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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolutionKey, Kijona Farthing 07 May 2007 (has links)
Porcine reproductive and respiratory syndrome (PRRS) is a viral disease that has devastated the global swine industry since the mid 1980s. Although modified live vaccines (MLVs) are typically used for the prevention of clinical disease, they are not always fully effective. Additionally, acute PRRS outbreaks, characterized by more severe clinical signs, have appeared in herds that were previously vaccinated. In this dissertation, we further analyzed the pathogenesis of PRRSV through genetic characterization, assay development, and quasispecies evaluation using the PRRSV ORF5 gene while also attempting to develop an improved PRRS vaccine.
To explore the possible mechanism for the emergence of acute PRRS, the open reading frame 5 (ORF5) gene encoding the major envelope protein (GP5) of acute PRRSV isolates was characterized. Sequence and phylogenetic analyses revealed that seven of the acute PRRS virus (PRRSV) isolates were related to other N. American PRRSV isolates while one isolate, 98-37120-2, was very closely related to and may have been derived from the MLV, RespPRRS. We also developed a heteroduplex mobility assay (HMA) for quickly identifying PRRSV field isolates with significant nucleotide sequence identities (â d98%) with the MLVs based on the amplification, denaturation, and reannealing of the ORF5 gene of the field isolates with those of MLV reference strains. All of the field isolates that were highly related to RespPRRS (â T2% nucleotide sequence divergence) were identified by the HMA to form homoduplexes with the reference RespPRRS MLV.
We also developed a unique strategy for infecting pigs with PRRSV, known as in vivo transfection, by bypassing the traditional in vitro cell culture step required for in vivo studies. We demonstrated that inoculation of RNA transcripts of a PRRSV infectious cDNA clone directly into the lymph nodes and tonsils of pigs produces active PRRSV infection. Using this method, we also examined the quasispecies populations of PRRSV. Finally, we evaluated the ability of Salmonella choleraesuis to express the PRRSV GP5, and tested its immunogenicity in mice. Based on our data, there was no indication of Salmonella replication in the mice or any evidence of antibody production against S. choleraesuis or PRRSV GP5. / Ph. D.
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The spread of porcine reproductive and respiratory syndrome virus (PRRSV) by genotype and the association between genotype and clinical signs in Ontario, Canada 2004-2007Rosendal, Thomas 30 September 2011 (has links)
An investigation of the distribution of porcine reproduction and respiratory syndrome virus (PRRSV) and factors associated with the presence of PRRSV in Ontario from 2004 – 2007 was conducted. Surveys on the presence of clinical signs associated with PRRS, management practices, animal suppliers, and herd location were administered to the managers of 458 PRRSV positive herds and 61 PRRSV negative herds. Open reading frame (ORF) 5 of the PRRSV genome was sequenced from herds with PRRSV.
PRRSV positive herds were compared to PRRSV negative herds. Management practices associated with being PRRSV positive were: not washing animal- and feed-delivery vehicles, feed-delivery and animal-transport vehicles visiting multiple herds at one time, allowing a truck driver to enter the barn, not requiring visitors to shower prior to farm entry, and not utilizing all-in all-out flow in gilt and finisher barns.
Specific PRRSV restriction fragment length polymorphism (RFLP) genotypes of the ORF5 gene were compared with clinical signs. Herds with RFLP type ‘1-undetermined-4’, ‘1-undetermined-2’ and 1-3-4 were associated with clinical signs in sows and 2-6-2 was associated with finisher mortality compared to herds with vaccine virus. Additionally, genotypes 1-3-4 and 1-8-4 increased in frequency during this study.
The between-herd PRRSV similarity of genome and clinical signs were compared. Abortions and stillbirths were associated with similarity in genetic sequences between herds. This relationship did not extend to those herds where vaccine virus was identified.
Patterns in space and time of herds with different RFLP types of PRRSV were investigated after accounting for ownership. There was weak evidence to suggest local spread the genotype 1-3-4.
The association between genetic similarity and proximity in space, time, ownership, animal, and semen suppliers was tested. Significant correlation was detected for distances up to 30 km. After controlling for ownership, only small associations between breeding stock and semen suppliers and genetic similarity of PRRSV were found.
The spread of PRRSV among herds in Ontario cannot be attributed to any one factor. However, similarity in ownership between herds was a key variable indicating that movement of animals, personnel, and vehicles among herds must be measured in future investigations of PRRSV dynamics. / Ontario Pork and the Canada-Ontario Research and Development (CORD) Program and the OMAFRA/University of Guelph agreement
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Épidémiologie du syndrome reproducteur et respiratoire porcin dans deux régions de densités porcines différentes au QuébecLambert, Marie-Ève 06 1900 (has links)
Le virus du syndrome reproducteur et respiratoire porcin (SRRP) est actuellement l’une des principales menaces pour la santé des troupeaux porcins. Les multiples voies de transmission complexifient l’épidémiologie de l’infection et en font une maladie particulièrement difficile à contrôler. L’objectif général de ce projet de recherche était de déterminer les facteurs associés au statut SRRP des sites de production afin de mieux comprendre l’épidémiologie de cette maladie au sein de deux régions du Québec ayant des densités porcines différentes.
