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Chez les vertébrés, le battement coordonné des cils motiles présents par centaines à la surface apicale des cellules multiciliées (MCC) est requis pour propulser directionnellement les fluides biologiques à l'intérieur de certains organes (voies respiratoires, ventricules cérébraux, trompes utérines ou certaines structures embryonnaires). De nombreuses pathologies humaines sont associées à des défauts ciliaires ou à une perte des MCC (dyskinésies ciliaires, mucoviscidose, asthme,...). Dans ce contexte, mon travail de thèse a consisté à élucider les mécanismes complexes contrôlant la différenciation des MCC et donc la formation des cils motiles (multiciliogenèse). Par des approches de génomiques fonctionnelles à partir de deux modèles d'épithéliums multiciliés évolutivement éloignés (épithélium respiratoire humain et épiderme d'embryon de Xénope) nous avons identifié la famille des microARN (petits ARN non-codants régulateurs de l'expression génique) miR-449 comme majoritairement exprimée dans les MCC. Nous avons montré que miR-449 contrôle la multiciliogenèse i) en bloquant le cycle cellulaire, ii) en réprimant directement la voie de signalisation Notch et iii) en inhibant l'expression de la petite GTPase R-Ras. Enfin, nos travaux montrent que l'ensemble de ces mécanismes est conservé chez les vertébrés. En conclusion, miR-449 est un nouveau régulateur clé de la multiciliogenèse conservé au cours de l'évolution. Nos résultats pourraient ouvrir la voie à de nouvelles stratégies thérapeutiques utilisant des petits ARN régulateurs dans le traitement de certaines pathologies associées à des défauts ciliaires.

The central properties of stem cells are the pluripotency and the capacity of self-renewal. Hematopoietic stem cells (HSCs) posses such common features that allows them to generate all the cells of the hematopoietic compartments, maintaining in the same time the HSC pool. We develop approaches focused on ex vivo HSC expansion through activation by exogenous HOXB4 (human HSCs) or Notch/Dll-4 ligand (murine HSCs). Two independent transcriptomic analyses surprisingly converged toward an increased expression of two genes never identified sofar as crucial for HSC functions: Pumilio1 (Pum1) and Pumilio2 (Pum2). Pum1 and Pum2 are posttranscriptional regulators belonging to the Pumilio-FBF (PUF) family of RNA-binding proteins. Although it was established that the primordial role of PUF proteins is to sustain mitotic proliferation of stem cells in Invertebrates, so far nothing is known about the role of Pum1 and Pum2 in human and murine HSCs.For these reasons, we have investigated the roles and mechanisms of action of Pum1 and Pum2 in murine and human HSCs through shRNA strategy. Pum1 and Pum2 knockdown (KD) in murine HSCs led to a decreased HSC expansion and clonogenic potential ex vivo, associated with an increased apoptosis and a cell cycle arrest in G0/G1 phase. KD of both Pum1 and Pum2 enhanced these effects, suggesting a cooperative effect. Expansion and clonogenic potential of KD Pum1 HSCs were rescued by enforced expression of Pum1 (insensitive to our shRNA), thus validating the specificity of our shRNA. Enforced expression of Pum1 could not rescue the functions of Pum2 KD HSCs, highlighting the non-redundant role of these proteins. Furthermore, when Pum1 or Pum2 KD HSCs were inoculated into lethally irradiated mice to follow the long-term hematopoietic potential, only rare bone marrow cells derived from Pum1 and Pum2 KD HSCs were evidenced after 4 months, contrary to control HSCs. Identical results were obtained with human Pum1 or Pum2 KD HSCs.In conclusion, our results demonstrate the involvement of Pumilio factors in stemness maintenance, expansion and survival of murine and human HSCs. Identification of Pumilio factors and their targets as new regulators of HSCs expansion will allow consider them as new tools for therapeutic perspectives.

Osteoarthritis (OA) is the leading cause of disability in North American and has major economic consequences for society. People with knee OA experience the worst quality of life, among musculoskeletal conditions, with function and mobility being influenced by symptoms such as pain and stiffness. However, the impact of OA symptoms varies due to intrinsic and extrinsic factors, leading many researchers to employ biopsychosocial and other population health frameworks to study the disease. These population health approaches have not been adopted when studying knee OA outcomes in bioarchaeology, where a limited biological lens prevails due to the sole reliance on skeletal remains. The purpose of this research was to explore methods for identifying the impairment potential of knee OA in archaeological populations using a clinical sample and population health approaches. Clinical studies have the advantage of assessing not only the biological implications of knee OA but also the functional outcomes. By creating a knee OA grading system applicable for both MRI and dry bone femora samples (Clinical Archaeological Osteoarthritis Score) a link between clinical and archaeological populations was proposed. Using this link to infer functional deficits onto archaeological populations using population health frameworks, a theoretical analysis was performed with two populations; the 17th century Huron and the 19th century Inuit from the Igloolik region of Nunavut. The results demonstrated the increased impairment potential of knee OA in the Inuit population versus the Huron population, produced by contrasting factors captured by the determinants of health, including social and physical environments.

