In agreement with previous work, adult rats given selective lesions to dopamine (DA)-containing neurons as neonates exhibited a greater behavioral sensitization to repeated phencyclidine (PCP) treatment in comparison to sham-lesioned controls. Acute administration of olanzapine (1-5 mg/kg ip) or clozapine (15 mg/kg ip) decreased sensitized PCP-induced activity in both lesioned and control animals. Acute haloperidol (0.5 mg/kg ip) had no impact on PCP responsiveness in lesioned animals, but significantly antagonized PCP effects in sham-lesioned controls. Ketanserin, a selective 5-HT 2A/5-HT2C-receptor antagonist, significantly reduced PCP activation in both lesioned and control rats, suggesting that the efficacy of atypical antipsychotics against PCP-induced sensitized responses may be mediated by one of the 5-HT2-receptor subtypes. A 6-week chronic regimen of orally administered olanzapine, clozapine, or haloperidol failed to block the sensitization induced by repeated PCP exposure. However, a 10-month oral olanzapine treatment significantly blunted the behavioral sensitization to repeated PCP exposure in lesioned animals, even after withdrawal from chronic olanzapine for more than 3 weeks. A 10-month oral haloperidol treatment had no effect on the sensitization induced by repeated PCP dosing. The persistent effect of chronic olanzapine administration on PCP sensitization may be relevant to the chronic therapeutic efficacy of atypical antipsychotics treating schizophrenia - a clinical syndrome linked to enhanced sensitivity to N-methyl-D-aspartate (NMDA)-receptor antagonists.

In order to dissect social behaviors into their smallest components, five interactions, each involving one neonate and one adult, were video taped and kinescoped into 16mm black and white film exposed at 24 frames per second. The film was analyzed frame by frame and all starts, stops, and changes in direction of movements were scored. Adult movement durations were significantly shorter during vocalization and longer during nonvocalization. Each infant and adult body part had its own characteristic movement rate. Almost two-thirds of the dyadic movements were interactionally synchronous and most of the synchronization occurred during vocalization. It is suggested that this behavior comprises an interactional code, innately biased to encourage mutual attachment between parents and infants. Further, it is suggested that the neonate is continually decoding incoming family socialization messages and encoding and relaying her/his own temperament messages which may or may not be harmonious with the overall family temperament.

No description available.

Objectives: To assess the use of the “Core Competencies for Public Health Professionals” standards in practice and academic work settings by public health nurses (PHNs), and to determine differences between practitioners and faculty. Design: Nonexperimental, descriptive study using a cross‐sectional survey. Sample: Three public health nursing (PHN) organizations sent invitations to all members. A total of 334 (18.7%) from an estimated 1,786 members completed the survey. Measurements: The investigators developed a 17‐item web‐based survey with open‐ and closed‐ended responses, using Rogers' diffusion of innovations as a theoretical framework. Results: Respondents are equally familiar with the competencies for public health professionals disseminated by the Council on Linkages and for PHNs by the Quad Council of Public Health Nursing Organizations (Quad Council). Two thirds of PHNs are aware of the competencies after only 2 years, primarily from professional PHN organizations. Faculty are adopting and using the competencies at a significantly faster rate than practitioners. Conclusions: Faculty and practitioners who use the competencies value them, and rarely discontinued their use after adoption. Efforts to promote diffusion among faculty and especially practitioners need to continue. Professional organizations can actively provide and share examples of useable formats and best practices associated with the competencies.

