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Biochemical And Genetic Characterization Of Halobacterium Salinarium Strain Isolated From Tuz Lake In Central AnatoliaCakici, Ozgur 01 January 2004 (has links) (PDF)
In this study, a halophilic archaea Halobacterium salinarium TG13 which is isolated from Tuz Lake in Central Anatolia was characterized biochemically and genetically. Halobacterium salinarium DSM3754 and Halobacterium salinarium S9 strains were used as a reference strain through the experiments. In biochemical characterization / total protein profiles of strains was compared by using 1D SDS PAGE. Total protein profile of the isolated strain has shown differences. The SDS-PAGE profile of the purified purple membrane showed only single band by coomassie staining. Molecular weight and pI values of the protein isolated from Halobacterium salinarium TG13 and Halobacterium salinarium S9 were estimated by 2D SDS-PAGE as 22 kD and 5.4, respectively. Photoactivity of purple membrane of the strains was investigated. pH change of the purple membranes were observed upon illumination. This protein might be corresponded to bacteriorhodopsin. In genetical characterization / polymorphism of genomic DNA of strains was scanned with RAPD-PCR. Plasmid DNA profiles of strains was determined to make use of RFLP technique. RAPD-PCR and RFLP analyses have shown that Halobacterium salinarium TG13 is different strain from reference Halobacterium salinarium strains (H.s. S9 and H.s. DSM3754).
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Kvasinky a víno / Yeasts and winePalíková, Petra January 2010 (has links)
This thesis deals with isolation and identification wine yeasts from grapes and must. For analysis was used white wine Sauvignon that was grown and producing after needs ecological agriculture. Remove samples were processed in laboratory and by the help of dilution method were obtained pure culture isolated yeasts. In the following step, by the application of commercial kit UltraCleanTM Microbial DNA Isolation Kit we were able to isolated individual DNA that it was used to the next analysis. Isolated DNA was amplification by PCR method with ITS1 and ITS4 primers. PCR products were detected on agarose gel. Amplification samples were chopped five restriction endonucleases: HaeIII, HinfI, TaqaI, AluI and MseI. Chopped DNA was detected by the same way as PCR products and it was compared with restriction patterns of collection yeasts. In the next step it was compared genetic similarity of isolated yeasts by using BioNumerics software. As a criterion it was used Pearson coefficients and UPGMA clastering analysis. The result is dedrogram of genetics similarity isolated yeasts.
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The commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coliSnelling, Anna M., Macfarlane-Smith, Louissa, Fletcher, Jonathan N., Okeke, Iruka N. 2009 December 1921 (has links)
Yes / Background. The roles of diffusely-adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) in disease are not well understood, in part because of the limitations of diagnostic tests for each of these categories of diarrhoea-causing E. coli. A HEp-2 adherence assay is the Gold Standard for detecting both EAEC and DAEC but DNA probes with limited sensitivity are also employed.
Results. We demonstrate that the daaC probe, conventionally used to detect DAEC, cross-reacts with a subset of strains belonging to the EAEC category. The cross hybridization is due to 84% identity, at the nucleotide level, between the daaC locus and the aggregative adherence fimbriae II cluster gene, aafC, present in some EAEC strains. Because aaf-positive EAEC show a better association with diarrhoea than other EAEC, this specific cross-hybridization may have
contributed to an over-estimation of the association of daaC with disease in some studies. We have developed a discriminatory PCR-RFLP protocol to delineate EAEC strains detected by the daaC probe in molecular epidemiological studies.
Conclusions. A PCR-RFLP protocol described herein can be used to identify aaf-positive EAEC and daaC-positive DAEC and to delineate these two types of diarrhoeagenic E. coli, which both react with the daaC probe. This should help to improve current understanding and future investigations of DAEC and EAEC epidemiology.
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Phylogeny and molecular identification of Cronobacter strains isolated from south African food productsStrydom, Amy 03 1900 (has links)
Thesis (MSc Food Sc)--University of Stellenbosch, 2011. / ENGLISH ABSTRACT: The genus Cronobacter (Enterobacter sakazakii) contains opportunistic pathogens that can
cause a severe form of neonatal meningitis, necrotising enterocolitis and septicaemia.
Cronobacter infections have been reported in all age groups, however, immunocompromised
infants are more susceptible to these infections. Furthermore, Cronobacter
strains have been reported to show differences in sensitivity to antibiotics and virulence.
