¡@The Port of Kaohsiung is an international commercial port, situated in the geographic center of Southeast Asia, linking China as a key hub. It links Japan and Korea to the north, and the Emerging ASEAN Market ¡V 10 nations in the Association of Southeast Asian Nations down south. There is no doubt that Port of Kaohsiung is now playing the key role as a worldwide Transshipment Center. Though there are many logistic related firms surrounded by the Port of Kaohsiung, most of them are running Container Storage and Freight Distribution business only. Lacking big logistic center of cold chain, marine products cannot be processed efficiently after unloading from refrigerators on ships. These seafood, needed to be packaging and exporting after processing, must be unloaded at general cargo berths and then moved to the frozen warehouses outside the harbor. ¡@This research applies a qualitative method of focus group interview. Through the comments given by VIP, professionals, and other members of focus groups in the selected firms, this research was hence capable of analyzing the problem and policy of creating a logistic center of seafood cold chain in the Kaohsiung Free Trade Zone. ¡@Kaohsiung is a main fishing port with numerous large ocean vessels and plenty marine products, it cannot be too far to assume that the creation of the logistic center for the cold chain would have huge contribution to attract customer, reduce operation cost, and enrich the competitive niches of related industry. As a result, the center will ultimately help to develop the ocean fishing business and to increase the trading volume of Kaohsiung. ¡@Finally, the conclusion of this research is expected to be applied in practice, all the issues found and suggestions made are for the references of both public and private sectors to help establishing the logistic center for cold chain as the ultimate goal of the research.

Organismos marinhos são reconhecidos por apresentarem potencial elevado para a produção de produtos naturais com estruturas moleculares únicas. Neste âmbito marinho, os fungos endofíticos são considerados como uma fonte promissora de substâncias bioativas, apresentando moléculas oriundas do metabolismo secundário mais significativas biologicamente do que aquelas produzidas por seus hospedeiros. Dentro deste contexto, este trabalho teve como objetivo principal a avaliação dos perfis químico e biológico do fungo endofítico Aspergillus unguis isolado da alga Palmaria decipiens, proveniente da Ilha Robert, Antártica. Para análise dos metabólitos secundários produzidos por A. unguis foram utilizados métodos cromatográficos como cromatografia em camada delgada (CCD) e cromatografia líquida de alta eficiência (CLAE), bem como técnicas espectroscópicas e espectrométricas de RMN 1-D e 2-D, CL-EM, CG-EM, inclusive para elucidação estrutural das substâncias isoladas. Todos os dados obtidos foram comparados com aqueles descritos na literatura e/ou com aqueles apresentados no software MarinLit® e o Dicionário de Produtos Naturais. Foram isoladas três substâncias do fungo estudado: Nidulina (DEP-1), pico 4-fração 8 (DEP-2) e pico 6-fração 8 (DEP-3). Todas são moléculas da classe das depsidonas, cuja nidulina já é uma molécula reportada na literatura. As outras duas são estruturas inéditas na literatura até o presente momento, uma vez que ainda não foram encontrados dados possibilitando a comparação com moléculas conhecidas. Concomitantemente, foram realizados experimentos para avaliação da atividade biológica de extratos, frações e substância isolada. As substâncias DEP-2 e DEP-3 foram submetidas para avaliação do potencial leishmanicida. As promastigotas IC50 para DEP-2 foram 68,1 ± 8,1, com índice de seletividade de 5,7, enquanto para os amastigotas o valor foi de 55,9 ± 0,6, com índice de seletividade 6,9, em comparação com os controles Amfotericina B com IC50 promastigota 3,5 ± 0,03 com índice de seletividade 6,6 e IC50 amastigotas 5,3 ± 0,2, índice de seletividade 