This report describes the origin and purpose of the Ministry of education document: Program Outline and Policy as it applies to OAC Biology. It provides analysis of components of the Program Outline and Policy with recommendations for improvement. Evaluation of students and characteristics of graduating students are discussed with recommendations. Difficulties with the OAC Biology guideline are presented with recommendations. A course outline which can be used by students is included. / None / Master of Science (Teaching)

No description available.

This project is designed to provide a teachable curriculum along with supporting materials for the OAC Geography - World Issues course, as it is taught at present in the academic stream of Ontario's secondary schools. Discussion of guideline requirements and disciplinary matters are also included. The topic is the infectious disease Acquired Deficiency Syndrome (AIDS). / Thesis / Master of Arts in Teaching (MAT)

This thesis is the first report linking differential distribution of cadmium (Cd) among tissues of hydroponically-grown soybean with Cd amendments – lower seed Cd-accumulating OAC Champion and higher seed Cd-accumulating OAC Bayfield – and the mechanisms responsible for these differences. OAC Champion retains 94% (64% for OAC Bayfield) of accumulated Cd in the root stock and Cd in its xylem sap is eleven-fold less concentrated than OAC Bayfield by seed fill. Though the movement of Cd to shoots is more restricted in OAC Champion, the concentration in some seed still approximates or exceeds 0.1 mg Cd • kg-1, indicating that in soils with elevated available Cd, there is potential for many cultivars of soybean to exceed the new suggested maximum for soybean, under an amendment to regulation (EC) No 1881/2006. Phytic acid was also assayed in seed tissue and OAC Bayfield was found to contain the highest concentrations.

Racemic metyhl 3-cyclohexene-1-carboxylate was resolved via enzymatic hydrolysis to afford the enantiomerically enriched 3-cyclohexene-1-carboxylic acid with PLE (S-configuration), HLE (S-configuration), CCL (S-configuration) and PPL (R-configuration) . The nucleoside&amp / #65533 / s precursor, 5-(hydroxymethyl)-2-cyclohexen-1-ol (19), was synthesized by iodolactonization, followed by iodine elimination and the reduction of the lactone. In connection with this work, alpha,beta-unsaturated and saturated cyclic ketones were selectively oxidized on alpha&#039 / - and alpha-positions using Mn(OAc)3 and Pb(OAc)4, respectively. The resultant racemic alpha&#039 / - and alpha-acetoxylated substrates were resolved into corresponding enantiomerically enriched alpha&#039 / - and alpha-hydroxylated and acetoxylated compounds via PLE hydrolysis.

ABSTRACT SYNTHESIS AND ENZYMATIC RESOLUTION OF VARIOUS CYCLOPENTENOID AND CYCLOHEXENOID TYPE COMPOUNDS iyig&uuml / n, &Ccedil / igdem Ph. D., Department of Chemistry Supervisor: Prof. Dr. Cihangir Tanyeli June 2005, 174 pages The aim of this thesis is to synthesize enantiomerically enriched cyclopentenoid and cyclohexenoid type of compounds with quaternary carbon stereocenters that are the simplest precursors of the complex natural products. The first part of the study involves the preparation of &amp / #945 / &#039 / -acetoxy &amp / #945 / &#039 / -substituted &amp / #945 / ,&amp / #946 / -unsaturated cyclic ketones. Methylation, ethylation, benzylation and allylation of cyclohexenone and cyclopentenone derivatives are performed. Then, these compounds are regioselectively oxidized at the &amp / #945 / &#039 / -position by Mn(OAc)3. This is the first successful example in the literature that &amp / #945 / &#039 / -tert-position is oxidized by Mn(OAc)3. The oxidations were also performed by Pb(OAc)4 and both methods were compared. Related to this study, in the second part, the enantiomeric resolution of the acetoxy derivatives were performed by various hydrolases. This study is the first example where the hydrolases are used to resolve tertiary positions. Among the enzymes used, Candida cylindracea lipase (CCL) showed the best enantioselectivity.