These differences led to the reclassification of Cronobacter and currently the genus
contains five distinct species, namely Cronobacter sakazakii, Cronobacter malonaticus,
Cronobacter turicensis, Cronobacter dublinensis and Cronobacter muytjensii. As this
reclassification was only accepted recently, there are not many typing methods optimised
for differentiation between the five Cronobacter species. Typing of Cronobacter strains are
important as the species may be diverse regarding their virulence.
Cronobacter strains have been isolated from infant formula milk (IFM), the
environment of an IFM processing facility and fresh produce in South Africa. However,
little is known about the phylogeny and prevalence of these strains. The aim of this study
was to classify 24 South African Cronobacter strains (previously identified as E. sakazakii)
and to evaluate the phylogeny of the isolates based on the 16S ribosomal RNA (rRNA) and
rpoA genes. All 24 South African strains were identified as Cr. sakazakii despite a wide
variety of isolation sources. Other studies have also found that irrespective of the isolation
source, the majority of Cronobacter strains are identified as Cr. sakazakii. The South
African strains were found to be phylogenetically closely related. However, two distinct
clusters separated at a 93 % confidence level were observed in the Cr. sakazakii group
based on the 16S rRNA gene analysis.
Strains of Cr. sakazakii, Cr. dublinensis, Cr. turicensis and Cr. muytjensii were
differentiated from each other with sequence data of the 16S rRNA and rpoA genes, but it
was not possible to differentiate between Cr. sakazakii and Cr. malonaticus. The
phylogram based on the rpoA gene sequences did separate Cr. malonaticus and Cr.
sakazakii strains, however, the clusters were separated with a low bootstrap value of 70 %.
Phylogenetic analysis based on the rpoA and 16S rRNA genes were, therefore, not
sufficient to distinguish between all the Cronobacter species. The sequence data of these
two genes can be used to differentiate between the Cronobacter strains when used in
combination with malonate utilisation analysis.
A PCR-RFLP method was subsequently developed to facilitate the simultaneous
differentiation between all five Cronobacter species. The PCR-RFLP assay was based on
the amplification of the rpoB gene followed by the combined digestion with restriction
endonucleases Csp6I and HinP1I. Unique profiles for each of the five Cronobacter species
were obtained and it was also possible to differentiate between Enterobacteriaceae and
Cronobacter strains. Furthermore, two strains which were identified as Cr. sakazakii with
sequencing based on the 16S rRNA and rpoA genes had PCR-RFLP profiles identical to
that of Cr. malonaticus. Sequencing based on the rpoB gene and additional biochemical
analysis with malonate broth confirmed the identities of these two strains as Cr.
malonaticus. This PCR-RFLP assay is, therefore, an accurate typing method that ensures
rapid differentiation between the five species of Cronobacter. / AFRIKAANSE OPSOMMING: Die Cronobacter genus (Enterobacter sakazakii) bevat opportunistiese patogene wat 'n
ernstige vorm van neonatale meningitis, enterokolitis en septisemie kan veroorsaak.
Cronobacter infeksies is al in alle ouderdomsgroepe aangemeld, maar
immuungekompromitteerde babas is die meeste vatbaar vir hierdie infeksies. Verder toon
Cronobacter spesies verskille in virulensie en sensitiwiteit vir antibiotika. Hierdie verskille
het gelei tot die herklassifikasie van Cronobacter en tans bestaan die genus uit vyf
afsonderlike spesies, naamlik Cronobacter sakazakii, Cronobacter malonaticus,
Cronobacter turicensis, Cronobacter dublinensis en Cronobacter muytjensii. Aangesien
hierdie herklassifikasie slegs onlangs aanvaar is, is daar nie baie metodes wat geskik is vir
onderskeiding tussen die vyf Cronobacter spesies nie. Onderskeiding tussen Cronobacter
spesies is belangrik omdat die spesies verskillend kan wees met betrekking tot hulle
virulensie.
