The present study analyzes cranial nonmetric traits used in forensic ancestry estimation on contemporary skeletal remains of modern European Americans in order to determine if there are statistically significant differences between males and females in trait expression. Research on cranial nonmetric traits for ancestry estimation has largely ignored the effects of sexual dimorphism on trait expression; however, there is growing evidence that some traits may be impacted by sex, among other variables. The 17 macromorphoscopic traits described in Hefner and Linde (2018) and the six mandibular morphoscopic traits described in Berg (2008) were scored on 97 females and 113 males from the Texas State University Donated Skeletal Collection in San Marcos, Texas. Chi-square tests were used to analyze if there are statistically significant cranial nonmetric trait expressions between males and females. From these tests, the results indicate that 14 out of the 23 cranial and mandibular nonmetric traits are statistically significantly different between the sexes, with a p-value less than 0.05. Gonial angle flare is the most significant feature, while the zygomaticomaxillary suture is the least significant feature. Additionally, correspondence analyses (CA) show the relationship between each cranial nonmetric trait score, that demonstrated significance, and both sexes. Ultimately, this research demonstrates that several nonmetric traits used in ancestry estimation are affected by sex; thus, it may be beneficial to develop sex-specific ancestry models for nonmetric traits.

Secular change has been documented in a number of studies focused on cranial and postcranial morphometrics and nonmetric traits. However, to date, few studies have addressed the potential of temporal change occurring in the expression of cranial nonmetric traits utilized in ancestry estimation. This study examines the effect of secular change on the expression of 23 cranial and mandibular nonmetric traits frequently employed in ancestry estimation; with age-at-death, sex, and year-of-birth of each individual documented for data analysis. Data were collected from European American individuals from the Hamann-Todd Skeletal Collection (n=518) and from the William M. Bass Donated Skeletal Collection (n=602). Individuals were divided into birth-year cohorts as follows: Hamann-Todd Skeletal Collection: 1824-1849 (Cohort 1), 1850-1874 (Cohort 2), 1875-1899 (Cohort 3), and 1900-1924 (Cohort 4); William M. Bass Donated Skeletal Collection: 1900-1924 (Cohort 4), 1925-1949 (Cohort 5), and 1950-1987 (Cohort 6). Pearson’s chi-square analyses produced significant p-values (≤ 0.01) in 19 of the 23 traits between the six birth-year cohorts. Factor maps generated through correspondence analyses were used as visual representations of relative trait expression between the cohorts. Ordinal regression analyses assessed the degree of variation between each cohort in relation to Cohort 1 along with the influence of age-at-death and sex on trait expression. Overall, analyses of the data revealed that secular change has occurred in 11 of the 23 traits, including: anterior nasal spine (ANS), malar tubercle (MT), nasal bone contour (NBC), postbregmatic depression (PBD), supranasal suture (SPS), transverse palatine suture (TPS), zygomaticomaxillary suture (ZS), gonial angle flare (GAF), mandibular tori (MDT), and posterior ramus edge inversion (PREI). Change in trait expression occurred in both males and females in seven traits, including: ANS, MT, TPS, ZS, GAF, MDT, and PREI. Significant change in trait expression occurred predominately between Cohorts 3 and 4 (birth years ranging from 1875 to 1924) and Cohorts 4 and 5 (birth years ranging from 1900 to 1949). This study demonstrates that secular change in nonmetric cranial and mandibular traits has occurred over the last two centuries with the greatest change appearing at the turn of the twentieth century.

This study seeks to distinguish the presence of secular change in contemporary nonmetric trait expression and explores the efficacy of morphological ancestry estimation with regards to North American indigenous groups. The study was conducted using cranial and mandibular trait data collected from two indigenous samples originating from the American Southwest: a pre-Contact sample from the American Museum of Natural History (n=150) and a modern sample from the New Mexico Decedent Image Database (n=100). To observe the modern sample, the RadiAnt DICOM viewer program was used to transform CT scan stacks into 3D models. Pearson’s chi-square analyses were used to assess the presence of statistically significant difference between nonmetric trait expression between the two samples. The analyses produced significant p-values (≤0.05) in 22 of the 24 observed traits. Using binary logistic regression equations, four models were developed to assess which traits contributed significantly to predicting group membership: Model 1 combined cranial and mandibular traits, Model 2 used seven of Hefner’s (2009) 11 macromorphoscopic traits, Model 3 used only cranial traits, and Model 4 used only mandibular traits. To evaluate the efficacy of an extant ancestry estimation method on indigenous sample, data from both the pre-Contact and modern samples were entered into the hefneR decision support system. The results indicate that the hefneR algorithm does not produce reliable ancestry estimates for either pre-Contact or modern indigenous samples; the samples received 14% and 11% correct classification, respectively. This study demonstrates that secular change has affected nonmetric trait expression in indigenous groups and that pre-Contact samples should not be used as proxies for modern populations.