For the manufacture of higher strength pipelines to be feasible, a better understanding of the effects of welding on toughness is necessary. Bevel submerged arc welds were performed on X80 grade steel. The subsequent Charpy V-notch (CVN) test results indicated that the notch placement in the various heat affected zone regions, and hence the bead geometry, affected the test results. A series of bead-on-plate (BOP) submerged arc welds then were performed on X70 grade steel plate to determine the effects of current, voltage, heat input, polarity, and waveform manipulation (i.e., balance, offset, and frequency) on both single and tandem weld bead geometry. A new bead profile characteristic, the SP ratio, is proposed to describe weld bead geometry, and its relationship with welding parameters is discussed. Sub-size CVN specimens, pulled from many of the BOP weld coupons, were then tested. The greatest subsize CVN fracture energies were achieved when the bead was produced using lower heat input, and when the bead profile possessed a greater SP ratio. / Materials Engineering

This thesis provides a structural and materials engineering explanation for many of the running fractures that occurred in steel structures during the destructive Kobe and Northridge earthquakes in the mid 1990s. A method is developed that allows the ductile endurance of structural steel members subjected to cyclic plastic deformation during earthquakes to be assessed and for pre-necking running fractures to be avoided. The study commenced following the 2000 World Earthquake Conference in Auckland. The conference brought together the findings of the huge research effort, in America, Japan, Europe and New Zealand, that followed the Kobe and Northridge earthquakes. The running fractures that had occurred in steel structures represented an unpredicted failure mode that structural engineers have not known how to predict or suppress through the engineering design process. A clear fundamental understanding of the causes and how to prevent the fractures did not arise from the conference. In fact apparently conflicting results were reported. Full scale cyclic tests in New Zealand on structural assemblies had not resulted in running fractures, whereas tests in American and Japan had. Structural engineers designing earthquake resistant structures rely on constructional steel to be materially homogeneous and nominally tri-linear in behaviour. Steel is expected to behave elastically under regular in-service loading, have a reliable and flat yield stress-strain characteristic, and under overload then develop predictable levels of strain-hardening in conjunction with significant plastic elongation up to its ultimate tensile strength. Steel is expected to eventually fracture after further plastic elongation and necking. Ductile design strategies and methods utilise the plastic elongation characteristics of steel to protect structures in earthquake. Plastic deformation is considered to beneficially dissipate energy generated in the structure by a severe earthquake and also dampen the structure’s response. The occurrence of running fracture without significant cyclic plastic deformation and before section necking in steelwork, therefore undermines the basis of the ductile seismic design approach. The initial part of the thesis is devoted to bringing together the fundamental aspects of materials engineering related to fracture of constructional steel. This is intended to provide a bridge of knowledge for structural engineering practitioners and researchers not fully conversant with materials engineering aspects of fracture. Fracture behaviour in steel is a broad and complex topic that developed rapidly in the twentieth century driven by the demands of technological growth. The unexpected fracture of welded liberty ships at sea in World War 2; the need for reliable long term containment for the nuclear reactors in the 1950s and 1960s; and prevention of fatigue failures in aircraft frames since the 1950s all drove engineering research into steel fracture behaviour. There are many subtle variations in definitions in the published literature on fracture that can be confusing. Therefore an attempt has been made to clarify terminology. The term brittle fracture in particular is only used in this thesis as applying to running fracture when the general or far field tensile stresses are below the yield stress of the steel. The term pre-necking or running fracture is preferred to describe the condition more broadly which may occur prior to and also after general yielding, but before section necking. Running fracture is a manifestation of pre-necking fracture in which insufficient plastic flow is available in the assembly to absorb the energy released upon fracture. The experimental studies investigated the behaviour of constructional steel commonly used in New Zealand, at various levels of plastic strain. This started with Charpy V-Notch (CVN) testing which revealed that a significant transition temperature shift and curve shape change occurs with increasing plastic strain and the associated strain-hardening. This showed that the ability of steel to avoid pre-necking or running fracture reduces as the level of plastic strain-hardening increases. Temperature controlled Crack Tip Opening Displacement (CTOD) testing was then undertaken. The setting of testing temperatures for the CTOD tests were guided by review of the CVN test results, using published CVN to fracture toughness correlation methods. However running cleavage fractures developed in the CTOD specimens at higher than predicted temperatures of 10 oC and 20 oC. These are typical service temperatures for structures in New Zealand and so are very likely to occur at the time of an earthquake. The implication from this is that there are levels of