Please read the abstract in the section 00front of this document / Dissertation (M Dietetics)--University of Pretoria, 1999. / Human Nutrition / unrestricted

In the present study the effect of diets restricted in either zinc or pyridoxine upon estrogen directed gene expression in the mature rat uterus was tested. Sexually mature female rats were maintained on zinc-adequate (40 mg/kg diet) <i>ad libitum</i> or restricted-fed, pyridoxine-deficient, or zinc-deficient ( < 1 mg/kg diet or 3mg/kg diet) <i>ad libitum</i>-fed diets for 35 days. All animals were bilaterally ovariectomized and used for experimentation at 14 days post ovariectomy. On day 35 each rat was injected intraperitoneally with estrogen. They were killed at different times post injection and thymidine kinase (TK, EC 2.7.1.21) or creatine kinase (CK, EC 2.7.3.2) activity was assayed in uteri cytosol fractions. In addition the steady state level of <i>ckb</i> mRNA in uteri cytosol fractions was measured following estrogen administration. The weight gain of the rats fed the low zinc and low pyridoxine diets was significantly lower than those fed the zinc-adequate diet ad libitum. The consumption of the zinc-deficient diet resulted in a significant decrease in plasma zinc while a pyridoxine deficient diet produced a significant reduction in plasma pyridoxine. Vehicle-injected uterine TK activity was 2-3 pmoles of d-TMP/min/mg protein. The TK activity was significantly increased (p < 0.05) 42 h post estrogen injection on the zinc-adequate diet <i>ad libitum</i> and pair-wt fed rats. This activity was sustained at 48 h post injection prior to declining to control values within 60 h. The maximum (4-fold) increase occurred at 36 h post estrogen injection in pyridoxine-deficient rats which was sustained at 42 & 48 h. The increase in uterine TK activity was 3-fold at 42 hand 48 h post injection. However this increase was not significantly different from the peak value seen in zinc-adequate and pyridoxine-deficient diet fed rats. No measurable effect of estrogen on CK activity was observed on the zinc adequate or zinc-deficient diet fed rats using a coupled enzyme assay. However the time course of ckb mRNA induction on the zinc-adequate pair-wt fed rats following estrogen administration paralleled the time course of estrogen induced protein (IP) synthesis previously observed by Gorski et al. (1970). IP is now known to be the brain type isoenzyme of creatine kinase. An induction of <i>ckb</i> mRNA between 0-3 h post estrogen injection was not observed on the zinc-deficient diet fed rats. However in a subsequent experiment an induction of uterine ckb mRNA 2 h following estrogen administration was observed in zinc-deficient rats. The possible reasons for this discrepancy are discussed. Zinc ions are known to be required to enable the estrogen receptor complex to bind to DNA and initiate transcription. It has been hypothesized that inadequate provision of dietary zinc may therefore reduce compliance to estrogen directed gene expression by limiting the efficiency of recruitment of zinc ions for stabilization of the zinc finger of the steroid receptor. The results of the present study failed to support this hypothesis at this moderate level of zinc depletion. / Ph. D.

Manipulation of dietary zinc produced a different body zinc status in male Japanese quail during 3 weeks of treating period. After intramuscular injection with 8μmol/100g body weight of estradiol-17β, Japanese quail were sacrificed and vitellogenin (phosphoprotein) production was assessed in these birds by analyzing plasma for protein-bound phosphorus concentrations (PBP). Plasma PBP concentrations of estrogen-injected male quails increased from undetectable values for control birds to O.66mg/ml for high-zinc birds and O.42mg/ml for low-zinc birds. About 36% loss of vitellogenin synthesis was associated with the consumption of the zinc-deficient diet. Plasma zinc concentrations also increased on the estrogen injection because of the zinc-binding property of vitellogenin. The extra zinc ions in the plasma were considered to be come from the body zinc pools. Liver as well as other major tissues was believed to act as a reservoir of zinc for egg development but it was not observed that liver was the major source of the extra zinc in the plasma. / Master of Science