This study surveyed floristic composition and distribution of weed vegetation in Mituo County. 206 relevés were surveyed according to relevé method. A total of 140 species belonging to 32 families of the vascular plants were recorded. The weed communities were classified with nonmetric multidimentional scaling, two-way indicator species analysis, tabular comparison method, fidelity and synoptic table analysis. Discriminate analysis was used to evaluate the distinctness of classification unitsal vegetation classification system was made using Braun-Blanquet approach of floristic-sociological classification in lower levels and physiognomic-sociological classification in higher levels. In floristic-sociological classification, assication is the basic unit, and it should be grouped into higher units (alliance) by floristic composition. The results showed 1 formation class, 2 formations in phsiogonomic units, and 4 alliances, 6 associations in floristic units: I. Lower montane-lowland weed vegetation formation A. Echinochloa colona alliance a. Echinochloa colona association b. Trianthemum portulacastrum association c. Panicum maximum association B. Dichanthium aristatum alliance d. Dichanthium aristatum association C. Eriochloa procera alliance e. Eriochloa procera association II. Sand dune vegetation formation D. Ipomoea pescaprae subsp. brasiliensis alliance f. Ipomoea pescaprae subsp. brasiliensis association

During oil-sands mining all vegetation cover, soil, overburden and oil-sand is removed, leaving pits several kilometres wide and hundreds of metres deep. These pits are reclaimed by a variety of treatments using mineral soil or a mixed peat and mineral soil as the capping layer and planted with trees with natural colonisation from adjacent sites. A number of reclamation treatments covering different age classes were compared with a range of natural forest ecotypes to identify the age at which the treatments become similar to a natural site with respect to vegetation composition and key soil attributes relevant to nutrient cycling. Ecosystem function was estimated from plant community composition, litter decomposition, development of an organic layer and bio-available nutrients. Key response variables including moisture, pH, C:N ratios, bio-available nutrients and ground-cover were analysed by non-metric multidimensional scaling and cluster analysis to discover which reclamation treatments were moving towards or merging with natural forest ecotypes and at what age this occurs. On reclaimed sites, bio-available nutrients including nitrate generally were above the natural range of variability but ammonium, phosphorus, potassium, sodium and manganese were generally very low and limiting to ecosystem development. Plant diversity was similar to natural sites from 5 years to 30 years after reclamation, but declined as reclaimed sites approached canopy closure. Grass and forb leaf litters decomposed faster than aspen or pine in the first year, but decomposition on one reclamation treatment fell below the natural range of variability. Development of an organic layer appeared to be facilitated by the presence of shrubs, while forbs correlated negatively with first-year decomposition of aspen litter. The better restoration amendments for tailings sands involved repeated fertilisation of peat: mineral mixtures in the early years of plant establishment, these became similar to a target ecotype at about 25 years. Good results were also shown by subsoil laid over non-saline overburden and fertilised once, these became similar to a target ecotype at about 15 years. Other treatments receiving a single application of fertiliser remain entrenched in the early reclamation phase for up to 25 years.

During oil-sands mining all vegetation cover, soil, overburden and oil-sand is removed, leaving pits several kilometres wide and hundreds of metres deep. These pits are reclaimed by a variety of treatments using mineral soil or a mixed peat and mineral soil as the capping layer and planted with trees with natural colonisation from adjacent sites. A number of reclamation treatments covering different age classes were compared with a range of natural forest ecotypes to identify the age at which the treatments become similar to a natural site with respect to vegetation composition and key soil attributes relevant to nutrient cycling. Ecosystem function was estimated from plant community composition, litter decomposition, development of an organic layer and bio-available nutrients. Key response variables including moisture, pH, C:N ratios, bio-available nutrients and ground-cover were analysed by non-metric multidimensional scaling and cluster analysis to discover which reclamation treatments were moving towards or merging with natural forest ecotypes and at what age this occurs. On reclaimed sites, bio-available nutrients including nitrate generally were above the natural range of variability but ammonium, phosphorus, potassium, sodium and manganese were generally very low and limiting to ecosystem development. Plant diversity was similar to natural sites from 5 years to 30 years after reclamation, but declined as reclaimed sites approached canopy closure. Grass and forb leaf litters decomposed faster than aspen or pine in the first year, but decomposition on one reclamation treatment fell below the natural range of variability. Development of an organic layer appeared to be facilitated by the presence of shrubs, while forbs correlated negatively with first-year decomposition of aspen litter. The better restoration amendments for tailings sands involved repeated fertilisation of peat: mineral mixtures in the early years of plant establishment, these became similar to a target ecotype at about 25 years. Good results were also shown by subsoil laid over non-saline overburden and fertilised once, these became similar to a target ecotype at about 15 years. Other treatments receiving a single application of fertiliser remain entrenched in the early reclamation phase for up to 25 years.

