Made available in DSpace on 2014-12-17T14:03:39Z (GMT). No. of bitstreams: 1 CristinaIO_DISSERT.pdf: 1652503 bytes, checksum: 5827ebf97f52c6102acf9da751e47460 (MD5) Previous issue date: 2012-12-03 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / American visceral leishmaniasis (AVL), caused by Leishmania infantum chagasi (L.i.chagasi), stands as a public health problem in Brazil, with human and canine cases related in all states..Lipid metabolism can be modified in several status of infection. For example, experimental studies show that the cholesterol is necessary to internalization and replication of L.i.chagasi in macrophages through caveolar domains. Patients with AVL present low levels of cholesterol and a visible triglycerides increase. This work aimed to evaluate the lipid metabolism in several post-infection status by L.i.chagasi, including individuals with symptomatic infection (AVL), and asymptomatic. The levels of cholesterol, triglycerides, HDL and reactive C protein, were measured. Individuals with AVL were compared with individuals with assymptomatic infection and presented low levels of total cholesterol (128 ? 6.180 mg/dL vs. 158 ?5.733 mg/dL, p=0.0001), HDL (29 ? 1.746 mg/dL vs. 37 ? 1.647 mg/dL, p=0.0001), increased levels of triglycerides (149.5 mg/dL ? 12.72 vs. 78.00 ? 10.43 mg/dL, p=0.0095) and higher levels of reactive C protein (1.750? 0.4939 mg/dL vs. 0.40 ? 0.1707 mg/dL; p=0.0001). The expression of genes related to lipid metabolism, such as LXR-a, LXR-b, PPAR-a, PPAR-d, PPAR-g and APOE was evaluated by real time PCR. A reduction in the expression of those genes was found in the group of AVL patients corroborating the serum levels of the metabolites earlier quantified. Our findings suggest a modulation of metabolism of lipids, in the chronic phase of AVL, this could facilitate the survival of leishmania, due to the known reduction on the ability of macrophages in presenting antigens efficiently to the T cells due to the reduction in the cholesterol available, it results in a subversion of the host immunity. / A leishmaniose visceral americana (LVA), causada pela Leishmania infantum chagasi (L.i.chagasi) permanece como um problema de sa?de p?blica no Brasil. A LVA cursa com altera??es bioqu?micas e hematol?gicas que podem resultar em caquexia e predisposi??o a infec??es secund?rias. O metabolismo lip?dico pode ser alterado em resposta ? infec??o, sendo que alguns microorganismos podem induzir estas altera??es.. Estudos experimentais mostram que o colesterol ? necess?rio para a internaliza??o e replica??o de L.i.chagasi em macr?fagos. Este trabalho teve como objetivo avaliar se o metabolismo lip?dico sofre altera??es em diversos status p?s-infec??o por L.i.chagasi. Os n?veis de colesterol, triglicer?deos, HDL, prote?na C reativa foram quantificados. A express?o de genes relacionados ao metabolismo lip?dico, como LXR-a, LXR-b, PPAR-a, PPAR-d, PPAR-g e APOE foi determinada por PCR em tempo real. Indiv?duos com LVA quando comparados com indiv?duos com infec??o assintom?tica apresentaram baixos n?veis de coleste rol total (128 ? 6,180 mg/dL vs. 158 ?5,733 mg/dL, p=0,0001), HDL (29 ? 1,746 mg/dL vs. 37 ? 1,647 mg/dL, p=0,001); eleva??o nos n?veis de triglicer?deos (149,5 mg/dL ? 12,72 vs. 78,00 ? 10,43 mg/dL, p=0,0095) e de prote?na C reativa (1,750? 0,4939 mg/dL vs. 0,40 ? 0,1707 mg/dL; p=0,0001). A express?o de genes envolvidos no metabolismo de lip?dos apresentou diminui??es de 3,5 vezes em pacientes com LVA, no caso de PPAR-g. Para outros genes, como PPAR-a, esta diminui??o de express?o chegou a 27,70 vezes para pacientes com LVA em rela??o ao grupo controle. Estes achados podem explicar os n?veis s?ricos alterados de lip?dios para os indiv?duos com LVA. Estes resultados sugerem que a cronicidade da infec??o por Leishmania resulta em uma modula??o do metabolismo de lip?dios, com inibi??o da s?ntese de colesterol. Isso pode facilitar a sobreviv?ncia da leishmania, por potencialmente resultar em redu??o da habilidade dos macr?fagos em apresentar ant?genos eficientemente para c?lulas T frente ? redu??o de colesterol dispon?vel, e isso resulta em uma subvers?o da imunidade do hospedeiro.

