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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
101

Automatic index generation for the free-text based database.

January 1992 (has links)
by Leung Chi Hong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 183-184). / Chapter Chapter one: --- Introduction --- p.1 / Chapter Chapter two: --- Background knowledge and linguistic approaches of automatic indexing --- p.5 / Chapter 2.1 --- Definition of index and indexing --- p.5 / Chapter 2.2 --- Indexing methods and problems --- p.7 / Chapter 2.3 --- Automatic indexing and human indexing --- p.8 / Chapter 2.4 --- Different approaches of automatic indexing --- p.10 / Chapter 2.5 --- Example of semantic approach --- p.11 / Chapter 2.6 --- Example of syntactic approach --- p.14 / Chapter 2.7 --- Comments on semantic and syntactic approaches --- p.18 / Chapter Chapter three: --- Rationale and methodology of automatic index generation --- p.19 / Chapter 3.1 --- Problems caused by natural language --- p.19 / Chapter 3.2 --- Usage of word frequencies --- p.20 / Chapter 3.3 --- Brief description of rationale --- p.24 / Chapter 3.4 --- Automatic index generation --- p.27 / Chapter 3.4.1 --- Training phase --- p.27 / Chapter 3.4.1.1 --- Selection of training documents --- p.28 / Chapter 3.4.1.2 --- Control and standardization of variants of words --- p.28 / Chapter 3.4.1.3 --- Calculation of associations between words and indexes --- p.30 / Chapter 3.4.1.4 --- Discarding false associations --- p.33 / Chapter 3.4.2 --- Indexing phase --- p.38 / Chapter 3.4.3 --- Example of automatic indexing --- p.41 / Chapter 3.5 --- Related researches --- p.44 / Chapter 3.6 --- Word diversity and its effect on automatic indexing --- p.46 / Chapter 3.7 --- Factors affecting performance of automatic indexing --- p.60 / Chapter 3.8 --- Application of semantic representation --- p.61 / Chapter 3.8.1 --- Problem of natural language --- p.61 / Chapter 3.8.2 --- Use of concept headings --- p.62 / Chapter 3.8.3 --- Example of using concept headings in automatic indexing --- p.65 / Chapter 3.8.4 --- Advantages of concept headings --- p.68 / Chapter 3.8.5 --- Disadvantages of concept headings --- p.69 / Chapter 3.9 --- Correctness prediction for proposed indexes --- p.78 / Chapter 3.9.1 --- Example of using index proposing rate --- p.80 / Chapter 3.10 --- Effect of subject matter on automatic indexing --- p.83 / Chapter 3.11 --- Comparison with other indexing methods --- p.85 / Chapter 3.12 --- Proposal for applying Chinese medical knowledge --- p.90 / Chapter Chapter four: --- Simulations of automatic index generation --- p.93 / Chapter 4.1 --- Training phase simulations --- p.93 / Chapter 4.1.1 --- Simulation of association calculation (word diversity uncontrolled) --- p.94 / Chapter 4.1.2 --- Simulation of association calculation (word diversity controlled) --- p.102 / Chapter 4.1.3 --- Simulation of discarding false associations --- p.107 / Chapter 4.2 --- Indexing phase simulation --- p.115 / Chapter 4.3 --- Simulation of using concept headings --- p.120 / Chapter 4.4 --- Simulation for testing performance of predicting index correctness --- p.125 / Chapter 4.5 --- Summary --- p.128 / Chapter Chapter five: --- Real case study in database of Chinese Medicinal Material Research Center --- p.130 / Chapter 5.1 --- Selection of real documents --- p.130 / Chapter 5.2 --- Case study one: Overall performance using real data --- p.132 / Chapter 5.2.1 --- Sample results of automatic indexing for real documents --- p.138 / Chapter 5.3 --- Case study two: Using multi-word terms --- p.148 / Chapter 5.4 --- Case study three: Using concept headings --- p.152 / Chapter 5.5 --- Case study four: Prediction of proposed index correctness --- p.156 / Chapter 5.6 --- Case study five: Use of (Σ ΔRij) Fi to determine false association --- p.159 / Chapter 5.7 --- Case study six: Effect of word diversity --- p.162 / Chapter 5.8 --- Summary --- p.166 / Chapter Chapter six: --- Conclusion --- p.168 / Appendix A: List of stopwords --- p.173 / Appendix B: Index terms used in case studies --- p.174 / References --- p.183
102

Innovative fuzzy Petri net model for Chinese medicine expert system.

January 2002 (has links)
by Leung Wing-shan, Queenie. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (leaves 79-82). / Abstracts in English and Chinese. / Abstract --- p.iii / Acknowledgements --- p.v / Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Current Problem --- p.4 / Chapter 1.2 --- Research Proposal --- p.6 / Chapter 1.3 --- Research Target --- p.8 / Chapter 1.4 --- Thesis Overview --- p.9 / Chapter 2 --- Fuzzy Logic and Fuzzy Petri Net --- p.11 / Chapter 2.1 --- Background --- p.13 / Chapter 2.2 --- Fuzzy sets --- p.15 / Chapter 2.3 --- Operations on fuzzy sets --- p.18 / Chapter 2.4 --- Fuzzy logic --- p.20 / Chapter 2.5 --- Weighted Fuzzy Petri Net --- p.23 / Chapter 2.6 --- Fuzzy reasoning --- p.25 / Chapter 2.7 --- More about fuzzy logic --- p.29 / Chapter 2.8 --- Chapter Summary --- p.31 / Chapter 3 --- Dynamic Certainty Factor --- p.32 / Chapter 3.1 --- Definition --- p.32 / Chapter 3.1.1 --- Background --- p.33 / Chapter 3.1.2 --- Examples --- p.37 / Chapter 3.2 --- Advantages --- p.43 / Chapter 3.2.1 --- Best reasoning --- p.43 / Chapter 3.2.2 --- Independency --- p.46 / Chapter 3.2.3 --- Interaction effect --- p.49 / Chapter 3.3 --- Chapter Summary --- p.51 / Chapter 4 --- Experiment --- p.53 / Chapter 4.1 --- Transformation Definition --- p.54 / Chapter 4.2 --- Case Study --- p.61 / Chapter 4.2.1 --- Example 1 --- p.61 / Chapter 4.2.2 --- Example 2 --- p.63 / Chapter 4.3 --- Analysis --- p.65 / Chapter 4.3.1 --- Comparisons --- p.65 / Chapter 4.3.2 --- Discussion --- p.67 / Chapter 4.4 --- Chapter Summary --- p.68 / Chapter 5 --- Conclusion --- p.69 / Chapter 5.1 --- Final Summary --- p.69 / Chapter 5.2 --- Deficiency and Improvement --- p.71 / Chapter 5.3 --- Future Research Aspect --- p.73 / Appendix --- p.75 / Chapter A --- Data Details --- p.76 / Bibliography --- p.79
103

Authentication by molecular method of dendrobium used in Chinese medicine.