The study aimed at determining the contribution of cowpeas to dietary diversity and food access, among women living in a peri-urban area of Gaborone called Gabane village in Botswana. Consuming a variety of food groups ensures adequate intake of nutrients for health. Diet diversity measures diet quality, food access and an indication of nutritious diets. Cowpeas are a nutritious vegetable which can contribute to diet diversity, diet quality and food access of many poor rural households who cannot afford to purchase a wide variety of nutritious food (ARC, 2006). Data was collected through administering a Cowpea consumption survey questionnaire which indicated that most of the households consume cowpeas which are essential to improve household food access, diet diversity and diet quality. A socio-demographic questionnaire was also used and it indicated an average number of household members of 6 people. The respondents were mainly the elderly people of 56 years and above with primary school education as the most achieved level of education. A Household Dietary Diversity Score (HDDS) questionnaire was contextualised for local foods consumed in Botswana. Focus group discussions were conducted to collect more data from women regarding their attitude, opinions and perceptions of consuming a variety of foods, cultivating and consuming cowpeas to ensure good health. The focus group results indicated that cowpeas were consumed by most families and 100% of adults and children consume cowpeas as leaves and seeds. Cereals were predominantly consumed by 95% of households, vitamin A rich fruits and vegetables by 52.5%, meat, poultry and fish by 42.5%. Vitamin A rich foods such as vegetables, fruits and organ meat were poorly consumed except green leafy vegetables which included cowpea leaves consumed by 52.5%. Meat, poultry and fish were consumed by 42.5%. Legumes, nuts, seeds and organ meat were also poorly consumed. vii Cowpeas grow well in Botswana, are Batswana’s cultural food, can improve nutritional intake and livelihoods through sold surplus profit. A diet diversity score of 2.9, well below the advised cut-off point of 4 was documented, indicating poor household dietary diversity and poor diet quality and food access. / Agricultural, Animal Health and Human Ecology / Thesis (M.A. (Human ecology) )

The study aimed at determining the contribution of cowpeas to dietary diversity and food access, among women living in a peri-urban area of Gaborone called Gabane village in Botswana. Consuming a variety of food groups ensures adequate intake of nutrients for health. Diet diversity measures diet quality, food access and an indication of nutritious diets. Cowpeas are a nutritious vegetable which can contribute to diet diversity, diet quality and food access of many poor rural households who cannot afford to purchase a wide variety of nutritious food (ARC, 2006). Data was collected through administering a Cowpea consumption survey questionnaire which indicated that most of the households consume cowpeas which are essential to improve household food access, diet diversity and diet quality. A socio-demographic questionnaire was also used and it indicated an average number of household members of 6 people. The respondents were mainly the elderly people of 56 years and above with primary school education as the most achieved level of education. A Household Dietary Diversity Score (HDDS) questionnaire was contextualised for local foods consumed in Botswana. Focus group discussions were conducted to collect more data from women regarding their attitude, opinions and perceptions of consuming a variety of foods, cultivating and consuming cowpeas to ensure good health. The focus group results indicated that cowpeas were consumed by most families and 100% of adults and children consume cowpeas as leaves and seeds. Cereals were predominantly consumed by 95% of households, vitamin A rich fruits and vegetables by 52.5%, meat, poultry and fish by 42.5%. Vitamin A rich foods such as vegetables, fruits and organ meat were poorly consumed except green leafy vegetables which included cowpea leaves consumed by 52.5%. Meat, poultry and fish were consumed by 42.5%. Legumes, nuts, seeds and organ meat were also poorly consumed. vii Cowpeas grow well in Botswana, are Batswana’s cultural food, can improve nutritional intake and livelihoods through sold surplus profit. A diet diversity score of 2.9, well below the advised cut-off point of 4 was documented, indicating poor household dietary diversity and poor diet quality and food access. / Agricultural, Animal Health and Human Ecology / Thesis (M.A. (Human ecology) )

