ABSTRACT The City of Morro Bay, California – Sign Ordinance Update and Embarcadero District Tourism-Oriented Directional Sign Plan Erik Berg-Johansen This report includes a Draft Sign Ordinance, a Tourism-Oriented Directional Sign Plan, and a background report for each product. The final products were created thought a process involving extensive research, community outreach, and detailed discussions among City of Morro Bay staff. The Sign Ordinance It was discovered that signs are important to business owners and residents due to their effect on both economic and aesthetic issues in communities. This report documents research of scholarly articles, case studies, and community outreach efforts. This report includes survey results and analysis that reveal the opinions of Morro Bay business owners, and also their ideas in regards to the sign ordinance update. According to many business owners, the current sign ordinance is convoluted, virtually unenforced, and unfairly applied. It was the goal of this project to hear what the community desires, and then apply this knowledge to a proposal that residents and business owners in Morro Bay approve of. The proposed sign ordinance aims to be fair and user-friendly, while ultimately enhancing community character and aesthetic quality in the future. Tourism-Oriented Directional Sign Plan The Tourism-Oriented Directional Sign Plan was created to promote tourism in the City of Morro Bay and negate the need for A-frame sign use in the Embarcadero District. The plan includes three alternatives that are intended to spur discussion among the Planning Commission when the proposal is presented. The background report associated with this plan is intended to provide the reasoning behind the proposals, and give readers of the plan background knowledge on directional signs in general. Similar to the sign ordinance background report, this report documents research of scholarly articles, case studies, and community outreach efforts.

Seagrasses are well known for the important ecological roles they play in coastal marine waters worldwide. However, the severe rate of decline observed in seagrasses this century is expected to accelerate with climate change. Conservation efforts can be improved by quantifying physiological thresholds of seagrasses and using these estimates in modeling to forecast changes in distribution. This study examines the response of eelgrass (Zostera marina L.) across current temperatures to look for early warning signs of vulnerability and to evaluate the ways we determine critical thresholds for survival. Whole eelgrass ramets, collected from three beds in Morro Bay, California, were used to develop photosynthesis-irradiance (P-I) curves from 10-20°C. Productivity was not affected by changes in temperature when traditionally measured as the light-saturated photosynthetic rate to dark respiration rate (P:R) ratio. However, photosynthesis in light-limited conditions declined at higher temperatures, suggesting a decrease in productivity when coupled with the increased respiration rates observed at higher temperatures. Irradiance thresholds increased with temperature; critical irradiance was the most sensitive to increases in temperature due to the inclusion of overnight energy use, which also increases with temperature. Measurements of root and rhizome respiration, overnight respiration, and variation across eelgrass beds reveal that these are important components to consider when calculating survival thresholds to use in modeling. Differences in physiological responses across beds suggest that some eelgrass beds operate more efficiently than others in current conditions and are likely to be more resilient to the progressing stressors of climate change. Management of eelgrass in the face of climate change will require reliable distribution forecasts, and therefore accurate estimates of physiological thresholds, to guide mitigation and restoration efforts.

The Morro Bay Watershed, which is located inSan Luis Obispo County,California, covers more than 48,000 acres of land and discharges intoMorroBaythrough the Morro Bay National Estuary (MBNE). The Chorro Creek Subwatershed consists of approximately 30,000 acres of the overall watershed. The MBNE provides an ecosystem that supports a variety of wildlife from the common sea gull to the endangered sea otter. The estuary is also home to over 200 species of birds. The operational waterfront of theMorroBayHarborwas and continues to be a strong supporter to the local economy of the City of Morro Bay. Numerous studies were conducted since the 1990s throughout the watershed to study the sedimentation of the estuary and bay and identified accelerated erosion and subsequent sedimentation as a major threat to sustainability of the bay. As a result, various Best Management Practices (BMPs) were implemented in the watershed to reduce sediment loading and transport to the bay. Localized evaluations of various BMPs have been performed to investigate effectiveness of individual BMPs. This paper consolidates this information and develops a comprehensive spatially distributed watershed simulation model (1) for detailed understanding of the erosion and sedimentation processes in the watershed; (2) to evaluate a watershed scale effectiveness of the conservation practices that were installed in the watershed; (3) to identify optimal BMP types and sites that may be used in the future to further reduce sedimentation of the bay at minimal cost; (4) to organize and document the various sources of data and studies that have been performed to date in the Chorro Creek subwatershed. Soil and Water Assessment Tool (SWAT) was used to develop the model and to evaluate the pre- and post-BMP implementation characteristics in the subwatershed. Combining the data and efforts of past BMP evaluations, land use, soil type, climate data, and streamflow data, statistical evaluations, and model sensitivity analysis will help build and calibrate a robust SWAT model that can be used to track BMP evaluation efforts, as well as other watershed management tasks. Through the evaluation of BMPs in the watershed, efforts can be made to implement the more successful BMPs in the watershed or in other similar watersheds. Sensitivity analysis was performed using a global sensitivity analysis method and streamflow and sediment yield was calibrated using the Shuffled Complex Evolution-University ofArizona.

