Investigou-se os efeitos da L-glutamina (1g/kg de massa corpórea) em camundongos jejuados por 24 horas. A L-glutamina atenuou a perda de massa muscular e a diminuição da área das fibras musculares esqueléticas causadas pelo jejum via Akt-mTOR. No sóleo, a L-glutamina estimulou a Akt (início da via), e no EDL ativou a S6 (final da via). Investigou-se também os efeitos da L-glutamina em miotubos (C2C12) cultivados por 48 horas. A diminuição de L-glutamina no meio (de 2 para zero mM) causou balanço proteico negativo e aumento do conteúdo dos aminoácidos livres, exceto dos produtos da glutaminólise, indicando estimulação de proteólise. O aumento de L-glutamina no meio (de 2 para 8 e 16 mM) não alterou o conteúdo intracelular de proteínas e dos aminoácidos livres. Na presença de 2 mM de glutamina no meio, a insulina teve efeito positivo no balanço proteico via Akt/mTOR/S6K, estimulando a S6K. Na ausência de glutamina, houve maior fosforilação de eIF2α estimulada por dexametasona e portanto menor síntese proteica. / We investigated the effects of L-glutamine (1g/kg of body mass) on 24 h fasted mice. L-Glutamine attenuated the loss of muscle mass and the reduction of the skeletal muscle fibers area caused by fasting. This attenuation occurred via Akt-mTOR, however, glutamine stimulated Akt (upstream) in the soleus, whereas it activated S6 (dowstream) in EDL. The effects of L-glutamine on myotubes (C2C12) cultured for 48 hours were also examined. The reduction of L-glutamine in the medium (from 2 to zero mM) decreased protein content and increased contents of all amino acids, except products of glutaminolysis, indicating stimulation of proteolysis. The increased L-glutamine levels in the medium (from 2 to 8 and 16 mM) did not change the intracellular contents of protein and free amino acids. In the presence of 2 mM glutamine, insulin had a positive effect on total protein content through Akt/mTOR/S6K pathway, stimulating S6K. In turn, in the absence of L-glutamine, there was increased eIF2α phosphorylation stimulated by dexamethasone and thus less protein synthesis.

In the light of the facts given in literature it is evident that players in the game of netball need to meet specific anthropometric (body length, body mass, percentage body fat and percentage muscle mass), physical (pliancy, abdominal power, aerobic endurance and anaerobic endurance) and motor (speed over 5 m and 10 m, agility and explosive power) requirements. This consequently necessitates specific attention to be given to the mentioned requirements. In spite of the fact that a few studies do exist that enter into the requirements of the profile of netball players in different positions, as well as into what the effect of a periodization programme is on anthropometric, physical and motor requirements, voids do exist regarding the positional profile of 15 to 17 year old netball players and as to what the effect is of a sport specific periodization programme in the course of a season on 15 to 17 year old netball players. In the light of the above–mentioned, this study was undertaken with the aim to: 1) Determine the requirements for 15 to 17 year old netball players; 2) Compile positional profile scales of netball specific requirement for 15 to 17 year old players in the Tlokwe region; 3) Establish the effect of a sport specific periodization programme on anthropometric, physical and motor requirements for 15 to 17 year old female netball players, of a specific school in the Tlokwe region, in the course of a season. 96 players (28 goalkeepers, 44 centre court players and 24 defenders) between ages 15 and 17 years from two high schools in the North–West Province were used in the study to determine positional differences. A group of only 22 player of one school was exposed to a sport specific periodization programme for purposes of the study, since the coach and players from only one school’s teams were prepared to participate in the sport scientific intervention programme for the full duration of the netball season. The afore–mentioned group was evaluated over a period of two years, namely prior to the start of the season (T1), after conclusion of the season once the sport specific periodization programme was completed (T2), prior to the start of the season in the subsequent year (T3) and after conclusion of the season without the sport specific being followed. The data is processed on the basis of descriptive statistics. Furthermore, the practical significance of test result changes between the respective groups and different test sessions were compared using