Cronobacter is geisoleer uit baba formule melk (BFM), die omgewing van 'n BFM
fabriek en vars produkte in Suid-Afrika. Daar is egter nie baie bekend oor die filogenie en
voorkoms van hierdie isolate nie. Die doel van hierdie studie was om 24 Suid-Afrikaanse
Cronobacter stamme (voorheen geïdentifiseer as E. sakazakii) te klassifiseer en die
filogenie van die isolate te evalueer gebaseer op die 16S ribosomale RNS (rRNS) en rpoA
gene. Al 24 Suid-Afrikaanse stamme is geïdentifiseer as Cr. sakazakii ten spyte van 'n
wye verskeidenheid isolasie bronne. Ander studies het ook gevind dat, ongeag die isolasie
bron, die meerderheid van Cronobacter stamme as Cr. sakazakii geïdentifiseer word. In
hierdie studie is gevind dat die Suid-Afrikaanse stamme filogeneties nou verwant is. Op
grond van die 16S rRNA geen analise is die Cr. sakazakii stamme egter in twee
afsonderlike groepe gedeel met 'n 93% vertrouens vlak.
Dit was moontlik om stamme van Cr. sakazakii, Cr. dublinensis, Cr. turicensis en Cr.
muytjensii van mekaar te onderskei met die DNS volgorde data van die 16S rRNA en rpoA
gene, maar geen onderskeid tussen Cr. sakazakii en Cr. malonaticus stamme was
moontlik nie. Die filogram gebaseer op die rpoA DNS volgorde data het aparte takke vir Cr.
malonaticus en Cr. sakazakii stamme getoon, maar die twee takke is met ‘n lae vertrouens
waarde van slegs 70 % geskei. Filogenetiese analise gebaseer op die rpoA en 16S rRNA
gene is dus nie voldoende om te onderskei tussen al die Cronobacter spesies nie. Die
DNS volgorde data van hierdie twee gene sou egter gebruik kon word om te onderskei
tussen die Cronobacter spesies wanneer dit gebruik word in kombinasie met
malonaatbenutting-analises.
'n Polimerase ketting reaksie (PKR) beperkings fragment lengte polimorfisme
(BFLP) metode is ontwikkel om die gelyktydige onderskeiding tussen al vyf Cronobacter
spesies te fasiliteer. Die PKR-BFLP metode is gebaseer op die vermeerdering van die
rpoB geen gevolg deur die gesamentlike vertering met die beperkingsensieme, Csp6I en
HinP1I. Unieke profiele vir elk van die vyf Cronobacter spesies is verkry en dit was ook
moontlik om tussen Enterobacteriaceae en Cronobacter spesies te onderskei. Verder het
twee stamme wat as Cr. sakazakii geïdentifiseer is met DNS volgordebepaling van die 16S
rRNA en rpoA gene, PKR-BFLP profiele identies aan dié van Cr. malonaticus getoon.
DNS volgordebepaling van die rpoB geen en ‘n addisionele biochemiese toets met
malonaat sop het die identiteit van hierdie twee stamme as Cr. malonaticus bevestig.
Hierdie PKR-BFLP is dus 'n akkurate metode wat vinnige onderskeid tussen die vyf
spesies van Cronobacter kan verseker.
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Analýza výskytu vybrané dědičné choroby očí u psůKUBIČKOVÁ, Miroslava January 2017 (has links)
Progressive rod-cone degeneration (PRCD) is the late form of progressive retinal atrophy (PRA). It is an autosomal recessive hereditary retinal defect. This disease in dogs is consistent with one form of retinitis pigmentosa (RP) in humans. Phenotypic manifestations are identical and it is known to be an identical causal mutation. A study of this defect in dogs could also explain a lot in human medicine. The gene for PRCD was mapped in the region of centromer of the canine chromosome 9 (CFA9). In this thesis, genotyping of 120 dogs of different breeds and age was performed. Most represented a breed of English Cocker Spaniel which is predisposed to the disease. Analysis PRA-PRCD was performed by molecular genetic methods PCR-RFLP and the horizontal agarose electrophoresis. Genotypes were determined on the basis of different fragment lengths. The normal allele was 396 bp in length and the mutated allele had a length of 116 bp. Presence of mutated allele was only detected in 25 heterozygotes carriers which were usually breeds with this predisposition. Frequency of the mutated allele was 10.4 %. In the selected population 20.8 % of heterozygotes were represented. The results of the study show approximately one fifth of the tested dogs are heterozygous carriers. Findings of other studies confirm there are generally more heterozygotes than homozygotes in which the disease is manifested during life. However, if this fact is not clearly taken in consideration, the number of sick dogs can rapidly increase during short period of time. In the future, it would be appropriate to adopt measures which would definitely eliminate the occurrence of the mutated allele. These measures could include genetic tests that reliably reveal hidden carriers (heterozygotes) in predisposing breeds. Heterozygotes may increase the representation of this allele in the population. This leads to an increase in the number of diseased animals.
