The thesis deals with the issue of drinking water. The aim of the study is to evaluate quality parameters of drinking water in connection with legislation attending to this issue. The evaluation was conducted in nine agricultural subjects and analyzes of drinking water were determined and evaluated. Microbiological and hydrochemical indicators of drinking water were compared within the year 2015. Regarding microbiological indicators, coliforms, Escherichia coli, enterococci, psychrophilic bacteria were followed. Ammonia nitrogen, nitrate nitrogen, phosphate phosphor, total inorganic carbon, total organic carbon, total carbon, total nitrogen, pH, conductivity were followed regarding hydrochemical indicators. The final values showed exceeded values of the microbiological indicators at five sampling sites according to applicable legislation. Furthermore, threshold values of total organic carbon were exceeded in all tested agricultural subjects and also values of nitrate nitrogen in drinking water were increased at three subjects evaluated throughout the year.

This thesis deals with the evaluation of concentration levels of phosphates, nitrates and other parameters (pH, conductivity, suspended solids, alkalinity, total carbon, inorganic carbon, total organic carbon and total nitrogen) in fishpond systems at Tabor. Further was to consider the difference in value depending on the age of the systems and agricultural activities in their vicinity. Samples were taken in season 2015 Liderovické pond system (2 sampling points at the top of the system, one sampling point in the outflow from the system), Radimovická pond system (one sampling point at the top of the systém, one sampling point at the outlet of the system) and Jordan ( one sampling point at the outfall of the pond). The highest differences were found in the values of conductivity from 279 S.cm-1 to 1230 S.cm-1, the values of total carbon from 18.75 mg.l-1 to 67.33 mg.l-1, and the values of suspended solids from 1 mg.l-1 to 117.33 mg.l-1. The gradual increase in the value of NO3-N and PO4-P was registered during the season with Jordan. Based on the results we can say that the older (Radimovická system) showed higher levels of total carbon and younger (Liderovická system) showed higher NO3-N depending on land use in the surrounding area.

This diploma thesis deals with clarification of an influence of rain discharge events on nutrients loss from drainage area and which rain discharge events are the main cause of the loss. Within the scope of this the influence of these episodes on drainage area of the Jenínský brook will be quantified, more specifically sub-drainage area J2. The thesis describes not only the circle of water in the nature, specification of rain drainage events origin, but also the very factors which influence the water drainage and the loss of nutrients caused by the drainage. Furthermore, the basic nutrients which appear the most often and in the biggest quantities in our waters are described, these being nitrogen and phosphor and last but not least what is described here is the total loss of nutrients in water during rain drainage events. The description of a drainage area itself and a water intake profile J2 follows. The water intake profile J2 was the source for processing the results. The results were processed by several computational methods, which were compared to each other in the end. The loss during 5 extreme rain events was calculated and so was their percentage representation in the total annual losses and the consecutive evaluation of the results.

