In my dissertation "Problémy trestního soudnictví ve věcech mládeže" I speak about the issues of criminality of the young and about the forms of reactions to misdemeanour caused by the young perpetrators or children. The main legal regulation in this area is the law no. 218/2003, about the judiciary in cases of the young, that contains the arrangement of the material and the procedural law. In connection to the criminal law and the penal code it is a lex specialis. In the first chapter I speak about the theoretical fundation of the law arrangement, and so about the conception of the restorative judiciary. The main idea of this conception is to favor the educational action before repression. The aim is the relationship reconsiliation between the perpetrator and the victim, the awareness of one's deeds and subsequent continuance in one's life in conformity with legal order. The theme of the second chapter is the terminology of the judiciary law in cases of the young. A different terminology was implemented in order to reduce the stigmatization of the young perpetrators, due the interest of their proper development. The third chapter represents a brief retrospect to the history of law arrangement in the area of criminality of the young. In particular, it speaks about the judiciary law among the young...

Anglický abstrakt The issues of the juvenile criminal justice This thesis is focused on question of juvenile delinquency, committing of unlawful acts of the youth and the children younger fifteen years old and penalties which are imposed according to Act No. 218/2003 Coll. This thesis deals with questions connected with delinquency of juvenile and handle them from view of substantial law, as to approach to them from criminality and criminology views. Thesis is focused on introducing united perspective of chosen topics juvenile criminal justice and in case of options settling alternative solutions. In the end of each chapter the author's own opinion is presented and in the case of problematic regulation also possible solution is proposed. Test is divided in to five chapters and further split into subsections. In first chapter basic key concepts regarding main thesis are defined. In the second chapter thesis deals with juvenile delinquency, especially about cause leading to delinquent acting of individuals and main features of unlawful acts committed by the youth. Further there are summarised data from official statistics. Third chapter is focused on still actual topic of legal liability of the juvenile, especially on setting age limit of legal liability and intellectual and moral maturity and with this...

Stopped-flow experiments in which the NAD-malic enzyme was preincubated with different reactants at near saturating substrate concentrations suggest a slow isomerization of the E:NAD:Mg complex. The lag is eliminated by preincubation with Mg˙² and malate suggesting that the formation of E:Mg:Malate either bypasses or speeds up the slow isomerization step. Circular dichroic spectral studies of the secondary structural changes of the native enzyme in the presence and absence of substrates supports the existence of conformational changes with NAD˙ and malate. Thus, a slow conformational change of the E:NAD:Mg complex is likely one of the rate-limiting steps in the pre-steady state.

>Magister Scientiae - MSc / A metagenomic gene library prepared from soil found beneath a mummified seal carcass in the Miers Valley, Antarctica, suggests an environment rich in uncharacterised biodiversity including enzymes with possible application to industrial processes. A sequence based gene mining investigation was performed on a clone, which archives a metagenomic sequence from this environment. The sequence was annotated using de novo bioinformatics and molecular biology techniques. A predicted NAD+-dependent DNA ligase, ligDB1 was selected for further characterisation. LigDB1 encodes a gene product that contains all the sequence features of a functional ligase. The protein was overexpressed in a heterologous E. coli host and purified to homogeneity. LigDB1 did not exhibit nick sealing activity, but was able to perform AMP-dependent DNA relaxation in the presence of high concentrations of enzyme. DNA modifying enzymes from cold environments perform optimally at low temperatures and may be of use as molecular tools in biotechnology. Complete characterisation of this enzyme is subject to further investigations

The adipose tissue lipid chaperones aP2 and mal1, also known as fatty acid binding proteins (FABPs), are significant molecules contributing to metabolic homeostasis, whereby their absence promotes physiological changes that improve systemic metabolism. Identification of palmitoleate as a lipokine generated in aP2-mal1 deficiency--originating from adipose and directing the lipogenic program in liver, established a role for these chaperones in linking adipocyte and hepatic function. We have recently demonstrated a functional role for secreted aP2 in the activation of gluconeogenesis and hepatic glucose output, further designating this molecule as an adipocyte-derived regulatory factor that influences liver metabolism. Key molecules linking the metabolism of nutrients in energy generating pathways are the nucleotide cofactors NAD and NADH. Together, these molecules function to coordinate the maintenance of redox reactions during normal cellular metabolism and act as required substrates for enzymes such as sirtuins and poly ADP-ribose polymerases. Using global metabolite profiling, we show that combined deficiency of the adipose tissue lipid chaperones aP2 and mal1 leads to a hepatic nucleotide imbalance resulting from metabolic reprogramming in liver. We demonstrate that this reprogramming of metabolite flux is accompanied by significant alterations in liver NAD metabolism and establish a role for aP2 in directing substrate utilization through inhibition of the rate-limiting enzyme for NAD synthesis, nicotinamide phosphoribosyltransferase. Several models for the proposed regulatory pathways that link nutrient metabolism to aging include mechanisms that are NAD dependent. Accordingly, we found that long-term FABP deficiency confers a strong resistance to aging related metabolic deterioration. Together, the findings presented in this thesis support a considerable role for FABPs in the regulation of NAD metabolism and healthspan.