M.Cur. / The intensive care nurse is lawfully required to maintain the nutrition of patients in the intensive care unit with which she is concerned, This includes the assessment of the nutritional status, planning and implementation of the nutritional regimen, as well as evaluation of the effect of nutritional support on the patient. Recording of this information is an important part of the abovementioned processes. Through the correct and scientific administering of nutritional support, as well as co-operation by the members of the nutritional support.team, aid the intensive care nurse in restoring, maintaining and promoting the patient's health. The intensive care nurse hereby facilitates the patient's aim to be healthy. The purpose of this study is: to determine the involvement of the nutritional support team with regard to the nutritional regimens of patients in surgical intensive care units; - to evaluate existing individual nutritional regimens of patients in the concerned intensive care units, and to establish guidelines for the nurse in intensive care units, regarding the administering of nutrition to the surgical patient. An exploratory-descriptive research design in questioning and retrospective auditing, is used. by means of one structured audit form. The results indicate the following: which use is made of Auditing has taken place - the involvement of the members of the nutritional support team, especially the dietician who should play an important role in the nutritional support of patients in surgical intensive care units, is inadequate; - the steps in the process of nutritional support, namely assessment of the nutritional status and planning of nutritional regimens achieved a low percentage of entirety. Implementation achieved a high percentage, but it was the complete execution of nutritional regimens that was inadequately planned. Furthermore the nurse's report regarding the evaluation of nutritional regimens and nutritional status was present, but incomplete and inaccurate...

Thesis (MNutr (Interdisciplinary Health Sciences. Human Nutrition))--University of Stellenbosch, 2006. / OBJECTIVE: The primary aim of the study was to determine the nutritional status of vegetarian and non-vegetarian students in relation to their dietary preferences and risk factors (dietary, physical inactivity and obesity) for CHD. DESIGN: Cross-sectional analytical study. Setting: The Adventist International Institute of Advanced Studies (AIIAS) situated in the province of Cavite, Philippines. METHODS: The sampling frame was all graduate students at AIIAS (n=203). Of these students 153 returned the distributed dietary questionnaires which determined dietary practices, thus yielding a stratified random sample of 70 registered students (≥20y and ≤50y) who met the inclusion criteria of the study. Three 24-hour recalls and a self-administered food frequency questionnaire assessed dietary practices. Lifestyle was assessed by means of questionnaires, which also included the socio-demographic characteristics of the subjects. Anthropometric measurements included height, weight and waist circumferences. RESULTS: Seventy subjects participated in the study [non-vegetarian (n=38) and vegetarian (n=32)]. The mean age of subjects was 33.3 [(SD) 1.6] and 38.4 (1.9) years for non-vegetarian and vegetarian males respectively, with the respective means for females being 35.7 (2.0) and 33.2 (2.1) years. The majority of the vegetarians’ income was insignificantly below $10.000 as compared with that of non-vegetarians’, in whom annual income earned was within the $10.000- $50.000 range per year. Variations in level of education between the dietary groups were small and inconsistent, most of whom were characterized by a high education level. Within this cohort, mean BMI and WC were insignificantly lower in the vegetarians when compared with the non-vegetarians. For males, the prevalence of overweight, pre-obese and obese (p>0.05) for non-vegetarians was insignificantly higher than vegetarians. Insignificantly, female vegetarians were more pre-obese than non-vegetarians. As far as waist circumference was concerned, the prevalence of subjects observed in the alerting (≥94cm) and action zone (≥102cm) (p≤0.05) was 21% and 4% for non-vegetarian males, while 0% and 6% for vegetarian males. For females, more vegetarians were insignificantly prevalent in the alerting zone (≥80cm) as compared to the nonvegetarians. Both dietary cohorts illustrated no considerable differences that exemplified moderate to a high level of physical activity. All subjects, regardless of dietary preference, were non-smokers and consumed no alcohol (p>0.05). Overall, mean daily nutrient intake met current recommendations and there was no statistically significant difference between the two cohorts, except for fat and saturated fatty acids (SFA), which was higher among the non-vegetarians. Carbohydrate and fiber consumption was greater in the vegetarians. According to the DRIs, there were no intakes above the UL, however inadequate intakes of calcium and zinc posed possible risk of deficiency for both dietary groups. CONCLUSIONS: A small percentage of subjects in both cohorts were at risk of CHD morbidity. Both groups followed good lifestyle habits with dietary choices being of greater concern among non-vegetarians.

