)] is extended by numerically investigating bubble growth (initial radius of 10 microns) during rectified diffusion for gas supersaturations up to 300% by using the Fyrillas-Szeri equation [J. Fluid Mech. 277, 381--407 (1994)]. Bubble growth is simulated for a range of frequencies (100 Hz to 10 kHz), sound pressure levels (205 dB to 215 dB re 1 micropascal), and gas supersaturations (150% to 300%). Simulations are presented for continuous and monofrequency excitation, repeated tone bursts, and pulsed frequency-modulated waveforms. The potential for bubble growth to occur in marine mammals is also considered. For the parameters considered, static diffusion becomes the dominant growth mechanism as supersaturation is increased, bubble growth is frequency independent away from bubble resonance, and bubble growth due to duty-cycle excitation can be modeled as an effective continuous source with a reduced sound pressure level. A more accurate model of in vivo marine mammal tissue is required to determine if rectified diffusion can trigger bubble growth at the levels predicted in this thesis. / text

Approved for public release, distribution unlimited / This study sought to experimentally quantify the sonar performance of omni-directional receivers as a means to passively detect vocalizing Odontocetes. To accomplish this objective, controlled experiments using a calibrated mid-frequency sound source were conducted on the San Clemente Island Underwater Range (SCIUR) in July 2002. Six Odontocete signals were selected for transmission: 2 orca and 2 pilot whale whistles, and sperm whale and Risso's dolphin clicks. Several hundred iterations of each signal were broadcast at stations 300 m to 12,000 m from the range's moored, three-element array. Statistical analyses were performed on the output of an energy and matched filter detector to quantify detection probability (P(D)) and range limits as a function of false alarm rate (P(FA)), signal type, and signal to noise ratio (SNR). The matched filter was generally the superior performer, detecting the orca2 and pilot1 whistles beyond 5000 m with a 90% P(D), 1% P(FA), and source level (SL) of 140 dB re 1 æPa. For the same conditions, the orca1 and sperm whale calls were detected at 1500 m, but the pilot2 and Risso's dolphin signals were not detected at the peak realized SNR of (-2) dB. The energy detector had no detections with a 90% P(D) and 1% P(FA) at this (-2) dB SNR, but all signals except one orca whistle were detectable beyond 1000 m with a 50% P(D) and 1% P(FA). The sperm whale was the exceptional energy detector performer, with detection ranges exceeding 7 km (140 dB re 1 æPa SL) at the 50% P(D) and 1% P(FA) / Lieutenant Commander, United States Navy

<p>Sea turtles, marine mammals and sea birds are vulnerable to higher mortality rates as a direct function of incidental capture (bycatch) in marine fisheries. Their migratory behavior exposes them to multiple fishing gear types and fishing practices and efforts to understand the rates of interaction between these taxa and fishing necessarily entails analysis of data over large spatial areas (ocean-basin) and multiple types of fishing activities. The acquisition the requisite data, however, requires considerable resources and many regions in the world are data-poor with respect to bycatch, including the Wider Caribbean Region (WCR) in the west central Atlantic Ocean basin. This dissertation presents the results of multiple strategies used to assess sea turtle, marine mammal and seabird bycatch in the WCR, with a particular focus on sea turtle bycatch. The research incorporated a synthetic review of the literature, expert consultation, statistical techniques, and geospatial analyses to assess the bycatch seascape for the region. I conclude that sea turtle bycatch in the WRC is significantly linked to turtle rookeries, especially those on the continental land mass and in the southern section of the Caribbean basin, in large part because of the near shore artisanal nature of the fisheries and the importance of these habitats for foraging and reproduction. The limited information on marine mammal bycatch does not permit robust inferences, but it clearly identifies threats to at least one vulnerable marine mammal species, the tucuxi (Sotalia fluviatilis). Information on seabird bycatch was even more limited; the most vulnerable seabird populations occur in the higher latitudes (temperate zones) while the seabird populations in the WCR face significant threats from habitat loss and over-exploitation. This dissertation proposes specific recommendations for improving and advancing the information base for a regional, ecosystem-level management and mitigation of bycatch.</p> / Dissertation