strain-hardening and conditions of material notching constraint that can lead to pre-necking and running fracture in New Zealand fabricated steel structures, under severe earthquake loading. Care was taken in the CTOD testing to monitor and maximise the capture of data electronically using a specially developed Direct Current Potential Drop method. This allowed the test results to be analysed and considered in varying ways, leading to a consistent assessment of the CTOD, crack growth, and the specific work of fracture in each test piece. While CTOD test results have sometimes been published by structural and welding engineering researchers in the wake of Kobe and Northridge, the results were typically of little use for this study as the CTOD initiation point was generally not identified effectively. The effect of remote plastic flow in the specimens was also not adequately accounted for. The CTOD test results were often simply used to help correlate other factors observed by the researchers. Side-grooving of specimens was not reported as having been used in any of the published results reviewed. When conducting CTOD test with highly ductile constructional steels it is very difficult to get useful CTOD results if the specimens are not side-grooved, as significant necking and tunnelling will otherwise occur and limit the usefulness of the results. Work by Knott and also by McRobie and Smith was seminal in terms of identifying some critical aspects of plane strain development in CTOD tests, and the links to non-metallic particle density with respect to fracture toughness and CTOD at initiation. Some of their findings with regards to the effect of pre-strain on CTOD initiation were subsequently found to confirm the experimental findings in this study. No effective methodology for prediction of pre-necking or running fracture in a structural member or assembly when subjected to gross plastic cyclic deformation was found to exist in the literature. It was concluded however that the principles of specific work of fracture, and monotonic and cyclic fracture similitude were particularly relevant. These were therefore utilised in the development of the design method proposed in this thesis. The CTOD test results were reviewed, isolating the remote plastic flow component, to determine the critical specific work of fracture property Rc of the steels tested. A meeting with Professor Kuwamura at the University of Tokyo was providential, allowing discussion of his similitude principle, and observations in person of some of the fractured specimens developed during his full scale test series’. Running fractures with cleavage were evident in the specimens, with their tell-tale chevron markings. He had predicted running fracture problems in structures in Japan ahead of the Kobe earthquake and been largely ignored. His insights were subsequently seriously considered in Japan after the earthquake. He and his colleagues developed the principle of structural similitude that relates monotonic fracture displacement ductility to cyclic fracture displacement ductility for a particular assembly. This arose from their observation that running fractures developed from ductile crack formation at blunt notches in structures. The similitude principle has echoes of the Coffin-Manson approach to ductile crack initiated low cycle fracture. The principle of similitude has a log–log relationship as does the Manson-Coffin relationship. So where notch plasticity controls the initiation of fracture in a structural assembly it is conceptually reasonable to expect that the number of cycles to initiation of fracture from a notch will have a log–log relationship to the amplitude of the cyclic strain developed in the notch. Kuwamura found that steel assemblies with lower CVN energy had reduced cyclic fracture endurance than the same assemblies made with steel with higher CVN impact energy. However no method of predicting performance of any particular assembly could be developed from his observations. The benefit of his method primarily relates to the minimising of testing necessary to assess the fracture limited cyclic displacement ductility of a structural assembly. However it doesn’t provide a means for designing a structural assembly to achieve specific levels of ductile endurance other than clearly identifying the need to use steel with good CVN characteristics. The most significant development arising from this thesis is therefore the development of a design method to assess cyclic ductile endurance. The method utilises the specific work of fracture properties obtained from CTOD specimens of the steel in conjunction with a relatively simple fracture mechanics assessment and an elasto-plastic finite element analysis (FEA). The FEA model is used to determine the displacement ductility of the assembly at the calculated onset of pre-necking fracture. The elasto-plastic stress–strain properties of the steel in various pre-strain states required for the FEA may be derived from tensile testing. Kuwamura’s similitude principle is then used to predict cyclic plastic endurance at various constant displacement ductility amplitudes. The method is extended using Miner’s rule to allow for the effects of increasing variable amplitude cyclic plastic loading. In summary the thesis explains why pre-necking and running fractures occur in steel members subjected to cyclic plastic deformation during a severe earthquake. In addition a method for consistently assessing the ability of structural steel assemblies to achieve a specified level of ductile endurance during earthquakes is proposed. The method is verified against published results for a cyclic test of a simple steel member with a crack at mid-span. / Whole document restricted, but available by request, use the feedback form to request access.