Cohen’s effect size. Literature was consulted to determine whether specific requirements exist for netball players and whether differences occur in the three positional groups. However, no literature could be traced in which only netball specific requirements for 15 to 17 year old players were focused on. The literature did indeed point out clear anthropometric differences between the three positional groups, namely attacking, centre court and defence players in club and elite netball players. The goalkeeper and defence players are, according to literature, considerably taller and heavier than the centre court players. Literature also indicated that differences do indeed occur regarding physical and motor requirements. Centre court players are significantly faster and more agile than the goalkeeper and defenders. However, there were components of which the differences were not prominent concerning the three positional groups. Where a specific positional profile of anthropometric, physical and motor requirements for 15 to 17 year old female netball players was composed from available data of players in the North–West Province, Tlokwe region, the results of the anthropometric requirements indicated that body length showed a large significant difference between the three positional groups, with defenders being the tallest, followed by goalkeepers, and the centre court players being the shortest. Body mass also showed a medium significant difference between goalkeepers and centre court players as well as between centre court players and defence players. Goalkeepers were heavier than centre court players, and defenders also showed a higher body mass than centre court players. The other two variables, namely percentage fat and percentage muscle mass, only showed small practically significant differences, in this group of netball players, between the test sessions. With the physical and motor requirements, results indicated that large practically significant differences occurred in vertical jumping, 5 m speed, 10 m speed and agility between the groups. The general trend observed in the profiles was that the largest significant differences occurred between goalkeepers and defence players on the one hand and centre court players on the other. Vertical jump and speed showed a large significant difference between goalkeepers and centre court players as well as between centre court players and defenders, although the goalkeepers and defenders’ results corresponded considerably. Other large significant differences occurred in the 10 m speed as well as in 505–agility to the left between centre court players and defenders. In summary it can be mentioned that the most and the largest significant differences occurred between goalkeepers and centre court players as well as between defenders and centre court players. Only one medium significant difference occurred between the goalkeepers and defenders, which is ankle dorsiflexion and which can be ascribed to injuries. From this it can be inferred that a positional profile can indeed be compiled for the different positional groups in netball, but that the requirements of positional variables between goalkeepers and defenders correspond largely and that the large difference between the last–mentioned two groups occur when compared with those of the centre court players of this specific group. The results of the group of twenty–two players that were evaluated twice during the course of both netball seasons indicate that the variable that showed a large significant difference between T1 and T2, following the sport specific periodization program, was body mass (inverted difference). Although there was no large significant difference, it can clearly be deduced from the graphs presented in the study that a visible difference (improvement) was observed in most of the variables. The variables that showed a large practically significant difference with the training of the coach’s general programme, were ankle dorsiflexion on the left, abdominal power and 5 m speed (inverted effect). A number of variables indeed existed that also showed medium and small significant differences during the course of both seasons, but it will not be mentioned here. A number of shortcomings and recommendations did indeed come to the fore during and after the course of the study. It should, however, be borne in mind that such a structured periodization programme is very important for the development of netball potential. / Thesis (Ph.D. (Human Movement Science))--North-West University, Potchefstroom Campus, 2011.