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Untersuchungen zum Vorkommen von Toxoplasma gondii in Wild in BrandenburgStollberg, Kaya Christina 16 June 2023 (has links)
Die Toxoplasmose, nach ihrem Verursacher, dem Parasiten Toxoplasma gondii (T. gondii) benannt, ist eine weltweit häufig auftretende Zoonose. Einen wichtigen Infektionsweg stellt der Verzehr von nicht ausreichend erhitztem oder rohem Fleisch, welches Gewebezysten enthält, dar.
In Deutschland hat der Konsum von Wildbret in den letzten 10 Jahren zugenommen. Schwarzwild (Sus scrofa), Rehwild (Capreolus capreolus), Damwild (Dama dama) und Rotwild (Cervus elaphus) sind das am häufigsten gejagte Schalenwild in Deutschland. Dennoch gibt es nur wenige Informationen über das Vorkommen
von T. gondii in Wildtieren in Deutschland, so dass die Bedeutung von Wild als Quelle für eine Infektion des Menschen mit T. gondii weitgehend unbekannt ist.
Ziel dieser Studie war es, die Datenlage zum Vorkommen von T. gondii in Schwarzwild, Rehwild, Damwild und Rotwild in Deutschland zu verbessern und eine fundierte Bewertung des von dem Verzehr von Wildtieren ausgehenden potenziellen Risikos zu ermöglichen.
Neben dem indirekten serologischen Nachweis soll der Nachweis mittels direkter Nachweisverfahren einen Einblick in die tatsächliche Anwesenheit von T. gondii im Muskelgewebe und einen potenziellen Zusammenhang von Seropositivität und der Anwesenheit von T. gondii im Gewebe geben.
In den Jahren 2017-2020 wurden in Brandenburg 306 Stück Schwarzwild, 184 Stück Rehwild, 80 Stück Damwild und 65 Stück Rotwild beprobt. Den 635 Wildtieren wurden Blutproben und Proben von Herz- und Vorderlaufmuskulatur entnommen.
Das aus den Blutproben gewonnene Serum wurde mittels eines kommerziell erhältlichen ELISA untersucht.
Zum direkten Nachweis von T. gondii wurde zur Analyse der aus den Muskelproben gewonnenen DNA eine real-time PCR (qPCR) eingesetzt, die auf das 529-bp-repetitive Element abzielt.
Die DNA wurde auf drei unterschiedliche Arten gewonnen: direkt aus 5 g Muskelgewebe extrahiert, aus einem Pellet nach saurem Pepsinverdau von 50 g Muskelgewebe extrahiert, und die Ziel-DNA durch Magnetic Capture aus weiteren 50 g Muskelgewebe angereichert.
Die Übereinstimmung der Ergebnisse des molekularen Nachweises und ihre Übereinstimmung mit den ELISA-Ergebnissen wurde ermittelt. Der molekulare Nachweis wurde bei 23 Proben von einem Maus-Bioassay begleitet. Zusätzlich wurde eine PCR-RFLP zur Genotypisierung bei qPCR-positiven Proben durchgeführt. Fisher’s exact Test, Cohen’s kappa (κ) und Odds Ratio wurden für die
statistische Analyse genutzt.
T. gondii-spezifische Antikörper wurden in 20,3 % der Schwarzwildproben, 10,9 % der Rehwildproben und 6,2 % der Rotwildproben nachgewiesen. Bei allen untersuchten Wildarten wurde ein Anstieg der Seroprävalenz mit zunehmendem Alter festgestellt, welcher bei Schwarzwild und Rehwild statistisch signifikant war (p = 0,004 und < 0,001).
