The germination and emergence of a seedling, or seedling recruitment remains an essential process in the establishment of a plant. This establishment can be limited by the availability of microsites within the soil profile, or the availability of seed within a given area. Three field experiments were initiated in Saskatchewan, Canada to examine the relative effect of seed and microsite limitations on weed seedling recruitment. The first experiment examined the effect of landscape position as well as nitrogen (N) rate and tillage system (zero tillage vs. conventional tillage) on weed seedling recruitment from an indigenous weed population. Survey results indicated habitat differentiation of the weed population with wild oat and cleavers preferentially recruiting in the lower landscape positions, Russian thistle and Kochia in the upper landscape positions, while green foxtail recruited in high levels on all landscape positions. This suggested that different weed species have different microsite requirements for weed seedling recruitment across contrasting landscape positions. The second field experiment examined the effect of landscape position and moisture availability on weed seedling recruitment from an artificial hand-seeded weed seedbank. This experiment indicated that seed limitation remained a very important factor, but even when irrigated, total seedling recruitment did not reach maximum recruitment, indicating water was not the only limiting resource for weed seedling recruitment. Microsite limitations were greatest on the upper slope position for all species with green foxtail having the greatest overall recruitment of the species across all landscapes and moisture regimes. The third experiment examined the effect of tillage system and density on weed seedling recruitment of wild oat, green foxtail, and wild mustard. Again, weed seedling recruitment remained a function of both microsite and seed limitations as absolute recruitment values increased for each density examined in this experiment. The agronomic significance of microsite limitation was negligible as high weed population numbers occurred for the highest weed seeding densities. Overall, microsite limitations remained negligible in these experiments for arable agriculture with the main influence on weed seedling recruitment most often being seed limitation in the natural seedbank.

Two experiments were conducted to evaluate the effects of dietary inclusion level of a low lignin hull, high-oil groat (CDC SO-I) oat on the performance, carcass characteristics and rumen degradation characteristics of feedlot cattle. In the first trial, 200 crossbred steers (average weight of 427.3 ± 22.4 kg) were allocated to 20 pens. Five treatments, formulated by replacing barley with increasing levels of CDC SO-I oat (Barley grain:CDC SO-I oat ratios of 100:0; 75:25; 50:50; 25:75 and 0:100 ; DM basis) were used. Four pens were randomly allocated to each treatment diet. Over the entire study there was a linear decrease (P< 0.01) in DMI and ADG with increasing inclusion level of CDC SO-I oat, whereas feed efficiency (gain:feed) decreased (P= 0.03) quadratically. Days on feed also increased (P= 0.03) quadratically for the steers fed the higher levels of CDC SO-I oat. Increasing the inclusion level of CDC SO-I oat in the diet also decreased (P< 0.01) carcass weight, dressing percentage and grade fat linearly. However, there was no effect of treatment on rib eye area and lean yield percentage. There was no significant effect of treatment on marbling score. While the results of this trial point to a negative effect of CDC SO-I oat on finishing performance, there were minimal differences between cattle fed 100% barley as the concentrate versus those fed 75% barley: 25% oat blend.<p> Trial 2 involved a metabolism trial to determine the effect of CDC SO-I oat inclusion level on rumen fermentation parameters of 5 fistulated heifers fed the same diets used in Trial 1. A 5 × 5 Latin square experiment design was used. Rumen degradation parameters (rumen pH, VFA, osmolality and ammonia nitrogen levels) and feeding behavior (time spent eating, ruminating, chewing and drinking) were measured. Mean rumen pH for the barley-based diet was 5.88 which was not different (P> 0.05) than the mean pH of 5.5 for the oat-fed cattle. Treatment did not affect (P> 0.05) time spent below pH cutoff values of 5.8, 5.5 and 5.2. No effect of oat inclusion level (P> 0.05) was observed on total VFA levels, molar proportion of individual fatty acids and osmolality while isobutyrate (P= 0.05) and ruminal ammonia nitrogen concentrations decreased linearly (P= 0.02) with the higher inclusion of CDC SO-I oat. Time spent eating was linearly (P< 0.01) increased with higher inclusion level of CDC SO-I oat.<p> Over all, the results of this study indicate that the replacement of barley by CDC SO-I oat in finishing diets decreases dry matter intake and as a result leads to reduced ADG, increased days on feed and lower slaughter and carcass weights. The reduced performance might be the result of higher fat content, high hull and/or faster degradation rate of oat starch leading to subacute ruminal acidosis in cattle fed higher levels of oat. However, replacing barley with CDC SO-I oat does not significantly change the rumen environment. The results of this study indicate that CDC SO-I oat can be successfully included up to a maximum level of 25% without any adverse effect on performance and carcass characteristics in the diets of finishing cattle.

No description available.

