Thesis (M. Phil.)--University of Hong Kong, 2008. / Includes bibliographical references (p. 117-147) Also available in print.

Thesis (Ph. D.)--University of Washington, 2004. / Vita. Includes bibliographical references (leaves 113-117).

Thesis (M. Med. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 41-49).

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2009. / Härtill 5 uppsatser.

Thesis (M.S.)--Rutgers University, 2008. / "Graduate Program in Chemistry and Chemical Biology." Includes bibliographical references (p. 23-24).

Thesis (M.Med.Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 41-49). Also available in print.

Mestre -- Escola Nacional de Saude Publica, Rio de Janeiro, 1999.

Thesis (Ph. D.)--University of Washington, 2006. / Vita. Includes bibliographical references (leaves 151-169).

Made available in DSpace on 2014-08-13T14:50:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-11-18Bitstream added on 2014-08-13T18:01:11Z : No. of bitstreams: 1 000736114_20141218.pdf: 167852 bytes, checksum: 195f78eda97184540a4c8fa710ee18f9 (MD5) Bitstreams deleted on 2014-12-19T18:32:47Z: 000736114_20141218.pdf,Bitstream added on 2014-12-19T18:33:34Z : No. of bitstreams: 1 000736114.pdf: 2142445 bytes, checksum: 4c37b6a0b38133d350c91b6a9b7b59cc (MD5) / A tuberculose (TB) é uma doença infecciosa crônica causada principalmente pelo bacilo aeróbico Mycobacterium tuberculosis. Ela provoca problemas de saúde entre milhões de pessoas a cada ano, sendo classificada como a segunda causa de morte por uma doença infecciosa em todo o mundo. Assim, o objetivo desse estudo foi analisar a epidemiologia clássica e molecular da tuberculose pulmonar, em pacientes do Norte de Minas Gerais. Foi realizado um levantamento epidemiológico, de caráter observacional, longitudinal, retrospectivo e de delineamento quantitativo. A população foi composta por pacientes com suspeita de TB pulmonar, residentes na Região Norte de Minas Gerais, atendidos no Hospital Universitário Clemente de Faria (HUCF) da Universidade Estadual de Montes Claros (UNIMONTES), no período de janeiro de 2011 a dezembro 2012. As amostras clínicas (escarro ou lavado broncoalveolar) destes pacientes foram analisadas, através da técnica de Ziehl- Neelsen e da cultura pela método de Ogawa-Kudoh. Os isolados clínicos obtidos foram submetidos a identificação molecular, a genotipagem pelas técnicas de Spoligotyping, RDRio, ERIC-PCR e MIRU-VNTR e ao teste de susceptibilidade a fármacos. A correlação epidemiológica clássica e molecular foi realizada através dos dados da genotipagem e dos dados epidemiológicos clássicos. Dos 345 pacientes avaliados, 33 (9,6%) apresentavam a doença, sendo 69,7% residentes em Montes Claros e 30,3% em outras cidades do Norte de Minas Gerais. Houve predomínio da TB em indivíduos do sexo masculino (75,8%), da etnia parda (60,6%), de baixa escolaridade (42,4%) e em idade economicamente ativa (78,9%). A maior parte dos casos eram novos (69,7%) e a baciloscopia foi positiva em 75,8% dos pacientes. A taxa de cura foi baixa (30,3%) e a de transferência elevada (30,3%). Os agravos mais evidentes continuam sendo, a co-infecção TB/HIV (6,1%) e o alcoolismo (24,25%). A análise dos 33 isolados clínicos ... / Tuberculosis is a chronic infectious disease caused by the bacillus aerobic Mycobacterium tuberculosis. It causes health problems among millions of people each year, being ranked as the second leading cause of death from infectious disease worldwide. Thus, the aim of this study was to analyze the epidemiology of classical and molecular pulmonary tuberculosis in patients in North of Minas Gerais. We conducted an epidemiological study, an observational, longitudinal, retrospective and quantitative design. The population consisted of patients with suspected pulmonary TB, residents in the north of Minas Gerais patients at the University Hospital Clemente de Faria (HUCF), State University of Montes Claros (UNIMONTES), from January 2011 to December 2012. The clinical samples (sputum or bronchoalveolar lavage) of these patients were analyzed by Ziehl-Neelsen staining and culture by the method of Ogawa-Kudoh. Clinical isolates were subjected to molecular identification, genotyping by Spoligotyping techniques, RDRio, ERIC-PCR and MIRU-VNTR and drug susceptibility testing. The classical and molecular epidemiological correlation was performed using data from genotyping and classical epidemiological data. Of the 345 patients evaluated, 33 (9.6%) had the disease, with 69.7 % living in Montes Claros and 30.3 % in other cities in northern Minas Gerais. There was a prevalence of TB in males (75.8%), of mulattoes (60.6%), low education (42.4 %) and working age (78.9%) . The majority of new cases were (69.7%) and the smear was positive in 75.8 % of patients. The cure rate was low (30.3%) and high transfer (30.3 %). The most obvious grievances remain, the co -infection TB / HIV (6.1%) and alcoholism (24.25%) . Analysis of 33 clinical isolates by Spoligotyping allowed the identification of 22 different genetic profiles (66.7% genetic diversity). Among the genetic profiles found 15 (45.5%) LAM, 04 (18.2 %) belong to T family, 01 families (3.0%) to Haarlen ...