January 2000 (has links)
by Lau Tai Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 117-127). / Abstracts in English and Chinese. / Table of Content --- p.i / Abbreviations --- p.iv / Abstract --- p.v / List of Figures --- p.ix / List of Tables --- p.xii / Chapter 1. --- Chapter One: Introduction --- p.1 / Chapter 1.1 --- Background on orchids --- p.2 / Chapter 1.2 --- Background on Dendrobium --- p.7 / Chapter 1.3 --- Background and history on Herba Dendrobii --- p.9 / Chapter 1.4 --- Reasons for study of Herba Dendrobii --- p.12 / Chapter 1.4.1 --- Demand --- p.12 / Chapter 1.4.2 --- Adulteration --- p.13 / Chapter 1.4.3 --- CITES --- p.13 / Chapter 1.5 --- Scientific researches on Herba Dendrobii --- p.14 / Chapter 1.5.1 --- Morphological studies --- p.15 / Chapter 1.5.2 --- Anatomical and microscopic studies --- p.16 / Chapter 1.5.3 --- Phytochemistry --- p.20 / Chapter 1.5.3.1 --- Chemicals identified --- p.20 / Chapter 1.5.3.2 --- Chemical authentication of Herba Dendrobii --- p.23 / Chapter 1.5.3.3 --- Effect of treatment on chemical composition --- p.23 / Chapter 1.5.4 --- Phylogenetic study of Dendrobium --- p.25 / Chapter 1.5.4.1 --- Phylogenetic analysis by molecular methods --- p.25 / Chapter 1.5.4.2 --- Phylogenetic analysis by anatomical methods --- p.27 / Chapter 1.5.5 --- Pharmacological effect --- p.29 / Chapter 2. --- Chapter two: Objectives and strategies --- p.30 / Chapter 3. --- Chapter Three: Materials and Methods --- p.33 / Chapter 3.1 --- Source of samples and their treatment --- p.34 / Chapter 3.1.1 --- Fresh materials --- p.34 / Chapter 3.1.2 --- Dry materials --- p.34 / Chapter 3.1.3 --- Outgroup species --- p.35 / Chapter 3.2 --- Experimental protocol --- p.40 / Chapter 3.2.1 --- Rationale of the experiment --- p.40 / Chapter 3.2.2 --- DNA extraction --- p.41 / Chapter 3.2.2.1 --- Cetyltrimethylammonium bromide extraction method --- p.41 / Chapter 3.2.2.1a --- Reagents and buffers --- p.41 / Chapter 3.2.2.1b --- Procedures of CTAB extraction method --- p.42 / Chapter 3.2.2.2 --- Modified DNA isolation protocol for dry samples --- p.43 / Chapter 3.2.2.2a --- Reagents and buffers --- p.43 / Chapter 3.2.2.2b --- Procedures of modified DNA isolation protocol for dry plant samples --- p.44 / Chapter 3.2.3 --- Agarose gel electrophoresis of genomic DNA or PCR products --- p.45 / Chapter 3.2.3a --- Reagents and buffers --- p.45 / Chapter 3.2.3b --- Procedures of agarose gel electrophoresis of genomic DNA or PCR products --- p.45 / Chapter 3.2.4 --- Qualitative and quantitative analysis of DNA --- p.46 / Chapter 3.2.5 --- Amplification of the internal transcribed spacer 2 (ITS 2) region by Polymerase Chain Reaction --- p.47 / Chapter 3.2.5a --- Internal transcribed spacer 2 (ITS 2) region --- p.47 / Chapter 3.2.5b --- Procedures of polymerase chain reaction of ITS 2 region --- p.48 / Chapter 3.2.6 --- Purification of PCR products or cycle sequencing products --- p.48 / Chapter 3.2.6.1 --- Ethanol precipitation --- p.48 / Chapter 3.2.6.2 --- GENECLEAN® protocols --- p.49 / Chapter 3.2.6.3 --- Spin Column Purification --- p.49 / Chapter 3.2.7 --- Cycle Sequencing --- p.50 / Chapter 3.2.8 --- Sample Electrophoresis --- p.51 / Chapter 3.2.8a --- Equipment and reagents --- p.51 / Chapter 3.2.8b --- Procedures of sample electrophoresis --- p.52 / Chapter 3.2.9 --- Sequence analysis --- p.52 / Chapter 4. --- Results --- p.53 / Chapter 4.1 --- Fresh materials --- p.54 / Chapter 4.1.1 --- Genomic DNA --- p.54 / Chapter 4.1.2 --- PCR products --- p.59 / Chapter 4.1.3 --- Sequence alignment --- p.66 / Chapter 4.1.4 --- Comparison of the sequences --- p.94 / Chapter 4.1.5 --- Percentage difference among Dendrobium --- p.96 / Chapter 4.1.6 --- Intra-specific variation of orchid species --- p.96 / Chapter 4.1.7 --- Phylogenetic analysis --- p.99 / Chapter 4.2 --- Dry materials --- p.101 / Chapter 4.2.1 --- Genomic DNA --- p.101 / Chapter 4.2.2 --- PCR products --- p.101 / Chapter 4.2.3 --- Sequencing result --- p.101 / Chapter 5. --- Discussion and Conclusion --- p.107 / Chapter 5.1 --- Reasons for authentication of Herba Dendrobii --- p.108 / Chapter 5.2 --- Fresh materials of Herba Dendrobii --- p.109 / Chapter 5.2.1 --- Authentication --- p.109 / Chapter 5.2.2 --- Phylogenetic analysis --- p.111 / Chapter 5.3 --- Dry materials of Herba Dendrobii --- p.114 / Chapter 5.4 --- Evaluation of the experimental method --- p.115 / Chapter 5.5 --- Conclusion --- p.116 / Chapter 6. --- Reference --- p.117 / Chapter 7. --- Appendix / Appendix 1: Number of species in each medicinal orchid geneus --- p.128 / Appendix 2: Photographs showing 15 of the 17 species of orchids used in this research project --- p.131
104

Antioxidative activity of aqueous extracts from the herbal components of the traditional Chinese medicinal formula Wu-zi-yan-zong-wan.

