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published_or_final_version / Biological Sciences / Master / Master of Philosophy

The major objective of this study was to determine whether or not food production per unit space can be increased by intercropping summer squash (Cucurbita pepo L.) with mung bean (Phaseolus aureus Roxb.) or pinto bean (Phaseolus vulgaris L.), and to identify the factors associated with growth and yield of summer squash, mung bean and pinto bean under intercropping regimes. A series of experiments were conducted during the period: Summer, 1977 to February, 1980, at both the University of Arizona, Experiment Station, Marana, and in a greenhouse at the University of Arizona, Campbell Avenue Farm. Intercropping mung beans or pinto beans with summer squash in either adjacent rows or within the row did not significantly influence the bean seed yield, although adjacent row intercropping tended to outyield the within row intercropping. Summer squash yield was more significantly reduced by within row intercropping than adjacent row intercropping. Root and shoot dry weights of container grown mung beans or pinto beans were significantly reduced by intercropping with summer squash, but summer squash root and shoot dry weights were not significantly affected. Intercropping summer squash with either mung beans or pinto beans was more beneficial at low nitrogen and phosphorus fertility levels than at higher levels. Summer squash fruit and shoot dry weights per unit space increased with increase in plant population, but they were not significantly influenced by intercropping with either mung beans or pinto beans. Intercropping high population summer squash with low population mung beans or pinto beans reduced both seed and biomass yields of the beans. However, increasing the bean plant populations had no influence on their seed and biomass yields. Harvest index of mung beans or pinto beans was neither influenced by intercropping with summer squash nor by increasing the bean plant population. Leaf area per unit space increased with increase in plant population, but intercropping had no significant influence in all cases. Specific leaf weight, leaf area-to-leaf weight ratio, and leaf weight ratio were neither influenced by intercropping nor by varying the plant populations. Mung bean seed yield was significantly to highly significantly correlated with harvest index and biomass, but highly negatively correlated with leaf area index, while pinto bean seed yield was very highly correlated with biomass and harvest index. Summer squash fruit yield was significantly to highly significantly correlated with shoot dry weight, leaf area, leaf area index and specific leaf weight. Accumulations of nitrate nitrogen and/or phosphorus in the leaf petioles of mung beans, pinto beans or summer squash were neither influenced by intercropping nor by increasing the nitrogen or phosphorus fertility levels. The economic yields of field grown mung beans, pinto beans or summer squash were not significantly correlated with petiole accumulations of nitrate nitrogen and phosphorus. While summer squash exhibited autotoxicity, mung bean root leachates tended to promote growth of pinto beans and summer squash. Food production per unit space was increased by as much as 76% by intercropping summer squash with pinto beans, while intercropping summer squash with mung beans increased food production by 63%. Under certain plant combinations, dry matter yield per unit space was increased by as much as 185% by intercropping summer squash with mung beans, while intercropping summer squash with pinto beans increased the dry weight yield by as much as 81%.

Thesis (Ph.D.) - University of Queensland, 2004. / Includes bibliography.

Yeast breads were prepared with three levels of mashed sweet potato pulp and of mung bean flour. Nine combinations of mashed sweet potato pulp (10, 20, 30 percent), and of mung bean flour (4, 8, 16 percent) substitutions for wheat flour were used to determine the acceptability of these enriched breads. Added liquid was adjusted for moisture in sweet potato pulp. Sensory evaluation data was analyzed by analysis of variance to determine the major effective factor on the acceptability of bread. Objective measurements were used to determine bread volume, percent crude nitrogen, percent ether extractable crude fat, and moisture content of the breads. The sensory evaluation results indicated that the bread quality decreased with increase in the amount of mung bean flour; mashed sweet potato pulp did not affect the bread quality except that the highest level (30 percent) has a slight adverse effect on bread texture. The characteristics of all breads made with mashed sweet potato pulp and mung bean flour varied with the amount of substitute ingredient. In general, mung bean flour affected the acceptability of bread and bread quality. Breads made with 20 percent mashed sweet potato pulp and 4 or 8 percent mung bean flour were judged to have more desirable eating quality. Volume of bread was decreased with increase in the amount of mung bean flour and of mashed sweet potato pulp. The percent crude nitrogen increased with the addition of mung bean flour and decreased with the addition of mashed sweet potato pulp. Bread enriched with 16 percent mung bean flour and 10 percent mashed sweet potato pulp increased in the amount of percent crude nitrogen approximately 16.7 percent compared with the control. Percent ether extractable crude fat content of all breads was low and was varied. Moisture content increased with addition of mung bean flour and mashed sweet potato pulp. / Master of Science