During oil-sands mining all vegetation cover, soil, overburden and oil-sand is removed, leaving pits several kilometres wide and hundreds of metres deep. These pits are reclaimed by a variety of treatments using mineral soil or a mixed peat and mineral soil as the capping layer and planted with trees with natural colonisation from adjacent sites. A number of reclamation treatments covering different age classes were compared with a range of natural forest ecotypes to identify the age at which the treatments become similar to a natural site with respect to vegetation composition and key soil attributes relevant to nutrient cycling. Ecosystem function was estimated from plant community composition, litter decomposition, development of an organic layer and bio-available nutrients. Key response variables including moisture, pH, C:N ratios, bio-available nutrients and ground-cover were analysed by non-metric multidimensional scaling and cluster analysis to discover which reclamation treatments were moving towards or merging with natural forest ecotypes and at what age this occurs. On reclaimed sites, bio-available nutrients including nitrate generally were above the natural range of variability but ammonium, phosphorus, potassium, sodium and manganese were generally very low and limiting to ecosystem development. Plant diversity was similar to natural sites from 5 years to 30 years after reclamation, but declined as reclaimed sites approached canopy closure. Grass and forb leaf litters decomposed faster than aspen or pine in the first year, but decomposition on one reclamation treatment fell below the natural range of variability. Development of an organic layer appeared to be facilitated by the presence of shrubs, while forbs correlated negatively with first-year decomposition of aspen litter. The better restoration amendments for tailings sands involved repeated fertilisation of peat: mineral mixtures in the early years of plant establishment, these became similar to a target ecotype at about 25 years. Good results were also shown by subsoil laid over non-saline overburden and fertilised once, these became similar to a target ecotype at about 15 years. Other treatments receiving a single application of fertiliser remain entrenched in the early reclamation phase for up to 25 years. / Forestry, Faculty of / Graduate

Traditionally, pre-contact Native Americans have served as a biological reference for identifying modern Asian individuals in aspects of the biological profile due to their distantly shared genetic history, although this assumption remains largely untested. This study explores the craniomorphic variability between Asian and Asian-derived groups to ascertain whether they can be differentiated using population-specific models. Cranial and mandibular nonmetric trait data were recorded on pre-contact Native Americans (n=150) and compared within a statistical framework to cranial trait data for modern Thai (n=150) and Japanese (n=150) individuals. Chi-square analyses indicate that the groups exhibit statistically significant differences in their trait expressions. Of the 35 traits analyzed, 31 differ significantly between the groups. Binary logistic regression equations for differentiating the Japanese, Thai, and Native Americans are presented, and cross-validated correct classification rates range 60.0-90.0%. Further, the inclusion of sex into the logistic regression equations failed to improve their accuracies. The results indicate that the Native American and Asian groups are not skeletally homogenous due to divergent population histories, and that numerous cranial and mandibular nonmetric traits are resolute enough to detect differences within and between Asian and Asian-derived groups. Thus, this study highlights the utility of nonmetric traits in identifying individuals beyond the traditional African (“Black”), (“White”), and Asian groupings.