In the light of the facts given in literature it is evident that players in the game of netball need to meet specific anthropometric (body length, body mass, percentage body fat and percentage muscle mass), physical (pliancy, abdominal power, aerobic endurance and anaerobic endurance) and motor (speed over 5 m and 10 m, agility and explosive power) requirements. This consequently necessitates specific attention to be given to the mentioned requirements. In spite of the fact that a few studies do exist that enter into the requirements of the profile of netball players in different positions, as well as into what the effect of a periodization programme is on anthropometric, physical and motor requirements, voids do exist regarding the positional profile of 15 to 17 year old netball players and as to what the effect is of a sport specific periodization programme in the course of a season on 15 to 17 year old netball players. In the light of the above–mentioned, this study was undertaken with the aim to: 1) Determine the requirements for 15 to 17 year old netball players; 2) Compile positional profile scales of netball specific requirement for 15 to 17 year old players in the Tlokwe region; 3) Establish the effect of a sport specific periodization programme on anthropometric, physical and motor requirements for 15 to 17 year old female netball players, of a specific school in the Tlokwe region, in the course of a season. 96 players (28 goalkeepers, 44 centre court players and 24 defenders) between ages 15 and 17 years from two high schools in the North–West Province were used in the study to determine positional differences. A group of only 22 player of one school was exposed to a sport specific periodization programme for purposes of the study, since the coach and players from only one school’s teams were prepared to participate in the sport scientific intervention programme for the full duration of the netball season. The afore–mentioned group was evaluated over a period of two years, namely prior to the start of the season (T1), after conclusion of the season once the sport specific periodization programme was completed (T2), prior to the start of the season in the subsequent year (T3) and after conclusion of the season without the sport specific being followed. The data is processed on the basis of descriptive statistics. Furthermore, the practical significance of test result changes between the respective groups and different test sessions were compared using Cohen’s effect size. Literature was consulted to determine whether specific requirements exist for netball players and whether differences occur in the three positional groups. However, no literature could be traced in which only netball specific requirements for 15 to 17 year old players were focused on. The literature did indeed point out clear anthropometric differences between the three positional groups, namely attacking, centre court and defence players in club and elite netball players. The goalkeeper and defence players are, according to literature, considerably taller and heavier than the centre court players. Literature also indicated that differences do indeed occur regarding physical and motor requirements. Centre court players are significantly faster and more agile than the goalkeeper and defenders. However, there were components of which the differences were not prominent concerning the three positional groups. Where a specific positional profile of anthropometric, physical and motor requirements for 15 to 17 year old female netball players was composed from available data of players in the North–West Province, Tlokwe region, the results of the anthropometric requirements indicated that body length showed a large significant difference between the three positional groups, with defenders being the tallest, followed by goalkeepers, and the centre court players being the shortest. Body mass also showed a medium significant difference between goalkeepers and centre court players as well as between centre court players and defence players. Goalkeepers were heavier than centre court players, and defenders also showed a higher body mass than centre court players. The other two variables, namely percentage fat and percentage muscle mass, only showed small practically significant differences, in this group of netball players, between the test sessions. With the physical and motor requirements, results indicated that large practically significant differences occurred in vertical jumping, 5 m speed, 10 m speed and agility between the groups. The general trend observed in the profiles was that the largest significant differences occurred between goalkeepers and defence players on the one hand and centre court players on the other. Vertical jump and speed showed a large significant difference between goalkeepers and centre court players as well as between centre court players and defenders, although the goalkeepers and defenders’ results corresponded considerably. Other large significant differences occurred in the 10 m speed as well as in 505–agility to the left between centre court players and defenders. In summary it can be mentioned that the most and the largest significant differences occurred between goalkeepers and centre court players as well as between defenders and centre court players. Only one medium significant difference occurred between the goalkeepers and defenders, which is ankle dorsiflexion and which can be ascribed to injuries. From this it can be inferred that a positional profile can indeed be compiled for the different positional groups in netball, but that the requirements of positional variables between goalkeepers and defenders correspond largely and that the large difference between the last–mentioned two groups occur when compared with those of the centre court players of this specific group. The results of the group of twenty–two players that were evaluated twice during the course of both netball seasons indicate that the variable that showed a large significant difference between T1 and T2, following the sport specific periodization program, was body mass (inverted difference). Although there was no large significant difference, it can clearly be deduced from the graphs presented in the study that a visible difference (improvement) was observed in most of the variables. The variables that showed a large practically significant difference with the training of the coach’s general programme, were ankle dorsiflexion on the left, abdominal power and 5 m speed (inverted effect). A number of variables indeed existed that also showed medium and small significant differences during the course of both seasons, but it will not be mentioned here. A number of shortcomings and recommendations did indeed come to the fore during and after the course of the study. It should, however, be borne in mind that such a structured periodization programme is very important for the development of netball potential. / Thesis (Ph.D. (Human Movement Science))--North-West University, Potchefstroom Campus, 2011.