4, 6 e Pentamidina com IC50 promastigotas 29,9 ± 0,08, índice de seletividade 1.2 e amastigotas IC50 18,2 ± 0,6, 1,9. Para DEP-3, o IC50 para promastigotas foi de 69,6 ± 0,7, com índice de seletividade 1,2. Considerando que a IC50 para amastigotas foi de 55,0 ± 0,2, com índice de seletividade 1,6, em comparação com os controles Amfotericina B com IC50 promastigote 3,48 ± 0,03, com índice de seletividade 6,6 e IC50 amastigotas 5,3 ± 0,2, índice de seletividade 4,6. Pentamidina com promastigotas IC50 29,9 ± 0,08, índice de seletividade 1,2 e amastigotas IC50 18,2 ± 0,6; índice de seletividade 1,9. O DEP-1 foi submetido para a avaliação da fotoestabilidade e fototoxicidade como considerado foto estável com uma queda na fotoestabilidade inferior a 0,1. E a DEP-2 e a DEP-3 estavam em mistura foram enviadas para análise da atividade microbiana e concluiu-se que há atividade sinérgica contra S. aureus, uma vez que o DEP-2 sozinha apresentou CIM de 100 ?g / mL e em mistura foi obtido CIM de 6,25 ?g / mL. / Marine organisms are recognized by present high potential for the production of natural products with unique molecular structures. In this context, the endophytic fungi are considered as a promising source of bioactive compounds, showing molecules from the secondary metabolism more biologically meaningful than those produced by their hosts. Within this context, this work had as main objective the evaluation of the chemical and biological profiles of the endophytic fungus Aspergillus unguis isolated from the alga Palmaria decipiens, from Robert Island, Antarctica. For analysis of secondary metabolites produced by A. unguis chromatographic methods were used as thin-layer chromatography (CCD) and high performance liquid chromatography (HPLC), as well as spectroscopic and spectrophotometric techniques such as NMR 1-D and 2-D, LC-MS, GC-MS, including for structural elucidation of isolated substances. All the data obtained were compared with those described in the literature and/or with those presented in the MarinLit ® software and the Dictionary of Natural Products. From the studied fungi, three substances were isolated: Niduline (DEP-1), peak-4 fraction-8 (DEP-2) and peak-6 fraction-8 (DEP-3). All molecules present depsidone skeleton, whose Niduline is the most molecule reported in the literature. The other two are unpublished structures in the literature up to the present time, since until now it has not yet been found enabling data for comparison with known molecules. At the same time, experiments were performed to evaluate the biological activity of extracts, fractions and isolated substance. The substances DEP-2 and DEP-3 were submitted to test to evaluate the leishmanicidal potential. The promastigotes IC50 for DEP-2 was 68.1 ± 8.1, with selectivity index was 5.7; while for amastigotes the value was 55.9 ± 0.6, with selectivity index 6.9, compared with controls Amphotericin B with IC50 promastigote 3,5 ± 0,03 with selectivity index 6.6, and IC50 amastigotes 5.3 ± 0.2; selectivity index 4.6 and Pentamidine with IC50 promastigotes 29.9 ±0.08, selectivity index 1.2 and IC50 amastigotes 18.2 ± 0.6, selectivity index 1.9. For DEP-3 the IC50 for promastigotes was 69.6±0.7, with selectivity index 1.2; whereas the IC50 for amastigotes was 55.0±0.2, with selectivity index 1.6, compared with controls Amphotericin B with IC50 promastigote 3.48 ± 0.03, with selectivity index 6.6 and IC50 amastigotes 5.3 ± 0.2, selectivity index 4.6. Pentamidine with IC50 promastigotes 29.9 ±0.08, selectivity index 1.2 and IC50 amastigotes 18.2 ± 0.6, selectivity index 1.9. DEP-1 was submitted for evaluation of photostability and phototoxicity as considered photostable with a drop in photostability of less than 0.1. Since the compounds DEP-2 and DEP-3 were sent in a mixture for analysis of the microbial activity and it was concluded that there is a synergistic activity against S. aureus, since DEP-2 alone had a MIC of 100 ?g/mL and in mixture obtained a MIC of 6.25 ?g/mL.

