The aim of this study was to understand the cellular mechanisms in enterocytes, which may decrease glucose uptake by viscous oat β-glucan. An in-vitro cell model examined the effect of diffusion limitation, fluid shear stimulation, and increased intestinal stretching. Mechanical stimulation of IEC-6 was assessed. A Flexcell Cell Streamer device applied different fluid flow stresses on cells. Flexcell FX-4000 was used for biaxial stretching of the cells. Following the confirmation of appropriate use of the cell model, the results indicated that high viscosity oat β-glucan might provide a physical barrier limiting diffusion of nutrients to the cells apical surfaces. Western blot analysis confirmed weak mechanical stimulation on the cells. Mechanical stimulation did not influence glucose uptake. Strain-induced cells showed lower activities in their glucose uptake. In conclusion, there may be a significant contribution of direct effects of the viscosity of oat β-glucan on cellular mechanisms of uptake in enterocytes.

The viscous properties of oat beta-glucan in the gut are influenced not only by beta-glucan content, but also solubility and molecular weight. The objective of this study was to determine the effects of oat processing, genotype and growing location on the physicochemical properties of beta-glucan. Five oat genotypes (HiFi, Leggett, CDC Dancer, Marion and CDC Morrison) grown in two locations (Saskatoon and Kernen) were dehulled (untreated) and processed in a pilot facility through kilning (kilned/not flaked) and subsequent steaming and flaking (kilned/flaked). Three oat genotypes (HiFi, Leggett and Furlong) went through a commercial processing including dehulling, kilning and steaming and flaking. Both pilot and commercial scale processing, genotype and growing environment had significant effects on viscosity, molecular weight and solubility of beta-glucan. Results indicate that there is potential for processors to improve the physicochemical/nutritional properties of oat end products through processing of specific oat genotypes from selected growing locations.

Thesis (M.Agr.St.) - University of Queensland, 2005. / Includes bibliography.

Abstract Ecological based weed management strategies are imperative in cropping systems when herbicide use is limited or prohibited. Herbicides are not applicable in controlling wild oat (Avena fatua L.) in oat (Avena sativa L.) cropping systems, as they are closely related. Moreover, herbicide use is prohibited in organic oat cultivation, resulting in a need for developing alternative weed management strategies. Enhancing the crop competitive ability (CA) can be an essential strategy in managing weeds in such instances. Two studies were carried with the objectives to: 1) evaluate newly developed oat genotypes for their CA against wild oat; and 2) develop a competitive organic oat cropping system integrating mechanical and cultural weed control practices. In the first study, seven oat lines deliberately bred for enhanced CA and their two parental cultivars were evaluated for the CA with wild oat. The genotypes yielded similarly in the presence and in the absence of wild oat competition. The tall oat line SA050479 with greater seedling leaf size was more wild oat suppressive among all lines. Moreover, SA050479 had greater yield potential and grain quality; thus, it has the potential to be developed as a commercial wild oat suppressive cultivar. The second study used two contrasting levels of genotype, row spacing, crop density and a post-emergence harrowing and a non-harrowed control in two organic oat fields to develop an integrated weed management system. High crop density and harrowing increased the grain yield by 11% and 13% respectively. The competitive cultivar CDC Baler and high crop density (500 plants m-2) reduced weed biomass by 22% and 52% respectively. Harrowing reduced weed density by more than 50% in three site-years. The cultural and mechanical weed control practices when combined were additive in increasing grain yield and reducing weed biomass. Oat seed yields were increased by 25% when high crop density planting and harrowing were combined. Similarly, the combined effect of competitive cultivar, high crop density, and post-emergence harrowing were greater as weed biomass was reduced by 71%. The outcome of this project implies the importance of enhancing the crop CA by means of crop breeding and integrating cultural and mechanical weed control strategies. Furthermore, this study was able to identify the importance of ecological based weed management strategies in order to overcome the constraints in weed management in present oat cropping systems.