Title: Influence of nutrition stereotyps on body composition - body weight, body fat, muscle mass and body water. Objectives: The aim of this work is to influence changes in body composition in women of various ages, body weight, occupation and movement regime. By adjusting eating habits reduce the amount of body fat while maintaining muscle mass. Methods Basic dietary recommendations for the ratio of macro-nutrients and time aspects of food intake according to the general body weight reduction recommendation. The bioimpedance method is used for assessment of body composition and selected circuits are evaluated at the same time. Based on this data, an individual diet plan was developed for each woman and the dietary effect was continuously evaluated. The study involved six women. Four overweight and obesity weighing 92 ± 17 kg and BMI 35 ± 4 kg/ . Two with a normal weight of 60 ± 2 kg and a BMI of 22.5 ± 0.5 kg/ Average age of women 40 ± 15 years. Results: After the individual diet was applied for three months, we found significant changes in women with higher body weight. They reduced body weight by 6 ± 1 kg and body fat by 5 ± 1 kg. In addition, these persons reduced the waist circumference by 5 ± 2 cm and the hips by 4 ± 2 cm. For the remaining two subjects with normal body weight, we did not...

The aim of the diploma thesis was to determine, which nutritional supplements are selected by athletes of strength and endurance sports branches and what is the reason for their option. The theoretical part characterizes the 8 selected sports sector from point of view of the sport performance structure. There is also mentioned the issue of nutritional supplements, their division, legislation or general recommendations for the use in sport. The last chapter deals with the requirements for the use of nutritional supplements in different periods with respect to the branche of sport. Research part has specifically determined the nutritional strategy for athletes in the field of mentioned food supplements at strength and endurance-oriented sports disciplines by using the questionnaire. The research is supported by an interview with an expert nutritionist and sports through collaboration with the staff of the Centre for Sports of the Ministry of Interior

Hematooncological diseases are often accompanied by dietary restriction, especially in cytotoxic therapy. The main purpose of the work was to assess the effect of high-dose chemotherapy on the change of nutritional status in two groups of hematooncological patients. A total of 16 patients were enrolled. Changes of the body composition were evaluated using bioelectrical impedance analysis supplemented by monitoring of biochemical nutritional indicators. Observations showed that in both groups the majority lost weight. In the first group of eight patients with acute myeloid leukemia observed during three consecutive hospitalizations, the median of change of body weight was -3.7 kg (-4.3%). Loss of lean body mass with a median value of -4.8 kg (-7.2%) was detected at all patients. Body fat was reduced at half of the patients. In some cases, with length of observation, there was an increase in fat mass along with visceral fat. In the second group, which included eight patients (after autologous hematopoietic stem cell transplantation) at whom one hospitalization was evaluated, body weight was reduced at six patients. The medianof change of body weight was -2.1 kg (-2.3%). At five patients, the treatment representeda loss of active metabolic mass. The change of the weight of the lean body mass was shown...

Title: Influence of nutrition stereotypes on body fat reduction Objectives: The aim of this work is to achieve a reduction in body fat through appropriate eating habits. Acquired theoretical knowledge applied to practice for six women of different age and movement regime. Inform about the procedures of this intervention. Verify the validity of theoretical knowledge in practice. Achieve the desired results. Methods Basic dietary recommendations, peer-to-peer comparisons and time aspects of food intake. Furthermore, bioimpedance measurements on the In Body 230 instrument and physical body measurements by a tape method to determine the baseline status of the examined women and to subsequently monitor their running state at specified intervals. Developing a diet for each of these women and evaluating the on-going status. Results: The result of this diploma thesis is to make changes in eating habits that will lead to the reduction of body fat. Partial results are the protocols from the continuous measurement on the In Body 230 at the Lunacor s.r.o. In Kroměříž and fitness center Fit2b s.r.o. In Prague 8 - Bohnice.. Key words: Nutrition, way of living, lifestyle, obesity, BMI, dietary, basal metabolism, nutrition ratio, nutritional habits, reduction, body weight, body fat, muscle mass, body water,...