Submitted by PPG Biologia Celular e Molecular (bcm@pucrs.br) on 2018-04-17T11:40:30Z No. of bitstreams: 1 KELLY_GOULART_LIMA_TES.pdf: 13016256 bytes, checksum: b2a78492cbc6e285501d0eed5538a8ee (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-05-04T16:48:04Z (GMT) No. of bitstreams: 1 KELLY_GOULART_LIMA_TES.pdf: 13016256 bytes, checksum: b2a78492cbc6e285501d0eed5538a8ee (MD5) / Made available in DSpace on 2018-05-04T16:56:22Z (GMT). No. of bitstreams: 1 KELLY_GOULART_LIMA_TES.pdf: 13016256 bytes, checksum: b2a78492cbc6e285501d0eed5538a8ee (MD5) Previous issue date: 2018-03-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Octyl gallate (OG) is an antioxidant used in the food, cosmetic and medications industries that has shown antitumor effect on cell lineage of leukemia, melanoma and B cell lymphoma, as well as on animal model of pulmonary metastasis. Hepatocellular carcinoma (HC) is the main primary liver cancer affecting the world's population. Although surgical resection, ablation and liver transplantation are curative options, few patients are eligible for these therapies. The application of these therapeutic options is indicated only in the early stages of the disease and unfortunately most patients are diagnosed at advanced stage. In addition, there are reports in the literature of resistance to the only drug approved for systemic treatment, sorafenibe. In this study, we investigated the effect of OG on cell proliferation and lipidic metabolism in hepatocellular carcinoma HepG2 cells. Moreover, we developed a protocol for the quantitative evaluation of lipid droplets. We report, for the first time, that treatment with OG for 24 h inhibited HepG2 cell growth by decreasing mitochondrial activity and mass, which led to the reduction of ATP levels. This reduction in the energy supply triggered a decrease in Ki67 protein expression, leading to cycle arrest in S phase. In addition, the use of two treatments with OG with interval 24 hours induced loss of mitochondrial functionality and apoptosis without inducing resistance. These results showed that OG targets the mitochondria and is a candidate for new research on therapies for CH. We also report, for the first time, the effect of OG on lipidic metabolism, since our results showed that OG was able to increase the amount of lipids, triglyceride levels and the area of lipid droplets without involving the mTOR/SREBP-1c signaling pathway or modification of PPAR-? and PPAR-? gene expression. As the ability of OG to inhibit mitochondrial activity and induce apoptosis is known, it is strongly suggested that reduction of mitochondrial fatty acid b-oxidation is involved in the OG mechanism in the accumulation of lipids. We also describe, for the first time, a protocol for the quantitative evaluation of lipid droplets using confocal laser scanning microscopy that compared to conventional fluorescence microscopy, provided great gain in the quality of the images. / O galato de octila (GO) ? um antioxidante utilizado na ind?stria de alimentos, cosm?ticos e de medicamentos que tem mostrado efeito antitumoral em linhagem celular de leucemia, melanoma e linfoma de c?lulas B, assim como em modelo animal de met?stase pulmonar. O Carcinoma hepatocelular (CH) ? o principal c?ncer prim?rio hep?tico que afeta a popula??o mundial. Embora a ressec??o cir?rgica, a abla??o e o transplante hep?tico sejam considerados terapias curativas, poucos pacientes s?o eleg?veis. A aplica??o dessas op??es terap?uticas ? indicada somente para est?gios iniciais da doen?a e infelizmente a maioria dos pacientes ? diagnosticado em est?gio avan?ado. Al?m disso, existem relatos na literatura de resist?ncia ? ?nica droga aprovada para tratamento sist?mico, o sorafenibe. Nesse estudo, n?s investigamos o efeito do GO sobre a prolifera??o celular e metabolismo lip?dico nas c?lulas de carcinoma hepatocelular HepG2. Al?m disso, desenvolvemos um protocolo para avalia??o quantitativa de gotas lip?dicas. N?s reportamos, pela primeira vez, que o tratamento com GO por 24 horas inibiu a prolifera??o das c?lulas HepG2 por reduzir a atividade e massa mitocondrial, que levou ? redu??o dos n?veis de ATP. Essa redu??o no