The inability to control wild oat (Avena fatua L.) in oat (Avena sativa L.) crops by chemical means limits growers to the use of cultural control methods. Delayed seeding is the most commonly used measure; however, both crop yield and quality may suffer as a result. The objectives of this research were to i) determine if western Canadian oat genotypes differ in competitive ability, ii) determine the effect of increased plant populations on oat wild oat competition, iii) determine the effect of wild oat competition on oat quality, and iv) establish whether or not oat genotype and seed size affect germination characteristics under low temperature and moisture stress. These objectives were tested using field and laboratory experiments. Morphologically diverse oat genotypes differed in their ability to both tolerate wild oat competition and interfere with wild oat growth. Although low yielding under weed-free conditions, when subject to wild oat competition CDC Bell was able to maintain yield, reduce wild oat seed production and was the most competitive of the genotypes examined. Increased plant populations achieved through higher seeding rates provide an effective means by which to enhance the competitive ability of oat genotypes resulting in reduced yield loss and wild oat seed production. With the exception of the percentage of wild oat seed in harvested oat samples, wild oat competition had minimal effect on oat quality. Differences in germination characteristics were observed among the genotypes examined. Conclusions that emerge from this research are that i) oat genotypes differ in their ability to tolerate and interfere with wild oat competition, ii) increased plant populations may provide a long-term control measure that may reduce weed seed contribution to the soil seedbank as well as enhance the competitive ability of oat, iii) wild oat competition has minimal effect on milling oat quality with the exception of percentage of wild oat seed in harvested samples and iv) that median germination time varies among oat genotypes.

The inability to control wild oat (Avena fatua L.) in oat (Avena sativa L.) crops by chemical means limits growers to the use of cultural control methods. Delayed seeding is the most commonly used measure; however, both crop yield and quality may suffer as a result. The objectives of this research were to i) determine if western Canadian oat genotypes differ in competitive ability, ii) determine the effect of increased plant populations on oat wild oat competition, iii) determine the effect of wild oat competition on oat quality, and iv) establish whether or not oat genotype and seed size affect germination characteristics under low temperature and moisture stress. These objectives were tested using field and laboratory experiments. Morphologically diverse oat genotypes differed in their ability to both tolerate wild oat competition and interfere with wild oat growth. Although low yielding under weed-free conditions, when subject to wild oat competition CDC Bell was able to maintain yield, reduce wild oat seed production and was the most competitive of the genotypes examined. Increased plant populations achieved through higher seeding rates provide an effective means by which to enhance the competitive ability of oat genotypes resulting in reduced yield loss and wild oat seed production. With the exception of the percentage of wild oat seed in harvested oat samples, wild oat competition had minimal effect on oat quality. Differences in germination characteristics were observed among the genotypes examined. Conclusions that emerge from this research are that i) oat genotypes differ in their ability to tolerate and interfere with wild oat competition, ii) increased plant populations may provide a long-term control measure that may reduce weed seed contribution to the soil seedbank as well as enhance the competitive ability of oat, iii) wild oat competition has minimal effect on milling oat quality with the exception of percentage of wild oat seed in harvested samples and iv) that median germination time varies among oat genotypes.

Abstract Ecological based weed management strategies are imperative in cropping systems when herbicide use is limited or prohibited. Herbicides are not applicable in controlling wild oat (Avena fatua L.) in oat (Avena sativa L.) cropping systems, as they are closely related. Moreover, herbicide use is prohibited in organic oat cultivation, resulting in a need for developing alternative weed management strategies. Enhancing the crop competitive ability (CA) can be an essential strategy in managing weeds in such instances. Two studies were carried with the objectives to: 1) evaluate newly developed oat genotypes for their CA against wild oat; and 2) develop a competitive organic oat cropping system integrating mechanical and cultural weed control practices. In the first study, seven oat lines deliberately bred for enhanced CA and their two parental cultivars were evaluated for the CA with wild oat. The genotypes yielded similarly in the presence and in the absence of wild oat competition. The tall oat line SA050479 with greater seedling leaf size was more wild oat suppressive among all lines. Moreover, SA050479 had greater yield potential and grain quality; thus, it has the potential to be developed as a commercial wild oat suppressive cultivar. The second study used two contrasting levels of genotype, row spacing, crop density and a post-emergence harrowing and a non-harrowed control in two organic oat fields to develop an integrated weed management system. High crop density and harrowing increased the grain yield by 11% and 13% respectively. The competitive cultivar CDC Baler and high crop density (500 plants m-2) reduced weed biomass by 22% and 52% respectively. Harrowing reduced weed density by more than 50% in three site-years. The cultural and mechanical weed control practices when combined were additive in increasing grain yield and reducing weed biomass. Oat seed yields were increased by 25% when high crop density planting and harrowing were combined. Similarly, the combined effect of competitive cultivar, high crop density, and post-emergence harrowing were greater as weed biomass was reduced by 71%. The outcome of this project implies the importance of enhancing the crop CA by means of crop breeding and integrating cultural and mechanical weed control strategies. Furthermore, this study was able to identify the importance of ecological based weed management strategies in order to overcome the constraints in weed management in present oat cropping systems.