Since 2015, a U.S. Agency for International Development and Virginia Tech Education and Research in Agriculture collaboration has introduced and tested mung bean as a potential crop to alleviate malnutrition and food insecurity in Senegal. This MS thesis describes a study conducted to assess the impact of mung bean on dietary diversity of Senegalese women and children in the Kaolack, Matam and Bakel localities of Senegal. A mixed-methods research approach included individual surveys to determine dietary diversity scores (DDS) and focus groups to assess the perceived impacts of mung bean. The dietary diversity survey was conducted with 194 participants including adult women, ages 15 to 70 years (n=109) and children, ages 0-10 years (n=85). Half (52%) of the population were mung bean consumers. The dietary diversity surveys revealed an average DDS of 5.73 on a scale of one to 10, with 5.83 and 5.62 for mung bean and non-mung bean consuming groups, respectively. There was a statistically significant difference in DDS between mung-bean consuming women and both mung bean and non-mung bean children, and between mung bean and non-mung bean consumers in Bakel; however, there was no significant difference between overall mung bean and non-mung bean groups DDS. Focus groups (n=11) with mung bean consuming women identified perceived agricultural, health, and financial benefits associated with mung bean consumption. These results can increase our understanding of how mung bean may influence policy-relevant issues for the Senegalese population, including agricultural, health and financial outcomes that are not reflected in dietary diversity surveys. / Master of Science

<p>The action of Mung Bean Nuclease (MBN) on DNA makes it possible to clone intact gene fragments from genes of the malaria parasite, Plasmodium. This &ldquo / genease&rdquo / activity has provided a foundation for further investigation of the coding elements of the Plasmodium genome. MBN has been reported to cleave genomic DNA of Plasmodium preferentially at positions before and after genes, but not within gene coding regions. This mechanism has overcome the difficulty encountered in obtaining genes with low expression levels because the cleavage mechanism of the enzyme yields sequences of genes from genomic DNA rather than mRNA. However, as potentially useful as MBN may be, evidence to support its genease activity comes from analysis of a limited number of genes. It is not clear whether this mechanism is specific to certain genes or species of Plasmodia or whether it is a general cleavage mechanism for Plasmodium DNA .There have also been some projects (Nomura et al., 2001 / van Lin, Janse, and Waters, 2000) which have identified MBN generated fragments which contain fragments of genes with both introns and exons, rather than the intact genes expected from MBN-digestion of genomic DNA, which raises concerns about the efficiency of the MBN mechanism in generating complete genes.</p> <p><br /> Using a large-scale, whole genome mapping approach, 7242 MBN generated genome survey sequences (GSSs) have been mapped to determine their position relative to coding sequences within the complete genome sequences of the human malaria parasite Plasmodium falciparum and the incomplete genome of a rodent malaria parasite Plasmodium berghei. The location of MBN cleavage sites was determined with respect to coding regions in orthologous genes, non-coding /intergenic regions and exon-intron boundaries in these two species of Plasmodium. The survey illustrates that for P. falciparum 79% of GSSs had at least one terminal mapping within an ortholog coding sequence and 85% of GSSs which overlapped coding sequence boundaries mapped within 50 bp of the start or end of the gene. Similarly, despite the partial nature of P.berghei genome sequence information, 73% of P.berghei GSSs had at least one terminal mapping within an ortholog coding sequence and 37% of these mapped between 0-50 bp of the start or end of the gene. This indicates that a larger percentage of cleavage sites in both P.falciparum and P.berghei were found proximal to coding regions. Furthermore, 86% of P.falciparum GSSs had at least one terminal mapping within a coding exon and 85% of GSSs which overlapped exon-intron boundaries mapped within 50bp of the exon start and end site. The fact that 11% of GSSs mapped completely to intronic regions, suggests that some introns contain specific cleavage sites sensitive to cleavage and this also indicates that MBN cleavage of Plasmodium DNA does not always yield complete exons.</p> <p><br /> Finally, the results presented herein were obtained from analysis of several thousand Plasmodium genes which have different coding sequences, in different locations on individual chromosomes/contigs in two different species of Plasmodium. Therefore it appears that the MBN mechanism is neither species specific nor is it limited to specific genes.</p>