This master thesis delves into the design and aesthetics of a specific product in the marine industry, a stabiliser fin. The thesis is in collaboration with the company Humphree which develops and sells one of these fins. The Humphree all-speed fins are mounted on yachts to stabilise the vessel while cruising as well as at zero speed. The fins provide the user with stability and increase the comfort for the whole crew while optimising fuel efficiency. As this fin withstands huge loads and forces the fin should be trustworthy and express so as well. As of today, these criteria might not be fully fulfilled. This is part of the first aim of this thesis, “How can the design be updated to align with customer’s desires?”. Humphree focus is to stabilise marine vessels with their two main products, the stabiliser fins, and their Interceptors. As Humphree grows, the company should explore new markets within the marine area and create products with a common thread, which is a part of the second aim of this thesis “How can the product better showcase its capabilities?”. As the world faces environmental complications and risks, Humphree wants to do their part in the area. The marine market is already seen as a villain in this area, especially in the private sector. This is something that could, and should, be improved as a big part of the world’s population seeks more environmental products. This is investigated in the final aim, “How can the environmental impact of the product be reduced?”. Throughout the pilot study, the principles of Human-Centred design were applied to enhance the quality of the research and facilitate the identification of correlations between information instances. The primary objective was to conduct a pilot study to identify the future requirements for stabiliser fins. This involved primarily interviewing assemblers and people from Humphree, as well as benchmarking and lastly a design review was held to determine the necessary technical solutions. Secondly, a new concept of the fin should be made according to the findings from the pilot study. The thesis resulted in that the new concept should have a material change to aluminium instead of carbon fibre. The aluminium enhances the fin’s robustness and enables repairability, thus improving its environmental friendliness. Design adjustments to the exterior were made to align with Humphree’s product design, optimize mounting efficiency and enhance the fin´s robust appearance. However, the design changes were small as the pilot study confirmed the effectiveness of the current fin design. / Detta arbete fördjupar sig i design och estetik av en specifik produkt inom marinindustrin, en stabiliseringsfena. Arbetet sker i samarbete med företaget Humphree som utvecklar och säljer en av dessa fenor. Humphree’s allspeedfenor monteras på yachter för att stabilisera båten både under färd och stillastående. Fenorna ger användaren stabilitet och ökar komforten för hela besättningen samtidigt som de optimerar bränsleeffektiviteten. Eftersom denna fena utsätts för enorma belastningar och krafter bör fenan vara pålitlig samt förmedla en sådan känsla. Dessa kriterier uppfylls inte idag, och är därför en del av den första målsättningen för denna uppsats, “Hur kan designen uppdateras för att stämma överens med kundens förväntningar?”. Humphree’s fokus är att stabilisera marina fartyg med sina två huvudprodukter, stabiliseringsfenor och deras Interceptorer. Samtidigt som Humphree växer bör företaget utforska nya marknader inom den marina sektorn och skapa produkter med gemensamma designkaraktäristiska drag, vilket är en del av den andra målsättningen för denna uppsats, “Hur kan produkten bättre visa sina förmågor?”. Den marina marknaden ses redan som en bov till miljöpåverkan, särskilt inom den privata sektorn. Detta är något som skulle kunna, och bör, förbättras då en stor del av världens befolkning efterfrågar mer miljövänliga produkter. Detta undersöks i den sista målsättningen, “Hur kan den miljömässiga påverkan av produkten minskas?”. Under pilotstudien tillämpades principerna för användarcentrerad design för att förbättra forskningskvaliteten och underlätta identifieringen av samband mellan olika informationsinstanser. Huvudsyftet var att genomföra en pilotstudie för att identifiera framtida krav för stabiliseringsfenor. Detta innebar främst intervjuer med montörer och personer från Humphree, samt benchmarking och slutligen en designgranskning för att fastställa nödvändiga tekniska lösningar. För det andra skulle ett nytt koncept av fenan utformas enligt resultaten från pilotstudien. Arbetet resulterade i att det nya konceptet skulle ha ett materialbyte från kolfiber till aluminium. Aluminium förbättrar fenans robusthet och möjliggör reparation, vilket därmed förbättrar dess miljövänlighet. Designjusteringar av exteriören gjordes för att anpassa sig till Humphree’s produktdesign, optimera monteringsprocessen och förstärka fenans robusta utseende. Designändringarna var dock små eftersom pilotstudien bekräftade att den nuvarande fenans design var effektiv