fornecimento de energia desencadeou a diminui??o na express?o da prote?na Ki67, levando ? parada do ciclo celular em fase S. Al?m disso, o uso de dois tratamentos com GO com intervalo de 24 horas induziu perda da funcionalidade mitocondrial e apoptose, sem induzir resist?ncia. Esses resultados mostraram que o GO tem como alvo a mitoc?ndria, sendo um candidato para novas pesquisas sobre terapias para o CH. Reportamos tamb?m, pela primeira vez, o efeito do GO sobre o metabolismo lip?dico, visto que nossos resultados mostraram que o composto foi capaz de aumentar a quantidade de lip?deos, os n?veis de triglicer?deos e a ?rea de gotas lip?dicas, sem envolver a via de sinaliza??o mTOR/SREBP-1c ou a modifica??o da express?o dos genes PPAR-? e PPAR-?. Uma vez que a capacidade do GO em inibir a atividade mitocondrial e induzir a apoptose ? conhecida, ? fortemente sugerido que a redu??o da ?-oxida??o mitocondrial de ?cidos graxos esteja envolvida no mecanismo do GO no ac?mulo de lip?deos. Descrevemos tamb?m, pela primeira vez, um protocolo para avalia??o quantitativa de gotas lip?dicas usando microscopia confocal de varredura a laser que comparado ? microscopia de fluoresc?ncia convencional, proporcionou grande ganho na qualidade das imagens.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-06-09T16:27:04Z No. of bitstreams: 1 ISABELE DA COSTA ANGELO.pdf: 4140152 bytes, checksum: bbd35489ea24b040be77df36cd797987 (MD5) / Made available in DSpace on 2016-06-09T16:27:32Z (GMT). No. of bitstreams: 1 ISABELE DA COSTA ANGELO.pdf: 4140152 bytes, checksum: bbd35489ea24b040be77df36cd797987 (MD5) Previous issue date: 2011-03-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The study evaluated the protein and lipid profiles of Rhipicephalus microplus engorged females after infection by Metarhizium anisopliae, Beauveria bassiana and Fusarium oxysporum. The treatments were immersion or inoculation of conidial suspension in R. microplus. The hemolymph was collected 24 and 48 hours after treatment. The cell-free hemolymph was separated of hemocytes by centrifugation and hemocytes resuspended in phosphate buffer pH 7.2. The amount of total protein was determined in both fractions of hemolymph and hemocytes were quantified. The cell-free hemolymph was filtered through a 100 kDa and 10 kDa membranes, and analyzed by electrophoresis and liquid chromatography (HPLC). The proteome of cell-free hemolymph (treatment by injection) was evaluated by 2DPAGE. Changes were observed in amount total protein and the amount of hemocytes, but no difference was observed in the electrophoretic profile (1D-PAGE) of the cell-free hemolymph. In haemocytes, the entomopathogens reduced the amount of serpins, while F. oxysporum caused increased. In 2D-PAGE variations were observed in both expression and presence/absence of protein between the groups. The cell-free hemolymph antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus and against the fungus used in the treatment of engorged ticks. The hemolymph collected 48 hours after the immersion treatment with B. bassiana apresented activity anti-B. bassiana with 48 hours of evaluation. This hemolymph was subjected to Superose column to HPLC and peak was collected and analyzed on the analytical column C18. The fractions were collected from the C18 and its apresented activity anti-B. bassiana, but showed no activity against Candida albicans. These fractions were analyzed by Maldi-Tof and most of them had in common an ion with m/z 1,119.5; however, other ions may be involved with this activity antimicrobial. The lipids present in cell-free hemolymph, in the hemocyte and fat body were extracted and analyzed by thin layer chromatography (TLC) or HPTLC for neutral lipids and phospholipids. The classes of neutral lipids in the cell-free hemolymph were cholesterol ester, cholesterol (CHO) and fatty acids (FA), which have varied depending on the fungus used, type of treatment and observation time. Phospholipids found were phosphatidylcholine and phosphatidylethanolamine, its were not significantly altered after the fungal infection. In hemocytes, the same classes of lipids were found and B. bassiana modified phospholipids, while