This thesis provides a structural and materials engineering explanation for many of the running fractures that occurred in steel structures during the destructive Kobe and Northridge earthquakes in the mid 1990s. A method is developed that allows the ductile endurance of structural steel members subjected to cyclic plastic deformation during earthquakes to be assessed and for pre-necking running fractures to be avoided. The study commenced following the 2000 World Earthquake Conference in Auckland. The conference brought together the findings of the huge research effort, in America, Japan, Europe and New Zealand, that followed the Kobe and Northridge earthquakes. The running fractures that had occurred in steel structures represented an unpredicted failure mode that structural engineers have not known how to predict or suppress through the engineering design process. A clear fundamental understanding of the causes and how to prevent the fractures did not arise from the conference. In fact apparently conflicting results were reported. Full scale cyclic tests in New Zealand on structural assemblies had not resulted in running fractures, whereas tests in American and Japan had. Structural engineers designing earthquake resistant structures rely on constructional steel to be materially homogeneous and nominally tri-linear in behaviour. Steel is expected to behave elastically under regular in-service loading, have a reliable and flat yield stress-strain characteristic, and under overload then develop predictable levels of strain-hardening in conjunction with significant plastic elongation up to its ultimate tensile strength. Steel is expected to eventually fracture after further plastic elongation and necking. Ductile design strategies and methods utilise the plastic elongation characteristics of steel to protect structures in earthquake. Plastic deformation is considered to beneficially dissipate energy generated in the structure by a severe earthquake and also dampen the structure’s response. The occurrence of running fracture without significant cyclic plastic deformation and before section necking in steelwork, therefore undermines the basis of the ductile seismic design approach. The initial part of the thesis is devoted to bringing together the fundamental aspects of materials engineering related to fracture of constructional steel. This is intended to provide a bridge of knowledge for structural engineering practitioners and researchers not fully conversant with materials engineering aspects of fracture. Fracture behaviour in steel is a broad and complex topic that developed rapidly in the twentieth century driven by the demands of technological growth. The unexpected fracture of welded liberty ships at sea in World War 2; the need for reliable long term containment for the nuclear reactors in the 1950s and 1960s; and prevention of fatigue failures in aircraft frames since the 1950s all drove engineering research into steel fracture behaviour. There are many subtle variations in definitions in the published literature on fracture that can be confusing. Therefore an attempt has been made to clarify terminology. The term brittle fracture in particular is only used in this thesis as applying to running fracture when the general or far field tensile stresses are below the yield stress of the steel. The term pre-necking or running fracture is preferred to describe the condition more broadly which may occur prior to and also after general yielding, but before section necking. Running fracture is a manifestation of pre-necking fracture in which insufficient plastic flow is available in the assembly to absorb the energy released upon fracture. The experimental studies investigated the behaviour of constructional steel commonly used in New Zealand, at various levels of plastic strain. This started with Charpy V-Notch (CVN) testing which revealed that a significant transition temperature shift and curve shape change occurs with increasing plastic strain and the associated strain-hardening. This showed that the ability of steel to avoid pre-necking or running fracture reduces as the level of plastic strain-hardening increases. Temperature controlled Crack Tip Opening Displacement (CTOD) testing was then undertaken. The setting of testing temperatures for the CTOD tests were guided by review of the CVN test results, using published CVN to fracture toughness correlation methods. However running cleavage fractures developed in the CTOD specimens at higher than predicted temperatures of 10 oC and 20 oC. These are typical service temperatures for structures in New Zealand and so are very likely to occur at the time of an earthquake. The implication from this is that there are levels of strain-hardening and conditions of material notching constraint that can lead to pre-necking and running fracture in New Zealand fabricated steel structures, under severe earthquake loading. Care was taken in the CTOD testing to monitor and maximise the capture of data electronically using a specially developed Direct Current Potential Drop method. This allowed the test results to be analysed and considered in varying ways, leading to a consistent assessment of the CTOD, crack growth, and the specific work of fracture in each test piece. While CTOD test results have sometimes been published by structural and welding engineering researchers in the wake of Kobe and Northridge, the results were typically of little use for this study as the CTOD initiation point was generally not identified effectively. The effect of remote plastic flow in the specimens was also not adequately accounted for. The CTOD test results were often simply used to help correlate other factors observed by the researchers. Side-grooving of specimens was not reported as having been used in any of the published results reviewed. When conducting CTOD test with highly ductile constructional steels it is very difficult to get useful CTOD results if the specimens are not side-grooved, as significant necking and tunnelling will otherwise occur and limit the usefulness of the results. Work by Knott and also by McRobie and Smith was seminal in terms of identifying some critical aspects of plane strain development in CTOD tests, and the links to non-metallic particle density with respect to fracture toughness and CTOD at initiation. Some of their findings with regards to the effect of pre-strain on CTOD initiation were subsequently found to confirm the experimental findings in this study. No effective methodology for prediction of pre-necking or running fracture in a structural member or assembly when subjected to gross plastic cyclic deformation was found to exist in the literature. It was concluded however that the principles of specific work of fracture, and monotonic and cyclic fracture similitude were particularly relevant. These were therefore utilised in the development of the design method proposed in this thesis. The CTOD test results were reviewed, isolating the remote plastic flow component, to determine