A series of experiments were conducted to investigate the nutritional value of a new oat variety developed by the Crop Development Centre at the University of Saskatchewan. Trials 1 and 2 evaluated performance of steers fed a low lignin hull, high oil groat (LLH-HOG) oat as a replacement for barley or corn. In trial 1, 400 steers were fed one of two diets with barley or the LLH-HOG oat at 37.8% of the diet DM. Dry matter intake was lower (P=0.02) and gain to feed improved (P0.01) for steers fed the oat-based diet. In trial 2, 240 steers were finished diets with barley, corn or the LLH-HOG oat at 88.2% of the finishing diet (DM). During finishing, steers on the oat diet had lower (P0.01) ADG, body and carcass (P<0.01) weights than barley or corn-fed cattle reflecting lower (P0.01) DMI. In trial 3, 20 steers were fed one of seven diets consisting of barley silage and 0, 28, 56, or 84% LLH-HOG oat or barley grain (DM basis) to compare nutrient digestibility. Apparent DM, OM, ADF and NDF digestibility coefficients were lower (P<0.05) for LLH-HOG oat-based diets compared to barley-based diets. Apparent CP and EE digestibility coefficients were higher (P<0.05) for the LLH-HOG oat diets. Trial 4 was conducted to assess ruminal fermentation differences between LLH-HOG oat- or barley-based finishing diets using four rumen cannulated steers. No diet effects (P>0.05) were observed for total ruminal VFA concentration or molar proportions of individual VFA however mean ruminal pH was lower (P=0.01) for steers fed the LLH-HOG oat-finishing diet. Further, the extent of pH decline in oat-fed cattle was greater (P<0.01) than for barley-fed cattle. The results indicate that the energy value of the LLH-HOG oat is equivalent or superior to that of barley for growing cattle. However, further research is required to identify factors limiting feed intake of cattle fed this new oat type in finishing diets.

This thesis research started with analysis of oat fatty acids by using three different transmethylation methods. Basic sodium methoxide was compared with traditional acidic methanol for the total fatty acid analysis, while diazomethane was used to analyze free fatty acids. Epoxy FAs were readily hydrolyzed to dihydroxy fatty acids under the acidic condition, which suggest an overestimation of hydroxyl fatty acids and underestimation of epoxy fatty acids in previous analyses. The sodium methoxide method proved more reliable to quantify the oat seed fatty acid composition. CDC Dancer oat seed analyzed here was comprised mostly of palmitic acid (PA), oleic acid (OA) and the polyunsaturated fatty acid (PUFA) linoleic acid (LA) in quantities of 23%, 32%, and 37% of total seed FA, respectively. As well, the seed contained small quantities of another PUFA, α-linolenic (ALA) and several unusual oxygenated fatty acids (UFAs), Δ15-hydroxy fatty acid (15HFA) and epoxy fatty acids in quantities of 0.85%, 0.68%, and 2.3%, respectively. This thesis further aimed to identify and assemble all FAD2-like genes from an oat Expressed-Sequence Tag (EST) database using FAD2 and FAD2-like proteins from other organisms as query sequences in order to clone all putative FAD2-like genes-of-interest (GOIs) from oat. From the contig assemblies of retrieved oat ESTs, four distinct, putative genes were identified. From the Δ12-desaturase (FAD2) queries, a putative FAD2-like (AsFAD2) gene was identified; the Δ15-desaturase (FAD3) queries revealed two putative oat FAD3-like (AsFAD3-1 and AsFAD3-2) genes, while an ω-3 desaturase (FAD7) query identified a fourth putative full-length FAD6-like coding sequence of two possible lengths, AsFADX and AsFADX+. The GOIs were then subcloned into a yeast expression vector and functionally characterized. AsFAD2a and AsFAD2b both demonstrated Δ12 desaturation on 18:1-9c substrate. AsFAD3-1 had no activity on any substrates present, while AsFAD3-2 exhibited weak Δ15-desaturation activity specifically on 18:2-9c,12c. Finally, AsFADX converted 18:1-9c to 18:2-9c,12c, while AsFADX+ had no activity. Then, a comparative analysis of transcript levels of these GOIs via quantitative real-time PCR (qRT-PCR) was performed across oat germinating seed, root, leaf, and developing seed. AsFAD2 transcript abundance was generally much higher than AsFAD3-1 and AsFAD3-2 in all tissues. AsFAD3-1 mRNA level was highest in developing seed tissue, slightly lower in leaf tissue, and lowest in root. AsFAD3-2 mRNA was highest in germinating seed, and lowest in leaf tissue. In summary, the data produced from this thesis could be used to enhance breeding efforts for establishing oat cultivars with healthier oil content.

The goal of the project was to enhance the period of weed growth prior to seeding in order to reduce weed emergence and weed competition after the crop has been planted. Weed growth was stimulated using either light tillage or by applying nitrogen fertilizer early in the spring. Light disturbance significantly increased pre-seed weed emergence while early applied nitrogen did not appear to have an effect. Post seeding weed emergence levels and weed biomass were similar among the light tillage and early nitrogen treatments. Therefore the goal of decreasing weed competition after seeding was not attained. Future research should focus on long-term strategies to reduce weed populations in field rather than seasonal strategies.