January 2002 (has links)
by Yau Ming Hon. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (leaves 131-154). / Abstracts in English and Chinese. / Contents --- p.i / Acknowledgements --- p.ix / Abstract --- p.x / 槪論 --- p.xi / List of abbreviations --- p.xii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Reactive oxygen species (ROS) --- p.2 / Chapter 1.1.1 --- Hydrogen peroxide --- p.2 / Chapter 1.1.2 --- Superoxide anion --- p.3 / Chapter 1.1.3 --- Hydroxyl radical --- p.3 / Chapter 1.1.4 --- Carbon centered radical --- p.4 / Chapter 1.1.5 --- Nitric oxide --- p.4 / Chapter 1.2 --- Physiological roles of ROS --- p.5 / Chapter 1.2.1 --- Signal transduction --- p.5 / Chapter 1.2.2 --- Phagocytic activity --- p.6 / Chapter 1.3 --- Defense systems against ROS --- p.7 / Chapter 1.3.1 --- Endogenous antioxidant enzymes --- p.8 / Chapter 1.3.1.1 --- Catalase --- p.8 / Chapter 1.3.1.2 --- Superoxide dismutase --- p.8 / Chapter 1.3.1.3 --- Selenium-glutathione peroxidase --- p.9 / Chapter 1.3.1.4 --- Glutathione reductase --- p.10 / Chapter 1.3.1.5 --- Glutathione-S-transferases --- p.10 / Chapter 1.3.2 --- Non-enzymatic antioxidants --- p.12 / Chapter 1.3.2.1 --- Vitamin E (tocopherols and tocotrienols) --- p.12 / Chapter 1.3.2.2 --- Vitamin C (L-ascorbic acid) --- p.13 / Chapter 1.3.2.3 --- Glutathione --- p.14 / Chapter 1.3.2.4 --- Flavonoids and polyphenols --- p.15 / Chapter 1.3.2.5 --- Uric acid --- p.16 / Chapter 1.4 --- Roles of ROS in pathogenesis --- p.16 / Chapter 1.4.1 --- Liver diseases --- p.17 / Chapter 1.4.2 --- Genital malfunctioning --- p.19 / Chapter 1.5 --- "The traditional Chinese medicinal formula, Wu-zi-yan-zong-wan" --- p.19 / Chapter 1.5.1 --- Pharmacology of individual herbal components --- p.20 / Chapter 1.5.1.1 --- Semen Cuscuta --- p.20 / Chapter 1.5.1.2 --- Fructus Lycii --- p.21 / Chapter 1.5.1.3 --- Fructus Schisandrae --- p.21 / Chapter 1.5.1.4 --- Fructus Rubi --- p.22 / Chapter 1.5.1.5 --- Semen Plantaginis --- p.22 / Chapter 1.5.2 --- Effect of Wu-zi-yan-zong-wan on infertility --- p.23 / Chapter 1.5.3 --- Effect of Wu-zi-yan-zong-wan on liver disease --- p.23 / Chapter 1.6 --- Objectives of the present study --- p.24 / Chapter Chapter 2 --- Antioxidant Activity of Aqueous Extracts of the Herbal Components of Wu-zi-yan-zong-wan in in vitro Free Radical Generating Systems --- p.26 / Chapter 2.1 --- Introduction --- p.27 / Chapter 2.1.1 --- Application of in vitro ROS generating systems --- p.27 / Chapter 2.1.1.1 --- Superoxide generation --- p.27 / Chapter 2.1.1.2 --- Hydroxyl radical generation system --- p.28 / Chapter 2.1.1.3 --- "2,2'-Azobis(2-amidinopropane) dihydrochloride- induced hemolysis" --- p.28 / Chapter 2.1.1.4 --- Bleomycin-iron-dependent DNA damage --- p.28 / Chapter 2.1.2 --- Objective --- p.29 / Chapter 2.2 --- Materials and methods --- p.30 / Chapter 2.2.1 --- Materials --- p.30 / Chapter 2.2.2 --- Preparation of aqueous herbal extracts --- p.30 / Chapter 2.2.3 --- Superoxide-scavenging assay --- p.30 / Chapter 2.2.4 --- Microsome lipid peroxidation inhibition assay --- p.31 / Chapter 2.2.5 --- "2,2'-Azobis(2-amidinopropane) dihydrochloride-induced hemolysis inhibition assay" --- p.32 / Chapter 2.2.6 --- Bleomycin-iron-dependent DNA damage inhibition assay --- p.32 / Chapter 2.2.7 --- Statistical analysis --- p.33 / Chapter 2.3 --- Results --- p.34 / Chapter 2.3.1 --- Extraction yield --- p.34 / Chapter 2.3.2 --- Free radical scavenging activity of Wu-zi-yan-zong-wan extract --- p.34 / Chapter 2.3.3 --- Free radical scavenging activity of FR extract --- p.37 / Chapter 2.3.3.1 --- Superoxide-scavenging activity --- p.37 / Chapter 2.3.3.2 --- Effect on hydroxyl radical-induced lipid peroxidation --- p.37 / Chapter 2.3.3.3 --- Effect on AAPH-induced hemolysis --- p.40 / Chapter 2.3.3.4 --- Effect on bleomycin-iron-dependent DNA damage --- p.40 / Chapter 2.3.4 --- Pro-oxidant activity of FR extract --- p.40 / Chapter 2.3.5 --- Free radical scavenging activity of the remaining herbal extracts --- p.44 / Chapter 2.4 --- Discussion --- p.46 / Chapter Chapter 3 --- Effect of Aqueous Extract of the Herbal Components of Wu- zi-yan-zong-wan on tert-Butyl Hydroperoxide-Induced Oxidative Damage in Primary Rat Hepatocyte --- p.51 / Chapter 3.1 --- Introduction --- p.52 / Chapter 3.1.1 --- Primary rat hepatocyte as pharmacological model --- p.52 / Chapter 3.1.2 --- tert-Butyl hydroperoxide as an oxidative stress inducer --- p.53 / Chapter 3.1.3 --- Detection of ROS --- p.54 / Chapter 3.1.4 --- Objective --- p.55 / Chapter 3.2 --- Materials and methods --- p.56 / Chapter 3.2.1 --- Materials --- p.56 / Chapter 3.2.2 --- Primary rat hepatocyte isolation --- p.56 / Chapter 3.2.2.1 --- Liver perfusion --- p.56 / Chapter 3.2.2.2 --- Collagen pre-coated plates preparation --- p.57 / Chapter 3.2.2.3 --- Hepatocyte culture --- p.58 / Chapter 3.2.3 --- Drug treatment and oxidative stress induction --- p.58 / Chapter 3.2.4 --- Cytotoxicity assessment --- p.58 / Chapter 3.2.4.1 --- Lactate dehydrogenase leakage measurement --- p.59 / Chapter 3.2.4.2 --- MTT assay --- p.59 / Chapter 3.2.5 --- Cellular GSH content determination --- p.59 / Chapter 3.2.6 --- Protein determination by Lowry's method --- p.60 / Chapter 3.2.7 --- MDA measurement --- p.60 / Chapter 3.2.8 --- GSSG measurement --- p.61 / Chapter 3.2.9 --- ROS measurement with fluorescent dye --- p.61 / Chapter 3.2.10 --- "Vitamin C, vitamin E and butylated hydroxytoluene treatment" --- p.62 / Chapter 3.2.11 --- Antioxidant enzyme activity measurement --- p.62 / Chapter 3.2.11.1 --- Catalase activity measurement --- p.62 / Chapter 3.2.11.2 --- Superoxide dismutase activity measurement --- p.63 / Chapter 3.2.11.3 --- Glutathione peroxidase activity measurement --- p.63 / Chapter 3.2.11.4 --- Glutathione-S-transferases activity measurement --- p.63 / Chapter 3.2.11.5 --- Glutathione reductase activity measurement --- p.64 / Chapter 3.2.12 --- Statistical analysis --- p.64 / Chapter 3.3 --- Results --- p.65 / Chapter 3.3.1 --- Cytotoxicity of FR extract on rat hepatocyte --- p.65 / Chapter 3.3.2 --- Effect of tBHP and FR extract on hepatocyte viability --- p.65 / Chapter 3.3.3 --- Time-dependent effect of FR extract on tBHP-induced cytotoxicity --- p.69 / Chapter 3.3.4 --- Effect of tBHP and FR extract on hepatocyte GSH content --- p.69 / Chapter 3.3.5 --- Effect of tBHP and FR extract on GSSG formation in hepatocyte --- p.72 / Chapter 3.3.6 --- Effect of tBHP and FR extract on MDA formation in hepatocyte --- p.72 / Chapter 3.3.7 --- ROS-scavenging activity of FR extract in hepatocyte --- p.77 / Chapter 3.3.8 --- Effect of FR extract on antioxidant enzymes activities --- p.77 / Chapter 3.3.9 --- Comparison between typical antioxidants --- p.77 / Chapter 3.3.10 --- Effect of WZ and remaining herbal extracts on tBHP-induced oxidative damage in hepatocyte --- p.81 / Chapter 3.4 --- Discussion --- p.84 / Chapter Chapter 4 --- Effect of Aqueous Extract of Wu-zi-yan-zong-wan and Fructus Rubi on tert-Buty Hydroperoxide Induced Oxidative Damage in Mouse Model --- p.91 / Chapter 4.1 --- Introduction --- p.92 / Chapter 4.2 --- Materials and methods --- p.93 / Chapter 4.2.1 --- Materials --- p.93 / Chapter 4.2.2 --- Animal treatments --- p.93 / Chapter 4.2.3 --- Serum preparation --- p.94 / Chapter 4.2.4 --- Marker enzyme measurement --- p.94 / Chapter 4.2.5 --- Liver MDA and GSH determination --- p.95 / Chapter 4.2.6 --- Statistical analysis --- p.95 / Chapter 4.3 --- Results --- p.97 / Chapter 4.3.1 --- Effect of tBHP and FR extract on mouse serum ALT and AST activities --- p.97 / Chapter 4.3.2 --- Effect of tBHP and FR extract on mouse liver MDA and GSH content --- p.97 / Chapter 4.3.3 --- Effect of WZ extract on tBHP-induced increase in serum ALT and AST activities --- p.97 / Chapter 4.4 --- Discussion --- p.102 / Chapter Chapter 5 --- Characterization of the Active Antioxidant Principlein Aqueous Extract of FR --- p.105 / Chapter 5.1 --- Introduction --- p.106 / Chapter 5.2 --- Materials and methods --- p.107 / Chapter 5.2.1 --- Materials --- p.107 / Chapter 5.2.2 --- Chemical/physical treatments on FR extract --- p.107 / Chapter 5.2.3 --- Digestion with enzymes --- p.108 / Chapter 5.2.4 --- Antioxidant activity determination --- p.109 / Chapter 5.2.5 --- Chemical composition determination --- p.109 / Chapter 5.2.5.1 --- Uronic acid determination --- p.109 / Chapter 5.2.5.2 --- Hexose determination --- p.109 / Chapter 5.2.5.3 --- Tannin determination --- p.110 / Chapter 5.2.5.4 --- Protein determination --- p.110 / Chapter 5.2.6 --- Column chromatography --- p.110 / Chapter 5.2.6.1 --- Polyamide CC6 resin column chromatography --- p.111 / Chapter 5.2.6.2 --- Sephadex LH-20 gel column chromatography --- p.111 / Chapter 5.2.7 --- Antioxidant activity of commercially available tannin --- p.111 / Chapter 5.2.8 --- Bovine serum albumin precipitation --- p.112 / Chapter 5.2.9 --- Statistical analysis --- p.112 / Chapter 5.3 --- Results --- p.113 / Chapter 5.3.1 --- Effect of chemical/physical treatments on antioxidant activity of FR extract --- p.113 / Chapter 5.3.2 --- Effect of enzyme digestions on antioxidant activity of FR extract --- p.113 / Chapter 5.3.3 --- Chemical composition of FR extract --- p.118 / Chapter 5.3.4 --- Polyamide CC6 resin column chromatography --- p.118 / Chapter 5.3.5 --- Sephadex LH-20 gel column chromatography --- p.118 / Chapter 5.3.6 --- Antioxidant activity of commercially available tannin --- p.123 / Chapter 5.3.7 --- Effect of BSA precipitation on superoxide-scavenging activity --- p.123 / Chapter 5.4 --- Discussion --- p.127 / Conclusion --- p.131 / References --- p.132
105

Interface of diagnosis of Chinese medicine and western medicine on chronic hepatitis B.