Introdução: O tratamento com nitrato fixo em pacientes com angina estável é comum na prática clínica. Contudo, os eventos adversos podem interferir na qualidade de vida relacionada à saúde (QVRS) e adesão ao tratamento medicamentoso. O objetivo do estudo foi comparar a QVRS e adesão ao tratamento em grupos de pacientes com angina estável em retirada e manutenção de nitrato. Métodos: Ensaio clínico randomizado conduzido com pacientes com angina estável em dois grupos: intervenção com retirada de nitrato e introdução de placebo e controle com manutenção de nitrato. Ambos os grupos receberam três avaliações: basal, 30 dias e 120 dias no período de quatro meses. A QVRS foi mensurada pelo Short Form Health Survey (SF-36) e Seattle Angina Questionnaire (SAQ), e a adesão foi aferida pela escala de Morisky e contagem de comprimidos. Resultados: 105 pacientes foram randomizados (51: placebo e 54: nitrato). Após quatro meses, observou-se maiores escores nos domínios de dor (63+19; 67+23; 72+24; P=0,004) e aspecto social (87+19; 91+21; 91+19; P=0,002) do SF-36 e no escore de limitação física (74+11; 78+10; 78+8; P=0,006) do SAQ no grupo nitrato, e redução do escore de estabilidade da angina (81+19; 72+20; 72+24; P=0,031) no grupo placebo. Contudo, o tamanho de efeito foi igual ou inferior a 0,42 nesses domínios. A adesão aferida no final entre os grupos foi significativamente maior no grupo intervenção (94% vs 80%; P=0,041) com a escala de Morisky, mas não houve diferença entre os grupos na contagem da medicação. Conclusão: Pacientes com angina estável em uso de nitrato em comparação aos pacientes com uso de placebo não tiveram comprometimento clinicamente relevante da QVRS, porém demonstraram pior adesão ao tratamento na escala de Morisky. / Background: Long-term nitrate treatment of stable angina is associated with adverse events and can interfere with health-related quality of life (HRQoL) and medication adherence. The aim of the present study was to compare HRQoL and adherence to treatment in patients with stable angina undergoing nitrate withdrawal and maintenance. Methods: Randomized clinical trial. Patients were allocated into an intervention group (nitrate withdrawal followed by introduction of placebo) or a control group (nitrate maintenance). The assessments were performed at baseline, 30 days, and 120 days with the Short Form Health Survey (SF-36) and the Seattle Angina Questionnaire (SAQ). Treatment adherence was measured by the Morisky scale and pill count. Results: 51 participants were randomized for replacement of nitrate with placebo and 54 for maintenance of treatment with nitrate. After four months, SF-36 scores increased for the bodily pain (P=0.004) and social functioning (P=0.002) in the nitrate maintenance group. Increased SAQ scores were also noted for physical limitations (P=0.006) in the nitrate maintenance group. SAQ score for angina stability (P=0.031) decreased in the placebo group. However, the effect size was small ! 0.42 in those domains. At the end of the study, adherence was significantly higher in the placebo group (P=0.041), but no difference was detected between the groups with the pill count method. Conclusion: HRQoL was similar in patients with stable angina using nitrate regularly as compared to patients undergoing nitrate withdrawal. However, adherence to treatment was lower in nitrate users according to the Morisky scale.

This work aimed to evaluate long-term changes of selected chemical and physical parameters of rainwater from their own rain gauge stations Stropnicko. Chemical and physical parameters influence the rate of loss of substance in the landscape through the soil environment. In particular, the amount and concentration of dissolved sulphates and nitrates, affecting the value of the current pH. Samples were taken from their own rain gauge stations Stropnicko. Names of stations from which samples were taken are Horní Stropnice, Nakolice, Paseky and Nové Hrady. Rainfall were measured between the years 2005-2011 and were subjected to chemical analysis.

Le monde fait face à une crise environnementale sans précédent, induite par l’action toujours plus marquée de l’homme sur son milieu. Depuis le début de l’ère industrielle, l’utilisation massive des énergies fossiles, a provoqué un dérèglement climatique planétaire. Les microalgues offrent la possibilité de produire des biocarburants avec une empreinte carbone réduite mais nécessitent encore de nombreuses améliorations pour être économiquement viables. Une de ces améliorations, à l’instar de l’agriculture moderne, réside dans la sélection de souches plus productives. Dans ce travail de thèse, nous avons développé la sélection par pression continue, consistant à utiliser les processus de l’évolution pour faire émerger des populations d’intérêt.Une première voie explorée a consisté à utiliser la température, paramètre crucial de la croissance des microalgues, comme moteur de sélection. En soumettant une culture à des variations diurnes de température durant une année, nous sommes parvenus à adapter une souche de Tisochrysis lutea à une gamme de températures plus large, la rendant donc plus tolérante aux variations de ce paramètre.La seconde voie a cherché à accroitre la capacité de Cylindrotheca closterium à emmagasiner ou au contraire à mobiliser son azote intracellulaire, propriété physiologiquement liée à sa capacité à produire des lipides. En forçant une population de microalgues à s'adapter à des apports discontinus d'azote (succession d'états de satiété et de carence), il a été possible de sélectionner les individus les plus riches en lipides.Enfin, nous avons modifié l'appareil pigmentaire de Tisochrisis lutea pour la rendre plus transparente à la lumière. En soumettant cette espèce à une succession de chocs lumineux, il a été possible de sélectionner les individus possédant les antennes photosynthétiques les plus petites, permettant une productivité accrue. / The world faces an unprecedented environmental crisis, led by the action always more marked with the man on its environment. From the beginning of the industrial era, the massive use of the fossil fuels, caused a global climatic disorder.

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