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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Estudo alelopático, fitoquímico e genotóxico de extratos aquosos de Aspidosperma pyrifolium Mart. e Combretum leprosum Mart. em Allium cepa / Allelopathic study, phytochemical and genotoxic of aqueous extracts of Aspidosperma pyrifolium Mart. And Combretum leprosum Mart. in Allium cepa

Pereira, José Carlos da Silveira 27 August 2015 (has links)
Submitted by Lara Oliveira (lara@ufersa.edu.br) on 2017-08-07T21:51:39Z No. of bitstreams: 1 JoséCSP_DISSERT.pdf: 861903 bytes, checksum: 5f78fa00a8f44435ec8fd2bebd231afc (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-08-17T22:38:54Z (GMT) No. of bitstreams: 1 JoséCSP_DISSERT.pdf: 861903 bytes, checksum: 5f78fa00a8f44435ec8fd2bebd231afc (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-08-17T22:40:52Z (GMT) No. of bitstreams: 1 JoséCSP_DISSERT.pdf: 861903 bytes, checksum: 5f78fa00a8f44435ec8fd2bebd231afc (MD5) / Made available in DSpace on 2017-08-17T22:40:59Z (GMT). No. of bitstreams: 1 JoséCSP_DISSERT.pdf: 861903 bytes, checksum: 5f78fa00a8f44435ec8fd2bebd231afc (MD5) Previous issue date: 2015-08-27 / The Caatinga has great plant diversity, but few native species have phytochemical, allelopathic and genotoxic studies. Aspidosperma pyrifolium and Combretum leprosum are examples of plants of great importance ethnopharmacological that lacking of toxicological studies. The objective was to evaluate the allelopathic influence, cytotoxicity and genotoxicity of aqueous extracts of A. pyrifolium and C. leprosum on germination and early development of commercial seed of Allium cepa (indicator organism) and describing their biochemical profiles. 50 seeds of A. cepa were placed in the presence of aqueous extracts of these plants in concentrations of 200, 400 and 800 mg/L, with four replicates each, the following variables were evaluated: germination, germination speed, length and dry matter of roots, hypocotyl and seedling, seedling biomass density and mitotic index. Qualitative and quantitative tests were performed on various classes of metabolites to describe the biochemical profile. The results of the extracts groups did not differ from negative control group, for the germination characteristics, indicating that the extracts do not have allelopathic effect. However, the mitotic index was identified sublethal cytotoxicity of aqueous extract of C. leprosum, with 200 mg/L. Biochemical profiles identified novel substances for both species. However, due to possible synergistic effects of the extracts more studies are needed to better phytochemical characterization of both species / A Caatinga apresenta grande diversidade vegetal, mas poucas espécies nativas possuem estudos fitoquímicos, alelopáticos e genotóxico. Aspidosperma pyrifolium e Combretum leprosum são exemplos de plantas de grande importância etnofarmacológica que carecem de estudos toxicológicos. O objetivo foi avaliar a influência alelopática, citotoxidade e genotoxidade de extratos aquosos de A. pyrifolium e C. leprosum sobre a germinação e desenvolvimento inicial de sementes comerciais de Allium cepa L. (organismo-teste) e descrever seus perfis fitoquímicos. 50 sementes de A. cepa foram colocadas na presença dos extratos aquosos dessas plantas nas concentrações de 200, 400 e 800 mg/L, com quatro réplicas cada, sendo avaliadas as seguites variáveis: germinação, velocidade de germinação, comprimento e matéria seca de raízes, hipocótilo e plântula, densidade de biomassa de plântula e índice mitótico. Foram realizados testes qualitativos e quantitativos em diversas classes de metabólitos para a descrição do perfil fitoquímico. Os resultados dos grupos dos extratos não diferiram estatisticamente do grupo controle negativo, quanto às características germinativas, indicando que os extratos não possuem efeito alelopático. No entanto, pelo índice mitótico foi identificado citotoxidade subletal do extrato aquoso de C. leprosum, com 200 mg/L. Os perfis fitoquímicos identificaram substâncias inéditas para ambas as espécies. Entretanto, devido a possíveis efeitos sinergéticos dos extratos são necessários mais estudos para melhor caracterização fitoquímica de ambas as espécies / 2017-08-07

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