January 2006 (has links)
Law Man Yee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 123-132). / Abstracts in English and Chinese; appendix in Chinese. / Precis --- p.vi / 摘要 --- p.viii / Captions for Tables --- p.x / Caption for Figure --- p.xii / Abbreviations --- p.xiii / Chapter Part 1 --- Literature Review --- p.0 / Chapter Chapter 1 --- Aims and Hypothesis --- p.1 / Chapter 1.1 --- Aims --- p.3 / Chapter 1.2 --- Hypothesis --- p.4 / Chapter Chapter 2 --- Epidemiology of HBV Infection --- p.5 / Chapter 2.1 --- Global and Local Epidemiology --- p.6 / Chapter 2.2 --- Modes of Transmission --- p.8 / Chapter 2.2.1 --- Perinatal Tansmission --- p.8 / Chapter 2.2.2 --- Percutaneous Transmission --- p.8 / Chapter 2.2.3 --- Sexual Transmission --- p.8 / Chapter 2.2.4 --- Healthcare Setting and Transplantation --- p.8 / Chapter 2.2.5 --- Transfusion --- p.9 / Chapter 2.2.6 --- Other --- p.9 / Chapter Chapter 3 --- Popularity of TCM --- p.10 / Chapter 3.1 --- Traditional Chinese Medicine Use --- p.11 / Chapter 2.2 --- Regulation on TCM Practice --- p.12 / Chapter Chapter 4 --- Philosophy of TCM on HBV Infection --- p.13 / Chapter 4.1 --- Basic Principles --- p.14 / Chapter 4.1.1 --- Yin-Yang Theory --- p.14 / Chapter 4.1.2 --- Five Elements Theory --- p.14 / Chapter 4.1.3 --- Zang Fu Theory --- p.14 / Chapter 4.2 --- Pathogenesis --- p.16 / Chapter 4.3 --- Diagnosis --- p.17 / Chapter 4.4 --- Treatment --- p.20 / Chapter Chapter 5 --- Western Medicine --- p.23 / Chapter 5.1 --- Natural History of HBV Infection --- p.24 / Chapter 5.1.1 --- Replicative Phase: Immune Tolerance --- p.24 / Chapter 5.1.2 --- Replicative Phase: Immune Clearance --- p.24 / Chapter 5.1.3 --- Noreplicating (Low-Replication) Phase --- p.25 / Chapter 5.2 --- Diagnostic Tests for HBV Infection --- p.26 / Chapter 5.2.1 --- Serologic Assays --- p.26 / Chapter 5.2.2 --- Serum Enzymes --- p.26 / Chapter 5.2.3 --- HBV DNA Assays --- p.27 / Chapter Chapter 6 --- Health-related Quality of Life --- p.29 / Chapter 6.1 --- Principle and Definition --- p.30 / Chapter 6.2 --- Assessment --- p.31 / Chapter 6.2.1 --- Generic Instrument --- p.31 / Chapter 6.2.2 --- Disease-Specific Instrument --- p.32 / Chapter Part 2 --- Studies & Results --- p.33 / Chapter Chapter 7 --- Research Methodology --- p.34 / Chapter 7.1 --- Study 1: Hospital-Based Surveys on Chronic Hepatitis B Patients: TCM Use --- p.35 / Chapter 7.1.1 --- Patients --- p.35 / Chapter 7.1.2 --- Survey Instrument & Logistic --- p.35 / Chapter 7.1.4 --- Statistical Analysis --- p.36 / Chapter 7.1.4 --- Sample Size Justification --- p.36 / Chapter 7.2 --- Study 2: Hospital-Based Survey on Chronic Hepatitis B Patients: HRQoL & Psychiatric Involvement --- p.38 / Chapter 7.2.1 --- Patients --- p.38 / Chapter 7.2.2 --- Survey Instrument and Logistic --- p.38 / Chapter 7.2.3 --- Statistical Analysis --- p.39 / Chapter 7.3 --- Study 3: Population-Based Survey on Chinese Medicine Practitioners (CMPs): Practice Behavior & Knowledge Assessment --- p.41 / Chapter 7.3.1 --- Study Population --- p.41 / Chapter 7.3.2 --- Survey Instrument --- p.41 / Chapter 7.3.3 --- Statistical Analysis & Knowledge Assessment --- p.42 / Chapter 7.4 --- Study 4: TCM Consultation Agreement --- p.44 / Chapter 7.4.1 --- Patients --- p.44 / Chapter 7.4.2 --- Chinese Medicine Practitioners --- p.44 / Chapter 7.4.3 --- Questionnaire --- p.44 / Chapter 7.4.4 --- Study Design --- p.45 / Chapter 7.4.5 --- Sample Size Estimation --- p.45 / Chapter 7.4.6 --- Data Analysis --- p.45 / Chapter 7.5 --- "Study 5: TCM Interpretation of Laboratory, Imaging & HRQoL Assessment" --- p.47 / Chapter 7.5.1 --- Patients --- p.47 / Chapter 7.5.2 --- HRQoL Assessment --- p.47 / Chapter 7.5.3 --- Statistical Analysis --- p.47 / Chapter Chapter 8 --- TCM Use on Chronic Hepatitis B Patients --- p.48 / Chapter 8.1 --- Patient Characteristics --- p.49 / Chapter 8.2 --- Health-Seeking Behavior of TCM Users --- p.51 / Chapter 8.3 --- Determinants of TCM Use --- p.53 / Chapter 8.4 --- Common Herbal Ingredients Used --- p.56 / Chapter Chapter 9 --- Impacts of HBV Infection on Patients --- p.58 / Chapter 9.1 --- Patient Socio-Demographic and Clinical Characteristics --- p.59 / Chapter 9.2 --- Patient Symptoms and Anxiety /Depression --- p.61 / Chapter 9.3 --- HRQoL & its Determinants --- p.63 / Chapter 9.3.1 --- Physical Aspect of HRQoL --- p.63 / Chapter 9.3.2 --- Mental Aspect of HRQoL --- p.67 / Chapter Chapter 10 --- Chinese Medicine Practitioners' (CMP) Practice --- p.71 / Chapter 10.1 --- CMPs Demographics and Training --- p.72 / Chapter 10.2 --- Practice Behavior --- p.75 / Chapter 10.3 --- Diagnostic and Therapeutic Approach --- p.76 / Chapter 10.4 --- Professional Knowledge Assessment --- p.79 / Chapter 10.5 --- Determinants of Diagnostic Approach --- p.82 / Chapter Chapter 11 --- Agreement of TCM Diagnosis among Different CMPs --- p.85 / Chapter 11.1 --- Patients Characteristics & Disease Severity --- p.86 / Chapter 11.2 --- Agreement of TCM Diagnosis & Treatment --- p.88 / Chapter Chapter 12 --- Interpretation of TCM diagnosis --- p.93 / Chapter 12.1 --- Patients --- p.94 / Chapter 12.2 --- Clinical Characteristics --- p.96 / Chapter 12.3 --- HRQoL Assessment --- p.98 / Chapter Part 3 --- Discussion & Conclusions --- p.100 / Chapter Chapter 13 --- Discussion --- p.101 / Chapter 13.1 --- TCM Popularity and its Practice --- p.102 / Chapter 13.2 --- Impact of TCM Symptomatology & Anxiety /Depression on HRQoL --- p.107 / Chapter 13.3 --- TCM Diagnosis Consistency --- p.111 / Chapter 13.4 --- Association of TCM Diagnosis with Western Medicine --- p.114 / Chapter 13.5 --- Limitations of the Study --- p.117 / Chapter Chapter 14 --- Conclusions --- p.120 / Reference --- p.122 / List of Publications of My Work Used in This Thesis --- p.133 / Acknowledgement --- p.136 / Appendix --- p.138
106

Bioassay-guided isolation, characterization and mechanistic study of the bioactive components from Sophora flavescens for the anti-proliferative effect on human hepatoma cells.