The project was carried out in collaboration with Lantmännen with the aim to analyse the lipase activity in oat endosperm, non-heat-treated whole grain oat flour and heat-treated whole grain oat flour and to investigate whether lipase activity could be inactivated in oat endosperm by reducing water activity by various methods such as oven drying and freeze drying to extend oat shelf life. Lipase activity was measured by a wet extraction of the flour and an artificial substrate that gives a coloured product if lipase enzymes are active in the extract. The formation of the product was measured during five minutes at room temperature or 37 °C using a spectrophotometer, and the enzyme activity in units/g of flour was calculated. It was not possible to measure the enzyme activity in the flour directly. The obtained results for the different oat fractions indicated differences in lipase activity between the oat fractions. Lipase activity for oat endosperm was 0.183 U/g, lipase activity for non-heat-treated whole grain oat flour was 0.088 U/mg and the heat-treated whole grain oat flour had no lipase activity. The differences were statistically significant since the p-value was <0.0001. The results from the analysis that compared whether oven-drying and freeze-drying as methods had an effect on lipase activity indicated that oven drying had the greatest effect when assay was performed at room temperature. However, when the assay was performed at 37 °C the lipase activity in oven-dried and freeze-dried oat endosperm were similar to non- treated samples. This indicates that temperature has a great impact on the lipase activity. Thus, oven drying and freeze drying could not completely inactivate the lipase activity, but probably reduce it.

Thesis (PhD (Agric.) -- Stellenbosch University, 1984. / INTRODUCTION: The study of seed dormancy and germination has for centuries occupied the minds of agronomists, physiologists, brewers, bakers and, more recently, weed scientists. The agronomist requires that the seed that he sows will germinate rapidly and uniformly and produce a vigorous healthy seedling .. The physiologist is interested in the understanding of the basic processes involved at the molecular level, and the geneticist in the inheritance of the quiescent character of the seed. Brewers seek a seed that will retain its viability at least until the following crop is harvested but which will also, on imbibition, rapidly set in motion those processes that will convert starch into sugar. The baker is concerned with the baking quality of the seed and, as far as he is concerned~ the more dormant the seed the better, as this eliminates the problem of pre-harvest sprouting which is very detrimental to baking quality. The weed scientist seeks to encourage all weed seeds present in the soil to germinate simultaneously so as to enable him to destroy the weed population with one application of herbicide or a single cultivation.

Morpho logical, anatomical, and histochemical effects of S-ethyl dipropylthiocarbamate (EPTC) on Avena sativa var . overland were studied after treatment of seeds at various EPTC concentrations. The oat seed germination percentage was not affected by EPTC treatment. EPTC delayed initial root and coleoptile development at all concentrations used . Two or three days after treatment, however, the roots of seedlings treated with EPTC concentrations lower than 3 ppm grew at the same rate as the untreated seedlings and showed no abnormalities. Primary and adventitious root growth of seedlings treated with 3 ppm and higher was inhibited. Most of the adventitious roots remained in the radicle stage and failed to elongate. Bases of the roots became necrotic and were quite brittle . Formative effects were greater in the shoot than in the root. As result of treatment, coleoptiles became chlorotic, thickened, and were hard and brittle . The first true leaf of treated seedlings tended to adhere to the coleoptile and was broken as the coleoptile elongated . The complete shoot meristem of 12 ppm EPTC treated seedlings broke at the base . Young leaves formed within coleoptiles of treated seedlings oftain failed to emerge. His tochemica l studies s howed more carbohydrates in the coleoptiles of the EPTC treated oat seedlings than in those of the untreated seedlings . The lip1d-containing materials in the coleoptiles of the germinating oat seedlings disappeared from the coleoptiles of fully grm;n untreated oat seedlings , while they still persisted in the coleoptiles of 5-day-old EPTC treated oat seedlings. This suggests that EPTC might inhibit the breakdown and utili zation of stored foods .