Thesis (MScEng)--Stellenbosch University, 2011. / ENGLISH ABSTRACT: Nystagmus is an eye movement pattern that consists of a drifting gaze component, known as the slow phase, followed by a corrective quick phase component. The presence of nystagmus or the lack thereof under certain conditions can be used for various diagnostic purposes including the diagnosis of physiological, pathological and neurological conditions. The angular velocity of the quick phase can make the detection of nystagmus a challenging task for the untrained eye, since the quick phases are usually comparable with saccadic eye motions. The goal is thus to develop a fully automated diagnostic tool that can identify the presents of nystagmus in a patient’s eye motions. In this thesis, an appropriate eye tracking method was selected from a number of eye tracking methods that are commonly implemented in the literature. A video-oculography goggle concept was chosen based on criteria such as invasiveness, sampling rate, accuracy and telemedicine capability, amongst other nystagmus related necessities. A binocular video-oculography concept was chosen that satisfied the technical requirements and provided a cost-effective design. An automated analysis algorithm was developed for automatic nystagmus identification from eye motion data. The algorithm was validated by testing the performance of the algorithm on an optokinetic nystagmus signal. It proved to provide a reliable automatic identification of nystagmus beats, even in signals that contained nystagmus as well as random motion components. A statistical analysis showed that the algorithm provided a sensitivity of 91.8% and a specificity of 96.5% for pure nystagmus signals, and a sensitivity and specificity of 87.8% and 91.1% respectively for mixed signals. / AFRIKAANSE OPSOMMING: Nystagmus is ’n oogbewegingspatroon wat bestaan uit ’n dwalende tuurkomponent, wat die stadige fase genoem word, gevolg deur ’n vinnige korrigereringsbeweging wat bekend staan as die vinnige fase. Die teenwoordigheid van nystagmus, of afwesigheid daarvan in sekere gevalle, kan gebruik word in ’n verskeidenheid diagnostiese toepassings, onder andere die diagnose van fisiologiese-, patalogiese- en neurologiese kwale. Die hoeksnelheid van die vinnige fase lei daartoe dat nystagmus dikwels moeilik is om te bespeur vir ongeöefende oë, aangesien dit vergelykbaar is met saccade bewegings. Die doel van hierdie navorsing is dus die ontwikkeling van ’n stelsel wat ’n volledige automatiese identifisering van nystagmus kan behartig. ’n Gepaste oogvolgtegniek was gekies vanuit ’n aantal verskillende oogvolgmetodes wat dikwels in die praktyk gebruik word. Die finale keuse was ’n skermbril, video-oogvolgmetode wat gekies was op grond van kriteria soos onder andere, invallendheid, meetfrekwensie, akkuraatheid en geskiktheid vir telemedisyne toepassings. Die ontwikkelde brilkonsep bied ’n koste-effektiewe oplossing, met die moontlikheid om albei oë te volg en bevredig al die bogenoemde tegniese spesifikasies. ’n Geoutomatiseerde nystagmus identifiseringsalgoritme is ontwikkel. Die algoritme se effektiwiteit is getoets op optokinetiese nystagmusseine. Betroubare resultate is vekry vanaf die algoritme, selfs in die geval van gemengde seine wat bestaan uit arbritrêre- en nystagmus komponente. Statistiese analiese het gewys dat die algoritme ’n sensitiwiteit van 91.8% en ’n spesifisiteit van 96.5% kon behaal vir seine met slegs nystagmus inhoud. Vir gemengde inhoud seine het die algoritme ’n sensitiwiteit van 87.8% en spesifisiteit van 91.1% behaal.

Electro-oculography signals are corneo-retinal potentials that carry informationpertaining to eye movements. This information can be used to estimate drowsinesslevel of the subject which could provide interesting insights into research of acci-dent prevention. Of all features present, blink duration has been proved to be aneffective measure of drowsiness. The aim of this thesis work is to build a portablesystem to acquire and analyze electro-oculographic (EOG) signals in real-time.The system contains two sub-systems; a hardware sub-system that consists of thefilters, amplifiers, data acquisition card and isolation and the software sub-systemthat contains the program to acquire and analyze the signal and present the resultsto the observer. The filters were designed starting with simulation, implementa-tion on the prototype board, culminating in the design of a printed circuit board(PCB) and packaging. The complete software was written in PythonTMusing sev-eral relevant libraries for data processing. A text-based user interface was createdto enable easy user interaction. The results are graphically displayed in real-time. Ex-situ tests were done with two volunteers while in-situ test was done onone subject. The data from the in-situ tests showed "good signal quality" in a"noisy" environment concurring with the design specifications. To motivate theimportance of calibration, two calibration paradigms were used during ex-situtests, where one paradigm records only normal blinks while the other records longblinks and the results showed differences in detection and error rates. The obser-vations made from performance tests at various levels gave "satisfactory results"and proved the usefulness of the system for experimental purposes in-situ.