Bei der Untersuchung von Herzmuskulatur wurde T. gondii-DNA mit mindestens einer direkten Nachweismethode in 11,8 % der Schwarzwildproben, 5,5 % der Rehwildproben, 2 % der Damwildproben und 1,9 % der Rotwildproben nachgewiesen. Der höchste Anteil an Tieren, die positiv auf T. gondii-DNA getestet wurden, wurde durch die qPCR-Analyse von DNA aus 50 g Herzmuskulatur nach Magnetic Capture nachgewiesen (10 %). Die Ergebnisse der Methoden zeigten
insgesamt eine mäßige Übereinstimmung (κ = 0,47-0,59). Die höchste Übereinstimmung zeigten die Ergebnisse von DNA aus 50 g Herzmuskulatur nach Pepsin-Verdau und DNA aus 50 g Herzmuskulatur nach Magnetic Capture (κ = 0,59). Insgesamt ergab die Untersuchung von 50 g Herzmuskulatur einen signifikant höheren Anteil an positiven qPCR-Ergebnissen als die Analyse von
5 g Herzmuskulatur (p = 0,048).
Die qPCR-Ergebnisse von Herz- und Vorderlaufmuskelgewebe zeigten beim Schwarzwild eine beachtliche und bei der gemeinsamen Betrachtung aller untersuchten Wildarten eine mäßige Übereinstimmung (κ = 0,62 bzw. 0,46).
Ein statistisch signifikanter Zusammenhang zwischen Seropositivität und direktem Nachweis war bei Schwarzwild und Rehwild erkennbar (p < 0,001).
In allen T. gondii-DNA-positiven Proben, bei denen zusätzlich ein Bioassay durchgeführt wurde, konnte Infektiosität bestätigt werden (4/4).
Sowohl in den T. gondii-DNA-positiven Schwarzwildproben als auch in den positiven Rehwildproben waren die spezifischen Allele von T. gondii-Typ II am weitesten verbreitet.
Die durch diese Arbeit generierten Daten zeigen, dass T. gondii in Schwarzwild, Rehwild, Damwild und Rotwild in Brandenburg vorkommt und Wild eine relevante Quelle für T. gondii-Infektionen beim Menschen darstellen könnte.
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A model system using insects to vector Fusarium tumidum for biological control of gorse (Ulex europaeus)Yamoah, Emmanuel January 2007 (has links)
The overall objective of this study was to test the hypothesis that insects can vector F. tumidum conidia to infect gorse plants with the aim of developing an alternative approach to mycoherbicide delivery to control weeds. Four potential insect species (Apion ulicis, Cydia ulicetana, Epiphyas postvittana and Sericothrips staphylinus) were assessed for their ability to vector F. tumidum conidia. To achieve this, the external microflora (bacteria and fungi) and the size and location of fungal spores on the cuticle of these insect species were determined. In addition, the ability of the insects to pick up and deposit F. tumidum conidia on agar was studied. Based on the results from these experiments, E. postvittana was selected for more detailed experiments to determine transmission of F. tumidum to infect potted gorse plants. The factors promoting pathogenicity of F. tumidum against gorse and the pathogen loading required to infect and kill the weed were also determined. The external microflora of the four insect species were recovered by washing and plating techniques and identified by morphology and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and sequencing of internally transcribed spacer (ITS) and 16S rDNA. A culture-independent technique (direct PCR) was also used to assess fungal diversity by direct amplification of ITS sequences from the washings of the insects. All insect species carried Alternaria, Cladosporium, Nectria, Penicillium, Phoma, Pseudozyma spp. and entomopathogens. Ninety four per cent of the 178 cloned amplicons had ITS sequences similarity to Nectria mauritiicola. E. postvittana carried the largest fungal spores (mean surface area of 125.9 µm²) and the most fungal CFU/insect. About 70% of the fungi isolated from the insects were also present on the host plant (gorse) and the understorey grass. The mean size of fungal spores recovered from the insect species correlated strongly with their body length (R² = 85%). Methylobacterium aquaticum and Pseudomonas lutea were common on all four insect species. Pseudomonas fluorescens was the most abundant bacterial species. In the pathogenicity trials, the effectiveness of F. tumidum in reducing root and shoot biomass of 16 and 8 wk old gorse plants was significantly increased with wounding of the plants. Older plants (32 wk old) which were wounded and inoculated were significantly shorter, more infected and developed more tip dieback (80%) than plants which were not wounded (32%). This indicates that damage caused by phytophagous insect species present on gorse through feeding and oviposition may enhance infection by F. tumidum. Wounding may release nutrients (e.g. Mg and Zn) essential for conidia germination and germ tube elongation and also provide easier access for germ tube penetration. Conidial germination and germ tube length were increased by 50 and 877%, respectively when incubated in 0.2% of gorse extract solution for 24 h compared with incubation in water. Inoculum suspensions amended with 0.2% of gorse extract caused more infection and significantly