January 2006 (has links)
by Tsang Kit Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 179-188). / Abstracts in English and Chinese. / ABSTRACT --- p.i / ABSTRACT IN CHINESE (摘要) --- p.iii / ACKNOWLEDGEMENTS --- p.v / CONTENTS --- p.vi / LIST OF FIGURES --- p.xi / LIST OF TABLES --- p.xiv / ABBREVIATIONS --- p.xvi / Chapter CHAPTER ONE: --- INTRODUCTION --- p.1 / Chapter 1.1 --- Hepatocellular Carcinoma --- p.2 / Chapter 1.1.1 --- Incidence of Hepatocellular Carcinoma --- p.2 / Chapter 1.1.2 --- Therapies for Hepatocellular Carcinoma --- p.4 / Chapter 1.2 --- Multidrug Resistance of Tumor Cells --- p.8 / Chapter 1.3 --- Therapeutic Potential of Traditional Chinese Medicine on Human Hepatoma --- p.10 / Chapter 1.4 --- Sophora flavescens Ait --- p.13 / Chapter 1.5 --- Biological Activities of Sophorae Radix --- p.15 / Chapter 1.5.1 --- Antitumor Activities --- p.16 / Chapter 1.5.2 --- "Antibacterial, Antimalarial and Antiviral Activities" --- p.17 / Chapter 1.6 --- Objectives and Significance of Study --- p.19 / Chapter 1.6.1 --- Bioassay-guided Isolation of Active Compounds from Sophora flavescens --- p.19 / Chapter 1.6.2 --- Action Mechanisms of the Bioactive Compounds Isolated from Sophora flavescens --- p.20 / Chapter CHAPTER TWO: --- MATERIALS AND METHODS --- p.21 / Chapter 2.1 --- Cell Culture --- p.22 / Chapter 2.1.1 --- Cell Lines --- p.22 / Chapter 2.1.2 --- Cell Culture Media --- p.24 / Chapter 2.2 --- Isolation of Bioactive Compounds from Sophora flavescens --- p.25 / Chapter 2.3 --- MTT assay --- p.27 / Chapter 2.4 --- Cell Cycle Analysis --- p.28 / Chapter 2.5 --- Detection of Phosphatidylserine Externalization with Annexin V-FITC and PI --- p.29 / Chapter 2.6 --- DNA Fragmentation Assay --- p.30 / Chapter 2.7 --- Western Blot Analysis --- p.32 / Chapter 2.7.1 --- Extraction of Total Cellular Protein --- p.32 / Chapter 2.7.2 --- Extraction of Cytosolic Protein --- p.32 / Chapter 2.7.3 --- Determination of Protein Concentration --- p.33 / Chapter 2.7.4 --- Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) --- p.35 / Chapter 2.7.5 --- Electroblotting of Protein --- p.36 / Chapter 2.7.6 --- Probing of Proteins with Antibodies --- p.37 / Chapter 2.7.7 --- Enhanced Chemiluminescence (ECL) Assay --- p.39 / Chapter 2.8 --- Detection of Mitochondrial Membrane Potential by JC-1 Fluorescent dye --- p.39 / Chapter 2.9 --- cDNA Microarray Analysis --- p.40 / Chapter 2.9.1 --- Isolation of Total RNA --- p.40 / Chapter 2.9.2 --- Microarray Hybridization and Analysis --- p.41 / Chapter 2.9.3 --- Validation of Candidate Genes --- p.44 / Chapter 2.9.3.1 --- Determination of RNA Concentration --- p.44 / Chapter 2.9.3.2 --- First-Strand cDNA Synthesis --- p.44 / Chapter 2.9.3.3 --- Reverse-Transcription Polymerase Chain Reaction (RT-PCR) of Candidate Genes --- p.45 / Chapter 2.10 --- Two-Dimensional Polyacrylamide Gel Electrophoretic Analysis (2D-PAGE) --- p.47 / Chapter 2.10.1 --- Extraction of Total Cellular Protein for 2-D Gel Electrophoresis --- p.47 / Chapter 2.10.2 --- Determination of Protein Concentration --- p.47 / Chapter 2.10.3 --- First-Dimension Isoelectric Focusing (IEF) --- p.49 / Chapter 2.10.4 --- Second-Dimension SDS-PAGE --- p.49 / Chapter 2.10.5 --- Visualization of 2-D Gel by Silver Staining --- p.50 / Chapter 2.10.6 --- Identification of Differentially Expressed Proteins with Matrix Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) --- p.51 / Chapter 2.11 --- Statistical Analysis --- p.53 / Chapter CHAPTER THREE: --- BIOASSAY-GUIDED ISOLATION AND CHARACTERISATION OF BIOACTIVE COMPOUNDS FROM SOPHORA FLAVESCENS --- p.54 / Chapter 3.1 --- Bioassay-guided Isolation of Bioactive Compounds from Sophora flavescens --- p.55 / Chapter 3.2 --- Structure Identification of the Bioactive Compounds Isolated from Sophora flavescens --- p.64 / Chapter 3.3 --- In Vitro Anti-tumor Effect of the Bioactive Compounds Isolated from Sophora flavescens --- p.71 / Chapter CHAPTER FOUR: --- MECHANISTIC STUDY OF SOPHORAFLAVANONE G IN THE INDUCTION OF APOPTOSIS IN HEPATOCELLULAR CARCINOMA CELLS --- p.76 / Chapter 4.1 --- In Vitro Anti-tumor Effect of Sophoraflavanone G --- p.77 / Chapter 4.2 --- Cell Cycle Analysis of Human Hepatocellular Carcinoma Cells and Multidrug Human Hepatocellular Carcinoma Cells --- p.81 / Chapter 4.3 --- Induction of Apoptosis in Hepatocellular Carcinoma Cells by Sophoraflavanone G --- p.88 / Chapter 4.3.1 --- Induction of Phosphatidylserine Externalization in Hepatocellular Carcinoma Cells by Sophoraflavanone G --- p.89 / Chapter 4.3.2 --- Induction of DNA Fragmentation in Hepatocellular Carcinoma Cells by Sophoraflavanone G --- p.94 / Chapter 4.3.3 --- Induction of Caspase-3 activation in Hepatocellular Carcinoma Cells by Sophoraflavanone G --- p.97 / Chapter 4.4 --- Underlying Mechanisms of Sophoraflavanone G-induced Apoptosis in Human Hepatocellular Carcinoma Cells --- p.102 / Chapter 4.4.1 --- Involvement of Death Receptor Pathway in Sophoraflavanone G- induced Apoptosis in Human Hepatocellular Carcinoma Cells --- p.103 / Chapter 4.4.2 --- Involvement of Bid protein in Sophoraflavanone G-induced Apoptosis in Human Hepatocellular Carcinoma Cells --- p.105 / Chapter 4.4.3 --- Involvement of Mitochondrial Pathway in Sophoraflavanone G- induced Apoptosis in Human Hepatocellular Carcinoma Cells --- p.108 / Chapter 4.4.4 --- Induction of Mitochondrial Membrane Depolarization in Human Hepatocellular Carcinoma Cells by Sophoraflavanone G --- p.112 / Chapter 4.4.5 --- Involvement of Caspase-independent Pathway in Sophoraflavanone G-induced Apoptosis in Human Hepatocellular Carcinoma Cells --- p.116 / Chapter CHAPTER FIVE: --- MECHANISTIC STUDY OF SOPHORAFLAVANONE G ON HUMAN HEPATOCELLULAR CARCINOMA CELLS BY USING cDNA MICROARRAY ANALYSIS --- p.119 / Chapter 5.1 --- Identification of Differentially Expressed Genes in Sophoraflavanone G- treated Human Hepatocellular Carcinoma Cells by cDNA Microarray Analyasis --- p.120 / Chapter CHAPTER SIX: --- MECHANISTIC STUDY OF SOPHORAFLAVANONE G ON HEPATOCELLULAR CARCINOMA CELLS BY USING TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS --- p.136 / Chapter 6.1 --- Identification of Differentially Expressed Proteins in Sophoraflavanone G- treated Human Hepatocellular Carcinoma Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis --- p.137 / Chapter CHAPTER SEVEN: --- DISCUSSION --- p.150 / Chapter 7.1 --- Bioassay-guided Isolation of Bioactive Compounds from Sophora flavescens --- p.151 / Chapter 7.2 --- Induction of Apoptosis in Human Hepatocellular Carcinoma cells and Multidrug Human Hepatocellular Carcinoma Cells --- p.154 / Chapter 7.3 --- Differential Gene Expression Induced by Sophoraflavanone G in Human Hepatocellular Carcinoma Cells --- p.161 / Chapter 7.4 --- Differential Protein Expression Induced by Sophoraflavanone G in Human Hepatocellular Carcinoma Cells and Multidrug Human Hepatocellular Carcinoma Cells --- p.164 / Chapter 7.5 --- Toxicity of Sophoraflavanone G against Normal Liver Cells --- p.170 / Chapter CHAPTER EIGHT: --- CONCLUSION AND FUTURE PERSPECTIVES --- p.173 / Chapter 8.1 --- Conclusion --- p.174 / Chapter 8.2 --- Future Prospects --- p.176 / REFERENCES --- p.179
107