The goal of the project was to enhance the period of weed growth prior to seeding in order to reduce weed emergence and weed competition after the crop has been planted. Weed growth was stimulated using either light tillage or by applying nitrogen fertilizer early in the spring. Light disturbance significantly increased pre-seed weed emergence while early applied nitrogen did not appear to have an effect. Post seeding weed emergence levels and weed biomass were similar among the light tillage and early nitrogen treatments. Therefore the goal of decreasing weed competition after seeding was not attained. Future research should focus on long-term strategies to reduce weed populations in field rather than seasonal strategies.

Integrating cultural weed management practices with herbicides is an important strategy to reduce wild oat (Avena fatua L.) populations in Alberta, Canada. The purpose of this thesis is to expand the knowledge on wild oat seed banks and seedling emergence within integrated weed management systems. Field experiments were conducted from 2006-2007 to examine the impact of crop rotation, barley cultivar, barley seeding rate and herbicide rate on wild oat seed bank density, seed mortality, seedling emergence and seedling survivorship. Management systems consisting of diverse crop rotations, tall barley cultivars and increased barley seeding rates reduced wild oat seed bank density, seedling emergence and seedling survivorship, especially at low herbicide rates. Wild oat seed banks predicted subsequent wild oat emergence, whereas biomass and emergence strongly predicted seed banks. Lastly, 50% wild oat emergence was achieved at 537 and 509 growing degree days in 2006 and 2007, respectively, in Lacombe, Alberta. / Plant Science

Bibliography: leaves 128-146. / x, 146 leaves : ill. (some col.), maps ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Study was undertaken to determine the occurence and species incidence of wild oats in a major cropping region of southern Australia. Population dynamic studies were undertaken at two sites to define the seed bank decline and emergence pattern of several wild oat populations over a three year period. Management studies were conducted to determine appropriate strategies for the control of wild oats in southern Australian farming systems. / Thesis (Ph.D.)--Dept. of Agronomy and Farming Systems, The University of Adelaide, Roseworthy Campus, 1998

A estacionalidade de produção de plantas forrageiras é um problema muito frequente no Sul do Brasil, devido principalmente às baixas temperaturas e geadas que ocorrem no período de inverno. Essas características limitam o crescimento das plantas, determinando períodos de falta de forragem para os rebanhos. O trabalho foi realizado no período de março a novembro de 2009 com objetivo de avaliar as características produtivas de cultivares de forrageiras anuais de inverno (Avena strigosa Schereb, Lolium multiflorum Lam, Avena sativa, Triticum aestivum, Secale cereale e Triticosecale Wittmack) em quatro épocas de semeadura (11 de março, 08 de abril, 06 de maio e 03 de junho). O delineamento experimental empregado foi o de blocos ao acaso, com três repetições em parcelas subdivididas. Foram avaliados o número de dias para o primeiro corte, o número de cortes, dias de utilização da pastagem, a densidade de plantas e perfilhos, a produção total, a matéria seca residual, a produção por corte e a dinâmica produtiva (taxa de acúmulo diário mensal de MS). Houve interação significativa entre forrageiras e épocas de semeadura para todas as variáveis analisadas, indicando muitas combinações entre espécies forrageiras e data de semeadura de acordo com cada sistema. As aveias brancas, os azevéns, e a aveia preta IAPAR 61, apresentaram alta capacidade de produção e distribuição de forragem, especialmente se semeadas até início de maio. Para a variável taxa de acúmulo tiveram destaque às aveias brancas UTF Iguaçú e IPR 126, aveia preta IAPAR 61, os azevéns, e centeio, que presentaram em média produção diária de forragem de 51,4 kg ha-1 após a primeira utilização, demonstrando possuírem elevada capacidade de produção e manutenção da produção, também se semeadas até início de maio. / Forage production seasonality is a great concern in southwestern Brazil. It occurs mainly due to the low temperatures and frost that occur during the winter. Those characteristics prevent forage plants growth, causing lack forage shortage to herds. The trial was carried out from March to November 2009 in order to evaluate productive characteristics of winter annual forage grasses (Avena strigosa Schereb, Lolium multiflorum Lam, Avena sativa, Triticum aestivum, Secale cereale, and X Triticosecale Wittmack) under four sowing dates: March 11th, April 8th, May 6th and June 3rd. Completely randomized blocks with three replications in a split plot design was used. It was evaluated number of days to first harvest, number of harvests, pasture utilization period, tiller and plant population densities, total herbage production, stubble mass, herbage production by harvest, and production dynamics (dry matter accumulation rate). There was significant interaction between grasses and sowing dates for all variables, indicating many combinations between forage species and sowing date according each system. White oats, ryegrasses and black oat cv. IAPAR 61 performed better in relation to forage production and forage production persistance, especially if sown until early May. In relation to accumulation rate, white oats UTF Iguaçú and IPR 126, black oat IAPAR 61, ryegrasses, and rye presented better performance, which presented average of 51.4 kg DM ha-1 after first cut, showing a great performance in terms of forage production and maintenance of their forage production, especially when sowed until beginning May.