M. anisopliae s.l. altered FA and CHO. The fat body showed, in addition to these classes of neutral lipids, the triglycerides, which increased significantly 48 hours after inoculation with M. anisopliae s.l. The lipase activity in fat body was measured and it was demonstrated that increased activity 48 hours after inoculation, mainly in the group inoculated with Metarhizium. Therefore, the results showed alterations related to the proteins expression in the hemocytes and the cell-free hemolymph after inoculation with fungi, immunosuppression of hemocytes and antimicrobial peptides induction after infection with B. bassiana, besides changes in the lipid profile of R. microplus after infection. However, further studies are necessary to understand these changes. / O trabalho avaliou os perfis prot?ico e lip?dico de f?meas ingurgitadas de Rhipicephalus microplus ap?s infec??o com Metarhizium anisopliae s.l., Beauveria bassiana ou Fusarium oxysporum. Os tratamentos foram imers?o ou inocula??o da suspens?o conidial em R. microplus. A hemolinfa foi coletada 24 e 48 horas ap?s os tratamentos. O plasma foi separado dos hem?citos por centrifuga??o e os hem?citos resuspensos em tamp?o fosfato pH 7,2. A concentra??o de prote?na total foi determinada em ambas as fra??es da hemolinfa e os hem?citos quantificados. O plasma da hemolinfa foi filtrado em membrana de 100 kDa e 10 kDa, sendo analisados por eletroforese e cromatografia l?quida de alta efici?ncia (CLAE). O proteoma do plasma da hemolinfa (tratamento por inocula??o) foi avaliado por gel 2D. Foram observadas varia??es na quantidade de prote?na total de ambas as fra??es da hemolinfa, na quantidade de hem?citos bem como na intensidade de prote?nas/pept?deos expressos no plasma da hemolinfa. Nos hem?citos, os entomopat?genos reduziram a quantidade de serpinas, enquanto F. oxysporum causou aumento. No gel 2D foram observadas varia??es na express?o bem como na aus?ncia/presen?a de prote?nas entre os grupos. O plasma da hemolinfa teve sua atividade antimicrobiana testada contra Escherichia coli e Staphylococcus aureus e contra o fungo utilizado no tratamento das f?meas ingurgitadas. A hemolinfa coletada 48 horas ap?s o tratamento por imers?o com B. bassiana apresentou atividade anti-B. bassiana com 48 horas de avalia??o. Esta hemolinfa foi submetida ? coluna Superose de CLAE e o pico coletado analisado na coluna anal?tica C18. As fra??es coletadas da C18 apresentaram atividade anti-B. bassiana, por?m n?o apresentaram atividade contra Candida albicans. Essas fra??es foram analisadas por Maldi-Tof e a maioria delas apresentou um ?on com raz?o m/z 1.119,5; no entanto, outros ?ons podem estar envolvidos com essa atividade antimicrobiana. Os lip?deos presentes no plasma da hemolinfa, nos hem?citos e no corpo gorduroso foram extra?dos e analisados por cromatografia em camada delgada (CCD) ou CCD de alta performance para lip?deos neutros e fosfolip?deos. As classes de lip?deos neutros encontradas no plasma da hemolinfa foram colesterol-?ster, colesterol (CHO) e ?cidos graxos (AG), que sofreram altera??es em fun??o do fungo utilizado, tipo de tratamento e tempo de observa??o. Os fosfolip?deos encontrados foram fosfatidilcolina e fosfatidiletanolamina, que n?o foram significativamente alterados ap?s a infec??o f?ngica. Nos hem?citos, as mesmas classes de lip?deos foram encontradas e B. bassiana alterou os fosfolip?deos, enquanto M. anisopliae s.l. alterou os AG e CHO. O corpo gorduroso apresentou, al?m destas classes de lip?deos neutros, o triacilglicerol, que aumentou significativamente 48 horas ap?s a inocula??o com M. anisopliae s.l. A atividade lipase no corpo gorduroso foi mensurada, sendo evidenciado um aumento 48 horas ap?s a inocula??o, principalmente no grupo inoculado com Metarhizium. Portanto, os resultados demonstraram altera??es na express?o de prote?nas no plasma da hemolinfa e nos hem?citos ap?s inocula??o com os fungos, imunossupress?o dos hem?citos, indu??o de pept?deos com atividade antimicrobiana ap?s infec??o com B. bassiana, al?m de altera??es no perfil lip?dico de R. microplus ap?s infec??o. No entanto, maiores estudos s?o necess?rios para o entendimento dessas altera??es.