Estuaries are dynamic environments that are strongly affected by natural variability, as well as direct and indirect anthropogenic impacts. A better understanding of the drivers of carbon fluxes and biogeochemical variability in estuarine systems is needed, particularly with the increasing threat of ocean acidification. Morro Bay in Central California is a small nationally protected estuary, with seasonally low freshwater inputs. Since 2007, the bay has experienced a significant loss of native seagrass, Zostera marina, which is an important component of the marine ecosystem. Because seagrass photosynthesis decreases carbon dioxide and increases oxygen in the water column, the loss of seagrass has the potential to substantially change short-term carbonate chemistry and long-term carbon fluxes of an estuary. The spatial variability of carbonate chemistry was measured in Morro Bay using ship-board surveys during the low-inflow summer season and measured the temporal variability by collecting samples close to the shore from July to November. Discrete samples show an increase in total alkalinity and dissolved inorganic carbon in the mid and back bay regions, historically dominated by seagrass. Slightly lower total alkalinity and dissolved inorganic carbon were observed in the Fall season compared to the low-inflow Summer season. Analysis of the relative modification of alkalinity and dissolved inorganic carbon, paired with salinity and temperature data, contributes to an understanding of the drivers of the observed carbonate variability. This understanding may provide clues to the causes and effects of observed changes to the bay with seagrass loss. More broadly, it will inform the vulnerability of other low-inflow estuaries to future acidification and highlight the role seagrasses play in mitigating local acidification.

There is a growing need to better understand the dynamics of small and medium Mediterranean low-inflow estuaries (LIEs), which is addressed here by characterizing a heat budget and associated heat transfer processes. A one-dimensional deterministic model was developed from the advection-diffusion equation and applied to Morro Bay, CA using 15-minute water property (temperature, salinity, pressure) and meteorological (wind speed and direction, air temperature, relative humidity, air pressure, irradiance) data collected over a two-year period (2020 – 2021). Seasonal variability is observed in meteorological components, water temperature, and salinity. There is strong seasonal variability in head-mouth temperature and salinity differences. Temperature differences peak in summer (daily mean 2.52 ºC, June – Sept.). Daily average salinity difference is 0.33 (hyposaline, Sept. – Apr.) with strongest gradients observed during the winter storm season following enhanced freshwater discharge. Inverse salinity develops intermittently May – Aug. Subtidal heat flux is dominated by surface heating, whose daily average is always positive (heat input). The developed model does not quantify adequate heat export from the estuary, however, a sensitivity analysis indicates that diffusive flux may be a significant heat export component. Excess heat appears to be exported to the ocean, allowing ocean-estuary temperatures to remain similar. Characterizing estuarine dynamics like these enables us to predict how Morro Bay, and other similar estuarine systems, may respond to long and short-term environmental changes, and how these responses influence estuarine circulation and environmental health.

