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Adaption to environmental stresses is vital for the survival of all organisms living in any environment. Two of the major environmental factors in the deep sea environment are high hydrostatic pressure and high salt concentration. Hydrostatic pressure and osmotic pressure share similarities in their effects on organisms living in the deep sea but this overlap has been little explored. Major studies from Japan and California over the last 40 years have shown the effects of hydrostatic pressure on bacteria from the deep sea (see [1] for a review). These are complemented by work by Yancey et al. [2] showing that specific solutes accumulated in response to osmotic pressure in fish have the ability to enhance resistance to hydrostatic pressure. However, this work has been done in vitro or with larger organisms and not much is known about the overlap of osmotic and hydrostatic pressure in bacteria. In this study I investigated the effects of osmotic and hydrostatic pressure on two model organisms: Photobacterium profundum and Escherichia coli. In order to accomplish this task I developed novel imaging equipment which allows for high resolution imaging of bacteria at pressure. I also developed a new method of growing bacteria in 96-well plates at high pressure, which lead to the identification of a hierarchy of genes essential for the growth of E. coli at pressure. I used the same 96-well plate technique to monitor the growth of P. profundum at differing osmotic and hydrostatic pressures. Furthermore I also attempted to analyse the solutes accumulated by different strains of P. profundum in response to osmotic and hydrostatic pressures.

In 1897 Davenport measured the wet and dry weights of amphibian embryos from the stage of hatching onwards. He observed that there was a continuous increase in the wet weight but that the dry weight remained constant until the embryo began feeding. From this he concluded that "growth is due chiefly to imbibed water". Schaper (1902) noted a similar constancy of dry weight from the early tail bud stages until the time of feeding in embryos of Rana fusca. These early observations have been confirmed by Dempster (1933) who, working with Amblystoma punctatwn, extended his experiments to include the earliest developmental stages. The increase in volume, and hence the growth, of amphibian embryos is therefore due to the uptake of water from the environment. Many embryologists have attempted to correlate this water uptake with the osmotic pressure of the embryos. The early work in this field has been extensively reviewed by Needham (1931).

The study on dehydration of frozen saskatoon berries and the need for dried fruits have been strategically identified in the Canadian Prairies. The motivation for this research was to find a suitable method for dehydration and extend saskatoon berry shelf life for long term preservation. Microwave, convection and microwave-convection combination drying processes were identified to finish-dry saskatoon berries after osmotic dehydration, using sucrose and high fructose corn syrup (HFCS) sugar solutions. Osmotic dehydration removes moisture in small quantities introducing solutes into the fruit that acts as a preservative and also reduces the total drying time. <p>Due to the very short harvesting season of saskatoon berries, an accelerated process such as the microwave combination drying can reduce the moisture to safe storage levels immediately after harvest. Untreated and osmotically dehydrated berries were subjected to convection (control), microwave and microwave-convection combination drying conditions at different product drying temperatures (60, 70 and 80oC) until final moisture content was 25% dry basis. A laboratory-scale microwave combination dryer was developed with integrated temperature and moisture loss data acquisition systems using LabView 6i software. A thin-layer cross flow dryer was used for convection-only drying and for comparison. <p>Drying kinetics of the process were studied and curve fitting with five empirical equations, including the Page equation, was carried out to determine drying constant, R2 and standard error values. The microwave-combination drying method proved to be the best for drying saskatoon berries. Dehydrated product quality analyses were accomplished by measuring the color changes, rehydration ratio and any structural changes, using a scanning electron microscope technique.<p>This research was instrumental in the modification and development of a novel drying system for high-moisture agricultural materials (fruits). Microwave-convection combination drying at 70oC, yielded good results with higher drying rates and better end-product quality.