Oesophageal adenocarcinoma (OAC) is a leading cause of cancer death in the UK and current systemic therapies are ineffective for the majority of patients. The central aim of this work was to explore strategies to identify novel therapeutic targets. Research has failed, thus far, to identify a dominant oncogene in OAC, although the tumour suppressor p53 is frequently mutated. Inhibiting the mitotic kinase, polo-like kinase 1 (PLK-1), was proposed as a synthetic lethal strategy. PLK-1 was demonstrated to be over-expressed in both verified OAC cell lines and human OAC tissue compared to non-transformed cells and epithelium. Mutation of p53 was associated with over-expression of PLK-1 in both OAC and ovarian cancer tissue. Using a carefully validated viability assay, both an established and novel PLK-1 inhibitor were demonstrated to induce a G2/M arrest and reduce OAC cell proliferation. Relative selectivity was demonstrated for OAC compared to non-transformed cells. This therapeutic window could be enhanced with the induction of cancer cell cytotoxicity by pulsed administration of a short half-life inhibitor. Immunotherapeutics offer potential tumour-selectivity but no OAC-specific proteins have been defined. A comparative proteomic approach was employed to identify OAC-specific proteins as potential therapeutic targets. A tissue resource was established and methods to lyse fresh frozen biopsies optimised. An isobaric quantitative proteomic workflow was applied to OAC and matched normal biopsies and quantitative accuracy confirmed for 6 candidate proteins by immunohistochemistry. Proteome coverage and quantitative dynamic range were compared between isobaric and label-free systematic sequencing proteomic strategies applied to further patients’ tissues. The challenges of combining incomplete datasets were approached with a Bayesian framework to estimate the probability that a protein was missed during an experiment compared to not being present in the sample. This method was applied to generate a complete set of protein identifications and relative tissue expression. To gain insight into the dysregulated cellular processes in human OAC tissue, a network analysis was applied to the quantitative proteomic data. Enriched functional clusters were identified suggesting deranged glucose metabolism, potentially due to the Warburg effect. These findings were duplicated and candidate tumour-specific proteins identified in a further set of biopsies using the optimised quantitative proteomic method. The combined quantitative oesophageal proteomic dataset represents the largest in OAC to date. This thesis demonstrates a hypothesis-driven, synthetic lethal approach can yield cancer-selective therapeutic effects. Novel candidate therapeutic targets are also revealed through the development of quantitative proteomic methods and the application of network analysis.

Doctor of Philosophy / Department of Horticulture, Forestry, and Recreation Resources / Rhonda R. Janke / The goal of this study is to clarify the influence of organic fertilizer sources on vegetable crop yield under different production systems. This research hypothesized that organic soil amendments will produce healthy and vigorous plants with similar or higher yields while improving soil organic matter levels compared to conventional amendments. Applying organic fertilizer sources can be cost-prohibitive; moreover, synchronizing timing of crop nitrogen demand with soil plant available nitrogen is essential to maximizing yield and reducing nitrogen pollution to the environment. The objectives of this study are to evaluate yield in relation to soil fertility status at different fertility rates for organic and conventional management in field and high tunnel production systems, to measure plant nutrient status in crop petioles and compare it to available mineral N levels in soil at different growing stages, and to determine the effect of nitrogen availability of organic compared to conventional fertilization on plant available nitrogen and crop yield under both systems. A latin square experimental design was conducted from 2008 to 2010 at Kansas State University Research Center in Olathe KS to evaluate an organically managed vegetable rotation of tomato (Solanum lycopersicum L. ‘Bush Celebrity’) and pac choi (Brassica rapa L. ‘Mei Qing’) under three fertility rates; control, low (composted poultry manure), and high (composted poultry manure and fish hydrolyzate) in contrast with conventionally managed soils under two production systems (field and high tunnel). The effect of these four contrasting systems was measured on plant and soil nutrient status. All plots had cover crops of rye during the winter and buckwheat in the summer between pac choi crops. Soil nitrate-N (NO₃-N) and ammonium-N (NH₄-N) were measured, as well as petiole sap nitrate (NO₃⁻). In tomato, additional soluble fertilizers had no direct effect on yield in both field and high tunnel. Compost application had a positive effect on organic matter. In pac choi, additional liquid fertilizer helped organic field plots obtain maximum yield. Soil mineral nitrogen were affected by production system and fertility source, but statistical significance varied by crop and stage. Petiole sap reflected treatment regimens but not necessarily soil N status at each plant stage. The study also addressed long term management practices on organic and conventional available nitrogen. An incubation study on the soil at the conclusion of the field experiment explored the relationship between N mineralization from potentially mineralizable nitrogen (PMN) compared to Illinois Soil Nitrogen Test (ISNT) in control and pre-plant application fertility treatment for both field and high tunnel systems. The results indicated that ISNT concentration values for all soils were below the proposed value for corn crop suggested by (Khan, 2001). ISNT correlated with PMN with the stronger correlation being in field plots. ISNT also correlated with OM in field. Fertility rate showed a significant effect on total carbon and total nitrogen in organic systems of both field and high tunnel plots. This study supports composted poultry manure to improve the fertility status of the soil and to obtain a yield equal to that of conventionally managed soil.

The objective of this thesis was to investigate the genomic changes that have occurred due to the effects of long-term artificial selection applied by soybean breeders. A total of 42 cultivars from six different breeding programs, comprising the multi-generational pedigree of OAC Bayfield were genotyped with molecular markers and chromosomal inheritance was tracked throughout the pedigree. The graphical genotype profile of the 20 chromosomes revealed substantial allelic structure that has been built up in certain chromosomes, in the form of specific linkage blocks, which have been conservatively inherited. A selective sweep analysis using microsatellite markers was performed using the members of OAC Bayfield’s pedigree to identify genomic regions that have retained a molecular selective signature through OAC Bayfield in the varieties derived from it. Overall, there was a high level of agreement between the identified quantitative trait loci (QTL) and the phenotypic traits that would have been expected to be under breeders’ selection.

No description available.