reduced biomass production of 24 wk old gorse plants than suspensions without gorse extract. A minimum number of about 900 viable conidia/infection site of F. tumidum were required to infect gorse leaves. However, incorporation of amendments (which can injure the leaf cuticle) or provision of nutrients (i.e. gorse extract or glucose) in the formulation might decrease the number of conidia required for lesion formation. Scanning electron micrographs showed that germ tube penetration of gorse tissue was limited to open stomata which partly explain the large number of conidia required for infection. The flowers and leaves were more susceptible to F. tumidum infection than the spines, stems and pods. An experiment to determine the number of infection sites required to cause plant mortality showed that the entire plant needs to be inoculated in order for the pathogen to kill 10 wk old plants as F. tumidum is a non systemic pathogen. The number of infection sites correlated strongly with disease severity (R² = 99.3%). At least 50% of the plant was required to be inoculated to cause a significant reduction in shoot dry weight. F. tumidum, applied as soil inoculant using inoculated wheat grains in three separate experiments, significantly suppressed gorse seedling emergence and biomass production. In experiments to determine the loading capacity of the insect species, E. postvittana, the largest insect species studied, carried significantly more (68) and deposited significantly more (29) F. tumidum conidia than the other species. Each E. postvittana, loaded with 5,000 conidia of F. tumidum, transmitted approximately 310 conidia onto gorse plants but this did not cause any infection or affect plant growth as determined by shoot fresh weight and shoot height. E. postvittana on its own did not cause any significant damage to gorse and did not enhance F. tumidum infection. It also failed to spread the pathogen from infected plants to the healthy ones. There was no evidence of synergism between the two agents and damage caused by the combination of both E. postvittana and F. tumidum was equivalent to that caused by F. tumidum alone. This study has shown that E. postvittana has the greatest capacity to vector F. tumidum since it naturally carried the largest and the most fungal spores (429 CFU/insect). Moreover, it naturally carried Fusarium spp. such as F. lateritium, F. tricinctum and Gibberella pulicaris (anamorph Fusarium sambucinum) and was capable of carrying and depositing most F. tumidum conidia on agar. Coupled with the availability of pheromone for attracting the male insects, E. postvittana may be a suitable insect vector for delivering F. tumidum conidia on gorse using this novel biocontrol strategy. Although it is a polyphagous insect, and may visit non-target plants, F. tumidum is a very specific pathogen of gorse, broom and a few closely related plant species. Hence, using this insect species to vector F. tumidum in a biological control programme, should not pose a significant threat to plants of economic importance. However, successful control of gorse using this "lure-load-infect" concept would depend, to a large extent on the virulence of the pathogen as insects, due to the large size of F. tumidum macroconidia, can carry only a small number of it.
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Vliv vybraných kandidátních lokusů na ukazatele jakosti masa u skotu / Effect of selected candidate loci on meat quality indicators by cattleSTEBLOVÁ, Halina January 2014 (has links)
This study was aimed on analysis of polymorphism at position 2141 in exon 5 of the growth hormone gene (GH) and polymorphism at position 257 in exon 10 of the growth hormone receptor gene (GHR) and to determine the influence of these polymorphisms on meat tenderness. To analysis has been used 333 meat samples of Czech Pied bulls. Genotypization of GH and GHR loci was performed by PCR-RFLP. For detection of single nucleotide polymorphism in both genes was used restriction endonuclease AluI. To determine meat tenderness was used the method of measuring shear force by Warner and Bratzler. The shear force values of raw meat samples were measured at day 14 post mortem. Then was statistically evaluated the association between genotypes and shear force. In the study population occurred for GH locus 166 individuals with genotype LL, 161 heterozygotes LV and 6 homozygotes VV. The relative genotype frequencies were thus 0.499 (LL), 0.483 (LV) and 0.018 (VV). The frequency of L allele was 0,74 and of V allele was 0,26. For GHR locus was found in the study population 178 homozygotes AA, 105 heterozygotes AG and 50 homozygotes GG. The relative frequencies of genotypes were 0.535 (AA), 0.315 (AG) and 0.15 (GG). The frequency of A allele was 0,692 and of G allele was 0,308. Using statistical analysis revealed a significant effect of genotype GH gene on shear force, tenderness resp. (P<0,05). For GHR locus showed no effect of genotype on shear force (P>0,05).