the critical specific work of fracture property Rc of the steels tested. A meeting with Professor Kuwamura at the University of Tokyo was providential, allowing discussion of his similitude principle, and observations in person of some of the fractured specimens developed during his full scale test series’. Running fractures with cleavage were evident in the specimens, with their tell-tale chevron markings. He had predicted running fracture problems in structures in Japan ahead of the Kobe earthquake and been largely ignored. His insights were subsequently seriously considered in Japan after the earthquake. He and his colleagues developed the principle of structural similitude that relates monotonic fracture displacement ductility to cyclic fracture displacement ductility for a particular assembly. This arose from their observation that running fractures developed from ductile crack formation at blunt notches in structures. The similitude principle has echoes of the Coffin-Manson approach to ductile crack initiated low cycle fracture. The principle of similitude has a log–log relationship as does the Manson-Coffin relationship. So where notch plasticity controls the initiation of fracture in a structural assembly it is conceptually reasonable to expect that the number of cycles to initiation of fracture from a notch will have a log–log relationship to the amplitude of the cyclic strain developed in the notch. Kuwamura found that steel assemblies with lower CVN energy had reduced cyclic fracture endurance than the same assemblies made with steel with higher CVN impact energy. However no method of predicting performance of any particular assembly could be developed from his observations. The benefit of his method primarily relates to the minimising of testing necessary to assess the fracture limited cyclic displacement ductility of a structural assembly. However it doesn’t provide a means for designing a structural assembly to achieve specific levels of ductile endurance other than clearly identifying the need to use steel with good CVN characteristics. The most significant development arising from this thesis is therefore the development of a design method to assess cyclic ductile endurance. The method utilises the specific work of fracture properties obtained from CTOD specimens of the steel in conjunction with a relatively simple fracture mechanics assessment and an elasto-plastic finite element analysis (FEA). The FEA model is used to determine the displacement ductility of the assembly at the calculated onset of pre-necking fracture. The elasto-plastic stress–strain properties of the steel in various pre-strain states required for the FEA may be derived from tensile testing. Kuwamura’s similitude principle is then used to predict cyclic plastic endurance at various constant displacement ductility amplitudes. The method is extended using Miner’s rule to allow for the effects of increasing variable amplitude cyclic plastic loading. In summary the thesis explains why pre-necking and running fractures occur in steel members subjected to cyclic plastic deformation during a severe earthquake. In addition a method for consistently assessing the ability of structural steel assemblies to achieve a specified level of ductile endurance during earthquakes is proposed. The method is verified against published results for a cyclic test of a simple steel member with a crack at mid-span. / Whole document restricted, but available by request, use the feedback form to request access.

This thesis provides a structural and materials engineering explanation for many of the running fractures that occurred in steel structures during the destructive Kobe and Northridge earthquakes in the mid 1990s. A method is developed that allows the ductile endurance of structural steel members subjected to cyclic plastic deformation during earthquakes to be assessed and for pre-necking running fractures to be avoided. The study commenced following the 2000 World Earthquake Conference in Auckland. The conference brought together the findings of the huge research effort, in America, Japan, Europe and New Zealand, that followed the Kobe and Northridge earthquakes. The running fractures that had occurred in steel structures represented an unpredicted failure mode that structural engineers have not known how to predict or suppress through the engineering design process. A clear fundamental understanding of the causes and how to prevent the fractures did not arise from the conference. In fact apparently conflicting results were reported. Full scale cyclic tests in New Zealand on structural assemblies had not resulted in running fractures, whereas tests in American and Japan had. Structural engineers designing earthquake resistant structures rely on constructional steel to be materially homogeneous and nominally tri-linear in behaviour. Steel is expected to behave elastically under regular in-service loading, have a reliable and flat yield stress-strain characteristic, and under overload then develop predictable levels of strain-hardening in conjunction with significant plastic elongation up to its ultimate tensile strength. Steel is expected to eventually fracture after further plastic elongation and necking. Ductile design strategies and methods utilise the plastic elongation characteristics of steel to protect structures in earthquake. Plastic deformation is considered to beneficially dissipate energy generated in the structure by a severe earthquake and also dampen the structure’s response. The occurrence of running fracture without significant cyclic plastic deformation and before section necking in steelwork, therefore undermines the basis of the ductile seismic design approach. The initial part of the thesis is devoted to bringing together the fundamental aspects of materials engineering related to fracture of constructional steel. This is intended to provide a bridge of knowledge for structural engineering practitioners and researchers not fully conversant with materials engineering aspects of fracture. Fracture behaviour in steel is a broad and complex topic that developed rapidly in the twentieth century driven by the demands of technological growth. The unexpected fracture of welded liberty ships at sea in World War 2; the need for reliable long term containment for the nuclear reactors in the 1950s and 1960s; and prevention of fatigue failures in aircraft frames since the 1950s all drove engineering research into steel fracture behaviour. There are many subtle variations in definitions in the published literature on fracture that can be confusing. Therefore an attempt has been made to clarify terminology. The term brittle fracture in particular is only used in this thesis as applying to running fracture when the general or far field tensile stresses are below the yield stress of the steel. The term pre-necking or running fracture is preferred to describe the condition more broadly which may occur prior to and also after general yielding, but before section