Les stratégies d’introduction des cochettes de remplacement ont d’abord été examinées. Des lacunes importantes ont été identifiées représentant un risque potentiel pour l’introduction du virus ou pour la recirculation d’une souche endémique au sein d’un troupeau reproducteur. Ainsi, appliquée à titre de stratégie de contrôle, l’acclimatation s’est révélée particulièrement problématique. Les principes de base étaient peu respectés, pouvant donc avoir un impact négatif considérable sur la circulation virale au sein du troupeau et potentiellement sur le voisinage immédiat.
La fréquence de plusieurs mesures de biosécurité externe a ensuite été évaluée, permettant d’identifier certains problèmes dont ceux touchant principalement les mesures d’hygiène relatives au protocole d’entrée. Des différences de fréquence entre les régions et les types de production ont également été notées, ce qui peut orienter les interventions de rehaussement. Une classification multivariée a permis de grouper les sites en différents patrons de biosécurité pour constituer par le fait même un index de biosécurité. Cette étape a permis d’évaluer l’association entre certaines caractéristiques de l’élevage et le niveau de biosécurité indiqué par l’index. La distribution géographique des patrons au sein des deux régions, couplée à la détection d’agrégats spatiaux de sites ayant un patron similaire, a également permis de cibler davantage les interventions en fonction de la localisation des sites.
Suite à l’investigation du statut SRRP des sites, une prévalence apparente très élevée a été obtenue pour les deux régions, complexifiant le contrôle de la maladie. L’étude de facteurs de risque dans la région de densité modérée a mis en évidence quatre facteurs associés au statut SRRP positif des sites, soit un inventaire important, la proximité du site porcin immédiat, l’absence de douche ainsi que le libre accès au site par l’équarrisseur. Une action préventive intégrant des mesures de biosécurité spécifiques peut donc être entreprise directement à la ferme au regard des deux derniers facteurs. Le fait d’utiliser un index de biosécurité plutôt que des mesures de biosécurité spécifiques a également été évalué. Les résultats ne supportent pas l’index global dans l’évaluation de l’association entre la biosécurité et le statut SRRP des sites de production.
Finalement, la corrélation entre les distances génétique, euclidienne et temporelle des souches de SRRP, considérant également l’appartenance au même ou à des propriétaires différents, a été évaluée au sein de la région de haute densité. Une corrélation positive entre la distance génétique et euclidienne observée jusqu’à 5 km a souligné l’importance de la propagation régionale impliquant les aérosols, les insectes, d’autres espèces animales ou les objets inanimés. De plus, les souches génétiquement similaires appartenaient davantage à des sites ayant le même propriétaire, ce qui sous-tend des mécanismes de transmission impliquant une source commune d’animaux, d’employés, d’équipement, voire de véhicules. / The economic impact of porcine reproductive and respiratory syndrome virus (PRRSV) on swine industry compelled us to improve our knowledge on the epidemiology of the disease in a perspective of prevention. On-farm and regional management of the disease is complex due to the numerous pathways of transmission of the virus. The main objective of this project was to determine factors associated with PRRS status of production sites in two areas of different swine density in Quebec to improve our knowledge on the epidemiology of PRRS.
Gilt replacement strategies were first investigated and practices potentially at risk for PRRSV introduction or recirculation into the sow herd were identified. Acclimatization, which is reported to be an effective strategy to control PRRSV within a herd, was the most common but was the worst applied strategy in the participating herds. Basic principles were not respected which could enhance PRRSV circulation into the sow herd and potentially in the neighbourhood.
The frequency of different biosecurity practices was also examined and led to the identification of some shortcomings, mainly related to the entrance protocol for people; these should be addressed at the farm level before implementing any PRRS regional control. Differences of frequency were observed between regions and production types, thus using this information could help targeting future intervention of biosecurity enhancement. A biosecurity index was developed by grouping sites in different biosecurity patterns using a multivariable technique of classification. This allowed the identification of associations between biosecurity index and characteristic of sites. The geographical distribution of biosecurity patterns among each region combined with the detection of cluster of sites having similar pattern would help to target intervention based on site location.
PRRS status of sites was determined and a high apparent prevalences of infected sites were obtained in both regions which will complicate PRRS management. In the moderate density area, four variables were associated with PRRS positive status: high inventory, proximity to the closest pig site, absence of shower and free access to the site by rendering trucks. Whereas the first two factors are non modifiable characteristics of site, the other ones can be directly managed on the site by biosecurity. The impact of using a biosecurity index instead of specific biosecurity variables was also evaluated. Results do not support the use of a global index to assess association between biosecurity and PRRS status.
Finally, the correlation among genetic, Euclidean and temporal distances and ownership of PRRSV strains was assessed in the high density area. Positive correlation between genetic distance and ownership suggests either common sources of animals or semen, employees, technical services or vehicles. A positive correlation was also obtained between genetic and Euclidean distances up to 5 km, suggesting the importance of mechanisms involved in area spread such as aerosols, insects, others animal species or fomites.
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Épidémiologie du syndrome reproducteur et respiratoire porcin dans deux régions de densités porcines différentes au QuébecLambert, Marie-Ève 06 1900 (has links)
No description available.
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