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Kongenitální choroby skotu / Congenital disorders by cattleKOSOBUDOVÁ, Hana January 2012 (has links)
In the framework of this thesis was performed genotyping of 46 specimens of the breed Czech red cattle from the University farm in Czech Budejovice, which monitored the incidence of autosomal recessive genetic disorders, specifically bovine citrullinemia (BC) in exon 5 and deficiency of blood coagulation factor XI (FXI) in exon 9 and 12. Genotyping for BC was done using PCR/RFLP methods and for the disorder FXI in both exons genotypes were determined on the basis of different length of fragments using PCR technology and horizontal agarose electrophoresis. The presence of mutant allele was detected only in the locus for BC and that is in 7 heterozygous carriers, who produced three bands with a length of 185 bp fragments, 103 bp and 82 bp. The frequency of mutant allele and the frequency of heterozygous carriers to 7.6% and 15.2%. Results of the study show that the presence of mutant allele for BC in our tested panel of animals is relatively high. In the future it will be necessary to adopt measures that will lead to the elimination of this allele. Otherwise, its further dissemination would have a negative impact on the health of the population and there might occur complications in the regeneration of Czech red cattle, which is one of our farm animal genetic resources. The literature review deals with the problems of congenital disorders and discusses the importance of health heredity and understanding of the genomic information of cattle.
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Genetic aspects of hearing loss in the Limpopo Province of South Africa.Kabahuma, Rosemary I. 27 August 2010 (has links)
The aetiological diagnosis of recessive non-syndromic hearing loss poses a challenge owing
to marked heterogeneity and the lack of identifying clinical features. The finding that up to
50% of recessive non-syndromal genetic hearing loss among Caucasians was due to
mutations in GJB2, the gene encoding Connexin 26 (Cx26) was a breakthrough, whose value
as a diagnostic tool has been limited by the significant variation in the prevalence of deafness
genes and loci among population groups. The significant association of the GJB6-D13S1830
deletion among individuals with one mutant GJB2 allele highlighted the need to explore
population specific genetic mutations for NSHL. Although data from Sub-Saharan Africa is
limited, reported studies found a high prevalence of R143W GJB2 mutation among
Ghanaian, the 35delG mutation in 5 out of 139 Sudanese and a low prevalence of GJB2
variations among 385 Kenyan deaf children. The mutation spectrum of Waardenburg
Syndrome (WS) in Africans has not been documented.
During a visit to a School for the Deaf in the Limpopo Province of South Africa in 1997, it
was noted that a high number of students came from Nzhelele sub-district. All had childhood
onset hearing loss with no associated anomalies or disorders. The question arose as to
whether there was a high-risk area for deafness in the Limpopo Province and what the
aetiology of this hearing loss was.The main aim of this study was to investigate the role of
GJB2, the GJB6-D13S1830 deletion, and the four common mitochondrial mutations,
A1555G, A3243G, A7511C and A7445G, in the African hearing-impaired population of
Limpopo province in South Africa, and to identify the mutation spectrum of the deafness
genes found. The type and degree of hearing loss in this hearing impaired population would
also be assessed. Secondly, this study sought to identify the mutations in a sibling pair with
2
clinical WS and to use the findings in a future study to establish the mutation spectrum of WS
in the African population of the Limpopo province and of South Africa in general.
The study was designed as a two phase study, in which phase 1 was used for hypothesis
formulation and phase 2 was for hypothesis testing. While phase 1 was a descriptive
retrospective case study, phase 2 was a combination of sample survey and prospective
descriptive case study. In phase 1, demographic data of 361 students in two schools of the
deaf in the Limpopo province was analyzed for evidence of areas of high risk populations for
deafness in the province. In phase 2, a group of 182 individuals with genetic non-syndromic
hearing loss (NSHL) and two siblings with clinical WS from two schools for the Deaf in the
Limpopo Province of South Africa were investigated. A thorough clinical examination,
audiological evaluation and urinalysis were done. Mutational screening was carried out in all
184 subjects using genomic DNA using single-strand conformation polymorphism (SSCP),
multiplex polymerase chain reaction (PCR), and direct sequencing for GJB2, and Restriction
Fragment-Length Polymorphism (PCR–RFLP) analysis for GJB6, and SSCP, hetero-duplex
analysis, and direct sequencing of the first 8 exons of PAX3 and all of MITF for Waarenburg
syndrome. Data analysis was by geographical mapping, frequency tables, tests of association
with calculation of odds ratios, and binary logistic regression analysis using STATA and GIS
mapping systems.