Human milk contains ~ 25 µg/mL of soluble cluster of differentiation 14 (sCD14) protein, a pattern recognition receptor (PRR) that triggers the innate immune system to respond to bacterial lipopolysaccharide (LPS). To date, the role of CD14 in the digestive tract of breast fed infants has not been well characterized and is the subject of this thesis. To investigate the biodistribution of proteins such as CD14 in vivo, a novel method for 14C radiolabeling of proteins to high specific radioactivity was developed using in vacuo methylation. Bovine serum albumin (BSA) and casein were used as test proteins to determine the following: 1) The efficacy of the in vacuo radiolabeling procedure; 2) The extent of incorporation of the 14C-label into the organs of oro-gastric gavaged 10 day old Sprague Dawley rats. [14C]BSA, [14C]casein and [14C]CD14 were prepared with specific radioactivities of 10 400, 10 800 and 163 000 dpm/µg, respectively. After feeding 6.25 µg of 14C-labeled proteins, quantifiable levels of 14C were found in the stomach, jejunum, duodenum, ileum, large intestine, intestinal luminal flushes, blood, liver, spleen and kidneys of rats. The accumulation of radiolabel in the organs of [14C]CD14 fed rats was temporally and spatially distinct from [14C]BSA and [14C]casein. Most notably, the label persisted in the stomach 480 min post-gavage. To design a neonate animal model for biodistribution, the segmental and total gastrointestinal transit times (GItt) were measured in two litters of 10 and 15 day old Sprague Dawley rat pups using barium sulfate. Ten day old rat pups that remained with and without the dam had a total gastrointestinal transit time of 13.8 ± 0.9 hr and 9.3 ± 0.7 hr, respectively. This decrease (p<0.05) in total gastrointestinal transit time in the absence of the dam was age dependent, as it was not observed (p>0.05) in the 15 day old rat pup litter. The immunological impact of an exogenous sCD14 source was examined in human peripheral blood mononuclear cells (PBMC). Pre-treatment of CD14+ monocytes with sCD14 had a protective effect, one of reducing the production of proinflammatory cytokines (TNF-α, IL-6, IL-8, IL-1β) when challenged with LPS. 14C was absorbed by neonate rats upon ingestion of [14C]CD14 and exposure to relatively high concentrations of rCD14 led to a reduction in inflammation. This may be beneficial to initial gut colonization in breast-fed newborns. / Alexander Graham Bell NSERC CGS M scholarship. Japan Society for the Promotion of Sciences, Summer in Japan Fellowship. Funded by the Canadian Institutes of Health Research, Institute of Nutrition Metabolism and Diabetes Grant #82816 “Fate and function of breast milk and recombinant human CD14 at mammary and newborn gastrointestinal mucosal epithelia”.

Exposure to excessive noise during a neonates stay in the neonatal intensive care unit (NICU) can create both immediate and long term health problems such as, hearing loss, neurological deficits, and sleep pattern disturbances. The use of earmuffs or earplugs to decrease the neonate’s exposure to noise can create a more stable environment to facilitate improved growth and development. The purpose of this research was to examine the use of earmuffs or earplugs to reduce the impact of noise on neonates. A systematic review of literature was conducted using online databases including CINAHL, ERIC, Ebsco Host, Medline, and PsychINFO. The search included a combination of the following terms: ‘preterm’, ‘neonate’, ‘NICU’, ‘noise’, ‘earplugs’, and ‘earmuffs’. Peer reviewed, articles published in the English language were examined that tested noise reduction devices in the NICU setting, hearing screening of neonates, and the effect of noise reduction interventions on physiologic changes in the preterm infant receiving care in the NICU. Results yielded 8 articles between the years of 1995 to 2017 which were synthesized for review. The results indicated that the use of earmuffs or earplugs in the NICU may offer a viable solution to reducing the effects of excessive noise on neonate’s growth and development. The use of earmuffs or earplugs has been shown to positively improve vital signs, increase growth, improve physiological and motor development, and improve sleep efficiency. Further research on larger sample sizes is needed in order to validate the findings and offer substantial evidence for its use in the clinical setting.