Made available in DSpace on 2014-12-17T14:13:46Z (GMT). No. of bitstreams: 1 MariaFPB_TESE.pdf: 831507 bytes, checksum: 16c85e1c11c77cb917991582252e33d1 (MD5) Previous issue date: 2013-03-20 / A S?ndrome de Berardinelli-Seip (SBS) ? um dist?rbio raro do metabolismo dos lip?dios, caracterizada pela aus?ncia quase total de tecido adiposo subcut?neo, hipertrigliceridemia, hipoleptinemia e diabetes insulino resistente ou lipoatr?fico. Sua etiologia envolve implica??es hipotal?micas, altera??es nos receptores de insulina e muta??es nos genes AGPAT2, Gng3lg, CAV1 e PTRF. O tecido adiposo secreta diversas subst?ncias, tais como: leptina, resistina, adiponectina, ester?ides, TNF , IL-6, PAI-1, angiotensinog?nio, IGF-1. Muitas delas est?o associadas ao diabetes mellitus tipo 2, obesidade e hipertens?o. Os PPARs s?o fatores transcricionais pertencentes ? superfam?lia de receptores nucleares ligantes ativados. Sabe-se que o PPAR , ? importante para o metabolismo lip?dico e glic?dico e que o ligante natural do PPAR ? derivado do ?cido graxo. Nesse sentido, foram avaliados 24 pacientes portadores da SBS, provenientes do Estado do Rio Grande do Norte, com a mediana das idades de 18,5 anos (0,55 a 47 a), sendo 9 (37,5 %) do g?nero masculino e 15 (62,5 %) do g?nero feminino. Quanto ao grupo ?tnico, foram classificados em caucas?ides (brancos) 21 (87,5 %) e negr?ides 3 (12,5 %) pacientes. Foram feitas avalia??es cl?nico-endocrinol?gica, bioqu?mica, hormonal, molecular e o estudo dos polimorfismos Adiponectina ADIPOQ, PPARγ2 Pro12Ala, LPL-PvuII, APOC3-SstI e LDLR-AvaII em portadores da SBS. Nesta popula??o n?s n?o encontramos nenhuma associa??o de par?metros lip?dicos e glic?dicos com os polimorfismos LPL-PvuII, APOC3-SstI e LDLR-AvaII. Por?m, observamos associa??o entre Adiponectina ADIPOQ e PPARγ2 Pro12Ala e n?veis lip?dicos mais elevados, sugerindo um papel biol?gico para estes fatores, indicando estudos mais aprofundados

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:34:43Z No. of bitstreams: 5 cli.pdf: 2071528 bytes, checksum: dac792d5c097644e675cbf4a0a1ef994 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T10:21:37Z (GMT) No. of bitstreams: 5 cli.pdf: 2071528 bytes, checksum: dac792d5c097644e675cbf4a0a1ef994 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Made available in DSpace on 2015-02-20T10:21:37Z (GMT). No. of bitstreams: 5 cli.pdf: 2071528 bytes, checksum: dac792d5c097644e675cbf4a0a1ef994 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) Previous issue date: 2013-02-19 / Myrciaria cauliflora (jabuticabeira) ? uma esp?cie frut?fera, nativa do Brasil e bastante cultivada em pomares dom?sticos de Minas Gerais. Por?m, pouco se conhece sobre a composi??o qu?mica e efeitos biol?gicos dos res?duos de seus frutos. Diante disso, o presente trabalho teve como objetivo avaliar a composi??o qu?mica, o potencial antioxidante (in vitro) e hipolipid?mico (in vivo) da farinha das cascas do fruto de Myrciaria cauliflora. Para tanto, frutos (jabuticabas) maduros foram despolpados manualmente e suas cascas secas e trituradas at? a obten??o de uma farinha homog?nea (FCJ). Avaliou-se sua composi??o centesimal, conte?do mineral, fen?licos totais, flavon?ides e antocianinas, assim como sua atividade antioxidante in vitro atrav?s de diferentes m?todos (DPPH, ABTS?+, FRAP e ?-caroteno/?cido linol?ico). A FCJ foi submetida ? extra??o hidroalco?lica e os extratos fracionados em Cromatografia em Coluna (CC). Os fitoconstituintes isolados foram caracterizados por IV e RMN. O potencial hipolipid?mico foi avaliado em grupos de ratos machos alimentados com dietas semipurificadas, sendo a dieta Padr?o (PDR) baseada na AIN93M, a dieta Controle (CTRL) semelhante ? PDR e suplementada com 7% de banha de porco, e tr?s dietas experimentais semelhantes ? CTRL, por?m suplementadas com 7, 10 e 15% de FCJ (JAB1, JAB2 e JAB3, respectivamente). N?veis s?ricos e hep?ticos de colesterol, bem como n?veis s?ricos de HDL, triglicer?deos e glicose foram avaliados. A excre??o fecal de lip?deos foi avaliada tamb?m. A FCJ foi classificada como fornecedora de alto teor de fibras (15,26%). Os teores de fen?licos totais (1895 mg ?cido g?lico/100 g), flavon?ides (8,960 g de catequina /100g) e antocianinas (0,6823 g de cianidina-3-glicos?deo/100g) foram altos assim como a atividade antioxidante verificada pela captura de radicais-livres DPPH (3,184 g de FCJ/g DPPH) e ABTS?