Using an instrumented water quality network in Morro Bay Estuary, California from 2007 to 2010 (15 min sampling frequency), this study addressed the two objectives of constructing a nitrate budget and assessing the influence of sampling frequency on water quality parameters. These two objectives led to the submission of an original report of research (Appendix A) and a note (Appendix B) to peer-reviewed journals. The first objective was to characterize the high spatial and temporal variation in physical parameters and nitrate concentrations and to construct a nitrate budget quantifying sources and sinks of nitrate from the ocean, streams, and groundwater, as well as biological processes in the Estuary. Morro Bay Estuary was found to be a non-eutrophic system and a mean net exporter of nitrate, 327.15 t yr-1. Fifty-four percent of the nitrate export was attributed to nitrate sources and internal biological processing. Nitrate loading from streams contributed 37 % to the export of nitrate (124.01 t yr-1), while groundwater nitrate loading supplied a conservative estimate of 46 % of the exported nitrate (153.92 t yr-1), with a neap tide enhancement of the discharge. Denitrification, Zostera marina, and benthic macroalgae assimilation of nitrate were the dominant internal biological processes for removal and retention, but were only 35% of the total nitrate budget. The second objective was to investigate the impact of sampling frequency and sampling location on understanding dynamics in water quality by degrading a year time series of seven parameters from three water quality monitoring stations to sampling frequencies ranging from 15 minutes to 28 days. In Morro Bay Estuary, the semi-diurnal tidal cycle was the maximum component frequency driving the variability of temperature, turbidity, and dissolved oxygen concentrations. For these parameters, asymptotes were reached and sampling frequencies greater than six hours did not explain the additional variation in the parameters sampled. Whereas, salinity, turbidity, and nitrate concentrations lacked an asymptote, and decreased sampling frequencies led to increased estimated error. Sampling water quality parameters every 28 days can lead to mean annual difference of 30 – 140 % from 15 minute sample annual mean. We recommend sampling frequencies should be selected to oversample the tidal signal to at least hourly frequencies to capture diel cycles and episodic events that contribute significantly to understanding the variability in the estuarine physical and biological dynamics.

The endemic eelgrass (Zostera marina) community of Morro Bay Estuary, located on the central coast of California, has experienced an estimated decline of 95% in occupied area (reduction of 344 acres to 20 acres) from 2008 to 2017 for reasons that are not yet definitively clear. One possible driver of degradation that has yet to be investigated is the role of herbicides from agricultural fields in the watershed that feeds into the estuary. Thus, the primary research goal of this project was to better understand temporal and spatial trends of herbicide use within the context of San Luis Obispo (SLO) County and Morro Bay Watershed by analyzing data of application by mass, area, and intensity to identify herbicides with the highest potential for local environmental pollution. California Pesticide Use Annual Summary Reports (PUASR) from the years 2000 to 2017 were used to obtain data for conducting a meta-analysis to estimate total herbicide application by weight within every township, range, and section for each of the eight selected herbicides: oxyfluorfen, glyphosate, diuron, chlorthal-dimethyl, simazine, napropamide, trifluralin, and oryzalin. A second goal was to select an analytical laboratory that would be best suited for herbicide analysis of estuary sediments to determine the presence, or lack thereof, of the eight selected herbicides. Criteria of consideration in laboratory selection included herbicides detection capabilities, detection/reporting limits, testing prices, chain of custody protocols, turnaround times, and laboratory site locations. The meta-analysis yielded results showing high herbicide application rates in SLO County with glyphosate, oxyfluorfen, and chlorthal-dimethyl being identified as three herbicides of elevated risk for local environmental contamination due high rates of use by mass, by area, and/or intensity during the study timeframe. Additionally, Morro Bay Watershed exhibited moderate rates of herbicide application with chlorthal-dimethyl and glyphosate being of highest risk for contamination and accumulation within the estuary because of high application rates by mass, by area, and/or intensity. Finally, Environmental Micro Analysis (EMA) and Primus Group, Inc. (PrimusLabs) were identified as the top candidates for analytical laboratory testing of Morro Bay Estuary sediment samples to be obtained and tested for the selected herbicides. These laboratories provide superior analytical capabilities of the eight herbicides, impressive reporting limits or lower detection limits, competitive testing prices for detecting multiple constituents in multiple samples, robust chain of custody protocols, options for quick turnaround times, and laboratory site locations within California.