No description available.

Indiana University-Purdue University Indianapolis (IUPUI) / Objectives: To determine (1) whether prescribed and delivered energy and protein intakes during the first two weeks of life met Ziegler’s estimated requirements for Very Low Birth Weight (VLBW) infants, (2) if actual energy during the first week of life correlated with time to regain birth weight and reach full enteral nutrition (EN) defined as 100 kcal/kg/day, (3) if growth velocity from time to reach full EN to 36 weeks’ postmenstrual age (PMA) met Ziegler’s estimated fetal growth velocity (16 g/kg/day), and (4) growth outcomes at 36 weeks’ PMA. Study design: Observational study of feeding, early nutrition and early growth of 40 VLBW infants ≤ 30 weeks GA at birth in three newborn intensive care units NICUs. Results: During the first week of life, the percentages of prescribed and delivered energy (69% [65 kcal/kg/day]) and protein (89% [3.1 g/kg/day]) were significantly less than theoretical estimated requirements. Delivered intakes were 15% less than prescribed because of numerous interruptions in delivery and medical complications. During the second week, the delivered intakes of energy (90% [86 kcal/kg/day]) and protein (102% [3.5 g/kg/day]) improved although the differences between prescribed and delivered were consistently 15%. Energy but not protein intake during the first week was significantly related to time to reach full EN. Neither energy nor protein intake significantly correlated with days to return to birth weight. The average growth velocity from the age that full EN was attained to 36 weeks’ PMA (15 g/kg/day) was significantly less than the theoretical estimated fetal growth velocity (16 g/kg/day) (p<0.03). A difference of 1 g/kg/day represents a total deficit of 42 - 54 grams over the course of a month. At 36 weeks’ PMA, 53% of the VLBW infants had extrauterine growth restriction, or EUGR (<10th percentile) on the Fenton growth grid and 34% had EUGR on the Lubchenco growth grid. Conclusions: The delivered nutrient intakes were consistently less than 15% of the prescribed intakes. Growth velocity between the age when full EN was achieved and 36 weeks’ PMA was 6.7% lower than Ziegler’s estimate. One-third to one-half of the infants have EUGR at 36 weeks’ PMA.

Indiana University-Purdue University Indianapolis (IUPUI) / Gastric residual volume (GRV) is a clinical assessment to evaluate gastric emptying and enteral feeding tolerance. Factors such as the tube size, tube material, tube port configuration, placement of the tube in the gastric fluid, the amount of fluid and person completing the assessment may influence the accuracy of residual volume assessment. Little attention has been paid to assessing the accuracy of GRV measurement when the actual volume being aspirated is known, and no studies have compared the accuracy in obtaining RV using the three different techniques reported in the literature that are used to obtain aspirate in practice (syringe, suction, and gravity drainage). This in vitro study evaluated three different methods for aspirating feeding formula through two different tube sizes (10 Fr [small] and 18 Fr [large]), tube materials (polyvinyl chloride and polyurethane), using four levels of nursing experience (student, novice, experienced and expert) blinded to the five fixed fluid volumes of feeding formula in a simulated stomach, to determine if the RV can be accurately obtained. The study design consisted of a 3x2x2x4x5 completely randomized factorial ANOVA (with a total of 240 cells) and 479 RV assessments were made by the four nurse participants. All three methods (syringe, suction and gravity) used to aspirate RV did not perform substantially well in aspirating fluid, and on average, the methods were able to aspirate about 50% of the volume available. The syringe and suction techniques were comparable and produced higher proportions of RVs, although the interrater reliability of RV assessment was better with the syringe method. The gravity technique generally performed poorly. Overall, the polyvinyl chloride material and smaller tubes were associated with higher RV assessments. RV assessment is a variable assessment and the three methods did not perform well in this in vitro study. These findings should be further explored and confirmed using larger samples. This knowledge will be important in establishing the best technique for assessing RV to maximize EN delivery in practice and will contribute to future research to test strategies to optimize EN intake in critically ill patients.