+ (1017 ?mol Trolox/g de FCJ), redu??o dos ?ons Fe+3 (167,7 mM de Fe2SO4/100g) e capacidade de inibi??o do branqueamento do ?-caroteno (75,96% de inibi??o para a concentra??o de 1000 mg/L). O ?cido c?trico, o sitosterol e o sitosterol-D-glicopiranos?deo foram isolados, sendo, os dois ?ltimos, citados pela primeira vez nesta esp?cie. A inclus?o da FCJ, nas tr?s propor??es, reduziu o colesterol total (CT) e os triglicer?deos (TG) s?ricos em rela??o ? dieta CTRL. A dieta JAB3 elevou os n?veis de HDL s?ricos e reduziu os de colesterol hep?tico em rela??o ? CTRL. A dieta JAB3 reduziu a glicose s?rica em compara??o ? JAB2, mas essa diferen?a n?o foi significativa em rela??o ? PDR, ? CTRL ou ? JAB1. N?o houve diferen?a na excre??o fecal de lip?deos entre os grupos experimentais. Os resultados obtidos poder?o servir de aux?lio para o melhor aproveitamento deste res?duo e compreens?o de seus efeitos metab?licos al?m de contribuir para garantir a seguran?a da sua ingest?o como alimento. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT Myrciaria cauliflora (jabuticabeira) is a fruit specie, native in Brazil and created mainly in domestic gardens in Minas Gerais. However, little is known about its chemical composition and biological effects in the fruit residue. In front of this, the present work had as a goal to evaluate the chemical composition, antioxidant potential (in vitro) and hypolipidemic (in vivo) of Myrciaria cauliflora fruit peel flour (FCJ). For that, mature fruits (jabuticaba) were pulped manually and its peel was dried and crushed to obtain a homogeneous flour (FCJ). There had been evaluated the proximate composition, mineral content as well as total phenolic, flavonoids and anthocyanins as their antioxidant activity in vitro through different methods (DPPH, ABTS?+, FRAP and ?-caroten/acid linoleic). The FCJ was also subjected to extraction with water-alcohol extracts fractionated in column chromatography (CC). The phytochemicals isolated were characterized IR and NMR. The lipid-lowering activity was evaluated in groups of male rats, fed with semipurified diets, being a PDR diet based on AIN93M; a CTRL diet based on PDR, but added 7% of pork lard; and three experimental diets similar to CTRL, but added MPF at 7, 10 and 15% (JAB1, JAB2 and JAB3, respectively). Serum and liver cholesterol as well as serum levels of HDL, triglycerides and glucose were evaluated. Fecal output of lipids was also evaluated. The FCJ was classified as a supplier of high-fiber (15,26%). The levels of total phenolic (1895 mg gallic acid/100 g), flavonoids (8,960 g de catechin /100g) and anthocyanins (0,6823 g de cyanidin-3-glucoside/100g) were high as the antioxidant activity verified to capture of free radicals DPPH (3,184 g de FCJ/g DPPH) and ABTS?+ (1017 ?mol Trolox/g de FCJ), to reduction of ions Fe+3 (167,7 mM de Fe2SO4/100g) and capacity in inhibition of whitening of ?-caroten (75,96% of inhibition of concentration of 1000 mg/L). The citric acid, sitosterol and sitosterol-?D-glucopyranoside were isolated, being the last two, mentioned in this specie for the first time. The inclusion of the MPF, at the three ratios reduced serum cholesterol and triglycerides compared to CTRL. JAB3 diet raised serum HDL cholesterol and reduced liver cholesterol compared to CTRL. JAB3 diet reduced serum glucose compared to JAB2, but this difference was not significant compared to PDR, CTRL or JAB1. There was no difference in fecal output of lipids between the groups. The results obtained may serve as an aid to better utilization of residue and to understand the MFP metabolic effects in addition to ensure the safety of its intake as a food.