Investigation of pharmacological anti-diabetic effect on selected traditional Chinese herbs.

January 2005 (has links)
by Lam Fung Chun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 187-202). / Abstracts in English and Chinese. / Abstract --- p.i / Abstract in Chinese --- p.iii / Acknowledgements --- p.v / Table of Contents --- p.vi / List of Abbreviations --- p.xiii / List of Tables --- p.xvii / List of Figures --- p.xviii / Publication --- p.xx / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Epidemiology of Diabetes Mellitus --- p.1 / Chapter 1.2 --- Definition of Diabetes Mellitus --- p.1 / Chapter 1.3 --- Glucose Homeostasis and Diabetes Mellitus --- p.2 / Chapter 1.4 --- Classification of Diabetes Mellitus --- p.6 / Chapter 1.4.1 --- Type 1 Diabetes Mellitus --- p.6 / Chapter 1.4.2 --- Type 2 Diabetes Mellitus --- p.7 / Chapter 1.4.3 --- Gestational Diabetes Mellitus --- p.8 / Chapter 1.4.4 --- Other specific types --- p.8 / Chapter 1.5 --- Diagnostic Criteria of Diabetes Mellitus --- p.9 / Chapter 1.6 --- Complications of Diabetes Mellitus --- p.11 / Chapter 1.7 --- Pharmacological Treatment of Diabetes --- p.12 / Chapter 1.7.1 --- Treatment for type 1 diabetes mellitus --- p.12 / Chapter 1.7.2 --- Treatment for Type 2 diabetes mellitus --- p.13 / Chapter 1.7.2.1 --- Sulfonylureas --- p.14 / Chapter 1.7.1.2 --- Meglitinides --- p.15 / Chapter 1.7.1.3 --- Biguanides --- p.15 / Chapter 1.7.1.4 --- Thazolidinediones --- p.16 / Chapter 1.7.1.5 --- α-Glucosidase inhibitor --- p.16 / Chapter 1.8 --- Diabetes and Traditional Chinese Medicine --- p.17 / Chapter 1.9 --- Objective of this project --- p.18 / Chapter Chapter 2 --- "Botanical, Preparation and Authentication of Traditional Chinese Herbs" --- p.22 / Chapter 2.1 --- Introduction --- p.22 / Chapter 2.2 --- Herbal Materials --- p.22 / Chapter 2.3 --- Authentication of Herbal Material --- p.30 / Chapter 2.4 --- Extraction Method --- p.32 / Chapter 2.4.1 --- Material and Methods --- p.32 / Chapter 2.4.2 --- Results --- p.32 / Chapter 2.4 --- Discussion --- p.32 / Chapter Chapter 3 --- In vitro Studies on Selected Traditional Chinese Herbs --- p.35 / Chapter 3.1. --- Introduction --- p.35 / Chapter 3.2 --- Hepatic Gluconeogenesis Studies --- p.36 / Chapter 3.2.1 --- Introduction --- p.36 / Chapter 3.2.2 --- Material and Methods --- p.41 / Chapter 3.2.2.1 --- Cell Culture of H4IIE --- p.41 / Chapter 3.2.2.2 --- Glucose Production Assay --- p.42 / Chapter 3.2.2.3 --- Bicinchoninic Acid (BCA) Protein Assay --- p.43 / Chapter 3.2.3 --- Results --- p.44 / Chapter 3.3 --- Intestinal Glucose Absorption Studies --- p.46 / Chapter 3.3.1 --- Introduction --- p.46 / Chapter 3.3.2 --- Material and Methods --- p.48 / Chapter 3.3.2.1 --- Preparation of BBMV --- p.48 / Chapter 3.3.2.1.1 --- Chemicals --- p.48 / Chapter 3.3.2.1.2 --- Method --- p.48 / Chapter 3.3.2.2 --- Preparation of Herbal Extracts --- p.50 / Chapter 3.3.2.3 --- BBMV Glucose Uptake Assay --- p.51 / Chapter 3.3.2.4 --- Bicinchoninic Acid (BCA) Protein Assay --- p.54 / Chapter 3.3.3 --- Results --- p.54 / Chapter 3.4 --- Fibroblast Glucose Uptake Studies --- p.57 / Chapter 3.4.1 --- Introduction --- p.57 / Chapter 3.4.2 --- Material and Methods --- p.58 / Chapter 3.4.2.1 --- Cell Culture of Hs68 --- p.58 / Chapter 3.4.2.2 --- 2-Deoxy-D-glucose Uptake Assay --- p.59 / Chapter 3.4.2.3 --- Bicinchoninic Acid (BCA) Protein Assay --- p.60 / Chapter 3.4.3 --- Results --- p.60 / Chapter 3.5 --- Adipocyte Glucose Uptake Studies --- p.63 / Chapter 3.5.1 --- Introduction --- p.63 / Chapter 3.5.2 --- Material and Methods --- p.65 / Chapter 3.5.2.1 --- Cell Culture of 3T3-L1 --- p.65 / Chapter 3.5.2.2 --- Differentiation of 3T3-L1 --- p.65 / Chapter 3.5.2.3 --- 2-Deoxy-D-glucose Uptake Assay --- p.66 / Chapter 3.5.2.4 --- Bicinchoninic Acid (BCA) Protein Assay --- p.68 / Chapter 3.5.3 --- Results --- p.69 / Chapter 3.6 --- Glucose Transporter Type 4 (GLUT4) Expression Studies --- p.71 / Chapter 3.6.1 --- Introduction --- p.71 / Chapter 3.6.2 --- Material and Methods --- p.48 / Chapter 3.6.2.1 --- Cell Culture of 3T3-L1 --- p.71 / Chapter 3.6.2.2 --- Differentiation of 3T3-L1 --- p.71 / Chapter 3.6.2.3 --- GLUT4 Expression Assay --- p.72 / Chapter 3.6.2.4 --- Preparation of RNA --- p.72 / Chapter 3.6.2.5 --- RT-PCR --- p.73 / Chapter 3.6.2.6 --- PCR Analysis on GLUT4 Expression --- p.74 / Chapter 3.6.2.7 --- Real-time PCR --- p.75 / Chapter 3.6.3 --- Results --- p.77 / Chapter 3.7 --- Discussion --- p.81 / Chapter 3.7.1 --- Discussion of Hepatic Gluconeogenesis Studies --- p.81 / Chapter 3.7.2 --- Discussion of Intestinal Glucose Absorption Studies --- p.82 / Chapter 3.7.3 --- Discussion of Fibroblast Glucose Uptake Studies --- p.83 / Chapter 3.7.4 --- Discussion of Adipocyte Glucose Uptake Studies --- p.84 / Chapter 3.7.5 --- Discussion of Glucose Transporter Type 4 (GLUT4) Expression Studies --- p.86 / Chapter 3.7.6 --- Conclusion --- p.87 / Chapter Chapter 4 --- Purification of Cortex Moutan --- p.90 / Chapter 4.1 --- Introduction --- p.90 / Chapter 4.1.1 --- Phytochemical Studies of Cortex Moutan --- p.90 / Chapter 4.2 --- Organic Extraction of Cortex Moutan --- p.93 / Chapter 4.2.1 --- Extraction Material and Methods --- p.93 / Chapter 4.2.2. --- Results --- p.93 / Chapter 4.3 --- BBMV Glucose Uptake Assay with Cortex Moutan Organic Extract (CM-C and CM-D) --- p.96 / Chapter 4.3.1 --- Material and Methods --- p.48 / Chapter 4.3.2 --- Results --- p.96 / Chapter 4.4 --- Fractionation of CM-C and CM-D --- p.98 / Chapter 4.4.1 --- Material and Methods --- p.98 / Chapter 4.4.1.1 --- Chemicals --- p.98 / Chapter 4.4.1.2 --- Methods --- p.98 / Chapter 4.4.2 --- Results --- p.100 / Chapter 4.5 --- BBMV Glucose Uptake Assay of CM-C and CM-D Sub-fractions --- p.105 / Chapter 4.5.1 --- Results --- p.105 / Chapter 4.6 --- Sulfonylation of CM-D1 --- p.107 / Chapter 4.6.1 --- Material and Methods --- p.107 / Chapter 4.6.1.1 --- Chemicals --- p.107 / Chapter 4.6.1.2 --- Methods --- p.107 / Chapter 4.6.2 --- Structure Elucidation of CM-D1s --- p.108 / Chapter 4.6.2.1 --- 1H-NMR Analysis --- p.108 / Chapter 4.6.3 --- BBMV Glucose Uptake Assay of CM-D1s --- p.108 / Chapter 4.6.4 --- Results --- p.108 / Chapter 4.7 --- "Structural Elucidation of CM-D3, CM-D4 and CM-D5" --- p.112 / Chapter 4.7.1 --- Material and Methods --- p.112 / Chapter 4.7.1.1 --- Mass Spectrometry --- p.112 / Chapter 4.7.1.2 --- 1H-NMR Analysis --- p.112 / Chapter 4.7.2 --- Results --- p.113 / Chapter 4.8 --- "BBMV Glucose Uptake Assay of Acetovallione, CM-D3,CM-D4 and CM-D5" --- p.116 / Chapter 4.8.1 --- Results --- p.116 / Chapter 4.9 --- Synthesis of CM-D3s --- p.118 / Chapter 4.9.1 --- Material and Methods --- p.118 / Chapter 4.9.1.1 --- Chemicals --- p.118 / Chapter 4.9.1.2 --- Methods --- p.118 / Chapter 4.9.2 --- Structure Elucidation of synthesized product --- p.119 / Chapter 4.9.3 --- Results --- p.119 / Chapter 4.10 --- Discussion --- p.121 / Chapter Chapter 5 --- In vivo Studies on Selected Herbs --- p.123 / Chapter 5.1 --- Introduction --- p.123 / Chapter 5.1.1 --- Diabetic Animal Models --- p.123 / Chapter 5.1.2 --- Neonatal Streptozotocin-induced Diabetic Rat Model --- p.125 / Chapter 5.2 --- Oral Glucose Tolerance Test (OGTT) --- p.126 / Chapter 5.2.1 --- Animal --- p.126 / Chapter 5.2.2 --- Rat Induction Material and Methods --- p.126 / Chapter 5.2.3 --- Testing Method for diabetic condition of rats --- p.127 / Chapter 5.3.4 --- Results --- p.128 / Chapter 5.3 --- Basal Glycaemia Test --- p.138 / Chapter 5.3.1 --- Animal --- p.138 / Chapter 5.3.2 --- Rat Induction Material and Methods --- p.138 / Chapter 5.3.3 --- Testing Method --- p.138 / Chapter 5.3.4 --- Results --- p.140 / Chapter 5.4 --- Discussion --- p.143 / Chapter Chapter 6 --- General Discussion --- p.147 / Chapter 6.1 --- Introduction --- p.147 / Chapter 6.2 --- Summary of Research Findings --- p.151 / Chapter 6.3 --- Result Interpretation --- p.152 / Chapter 6.3.1 --- Result Interpretation of In Vitro Studies --- p.152 / Chapter 6.3.2 --- Result Interpretation of Cortex Moutan Purification --- p.154 / Chapter 6.3.3 --- Result Interpretation of In Vivo Studies --- p.157 / Chapter 6.4 --- Limitations and Improvements --- p.161 / Chapter 6.5 --- Future Directions --- p.163 / Chapter 6.6 --- Conclusions --- p.169 / Appendices --- p.170 / References --- p.187
108