necking. Running fracture is a manifestation of pre-necking fracture in which insufficient plastic flow is available in the assembly to absorb the energy released upon fracture. The experimental studies investigated the behaviour of constructional steel commonly used in New Zealand, at various levels of plastic strain. This started with Charpy V-Notch (CVN) testing which revealed that a significant transition temperature shift and curve shape change occurs with increasing plastic strain and the associated strain-hardening. This showed that the ability of steel to avoid pre-necking or running fracture reduces as the level of plastic strain-hardening increases. Temperature controlled Crack Tip Opening Displacement (CTOD) testing was then undertaken. The setting of testing temperatures for the CTOD tests were guided by review of the CVN test results, using published CVN to fracture toughness correlation methods. However running cleavage fractures developed in the CTOD specimens at higher than predicted temperatures of 10 oC and 20 oC. These are typical service temperatures for structures in New Zealand and so are very likely to occur at the time of an earthquake. The implication from this is that there are levels of strain-hardening and conditions of material notching constraint that can lead to pre-necking and running fracture in New Zealand fabricated steel structures, under severe earthquake loading. Care was taken in the CTOD testing to monitor and maximise the capture of data electronically using a specially developed Direct Current Potential Drop method. This allowed the test results to be analysed and considered in varying ways, leading to a consistent assessment of the CTOD, crack growth, and the specific work of fracture in each test piece. While CTOD test results have sometimes been published by structural and welding engineering researchers in the wake of Kobe and Northridge, the results were typically of little use for this study as the CTOD initiation point was generally not identified effectively. The effect of remote plastic flow in the specimens was also not adequately accounted for. The CTOD test results were often simply used to help correlate other factors observed by the researchers. Side-grooving of specimens was not reported as having been used in any of the published results reviewed. When conducting CTOD test with highly ductile constructional steels it is very difficult to get useful CTOD results if the specimens are not side-grooved, as significant necking and tunnelling will otherwise occur and limit the usefulness of the results. Work by Knott and also by McRobie and Smith was seminal in terms of identifying some critical aspects of plane strain development in CTOD tests, and the links to non-metallic particle density with respect to fracture toughness and CTOD at initiation. Some of their findings with regards to the effect of pre-strain on CTOD initiation were subsequently found to confirm the experimental findings in this study. No effective methodology for prediction of pre-necking or running fracture in a structural member or assembly when subjected to gross plastic cyclic deformation was found to exist in the literature. It was concluded however that the principles of specific work of fracture, and monotonic and cyclic fracture similitude were particularly relevant. These were therefore utilised in the development of the design method proposed in this thesis. The CTOD test results were reviewed, isolating the remote plastic flow component, to determine the critical specific work of fracture property Rc of the steels tested. A meeting with Professor Kuwamura at the University of Tokyo was providential, allowing discussion of his similitude principle, and observations in person of some of the fractured specimens developed during his full scale test series’. Running fractures with cleavage were evident in the specimens, with their tell-tale chevron markings. He had predicted running fracture problems in structures in Japan ahead of the Kobe earthquake and been largely ignored. His insights were subsequently seriously considered in Japan after the earthquake. He and his colleagues developed the principle of structural similitude that relates monotonic fracture displacement ductility to cyclic fracture displacement ductility for a particular assembly. This arose from their observation that running fractures developed from ductile crack formation at blunt notches in structures. The similitude principle has echoes of the Coffin-Manson approach to ductile crack initiated low cycle fracture. The principle of similitude has a log–log relationship as does the Manson-Coffin relationship. So where notch plasticity controls the initiation of fracture in a structural assembly it is conceptually reasonable to expect that the number of cycles to initiation of fracture from a notch will have a log–log relationship to the amplitude of the cyclic strain developed in the notch. Kuwamura found that steel assemblies with lower CVN energy had reduced cyclic fracture endurance than the same assemblies made with steel with higher CVN impact energy. However no method of predicting performance of any particular assembly could be developed from his observations. The benefit of his method primarily relates to the minimising of testing necessary to assess the fracture limited cyclic displacement ductility of a structural assembly. However it doesn’t provide a means for designing a structural assembly to achieve specific levels of ductile endurance other than clearly identifying the need to use steel with good CVN characteristics. The most significant development arising from this thesis is therefore the development of a design method to assess cyclic ductile endurance. The method utilises the specific work of fracture properties obtained from CTOD specimens of the steel in conjunction with a relatively simple fracture mechanics assessment and an elasto-plastic finite element analysis (FEA). The FEA model is used to determine the displacement ductility of the assembly at the calculated onset of pre-necking fracture. The elasto-plastic stress–strain properties of the steel in various pre-strain states required for the FEA may be derived from tensile testing. Kuwamura’s similitude principle is then used to predict cyclic plastic endurance at various constant displacement ductility amplitudes. The method is extended using Miner’s rule to allow for the effects of increasing variable amplitude cyclic plastic loading. In summary the thesis explains why pre-necking and running fractures occur in steel members subjected to cyclic plastic deformation during a severe earthquake. In addition a method for consistently assessing the ability of structural steel assemblies to achieve a specified level of ductile endurance during earthquakes is proposed. The method is verified against published results for a cyclic test of a simple steel member with a crack at mid-span. / Whole document restricted, but available by request, use the feedback form to request access.

