Indiana University-Purdue University Indianapolis (IUPUI) / Cancer is still among those diseases that prominently contribute to the numerous deaths that are caused each year. But as technology and research is reaching new zeniths in the present times, cure or early detection of cancer is possible. The detection of rare cells can help understand the origin of many diseases. The current study deals with one such technology that is used for the capture or effective separation of these rare cells called Lab-on-a-chip microchip technology. The isolation and capture of rare cells is a problem uniquely suited to microfluidic devices, in which geometries on the cellular length scale can be engineered and a wide range of chemical functionalizations can be implemented. The performance of such devices is primarily affected by the chemical interaction between the cell and the capture surface and the mechanics of cell-surface collision and adhesion. This study focuses on the fundamental adhesion and transport mechanisms in rare cell-capture microdevices, and explores modern device design strategies in a transport context. The biorheology and engineering parameters of cell adhesion are defined; chip geometries are reviewed. Transport at the microscale, cell-wall interactions that result in cell motion across streamlines, is discussed. We have concentrated majorly on the fluid dynamics design of the chip. A simplified description of the device would be to say that the chip is at micro scale. There are posts arranged on the chip such that the arrangement will lead to a higher capture of rare cells. Blood consisting of rare cells will be passed through the chip and the posts will pose as an obstruction so that the interception and capture efficiency of the rare cells increases. The captured cells can be observed by fluorescence microscopy. As compared to previous studies of using solid microposts, we will be incorporating a new concept of cylindrical shell micropost. This type of micropost consists of a solid inner core and the annulus area is covered with a forest of silicon nanopillars. Utilization of such a design helps in increasing the interception and capture efficiency and reducing the hydrodynamic resistance between the cells and the posts. Computational analysis is done for different designs of the posts. Drag on the microposts due to fluid flow has a great significance on the capture efficiency of the chip. Also, the arrangement of the posts is important to contributing to the increase in the interception efficiency. The effects of these parameters on the efficiency in junction with other factors have been studied and quantified. The study is concluded by discussing design strategies with a focus on leveraging the underlying transport phenomena to maximize device performance.

Indiana University-Purdue University Indianapolis (IUPUI) / The purpose of this thesis is to use experimental methods to seek deeper understanding and better performance in the self-circulating self-regulating microfluidic gas generator initially developed in Dr. Zhu’s group, by changing the major features and dimensions in the reaction channel of the device. In order to effectively conduct experiments described above, a microfabrication method that is capable of making new microfluidic devices with low cost, short time period, as well as relatively high accuracy was needed first. Developing such a fabrication method is the major part of this thesis. We initially used patterned polymer films and glass slide, and bonded them together by sequentially aligning and stacking them into a microfluidic device with patterned double-sided tapes. Later we developed a more advanced microfabrication method that used only patterned polystyrene (PS) films. The patterned PS films were obtained from a digital cutter and they were bonded into a microfluidic device by thermopress bonding method that required no heterogeneous bonding agents. This new method did not need manual assembly which greatly improved its precision (~ 100 µm), and it used only PS as device material that has favorable surface wetting property for microfluidics applications. In order to find the optimized microfluidic channel design to improve gas generating performance, we've designed and fabricated microfluidic devices with different channel dimensions using the PS fabrication method. Based on the gas generation testing results of those devices, we were able to come up with the optimal dimensions for the reaction channel that had the best gas generation performance. To obtain a more fundamental understanding about the working mechanism of our device and its bubble dynamics, we have conducted ultrafast X-ray imaging test at Advanced Photon Source (APS), Argonne National Laboratory. High speed (100 KHz) phase contrast images were captured that allowed us to observe directly inside the reaction channel on the cross section view during the self-circulating catalytic reaction. The images provided us with lots of insightful information that in turn helped the dimensional improvement for the microchannel design. The 100 KHz high speed images also gave us useful information about the dynamics of bubble development on a catalyst bed, such as growth and merging of the bubbles.

Indiana University-Purdue University Indianapolis (IUPUI) / In this study, a novel model for manipulating cancer blood cells based on multi-stage micro channels under varied low field concepts is proposed. Steering Device approach was followed to manipulate the cancer cells based on their various differential potentials across their membranes. The proposed approach considers the size and the surface potential as well as the iso electronic structure of the cells. These research objectives emphasize the separation of the cells in the blood stream, and differentiates various blood cells and tumors for further analysis within the microfluidic channels. The dimensions of the channel sets the required electric field for manipulating the cancer cells within the channels using low electrode voltage function. The outcomes of this research may introduce a new diagnostic approach of finding the minimum residual disease (MRD) scans, early detection and analysis scans. This thesis provides a mathematical model, detailing the theory of the cell sorting device, manipulating the blood cancer cells and design of the device structure are also detailed, leading to the optimum research parameters and process. A Computer Aided Design (CAD) was used to model the multi-cell sorting lab-on-chip device, details of hardware and software were used in the simulation of the device various stages. Reverse engineering to configure the potentials for sorting mechanism needs is discussed. The thesis work also presents a comparative study of this sorting mechanism and the other commercially available devices. The practical model of the proposed research is laid out for future consideration.