Molecular authentication of leigongteng and molecular cladistics of the subfamily tripterygioideae in celastraceae.

January 2006 (has links)
Law Ka Yee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 214-225). / Abstracts in English and Chinese. / ACKNOWLEDGEMENTS --- p.I / ABSTRACT --- p.II / TABLE OF CONTENTS --- p.VII / LIST OF FIGURES --- p.X / LIST OF TABLES A --- p.XII / APPENDIX --- p.XIII / Chapter CHAPTER ONE --- LITERATURE REVIEW --- p.1 / Chapter 1.1 --- Chinese herbs --- p.1 / Chapter 1.1.1 --- Introduction --- p.1 / Chapter 1.1.2 --- Leigongteng --- p.2 / Chapter 1.1.2.1 --- Leigongteng and its importance --- p.2 / Chapter 1.1.2.2 --- Chemical components and pharmacological effects of Leigongteng --- p.4 / Chapter 1.1.3 --- Problems in authentication of Leigongteng --- p.5 / Chapter 1.1.3.1 --- Taxonomic problems of Tripterygium --- p.5 / Chapter 1.1.3.2 --- Confusion caused by other species --- p.7 / Chapter 1.1..3.3 --- Adulterants --- p.9 / Chapter 1.2 --- Celastraceae --- p.10 / Chapter 1.2.1 --- Introduction --- p.10 / Chapter 1.2.2 --- Controversial taxonomic issue --- p.12 / Chapter 1.2.1.1 --- Subfamilies of Celastraceae --- p.12 / Chapter 1.2.1.2 --- Subfamily Tripterygioideae --- p.13 / Chapter 1.3 --- Molecular authentication --- p.14 / Chapter 1.4 --- Molecular systematics --- p.18 / Chapter 1.4.1 --- DNA sequence markers --- p.19 / Chapter 1.4.2 --- Molecular phylogeny --- p.25 / Chapter 1.4.2.1 --- Tree-building method --- p.25 / Chapter 1.4.2.2. --- Measures of support --- p.28 / Chapter 1.5 --- Objectives --- p.29 / Chapter CHAPTER TWO --- MATERIALS AND METHODS --- p.31 / Chapter 2.1 --- Plant and herb samples --- p.31 / Chapter 2.2 --- DNA extraction --- p.41 / Chapter 2.2.1 --- Modified CTAB extraction --- p.41 / Chapter 2.2.2 --- Commercial kit extraction --- p.42 / Chapter 2.3 --- Polymerase chain reaction (PCR) condition --- p.43 / Chapter 2.4 --- DNA gel electrophoresis --- p.44 / Chapter 2.5 --- PCR product purification --- p.45 / Chapter 2.5.1 --- GEL-M´ёØ gel extraction system --- p.45 / Chapter 2.6 --- Ligation and transformation --- p.46 / Chapter 2.6.1 --- Ligation and transformation --- p.46 / Chapter 2.6.2 --- Cell cultivation --- p.47 / Chapter 2.6.3 --- Plasmid extraction --- p.47 / Chapter 2.7 --- Determination of DNA concentration --- p.49 / Chapter 2.8 --- Cycle sequencing --- p.49 / Chapter 2.9 --- Sequence analysis --- p.50 / Chapter 2.10 --- Materials preparation --- p.51 / Chapter CHAPTER THREE --- MOLECULAR AUTHENTICATION OF LEIGONGTENG --- p.54 / Chapter 3.1. --- Authentication based on internal transcribed spacer (ITS) region --- p.54 / Chapter 3.1.1 --- Sequence alignment --- p.54 / Chapter 3.1.2 --- ITS region nucleotide differences significant in authentication of Leigongteng --- p.55 / Chapter 3.1.3 --- Relationship of samples --- p.70 / Chapter 3.1.4 --- Comparison of sequences --- p.75 / Chapter 3.2 --- Authentication based on 5s-rDNA region --- p.78 / Chapter 3.2.1 --- Sequence alignment --- p.78 / Chapter 3.2.2 --- 5s-rDNA nucleotide differences significant in authentication of Leigongteng --- p.78 / Chapter 3.2.3 --- Relationship of samples --- p.88 / Chapter 3.2.4 --- Comparison of sequences --- p.90 / Chapter 3.3 --- Authentication based on psbA-trnH region --- p.93 / Chapter 3.3.1 --- Sequence alignment --- p.93 / Chapter 3.3.2 --- psbA-trnH nucleotide differences significant in authentication of Leigongteng --- p.101 / Chapter 3.3.3 --- Relationship of samples --- p.113 / Chapter 3.3.4 --- Comparison of sequences --- p.115 / Chapter 3.4 --- Authentication based on trnL-F region --- p.118 / Chapter 3.4.1 --- Sequence alignment --- p.118 / Chapter 3.4.2 --- trnL-F region nucleotide differences significant in authentication of Leigongteng --- p.121 / Chapter 3.4.3 --- Relationship of samples --- p.139 / Chapter 3.4.4 --- Comparison of sequences --- p.141 / Chapter 3.5 --- Discussion --- p.144 / Chapter 3.5.1 --- Molecular markers --- p.144 / Chapter CHAPTER FOUR --- PHYLOGENETIC STUDIES ON TRIPTERYGIUM --- p.151 / Chapter 4.1 --- Combine loci of ITS and 5s-rDNA regions --- p.152 / Chapter 4.1.1 --- Homogenity test --- p.152 / Chapter 4.1.2 --- Sequence alignment --- p.152 / Chapter 4.1.3 --- Phylogenetic study --- p.173 / Chapter 4.2 --- psbA-trnH region --- p.174 / Chapter 4.2.1 --- Sequence alignment --- p.174 / Chapter 4.3 --- Discussion --- p.177 / Chapter CHAPTER FIVE --- PHYLOGENETIC STUDIES ON TRIPTERYGIOIDEAE AND CELASTRACEAE --- p.191 / Chapter 5.1 --- ITS regions --- p.191 / Chapter 5.1.1 --- Sequence alignment --- p.191 / Chapter 5.1.2 --- Phylogenetic analysis --- p.205 / Chapter 5.2 --- Discussion --- p.206 / Chapter 5.2.1 --- Subfamily Tripterygioideae --- p.206 / Chapter 5.2.2 --- Subfamilies of Celastraceae --- p.210 / Chapter CHAPTER SIX --- CONCLUSION --- p.212 / BILBIOGRAPHY --- p.214
109