Orientador: Fernando Rogério Pavan / Banca: Mario Hiroyuki Hirata / Banca:Rosilene Fressatti Cardoso / Banca: Jean Leandro dos Santos / Banca: Carla Raquel Fontana / Resumo: A busca por novos fármacos faz-se necessária sob dois principais pontos: a cura/tratamento do paciente pela melhora de qualidade de vida e a importância de tratalo a fim de eliminar focos de propagação das doenças infecto-contagiosas. A tuberculose (TB) se insere neste panorama, visto que é uma doença infecciosa que tem como principal patógeno o Mycobacterium tuberculosis (Mtb). A taxa de mortalidade da TB caiu 45% desde 1990 e o mundo estaria a caminho de atingir a meta global de redução de 50% até o ano de 2015. A última pesquisa conduzida no ano de 2015 mostrou 6,3 milhões de casos de TB notificados pelos programas nacionais da doença, dos quais 55% correspondem a casos de co-infecção TB-HIV. Uma recente problemática repercute: a resistência. Fato comprovado pela estimativa de 580.000 novos casos de TB-MDR no ano de 2015 comparados aos 480.000 casos registrados em 2014; e o relato de 9,7% desses casos serem na verdade TB-XDR. Baseado nesses dados epidemiológicos observamos a necessidade da busca de novos fármacos a fim de melhorar a terapia e consequentemente a qualidade de vida e cura dos pacientes. Nesse sentido, essa tese buscou investigar o potencial biológico de vinte e dois novos compostos n-óxidos: derivados furoxânicos, benzofuroxânicos e derivados quinoxalínicos; in vitro e in vivo. Investigamos também a segurança e dados preliminares ao mecanismo de ação. Os compostos demonstraram atividade (CIM90) entre 0,40 - 62 µM. Dentre todos os compostos n-óxidos estudados,... (Resumo completo, clicar acesso eletrônico abaixo) / Abtract: The search for new drugs is necessary under two main points: the cure / treatment of the patient for the improvement of quality of life and the importance of treating it in order to eliminate outbreaks of the spread of infecto-contagious diseases. Tuberculosis (TB) is part of this scenario, since TB is an infectious disease with Mycobacterium tuberculosis (Mtb) as its main pathogen. The TB mortality rate has dropped by 45% since 1990 and the world is on track to meet the global 50% reduction target by 2015. The latest survey conducted in 2015 showed 6.3 million TB cases notified by national disease programs, of which 55% correspond to cases of HIV-TB co-infection. A recent problem has repercussions: resistance. This is proven by the estimated 580,000 new cases of MDRTB in 2015 compared to 480,000 cases in 2014; actually 9.7% of these cases are TBXDR. Based on these epidemiological data, we observed the need to search for new drugs in order to improve therapy and consequently the quality of life and cure of patients. In this sense, this thesis sought to investigate the biological potential in vitro and in vivo of twenty-two new n-oxide compounds: furoxanic, benzofuroxanic and quinoxaline derivatives. We also investigated the safety and preliminary data on the mechanism of action of these compounds. The compounds showed activity (MIC90) in a ranging 0.40 to 62 µM. Among all the n-oxide studied, the benzofuroxan (8) was more promising (MIC90 values of 1.10 and 6.62 μM against the active and latent bacteria, respectively; and cytotoxicity against the MRC-5 cell line (IC50) was obtained at the concentration of 519.2 μM). This compound also showed activity against the monoresistant strains of important drugs of the therapy (isoniazid, rifampicin, moxifloxacin, bedaquiline, cycloserine and streptomycin) as well as... (Complete abstract click electronic access below) / Doutor