Tuna-dolphin-bird feeding assemblages are unique to the Eastern Tropical Pacific Ocean (ETP). These multiple species groups are believed to forage together in response to the physical properties of the near surface ocean as these constrain the distribution of prey. In the Galapagos Marine Reserve (GMR), intra-annual and interannual changes affect the properties of the water column, inducing mesoscale and fine scale temporal variability. Four three-week oceanographic surveys took place, in September 2008, April 2009, October 2009, and September 2010, between the coast of Ecuador and the Galapagos Islands and one small boat survey took place in June 2010 within the GMR. Marine mammal surveys were conducted during daylight hours and Conductivity, Temperature, Depth (CTD) sensor casts were taken throughout the survey. Data were analyzed to determine the types of water masses present and the strength and depth of the thermocline layer. These data were compared with the sightings of marine mammals, bird feeding groups, and tuna-dolphin-bird assemblages. Additionally, these data were used to predict where tuna would be likely to associate with dolphin groups. Results show Equatorial Surface Water was the dominant water mass throughout the archipelago, regardless of season or ENSO index. High salinity, cold water west of Isla Isabela indicated topographic upwelling of the Equatorial Undercurrent. Tropical Surface Waters from the Panama Current were detected north of the Equatorial Front to the east of the islands. Obvious changes in the water column properties were observed between El Niño and La Niña events in the GMR. Most mixed groups were sighted west and south of Isla Isabela during the four oceanographic surveys, as well as north and west of Isla San Cristobal in June 2010. Most sightings were in cool, high salinity waters, and high chlorophyll concentrations. There were a greater number of sightings during the April 2009 survey (ENSO-neutral conditions) than during any of the three fall surveys. Additionally, tuna-dolphin-bird groups were more likely to be seen near Isla Isabela, with the majority of them sighted during the April 2009 survey and a few sighted in each of the September 2008 and October 2009 surveys. No tuna-dolphin-bird groups were sighted during the September 2010 surveys. Results show that the presence and location of these multi-species groups may be controlled by the inter-annual cycles, the intra-annual cycles, or a combination of both types of changes seen within the Galapagos.

Thesis (M.S.)--University of North Carolina Wilmington, 2009. / Title from PDF title page (January 14, 2010) Includes bibliographical references (p. 82-94)

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [110]-122).

Thesis (M. Sc.), Memorial University of Newfoundland, 1999. / Bibliography: p. 169-179.

Thesis (Ph. D.)--University of Adelaide, 2005. / Title from PDF title page (viewed on Feb. 3, 2006). Includes bibliographical references.

A floating object's drift is governed by its buoyancy, shape, and the wind, waves and currents it experiences. Here, I develop a drift modelling framework for marine mammal carcases in coastal waters. The resulting models were run forwards and backwards in time to provide insights into strategies for environmental monitoring under two scenarios. The first explored the beach search options for carcases resulting from potentially fatal collisions between tidal-stream turbines and marine mammals. The second applied the reverse problem for known-location mass strandings to highlight potential at-sea mortality sites. The drift properties of carcase-like objects were assessed in at-sea experiments. Wave transport was found to be greater than Stokes drift alone and in a complex coastal area could not be represented by a downwind multiplier as many previous models have assumed. A high resolution unstructured grid wave model was set up to complement existing wind and current models for the West Coast of Scotland, and these components were combined to build a carcase drift model. In the forward case, from tidal turbine locations, the drift model showed a wide spread of potential stranding sites, suggesting monitoring a limited number of beaches is unlikely to be fruitful. However, selecting beaches in response to immediate wind direction would improve efficiency. Stranding locations alone can only provide evidence of turbine interactions if the number of animals affected is large. In the reverse case, when applied to a mass stranding in Chile, the drift model showed the ability to exclude areas of origin, even though it could not pinpoint an exact mortality site. This work advances understanding of wave transport of surface floating objects, of carcase drift modelling, and of the feasibility of strandings monitoring. The decomposition rate of carcases is a source of uncertainty in the model where further work should be undertaken.