Identifying Chinese medicinal materials with antinociceptive activities using a drosophila model /cChan, Kam Leung. / 應用果蠅模型進行鎮痛中藥篩選研究 / CUHK electronic theses & dissertations collection / Ying yong guo ying mo xing jin xing zhen tong zhong yao shai xuan yan jiu

January 2007 (has links)
An alternative complementary approach was used to verify the antinociceptive effect of 4 CMMs aqueous extracts in a Drosophila adult model. Drosophila adults were subjected to CMM treatments and then placed on an in-house-designed heating device for noxious heat stimulation. Their behavioral outputs were quantified and expressed as heat avoidance index (AI) for revealing the degree of antinociceptive effect of CMMs. By comparing the AI value of non-CMM treated control group with CMM-treated groups at temperature challenge 32°C, it was found that an AI value of 0.2 was obtained for non-CMM-treated control group whereas CMMs-treated groups showed AI values ranged from 0.33 to 0.4. The increase of AI value in those CMM-treated groups means that Drosophila adults became more susceptible to noxious heat stimulation. This indicates that those identified CMMs by the larvae model possess strong and versatile antinoceiceptive activities in Drosophila adults. / In addition, reverse transcription PCR (RT PCR) analysis was performed to study the effects of CMMs on the mRNA expression of three nociceptive-related genes painless, nompC and CG4536. These three genes all belong to the Transient Receptor Potential (TRP) families and have been shown to be involved in heat response. The results indicate that the gene expression level for nompC was significantly down-regulated with fold changes ranging from 0.2 to 0.7 upon 2 hrs treatment of three aqueous CMM extracts Citrus aurantium, Angelica dahurica and Vitex trifolia. However, there is no significant difference in gene expression level for painless and CG4536. / In this study, it has been demonstrated that Drosophila are feasible to use for screening CMMs with antinociceptive activity. While the data of the relative gene expression level for those target genes observed in this study may also serve as biomarkers for providing more evidence to investigate drugs have antinociceptive effects. In the future, such information paves the way for further development in the study of antinociceptive drugs. / Nociception is the reception of signals in the central nervous system (CNS) triggered by specialized sensory receptors which received stimuli such as electrical, thermal, mechanical, or chemical and response to escape from danger. Similar to humans, the fruitfly Drosophila display evolutionarily conserved nociceptive response that makes it suitable for in vivo nociceptive study. In this study, Drosophila larvae were used as initial screening model to investigate the antinociceptive effect that was caused by 61 randomly selected Chinese Medicinal Materials (CMMs). Upon noxious heat stimulation, 73% of larvae in the control group produced a stereotypical rolling behavior within 1 s. Among those tested CMMs, the results indicated that 4 aqueous CMMs extracts from Citrus aurantium L. (family: Rutaceae), Angelica dahurica (Fish. ex Hoffm.) Benth. et Hook (family: Umbelliferae), Vitex trifolia L. var. simplicifolia Cham. (family: Verbenaceae) and Panax notoginseng (Burk.) F. H. Chen (family: Araliaceae) were found to have strong antinociceptive effect on Drosophila larvae since less than 40% of the larvae have produced stereotypical rolling behavior within 1 s upon noxious heat stimulation. / "September 2007." / Advisers: Ming Liang Song; Ho Yin Edwin Chan. / Source: Dissertation Abstracts International, Volume: 69-08, Section: B, page: 4768. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (p. 134-139). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts in English and Chinese. / School code: 1307.
110

Mechanisms of Bak Foong Pills in the treatment of dysmenorrhoea. / CUHK electronic theses & dissertations collection

January 2005 (has links)
Dysmenorrhoea, defined as cramping pain in the lower abdomen occurring during menstruation, is known to affect up to 90% of women of childbearing age to varying degrees. The underlying causes of this condition are believed to be due to a number of factors, but are mainly attributed to increased myometrial activity, increased prostaglandin production and hormonal influences. Although there are pharmaceutical treatments available, they mainly concentrate on symptomatic relief, with the main treatment being the use of non-steroidal anti-inflammatory drugs (NSAIDs) to directly relieve the pain. Other treatments include the use of the combined oral contraceptives which are believed to influence myometrial contractility via regulation of hormonal activity. However due to the gastric and contraceptive side effects of these treatments respectively, other alternative treatments are becoming increasingly popular. One such treatment is the use of Bak foong pills (BFP), a traditional Chinese medicine used in China for the treatment of various gynaecological disorders including primary dysmenorrhoea. The aims of the current project were therefore to highlight the major beneficial effects of BFP and attempt to elucidate its major mechanisms of action in treating dysmenorrhoea. / The study demonstrated that BFP's anti-dysmenorrhoeal properties were due to a combination of hormonal, myometrial relaxant and analgesic effects. Treatment of mice with BFP caused an estrogen-like effects as demonstrated with increased cystic fibrosis transmembrane conductance regulator (CFTR) mRNA expression. Furthermore, serum estrogen and progesterone levels were also elevated in BFP treated rats. BFP was also able to significantly reduce myometrial contractions, indicating that BFP's anti-dysmenorrhoeal effect may be aided by reduced contractility of the myometrium following treatment. The uterine relaxation caused by BFP was not dependant on increases in nitric oxide or cAMP, but appeared to affect calcium mobilization. Investigation of the analgesic effect of BFP, assessed using a visceral pain model in mice showed that following sub-chronic (72 hour) treatment with BFP, there was a significant reduction in pain response, demonstrating that BFP had direct analgesic effect. (Abstract shortened by UMI.) / Rowlands Dewi Kenneth. / "July 2005." / Adviser: Hsiao Chang Chan. / Source: Dissertation Abstracts International, Volume: 67-07, Section: B, page: 3533. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (p. 148-165). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / School code: 1307.

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