Le récepteur aux hydrocarbures aromatiques (AhR) est un facteur de transcription de la famille bHLH/PAS, activé par différents ligands exogènes dont les hydrocarbures aromatiques polycycliques ou halogénés (dioxines). A ce titre, il est décrit historiquement comme un récepteur de xénobiotiques dont le principal rôle est l’élimination de ces composés via la régulation des enzymes du métabolisme des xénobiotiques. Toutefois, des études récentes menées à l’aide de modèles souris invalidées pour le AhR, suggèrent indirectement que cette protéine régule des fonctions endogènes, notamment dans le système nerveux de mammifères dans lequel aucun rôle du AhR n’a jusqu’à présent été démontré. Nous avons donc utilisé le modèle de souris C57BL/6J AhR-/- pour mener à la fois des études comportementales et mécanistiques afin de déterminer ce rôle. Tout d’abord, nous avons identifié un défaut oculomoteur chez les souris AhR-/-, caractérisé par des mouvements spontanés horizontaux. En étudiant l’ensemble des circuits neurosensoriels potentiellement impliqués dans ce nystagmus pendulaire, nous avons montré que son origine est liée à des déficits du système visuo-moteur. De plus, en caractérisant et comparant les profils d’expression génique des cervelets de souris AhR+/+ et AhR-/- traitées ou non par de la 2,3,7,8 TétraChloroDibenzo-p-Dioxine (TCDD), nous avons montré que ce polluant, ligand du AhR, perturbait les fonctions endogènes du récepteur. Cet effet de « perturbation endogène » a été retrouvé dans un autre organe et est associé à une toxicité (fibrose hépatique). Cette étude a permis d’identifier de nouvelles fonctions physiologiques du AhR dans le système nerveux des souris, de caractériser un nouveau modèle animal de nystagmus pendulaire et ouvre de nouvelles perspectives de travail en neurotoxicologie. / The AhR is a basic helix-loop-helix Per/ARNT/Sim family (bHLH-PAS) transcription factor which is activated by many diverse compounds including polyphenols and aromatic hydrocarbons such as 2,3,7,8 TétraChloroDibenzo-p-Dioxin (TCDD). Initially, the AhR was described as a ubiquitous xenobiotic-activated transcription factor which promotes the elimination of xenobiotics by regulating the expression of genes involved in xenobiotic metabolism. However, mouse AhR knockout models have demonstrated that the AhR also regulates other normal physiological functions. In particular, functioning of the nervous system of mammals, previously unexplored in this respect, might depend upon the activity of the AhR. We, thus, performed behavioral and gene expression studies in AhR-/- mice to discover these functions. We, first, found that AhR-/- mice exhibit an oculomotor deficit which is characterized by spontaneous horizontal pendular eye movements that are probably due to a deficit in the visuo-motor circuitry. Second, we found that the cerebellar gene expression profiles of AhR-/- as compared to AhR+/+ mice resembled those of AhR+/+ mice treated with TCDD (the ligand with the highest affinity for the AhR). This suggests that TCDD disrupts some normal physiological functions of the AhR in the nervous system. Third, AhR-/- mice and AhR+/+ treated with TCDD both develop liver fibrosis. This further suggests a role for the AhR in normal liver function. In conclusion, this study reveals new physiological functions for the AhR in the mouse nervous system and describes a new model of pendular nystagmus. Moreover, the results also provide novel research perspectives in the field of neurotoxicology.

The electro-oculogram (EOG) is the most widely used technique for recording eye movements in clinical settings. It is inexpensive, practical, and non-invasive. Use of EOG is usually restricted to horizontal recordings as vertical EOG contains eyelid artefact (Oster & Stern, 1980) and blinks. The ability to analyse two dimensional (2D) eye movements may provide additional diagnostic information on pathologies, and further insights into the nature of brain functioning. Simultaneous recording of both horizontal and vertical EOG also introduces other difficulties into calibration of the eye movements, such as different gains in the two signals, and misalignment of electrodes producing crosstalk. These transformations of the signals create problems in relating the two dimensional EOG to actual rotations of the eyes. The application of an artificial neural network (ANN) that could map 2D recordings into 2D eye positions would overcome this problem and improve the utility of EOG. To determine whether ANNs are capable of correctly calibrating the saccadic eye movement data from 2D EOG (i.e. performing the necessary inverse transformation), the ANNs were first tested on data generated from mathematical models of saccadic eye movements. Multi-layer perceptrons (MLPs) with non-linear activation functions and trained with back propagation proved to be capable of calibrating simulated EOG data to a mean accuracy of 0.33° of visual angle (SE = 0.01). Linear perceptrons (LPs) were only nearly half as accurate. For five subjects performing a saccadic eye movement task in the upper right quadrant of the visual field, the mean accuracy provided by the MLPs was 1.07° of visual angle (SE = 0.01) for EOG data, and 0.95° of visual angle (SE = 0.03) for infrared limbus reflection (IRIS®) data. MLPs enabled calibration of 2D saccadic EOG to an accuracy not significantly different to that obtained with the infrared limbus tracking data.

Работа посвящена разработке ИТ-проекта, направленного на реализацию внедрения рекламы в игровой процесс. Для реализации функционала демонстрации рекламы, а также анализа ее эффективности, в работе исследуются методы анализа эффективности цифровой рекламы с применением технологии окулографии, методы отображения рекламы поверх игрового окна, а также методы регистрации движения глаз. Для подтверждения целесообразности внедрения описанного проекта в работе приведены полная модель предприятия, на которое планируется внедрять систему, и на основе этих данных приведен анализ экономической эффективности системы. / The work is devoted to the development of an IT project, aimed at the implementation of the introduction of advertising in the game process. To implement the functionality of advertising demonstration, as well as to analyze its effectiveness, the work investigates the methods of digital advertising effectiveness analysis using oculography technology, methods of advertising display over the game window, as well as methods of eye movement registration. To confirm the feasibility of the described project, the paper presents a complete model of the enterprise, which is planned to implement the system, and based on these data is an analysis of the cost-effectiveness of the system.