Monterey pine (Pinus radiata), a native tree to California and two Mexican islands, is important both ecologically and economically. Outside native stands, Monterey pines are grown for landscaping in California and on plantations around the world. Pitch canker, a disease caused by the fungus Gibberella circinata Nirenberg & O’Donnell (Fusarium circinatum Nirenberg and O'Donnell) is threatening the survival of Monterey pines. The disease currently affects Monterey pines in many parts of the world including the native stands. No effective chemical or biological control is available but some Monterey pines show resistance to the disease. The purpose of this project was to develop a working protocol for producing genetic clones of the resistant pines through micropropagation. These genetic clones will be used for outplanting in places outside the native stands for ornamental and plantation purposes. This project analyzes the results of ten trials with varied parameters and bases the final protocol on the parameters used in the trial that induces the growth of new shoots. The final protocol developed in this project describes, step-by-step, the media preparation for the initiation, plant material collection, surface sterilization of plant material, plating in media and initiation of shoots on explants. The protocol calls for collecting shoot tips with hardened buds that have not yet elongated, then washing the shoot tips in sterile water with Tween 20 for 15 minutes. The shoots tips are then surface sterilized in a 50% bleach solution for 20 minutes. The explants are broken into disks (to minimize damage to the cells) by inserting the tip of a scalpel and tilting it slightly. The initiation media shown to induce growth consists of ½ strength LePoivre basal salt mixture, 5mg/L benzylaminopurine, 3% sucrose and 0.8% agar and is adjusted to a pH of 5.7, then autoclaved for 20 minutes. The explants are inserted into solidified media and incubated in a growth chamber programmed for 16 hours of light and 8 hours of dark with temperatures of 27ºC and 22ºC and light irradiance of 80µEm-2s-1. After 1 month the protocol calls for transferring the growing shoots to elongation media with full LP basal salts and transferring every month. When the number of desired shoots has been reached the forthcoming protocol for rooting can be followed.

The relationship between clone and cone color in western white pine, Pinus monticola Douglas, to attack by the mountain pine cone beetle, Conophthorus monticolae Hopkins, was studied in the Sandpoint Seed Orchard, Idaho. A positive relationship was shown to exist during a 5 year field evaluation. Cone beetles were found to prefer dark colored cones and to attack certain clones at a higher rate than others. Laboratory dissections did not indicate that cone color affected oviposition, brood development or brood mortality. Olfactometer experiments demonstrated that olfactory stimuli are involved in the cone beetle attack sequence. Visual cues relating to cone color may be involved in the initial long range host orientation of attacking beetles.

Whitebark pine (Pinus albicaulis Engelmann) is a treeline species in the central Rocky Mountains. Its occupation of high elevations previously protected whitebark pine from long-term mountain pine beetle outbreaks. The mountain pine beetle, however, is currently reaching outbreaks of record magnitude in high-elevation whitebark pine. We used a factorial laboratory experiment to compare mountain pine beetle (Dendroctonus ponderosae Hopkins) life history characteristics between a typical host, lodgepole pine (Pinus contorta Engelmann), and whitebark pine. We tested the effects of natal host and brood host on beetle fecundity, offspring size, and brood sex-ratio. We reared mountain pine beetles from whitebark pine and from lodgepole pine, and infested half of them into their natal host and half into the other host. Fecundity was greater overall in lodgepole pine brood hosts. Among lodgepole brood hosts, beetles from whitebark pine had greater fecundity. Fecundity was also significantly related to phloem thickness, which was greater in lodgepole pine. Offspring were larger from whitebark brood hosts than from lodgepole, regardless of their parents’ natal host. Finally, sex-ratio was closer to 1:1 in lodgepole than in whitebark brood hosts. We conclude that host species affects life history of mountain pine beetle with consequences for individual beetle fitness.