The study on dehydration of frozen saskatoon berries and the need for dried fruits have been strategically identified in the Canadian Prairies. The motivation for this research was to find a suitable method for dehydration and extend saskatoon berry shelf life for long term preservation. Microwave, convection and microwave-convection combination drying processes were identified to finish-dry saskatoon berries after osmotic dehydration, using sucrose and high fructose corn syrup (HFCS) sugar solutions. Osmotic dehydration removes moisture in small quantities introducing solutes into the fruit that acts as a preservative and also reduces the total drying time. <p>Due to the very short harvesting season of saskatoon berries, an accelerated process such as the microwave combination drying can reduce the moisture to safe storage levels immediately after harvest. Untreated and osmotically dehydrated berries were subjected to convection (control), microwave and microwave-convection combination drying conditions at different product drying temperatures (60, 70 and 80oC) until final moisture content was 25% dry basis. A laboratory-scale microwave combination dryer was developed with integrated temperature and moisture loss data acquisition systems using LabView 6i software. A thin-layer cross flow dryer was used for convection-only drying and for comparison. <p>Drying kinetics of the process were studied and curve fitting with five empirical equations, including the Page equation, was carried out to determine drying constant, R2 and standard error values. The microwave-combination drying method proved to be the best for drying saskatoon berries. Dehydrated product quality analyses were accomplished by measuring the color changes, rehydration ratio and any structural changes, using a scanning electron microscope technique.<p>This research was instrumental in the modification and development of a novel drying system for high-moisture agricultural materials (fruits). Microwave-convection combination drying at 70oC, yielded good results with higher drying rates and better end-product quality.

Microalgae are an important potential feedstock for biodiesel production. Understanding the physiology of lipid biosynthesis in microalgae is pivotal to microalgal aquaculture management. A freshwater green microalga strain, UTEX 2219-4, was isolated from UTEX 2219 which was reported containing two strains. Its ITS sequences are closely related to those in the family of Scenedesmaceae in the GenBank. Nitrogen starvation, salt stress and osmotic stress greatly enhanced lipid biosynthesis in this strain, while combination of nitrogen deficiency and osmotic stress had the most dramatic effect. Chloroplast was condensed and photosynthesis efficiency declined about 50% after 3 days of nitrogen starvation. Chlorophyll degradation followed the same trend but was more severe than the reduction of photosynthesis efficiency. Oil body formation was not observed in the cells kept in the dark under nitrogen starvation, suggesting photosynthesis rather than autophagy is the major player in oil body formation. Under non-saturation levels of light intensities coupled with nitrogen starvation, the oil body formation under 150 £gmol/m2s light intensity was more efficient than that under 75 £gmol/m2s. DCMU blocked photosynthesis as well as oil body formation, supporting that the energy for oil body formation was mostly from photosynthesis rather than autophagy during nitrogen starvation.

Therapeutic angiogenesis is a promising technique to treat ischemia by creating new blood vessels. The aim of this thesis was to develop and characterize biodegradable elastomers for localized delivery of growth factors and to investigate the ability of released growth factors to induce angiogenesis. An osmotic delivery mechanism using photo-cross-linked elastomers based on trimethylene carbonate (TMC) was used to deliver vascular endothelial growth factor (VEGF165) and hepatocyte growth factor (HGF) alone or in combination at two different doses. It was hypothesized that elastomers made of TMC can provide an effective osmotic release using trehalose as a main osmotigen and that the use of TMC would eliminate the microenvironmental pH drop implicated in denaturing acid sensitive growth factors. To obtain an insight into the degrading zone in which growth factors will be released, the in vivo degradation mechanism and tissue response were investigated. The in vivo degradation of D,L-lactide/ε-caprolactone (DLLACL) elastomers that degrade by hydrolysis was investigated for comparison. Cross-link-density played a significant role in the degradation pattern of DLLACL elastomers. TMC and TMCCL elastomers degraded by surface erosion and oxidation played a significant role in their in vivo degradation. To obtain an efficient release, the mechanical properties of TMC elastomers were tailored by copolymerizing TMC with CL and DLLA and/or by controlling the cross-link density. The delivery device was able to provide a sustained release of growth factors for longer than two weeks with no initial burst. Cell based bioactivity assays indicated that released growth factors were highly bioactive over the entire release period. Microenvironmental pH studies using FITC-BSA indicated no significant drop in pH in TMC elastomers that contained small amounts of DLLA. Using 125I-VEGF165, it was found that the osmotic delivery can provide a direct in vivo-in vitro release correlation. Released growth factors were able to induce angiogenesis in rats when tested by subcutaneous implantation. Angiogenesis was dose dependent for both VEGF165 and HGF. Combined release of VEGF and HGF achieved the best results. The formed blood vessels were stable during the active release period, and they were normal looking and connected to the surrounding vasculature. / Thesis (Ph.D, Chemical Engineering) -- Queen's University, 2009-09-03 14:54:28.709