Heterologous expression systems such as COS-7 cells have demonstrated the profound effects of KCNAB1-3 or Kvβ1-3 proteins on voltage gated potassium channels (Kv) channels. Indeed, in the presence of these β-subunits transiently expressed Kv channels are often modulated in multiple ways. Kv channel membrane expression is often increased in the presence of β-subunits. In addition, non-inactivating Kv currents suddenly become fast-inactivating and fast-inactivating channels become even faster. While much research has demonstrated the profound effects the β-subunits in particular the Kvβ1 subunit have on transiently expressed Kv currents little to date is known of the physiological role it may play. One study demonstrated that by “knocking out” Kvβ1 cardiomyocyte current changes were noted including a decrease in the Ito,f current. While this novel finding demonstrated a key cardiac physiological role of the Kvβ1 subunit it left many unanswered questions as to determine the cardiovascular regulation the Kvβ1 subunit provides. Indeed, cardiac arrhythmias and other electrical abnormalities within the heart such as long QT present patients with many unfortunate unknowns. Many of these incidences occur often abruptly with cardiac electrical abnormalities. Genetic research has begun to shine light on key cardiovascular genes in particular those coding for ion channels and auxiliary subunits or β-subunits. Kv channels and their β-subunits have gained particular notoriety in their key responsibility in restoring the resting membrane potential known as the repolarization phase. Indeed genetic manipulation and physiological examination of Kv channels and recently their β-subunits has demonstrated profound physiological results including prolonged QT durations within mice altered functional activity during physiological cycles such as estrus. While initial findings of Kvβ1 have demonstrated profound cellular and cardiomyocyte current alterations much still remains unknown. Therefore, this work hypothesizes that the Kvβ1 subunit provides a profound cardiovascular role in regulation and redox sensing at the physiological and pathophysiological level in both males and females. This work identifies a sex-based difference in cardiovascular regulation by Kvβ1 as well as demonstrated a profound redox sensing ability during altered metabolic states seen in pathophysiological conditions.

The topic of my Master's degree thesis is "The issue of criminal juvenile justice". It is focused on the area of unlawful acts of youth and children under fifteen years old. I chosed this topic because of my interest in this area. The main focus of my thesis is juridical, and minor part is also devoted to criminal aspects. I focus mainly on the sanctions. Thesis is divided into seven chapters and further split into subsections. In the first chapter I describe basic facts of the youth criminal acts. I'm focusing mainly in the characteristic criminal conducts and behaviors. The following chapter is focused on Juvenile criminal justice and mainly Law No. 218/2003 Coll. It deal with the purpose of the law and the basic concepts. The third chapter describe the area of the legal accountability of youth people, it means the question of legal accountability and moral and intellectual maturity. It deals with issue of insanity and age limit for criminal accountability of youth. In the fourth chapter I have focused on the termination of criminal liability, active repentance and the Limitation of the law. The fifth chapter is focused on the analysis of measures and their deficiency. This chapter represents the core part of the thesis. In sixth chapter I deal with the question of specific forms of criminal...

The topic of the diploma thesis is the impact of the St. Wenceslas'cult to the tourism in Brandýs nad Labem -- Stará Boleslav. First part of the thesis consists of basic information about St. Wenceslas and the development of his cult in periods of the Czech history. Second part represents the survey of the Czech nation about St. Wenceslas. Last part of the thesis forms the analysis of the tourism industry in Brandýs nad Labem - Stará Boleslav and the impact of St. Wenceslas cult to the tourism of the town.

The aim of the diploma thesis is to monitor and analyze cyclotourism in the selected region of the Czech Republic. The thesis focuses on The Elbe Trail and its economic benefits for the selected area, which is the Ústi region. The theoretical part of the thesis introduces the basic terms related to the cyclotourism, funding and organization of cyclotourism in the Czech Republic. The practical part analyzes the Elbe Trail as a tourist product and then evaluates the economic benefits of the Elbe Trail.

The aim of the thesis Impact of rail transport on tourism development in the Czech Republic is the analysis of correlation between rail transport and tourism in the Czech Republic with reference to global trends in this field and proposition of appropriate steps to deeper and more effective cooperation. The first chapter is devoted to definition of rail transport, history of rail transport in the Czech Republic, characterization of the current state of railways in the Czech Republic, position of rail transport on the Czech transport market and use of rail transport in tourism. Second chapter concentrates on analysis of three chosen tourist destinations in terms of the availability of rail. In the last chapter overall situation in this field is analyzed and suggestions for the further development are given.