The results indicate that there seem to be areas of genuine populations at risk for hearing loss
in the Limpopo province of South Africa, namely Mutale and parts of Makhado and
Thulamela municipalities. In Thulamela (NP343) wards 11-15, 26-30 and 31-35, and in
Mutale (NP 344) wards 6-10, together accounted for 67 (18%) of participants in phase 1, and
33 (18%) of the participants in phase 2 of the study. Mutale municipality in the Vhembe
3
district gave with a projected prevalence of at least 13.14 deaf children per 100,000 African
population attending the local school for the deaf.
The observed hearing loss is a genetic, non-syndromic form, which is mainly severe and
severe to profound, although without any clear defining configuration or shape. It is a stable,
non-progressive and prelingual form of hearing loss, implying that this may be a recessive
form of deafness. No identifiable environmental confounding factors or associations were
identified. The deafness is not linked the common known auditory gene mutations in GJB2,
the GJB6-D13S1830 deletion, or the common mitochondrial mutations A1555G, A3243G,
A7511C and A7445G. Severe and profound levels of hearing loss were found in 22.8% and
75% of the cohort respectively, with the majority exhibiting flat (70.1%) or sloping (23.4%)
audiograms that were commonly symmetrical (81.5%). However, as indicated, there was no
clear pattern in the audiological findings overall.
None of the 184 hearing impaired individuals exhibited any of the reported disease causing
mutations of GJB2, including 35delG. There was, however, a high prevalence of two
variants, the C>T variant at position g.3318-15 and the C>T variant at position g.3318-34,
occurring in 21.4% and 46.2% of the deaf cohort respectively. The same variants were found
to occur in 35% and 42.6% of a normal hearing control group (n = 63) respectively,
indicating that these variations are polymorphisms. In three subjects (1.63% of the cohort), a
T>A homozygous variation at position g.3318-6 was detected. Its significance in the
causation of NSSNHL is yet to be determined. The GJB6-D13S1830 deletion was not
detected in any of the participants. None of the four mitochondrial mutations screened for
were found.
4
These results indicate that GJB2 is not a significant deafness gene in the African population
of the Limpopo Province of South Africa and that significant genes for non-syndromic
recessive hearing loss in this population are yet to be found. The geographical clustering of
deafness found in this study, combined with the lack of identifiable common associated
clinical features among the subjects of this study (excluding the WS sibling pair), suggests
that these subjects have a genetic recessive non-syndromal type of hearing loss. In the context
of historical and cultural evidence of consanguinity in this population, a founder effect cannot
be ruled out.
A rare mutation, R223X, previously identified only once out of 470 WS patients, was
identified in the PAX3 gene among the WS sibling pair. A novel silent change GGG>GGT at
amino acid 293, was also identified. These identical findings document, for the first time, a
molecular defect in WS in an African sibling pair, and confirm WS Type I in this family,
which could be found in other WS type I South Africans in the Limpopo Province of South
Africa.
The current study demonstrated that parents of genetically hearing impaired children in these
areas are able to detect hearing loss at an early age, with over 60% suspecting their children’s
hearing loss below 6 months of age. A child-centered management model encompassing all
the areas relevant to childhood deafness/hearing impairment, which takes into consideration
the prevailing logistical and financial constraints of the available healthcare system, is
proposed. The implementation of this model requires a paradigm shift from the current
fragmented model of service delivery to a cohesive patient-centered approach, based on
concrete data from appropriate community based research, in which all the relevant parties
communicate and share resources.
5
It would achieve the goals of early detection and intervention, as well as inclusive education
for all. The relevant health and education policies are already in place and the posts funded.
Equitable implementation of these policies would require appropriate community based
research, as well as improved communication and consultation between the various
stakeholders to ensure an efficient and affordable quality healthcare service for all hearing
impaired South Africans.
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