This thesis provides a structural and materials engineering explanation for many of the running fractures that occurred in steel structures during the destructive Kobe and Northridge earthquakes in the mid 1990s. A method is developed that allows the ductile endurance of structural steel members subjected to cyclic plastic deformation during earthquakes to be assessed and for pre-necking running fractures to be avoided. The study commenced following the 2000 World Earthquake Conference in Auckland. The conference brought together the findings of the huge research effort, in America, Japan, Europe and New Zealand, that followed the Kobe and Northridge earthquakes. The running fractures that had occurred in steel structures represented an unpredicted failure mode that structural engineers have not known how to predict or suppress through the engineering design process. A clear fundamental understanding of the causes and how to prevent the fractures did not arise from the conference. In fact apparently conflicting results were reported. Full scale cyclic tests in New Zealand on structural assemblies had not resulted in running fractures, whereas tests in American and Japan had. Structural engineers designing earthquake resistant structures rely on constructional steel to be materially homogeneous and nominally tri-linear in behaviour. Steel is expected to behave elastically under regular in-service loading, have a reliable and flat yield stress-strain characteristic, and under overload then develop predictable levels of strain-hardening in conjunction with significant plastic elongation up to its ultimate tensile strength. Steel is expected to eventually fracture after further plastic elongation and necking. Ductile design strategies and methods utilise the plastic elongation characteristics of steel to protect structures in earthquake. Plastic deformation is considered to beneficially dissipate energy generated in the structure by a severe earthquake and also dampen the structure’s response. The occurrence of running fracture without significant cyclic plastic deformation and before section necking in steelwork, therefore undermines the basis of the ductile seismic design approach. The initial part of the thesis is devoted to bringing together the fundamental aspects of materials engineering related to fracture of constructional steel. This is intended to provide a bridge of knowledge for structural engineering practitioners and researchers not fully conversant with materials engineering aspects of fracture. Fracture behaviour in steel is a broad and complex topic that developed rapidly in the twentieth century driven by the demands of technological growth. The unexpected fracture of welded liberty ships at sea in World War 2; the need for reliable long term containment for the nuclear reactors in the 1950s and 1960s; and prevention of fatigue failures in aircraft frames since the 1950s all drove engineering research into steel fracture behaviour. There are many subtle variations in definitions in the published literature on fracture that can be confusing. Therefore an attempt has been made to clarify terminology. The term brittle fracture in particular is only used in this thesis as applying to running fracture when the general or far field tensile stresses are below the yield stress of the steel. The term pre-necking or running fracture is preferred to describe the condition more broadly which may occur prior to and also after general yielding, but before section necking. Running fracture is a manifestation of pre-necking fracture in which insufficient plastic flow is available in the assembly to absorb the energy released upon fracture. The experimental studies investigated the behaviour of constructional steel commonly used in New Zealand, at various levels of plastic strain. This started with Charpy V-Notch (CVN) testing which revealed that a significant transition temperature shift and curve shape change occurs with increasing plastic strain and the associated strain-hardening. This showed that the ability of steel to avoid pre-necking or running fracture reduces as the level of plastic strain-hardening increases. Temperature controlled Crack Tip Opening Displacement (CTOD) testing was then undertaken. The setting of testing temperatures for the CTOD tests were guided by review of the CVN test results, using published CVN to fracture toughness correlation methods. However running cleavage fractures developed in the CTOD specimens at higher than predicted temperatures of 10 oC and 20 oC. These are typical service temperatures for structures in New Zealand and so are very likely to occur at the time of an earthquake. The implication from this is that there are levels of strain-hardening and conditions of material notching constraint that can lead to pre-necking and running fracture in New Zealand fabricated steel structures, under severe earthquake loading. Care was taken in the CTOD testing to monitor and maximise the capture of data electronically using a specially developed Direct Current Potential Drop method. This allowed the test results to be analysed and considered in varying ways, leading to a consistent assessment of the CTOD, crack growth, and the specific work of fracture in each test piece. While CTOD test results have sometimes been published by structural and welding engineering researchers