Over the last two decades, mountain pine beetle (Dendroctonus ponderosae) populations reached epidemic levels across much of western North America, including high elevations where cool temperatures previously limited beetle persistence. Many high-elevation pine species are susceptible hosts and experienced high levels of mortality in recent outbreaks, but co-occurring Great Basin bristlecone pine (Pinus longaeva), the longest-living non-clonal organism, were not attacked. I assessed Great Basin bristlecone pine resistance to mountain pine beetle by evaluating mountain pine beetle host selection behavior and reproductive success in this species. To evaluate mountain pine beetle host selection preference for Great Basin bristlecone pine, I used no-choice 48-hour attack box experiments that confined pioneering female beetles onto pairs of living Great Basin bristlecone and limber pine (P. flexilis), a susceptible host tree species. To investigate the effect of induced tree defenses on host selection behavior, I repeated the tests on paired sections of Great Basin bristlecone and limber pines that had been recently cut, thereby removing their capacity for induced defensive reactions to an attack. Mountain pine beetles avoided Great Basin bristlecone pine relative to limber pine, suggesting that Great Basin bristlecone pine has a high level of resistance to mountain pine beetle due at least in part to stimuli that repel pioneering females from initiating attacks, even when induced defenses are compromised. To investigate mountain pine beetle reproductive success in Great Basin bristlecone pine, I compared the mating success, fecundity, and brood production of mountain pine beetle parents placed in cut Great Basin bristlecone pine bolts with that of mountain pine beetles placed in cut bolts of limber pine and lodgepole pine (P. contorta), two susceptible species. Initial reproductive development was similar in all three tree species, but nearly all brood in Great Basin bristlecone pine died before emerging. The extensive offspring mortality observed in Great Basin bristlecone pine may be a key evolutionary driver behind mountain pine beetle aversion to the species. These findings suggest that Great Basin bristlecone pine is a highly resistant species with low vulnerability to climate-driven increases in mountain pine beetle outbreaks at high elevations.

From the end of Reconstruction to the beginning of World War II, no other ecological change affected as great a part of the southern landscape as the loss of the longleaf pine from the southeastern coastal plain. This dissertation examines the causes and consequences of the species’ disappearance. In the span of just decades, lumber operations and naval stores producers descended upon longleaf pine woodlands with a voracious appetite that greatly contributed to the demise of the pine. However, as this dissertation argues, exploitation by the hands of the timber and turpentine industries was not the only agent that transformed the ecoregion. The development of American conservation and forestry, ironically, played a significant role in this process and contributed to the rise of a new southern forest, now stocked with another pine – the loblolly. By looking at the biologists, chemists, and foresters who studied the longleaf for the United States Department of Agriculture and various state agencies from the late nineteenth to the early twentieth century, this dissertation traces how forest sciences in the Gilded Age and Progressive Era shaped the modern ecology of the South. These sciences, too, were entangled in the social and political realities of Jim Crow. Researchers had to ensure that their measures conformed to a segregated society if conservation was to take root in southern woodlands. The conservation practices that federal and state agents put into place as forestry developed into an important and profitable science had profound impacts on not only the land but also those at the bottom of a racial caste system.

Although it is common to model modulus of elasticity (MOE) and modulus of rupture (MOR) of graded lumber as normal, lognormal, or Weibull distributions, recent theories and empirical practices have cast doubt on these models. Mathematical proofs have been used to shown how the MOR distributions of graded lumber can be derived from the MOR distributions of mill-run populations. The MOR distribution of a graded lumber subpopulation is "pseudo- truncated" and does not exhibit the same theoretical form as the mill-run population from which it was drawn. Therefore, it is essential to explore the properties of mill-run lumber populations and properly characterize their MOE and MOR distributions. To investigate this topic, this dissertation has three objectives: 1) to determine if the within-mill means and standard deviations of MOE and MOR in mill-run southern pine (Pinus spp.) lumber differ over time, 2) to determine the correlations among hand-held grain angle meter readings, MOE, and MOR in mill- run southern pine lumber, and 3) to model statistical distributions of MOE and MOR in mill-run red pine (Pinus resinosa) and spruce (Picea spp.) lumber. This research features four main sections: 1) an introduction summarizing the conclusions of each chapter, 2) a chapter investigating if there are statistically significant differences between the means and variances of MOE and MOR in mill-run southern pine lumber populations at the same mill over time, 3) a chapter evaluating the bivariate correlations among handheld grain angle meter readings, MOR, and three measures of MOE in mill-run southern pine lumber, and 4) a chapter modeling the distributions of MOE and MOR in mill-run red pine and spruce lumber populations and comparing those to previous work on mill-run southern pine lumber populations.