A fetus hears and responds to maternal sounds as early as 26 weeks' gestation. When born prematurely, a neonate may face health challenges due to overall organ immaturity and hospitalization in the Neonatal Intensive Care Unit, where developmentally important maternal sounds are replaced with routine hospital noise. A potential intervention that can provide meaningful auditory stimulation these neonates lack is implementation of maternal sound interventions. These interventions replicate the intrauterine auditory environment by playing recorded maternal speech and heart sounds in the incubator. A literature review was completed to identify effects on neurodevelopmental, nutritional, and physiological measurements this intervention may have on premature neonates. A review of the literature was conducted using the databases CINAHL Plus with Full Text, Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews, MEDLINE, and PsycINFO. Search terms utilized were: Voice/Sound; preterm/prematur*; neonat*/infant*/newborn; and matern*/mother*. Results were refined using limiters of peer-reviewed, publication date of 2012-2017, and English language. Twelve articles met the criteria for review. The maternal sounds intervention was found to correlate with improved neurodevelopment in the first months of life, especially relating to auditory and language areas of the brain. Nutritional outcomes were positive, but studies were inconsistent with findings. The physiological measurements were positively affected, with strong evidence of a calming effect, and lowering of the heart rate. Results indicated recorded maternal sound interventions were associated with positive health outcomes in premature neonates. Further research with larger sample sizes and uniform study designs are needed to validate the findings.

The prevalence of herpes simplex virus (HSV) infection globally is high, and although there is no cure for it, the antiviral drug acyclovir is used to alleviate symptoms. There are two types of HSV: HSV-1, which typically infects the oral area, and HSV-2, which is associated with genital infections. A mother who carries the infection may transmit it to a neonate in different ways, most commonly via vaginal delivery in the presence of active lesions. There are three types of HSV disease that affect newborns: skin, eyes or mouth (SEM) disease, central nervous system (CNS) disease, or disseminated disease. The purpose of this study was to examine the long-term effects of the infection and the treatment used in neonates infected with HSV. Data collection consisted of original case reports published in Medline, CINHAL, and Google Scholar. Two case reports were found, and this narrative review compares the cases, which report recurrences and outcomes of HSV infection identified in the three databases. Both cases were consistent with recurrence of CNS disease, and one showed signs of a slight developmental delay that may have been related to the CNS insult.

Systematic Review / Advancements in medicine have resulted in decreased neonatal mortality and morbidity associated with congenital anomalies (CA). Unfortunately, the advantages of these developments have been confined to high-income countries (HICs), demonstrated by the comparatively high incidence of congenital anomalies in low and low-middle-income countries (LLMICs). Evidence suggests that neonates in LLMICs encounter considerably more barriers to care than those in HICs due to a malfunctioning referral system and poorly implemented health policies that hinder the timely provision of care. As many CA are now accepted as surgically treatable, the purpose of this study was to understand what inhibits the success of a neonate from obtaining surgery in LLMICs and how that could be improved. Seven databases were searched in this systematic review to identify articles on neonates with surgically treatable CA. A total of 370 studies were identified for screening; 16 were included in the final analysis. Studies were screened and selected individually by two researchers based on the research question, and all disagreements were resolved jointly. Studies were reviewed for factors affecting the delivery of surgical treatment and were then coded as a barrier or a facilitator. Barriers to care were identified in every study, and suggested facilitators were offered by the authors, but these facilitators were not tested in the studies. This study contributes to the literature by providing additional detail on what is known about the surgical referral system in LLMICs. The study findings will inform policymakers and local governments of the realities faced by neonates and their caregivers while navigating through the surgical referral system and establish the need for alternate policy implementation strategies. / Thesis / Master of Science (MSc) / Congenital anomalies (CA) have been identified as a significant contributor to the global burden of disease, accounting for 25.3-38.8 million disability-adjusted life-years worldwide. Many CA have been classified as surgically treatable however, approximately 295,000 neonates die annually due to these conditions. As 94% of CA occur in low- and low- middle-income countries (LLMICs), this study aims to elucidate any barriers and facilitators that may influence accessing surgical treatment. A systematic review has been selected to synthesize the literature regarding what is known about accessing surgery for neonates with CA in LLMICs.

No description available.