The development of a reduced energy Thai style fresh pork sausage has been achieved by replacing fat with carbohydrate and water. Replacing fat in the processed meat formulation with other ingredients is known to alter the product qualities. The addition of carbohydrate, namely mung bean vermicelli, as a fat replacement is suggested to improve the water holding capacity, cooking yield and textural properties as well as reduce the fat and total energy content. This research developed a standard processing methodology for a characteristic Thai fresh pork sausage. The research evaluated the potential of mung bean vermicelli in the formulation of a reduced energy Thai style fresh pork sausage. The reduced energy sausage was developed by replacing fat with 25%, 50%, 75%, and 100% soaked mung bean vermicelli. Quality characteristics of the sausages (chemical, textural and sensory) were evaluated and compared with control sausages (20% fat). The results of the present study indicated that soaked mung bean vermicelli offset many of the characteristic changes usually brought about by fat reduction. The sausages had a decreased cooking loss and an improved yield as well as a reduction in energy content. In addition, they maintained the textural and sensory characteristics of the control Thai style fresh pork sausages. / Master of Science (Hons)

<p>The action of Mung Bean Nuclease (MBN) on DNA makes it possible to clone intact gene fragments from genes of the malaria parasite, Plasmodium. This &ldquo / genease&rdquo / activity has provided a foundation for further investigation of the coding elements of the Plasmodium genome. MBN has been reported to cleave genomic DNA of Plasmodium preferentially at positions before and after genes, but not within gene coding regions. This mechanism has overcome the difficulty encountered in obtaining genes with low expression levels because the cleavage mechanism of the enzyme yields sequences of genes from genomic DNA rather than mRNA. However, as potentially useful as MBN may be, evidence to support its genease activity comes from analysis of a limited number of genes. It is not clear whether this mechanism is specific to certain genes or species of Plasmodia or whether it is a general cleavage mechanism for Plasmodium DNA .There have also been some projects (Nomura et al., 2001 / van Lin, Janse, and Waters, 2000) which have identified MBN generated fragments which contain fragments of genes with both introns and exons, rather than the intact genes expected from MBN-digestion of genomic DNA, which raises concerns about the efficiency of the MBN mechanism in generating complete genes.</p> <p><br /> Using a large-scale, whole genome mapping approach, 7242 MBN generated genome survey sequences (GSSs) have been mapped to determine their position relative to coding sequences within the complete genome sequences of the human malaria parasite Plasmodium falciparum and the incomplete genome of a rodent malaria parasite Plasmodium berghei. The location of MBN cleavage sites was determined with respect to coding regions in orthologous genes, non-coding /intergenic regions and exon-intron boundaries in these two species of Plasmodium. The survey illustrates that for P. falciparum 79% of GSSs had at least one terminal mapping within an ortholog coding sequence and 85% of GSSs which overlapped coding sequence boundaries mapped within 50 bp of the start or end of the gene. Similarly, despite the partial nature of P.berghei genome sequence information, 73% of P.berghei GSSs had at least one terminal mapping within an ortholog coding sequence and 37% of these mapped between 0-50 bp of the start or end of the gene. This indicates that a larger percentage of cleavage sites in both P.falciparum and P.berghei were found proximal to coding regions. Furthermore, 86% of P.falciparum GSSs had at least one terminal mapping within a coding exon and 85% of GSSs which overlapped exon-intron boundaries mapped within 50bp of the exon start and end site. The fact that 11% of GSSs mapped completely to intronic regions, suggests that some introns contain specific cleavage sites sensitive to cleavage and this also indicates that MBN cleavage of Plasmodium DNA does not always yield complete exons.</p> <p><br /> Finally, the results presented herein were obtained from analysis of several thousand Plasmodium genes which have different coding sequences, in different locations on individual chromosomes/contigs in two different species of Plasmodium. Therefore it appears that the MBN mechanism is neither species specific nor is it limited to specific genes.</p>