This study was a part of the international collaborative IMPACT project, which aims to address the potential threat that the pitch canker disease poses to the use of Pinus radiata D. Don in plantations in New Zealand, Australia, and Chile. A field trial of 264 seedstocks was planted adjacent to a native stand of pitch canker infected P. radiata on the central coast of California, and disease symptom development was recorded over a period of 3 years. The results did not correlate with a greenhouse study of the same seedstocks inoculated with Fusarium circinatum Nirenberg & O'Donnell, the causal agent of pitch canker. Three main types of symptoms were identified (branch flagging, pitchy buds, and chlorotic tips), and preliminary isolation analyses suggest that the disease observed is actually caused by Diplodia pinea (Desm.) Kickx. Survival analysis showed that the effect of tree genetic origin was significant to its time to disease, and that spatial location in the plantation was also significant. Average nearest neighbor analysis showed disease distribution to be significantly clustered, which also suggests that the disease is not pitch canker, but diplodia blight. This experiment illustrates the difficulty in performing naturally infected field trials when another similar-looking fungal disease is also present. It also provides data on seedstock resistance to diplodia blight, another fungal disease important to P. radiata forest managers.

Little information is available to nonindustrial-private forest (NIPF) owners regarding economic tradeoffs between managing pine plantations for timber production or wildlife habitat. Loblolly (Pinus taeda) and longleaf (Pinus palustris) pine plantations were modeled to quantify economic tradeoffs for competing management scenarios utilizing densities aimed at wildlife habitat or timber production in Mississippi. Models contained a range of site indices, planting densities, and rotation lengths for timber maximization and white-tailed deer (Odocoileus virginianus) or northern bobwhite (Colinus virginianus) habitat production. All management scenarios in loblolly plantations produced positive Land Expectation Values (LEVs), while one fifth of the management scenarios in longleaf plantations produced positive LEVs. Comparison to the regional hunting lease rate the compensatory lease rates from the study could be realized, making wildlife management as valuable as timber management. The results of this study will help landowners be more informed about economic tradeoffs when making management decisions on their property.

The objective of the research is to reduce the volatile organic compounds (VOCs) emitted during kiln drying of southern yellow pine (SYP) lumber. Three treatment methods were explored to determine their effect on VOC emissions. The three methods were configured at the air exhaust of a pilot-scale dry kiln. 1) A steel reactor containing copper tubing heated to 100°, 200°, and 240° Celsius was evaluated. Temperature did not statistically affect VOC emissions (p-value = 0.1674). The average reduction of VOCs at 240° C was seven percent. 2) The use of hydrogen peroxide and ultra-violet (UV) light did not reduce VOCs emitted during kiln drying. 3) The use of Fenton?s reagent, with hydrogen peroxide and ferrous sulfate, also did not reduce VOCs emitted during kiln drying. 4) It is recommended that future studies with copper heated to temperatures above 240° C be performed.

The purpose of this research was to increase understanding of ecology and control of cogongrass (Imperata cylindrica (L.) Beauv.) to assist with management of this invasive grass. To address different aspects of cogongrass management, I examined factors that affect spread of cogongrass, effects of cogongrass on native plant communities, and use of three native species to suppress cogongrass. Relative susceptibility of pine-bluestem and pine-shrub vegetation associations to vegetative encroachment and seed dispersal of cogongrass were evaluated. Vegetative encroachment into burned and unburned areas of these two vegetation associations also was measured. Effects of infestation size on vegetative growth of cogongrass and relationships between military activity and establishment and growth of cogongrass on military firing points were investigated. I also compared frequency of cogongrass infestation and vegetative growth rates for unpaved roads with different levels of traffic and maintenance. Native plant richness, diversity, and abundance were compared between cogongrass infestations and in uninfested adjacent areas for longleaf pine-bluestem and ruderal, cleared areas. Morella cerifera (L.) Small, Chamaecrista fasciculata (Michx.) Greene, and Panicum virgatum L. were tested for their ability to suppress cogongrass re-growth after treatment with a herbicide. Mean vegetative encroachment of cogongrass was < 2 m/yr for both habitat types regardless of burning. Vegetative encroachment was more than double in burned plots compared to unburned plots. Spikelets of cogongrass consistently dispersed farther into pine-bluestem (x¯ =17.3 m) forests compared to pine-shrub forests (x¯ = 9.4 m). Vegetative encroachment was not affected by size of infestation for cogongrass patches on firing points (x¯ < 1 m/yr) (P > 0.643). In areas of soil disturbance from military equipment, vegetative expansion rates of 7-10 m/yr were recorded. There was a positive relationship between military troop use and cogongrass establishment for one of the 2 years of the study (P = 0.023). Growth and establishment of cogongrass did not vary for unpaved roads with differing levels of maintenance and traffic (P > 0.173). Species diversity and abundance of herbaceous vegetation was less in cogongrass infestations compared to uninfested adjacent areas. Complete suppression of cogongrass was not evident for any of three native species tested.