I examined the effects of fire frequency on reptile and amphibian community composition in a periodically burned sandhill habitat in west-central Florida. Plots burned in 2003 had lower species richness, diversity, and evenness indices than plots that had not been burned during the previous six years. Community composition was different among plots burned at different times and followed a gradient of change that corresponded to the time since the last fire. Aspidoscelis sexlineata were the most abundant lizards in recently burned plots, while Scincella lateralis and Plestiodon inexpectatus were relatively more abundant in unburned plots. Gopherus polyphemus were least abundant in unburned plots. Community composition among plots was correlated with mean leaf litter cover and herbaceous ground cover. Mean percent cover of leaf litter and herbaceous vegetation responded to the time since the last fire. Fire indirectly affects community composition through changes in environmental variables, such as percent coverage of leaf litter and herbaceous vegetation.Additionally, I examined annual variation in reptile and amphibian community composition over a period of four years in the mid 1980s and again in 2004. Differences existed in number of individuals captured, diversity, and evenness among years. Community composition was different between all consecutive years except 1986 and 1987. Number of individuals captured per year and annual differences in community composition were correlated with summer rainfall. The annual pattern of variation in community composition over time was no different in unburned experimental plots than in experimentally burned treatments. The power to detect such a difference is low, however, because of low replication. No apparent loss of species occurred between the 1980s and 2004, but the abundances of two non-native species were significantly higher in 2004 than in the 1980s. A third exotic species was documented at the site for the first time in 2004. Because of the increasing number of exotic reptiles and amphibians in Florida, the site is at risk of invasions of other species.

Ce travail de thèse porte sur la variation exo- et endostructurale des couronnes des dents permanentes du maxillaire et de la mandibule chez les Néandertaliens et les Hommes modernes. Il intègre trois champs d’investigation faisant appel à l’imagerie a haute-résolution (e.g. μCT-scan) et a la 3eme dimension appliquées sur les canines, les prémolaires et les molaires. L’un de ces champs concerne l’épaisseur et la proportion de l’email 3D, le deuxième concerne la forme de la surface externe de l’email et de la jonction email-dentine (morphométrie géométrique 3D) et le dernier concerne les traits non métriques a la surface externe de l’email et à la jonction email-dentine. Cette étude comprend un échantillon microtomographique de 190 dents néandertaliennes, incluant les données inédites des dents de l’Hortus (Hérault, France) et du Portel-Ouest (Ariège, France) (n=45), et de 300 dents d’Hommes modernes. Les résultats montrent que l’approche 3D des couronnes dentaires au moyen de la microtomographie s’avère être très utile pour distinguer les Néandertaliens des Hommes modernes en termes d’épaisseur et de proportion d’email 3D pour la majorité des dents étudiées, a l’exception des M1/M1 et de la forme 3D du contour marginal de la JED de la majorité des dents. De plus, des différences dans l’organisation des tissus des couronnes ont été observées au sein de l’échantillon des Néandertaliens entre les spécimens de Krapina, pouvant être considères comme des premiers représentant de ce taxon (SIM5e, env. 130 ka) et ceux de l’Hortus et du Portel, pouvant être considérés comme des derniers représentants (SIM3, env. 44 ka) et dont l’organisation des tissus des couronnes dentaires se rapproche de celle des Hommes modernes. Cela suggère une importante variation intergroupe chez les Néandertaliens d’ordre géographique ou diachronique, lequel se traduirait par une réorganisation endostructurale des tissus coronaires en lien avec la réduction du volume absolu des couronnes dentaires. / This work focuses on the exo- and endostructural crowns variations of maxillary and mandible permanent teeth of Neandertals and modern Humans. It integrates three fields of investigation using high-resolution imaging (e.g. μCT-scan) and the third dimension applied in canines (excepted 3D geometric morphometrics), premolars and molars. One of these fields relates to the 3D enamel thickness and the 3D tissues proportion, the second concerns outer enamel surface and enamel-dentine junction shapes (3D geometric morphometrics), and the third concerns the nonmetric traits at the outer enamel surface and enamel-dentine junction. This study includes a sample of 190 microtomographic Neandertal teeth, including unpublished microtomographic data of Hortus (Hérault, France) and Portel-Ouest (Ariège, France) teeth (n = 45), and microtomographic sample of 300 modern Humans teeth. The results show that 3D approach of dental crowns using microtomography data is very useful to distinguish Neandertals from modern Humans in terms of 3D enamel thickness and tissues proportions (except for the M1 / M1), as well as in terms of the EDJ marginal edge shape, for the majority of studied teeth. Moreover, differences of tissues organization in crowns were observed within the Neandertal sample, between the Krapina specimens, that can be considered as a group of early Neandertals (MIS 5e, approx. 130 ka), and the Hortus and Portel specimens, that can be considered as a group of least Neandertals (MIS 3, approx. 44 ka) and show a tissues organization of dental crowns more similar to modern Humans. This suggests a significant geographic or diachronic inter-group variation among Neandertals that seems to relate endostructural reorganization of the coronary tissues to the reduction of the absolute volume of dental crowns.