in the wake of Kobe and Northridge, the results were typically of little use for this study as the CTOD initiation point was generally not identified effectively. The effect of remote plastic flow in the specimens was also not adequately accounted for. The CTOD test results were often simply used to help correlate other factors observed by the researchers. Side-grooving of specimens was not reported as having been used in any of the published results reviewed. When conducting CTOD test with highly ductile constructional steels it is very difficult to get useful CTOD results if the specimens are not side-grooved, as significant necking and tunnelling will otherwise occur and limit the usefulness of the results. Work by Knott and also by McRobie and Smith was seminal in terms of identifying some critical aspects of plane strain development in CTOD tests, and the links to non-metallic particle density with respect to fracture toughness and CTOD at initiation. Some of their findings with regards to the effect of pre-strain on CTOD initiation were subsequently found to confirm the experimental findings in this study. No effective methodology for prediction of pre-necking or running fracture in a structural member or assembly when subjected to gross plastic cyclic deformation was found to exist in the literature. It was concluded however that the principles of specific work of fracture, and monotonic and cyclic fracture similitude were particularly relevant. These were therefore utilised in the development of the design method proposed in this thesis. The CTOD test results were reviewed, isolating the remote plastic flow component, to determine the critical specific work of fracture property Rc of the steels tested. A meeting with Professor Kuwamura at the University of Tokyo was providential, allowing discussion of his similitude principle, and observations in person of some of the fractured specimens developed during his full scale test series’. Running fractures with cleavage were evident in the specimens, with their tell-tale chevron markings. He had predicted running fracture problems in structures in Japan ahead of the Kobe earthquake and been largely ignored. His insights were subsequently seriously considered in Japan after the earthquake. He and his colleagues developed the principle of structural similitude that relates monotonic fracture displacement ductility to cyclic fracture displacement ductility for a particular assembly. This arose from their observation that running fractures developed from ductile crack formation at blunt notches in structures. The similitude principle has echoes of the Coffin-Manson approach to ductile crack initiated low cycle fracture. The principle of similitude has a log–log relationship as does the Manson-Coffin relationship. So where notch plasticity controls the initiation of fracture in a structural assembly it is conceptually reasonable to expect that the number of cycles to initiation of fracture from a notch will have a log–log relationship to the amplitude of the cyclic strain developed in the notch. Kuwamura found that steel assemblies with lower CVN energy had reduced cyclic fracture endurance than the same assemblies made with steel with higher CVN impact energy. However no method of predicting performance of any particular assembly could be developed from his observations. The benefit of his method primarily relates to the minimising of testing necessary to assess the fracture limited cyclic displacement ductility of a structural assembly. However it doesn’t provide a means for designing a structural assembly to achieve specific levels of ductile endurance other than clearly identifying the need to use steel with good CVN characteristics. The most significant development arising from this thesis is therefore the development of a design method to assess cyclic ductile endurance. The method utilises the specific work of fracture properties obtained from CTOD specimens of the steel in conjunction with a relatively simple fracture mechanics assessment and an elasto-plastic finite element analysis (FEA). The FEA model is used to determine the displacement ductility of the assembly at the calculated onset of pre-necking fracture. The elasto-plastic stress–strain properties of the steel in various pre-strain states required for the FEA may be derived from tensile testing. Kuwamura’s similitude principle is then used to predict cyclic plastic endurance at various constant displacement ductility amplitudes. The method is extended using Miner’s rule to allow for the effects of increasing variable amplitude cyclic plastic loading. In summary the thesis explains why pre-necking and running fractures occur in steel members subjected to cyclic plastic deformation during a severe earthquake. In addition a method for consistently assessing the ability of structural steel assemblies to achieve a specified level of ductile endurance during earthquakes is proposed. The method is verified against published results for a cyclic test of a simple steel member with a crack at mid-span. / Whole document restricted, but available by request, use the feedback form to request access.

京都大学 / 新制・課程博士 / 博士(生命科学) / 甲第23337号 / 生博第455号 / 新制||生||61(附属図書館) / 京都大学大学院生命科学研究科高次生命科学専攻 / (主査)教授 松崎 文雄, 教授 影山 龍一郎, 教授 見学 美根子 / 学位規則第4条第1項該当 / Doctor of Philosophy in Life Sciences / Kyoto University / DFAM