Molecular imprinted polymers (MIPs), prepared to propranolol, were evaluated as solid phase extraction (SPE) phases. Monolithic MIPs were converted to particulate SPE materials by crushing, sieving, and sedimentation to remove fines. A high proportion (85%) of template propranolol was recovered with initial washes of the MIPs. Template leaching diminished with successive washes though low level leachings till compromised analysis of propranolol at trace levels (<5ng/mL). It was possible to retain propranolol on the MIPs following application in aqueous solution, biological matrices and organic solvent. In reversed and normal phase mode it was necessary to include an acid or base to achieve elution of basic compounds. In reversed phase mode, propranolol-imprinted MPs showed a degree of selectivity when using methanol:water:triethylamine but not methanol:water:trifluoroacetic acid elution solvents. This selectivity was related to structure when structurally-diverse compounds were assessed. Selectivity was very limited when assessed using compounds structurally similar to propranolol. In normal phase mode, MIPs retained amino-alcohols more strongly than a non-imprinted polymer though there was no evidence of a structure-related selectivity. A non-imprinted polymer and one prepared to an alternative template (tamoxifen) were assessed as reference polymers by which to assess the selectivity of propranolol-imprinted MIPs. Both materials were suitable references in reversed-phase mode though little selectivity was evident. The non-imprinted polymer was a poor comparator in normal phase mode as it appeared to show lesser non-specific binding than the imprinted MIP. Reversed and normal phase SPE methods were successfully developed for compounds structurally-simila to propranolol from human plasma. A protein precipitation step ahead of SPE was necessary. The MEP-based asays showed no advantage over conventional SPE and solvent extraction assay approaches. Although MIP-based extracts were clean, accuracy was poor at low concentrations (2 and 5 ng/mL) due to interference from template impurities leaching from the MJP.

Thesis (M. Phil.)--University of Hong Kong, 1997. / Includes bibliographical references (leaf 122-142).

Thesis (doctoral)--Freie Universität Berlin, 1980.

Thesis (doctoral)--Ludwig Maximilians-Universität zu München, 1979.

Thesis (M.Phil.)--University of Hong Kong, 1997. / Includes bibliographical references (leaf 122-142) Also available in print.

Made available in DSpace on 2015-12-06T23:47:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2007 / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Diversos estudos tem mostrado que memorias aversivas antigas, ja consolidadas, quando reativadas, retomam em um estado fragil, necessitando de uma nova rodada de consolidacao para que ela tome-se novamente estavel. Este fenomeno e denominado de reconsolidacao, sendo dependente tanto da sintese proteica quanto do sistema noradrenergico. A tarefa da preferencia condicionada pelo lugar e um tipo de condicionamento pavloviano no qual o animal aprende a associar pistas ambientais com uma recompensa, sendo amplamente utilizado para o estudo das propriedades recompensadoras dos alimentos e das drogas de abuso. A amigdala basolateral e uma estrutura relacionada com o aprendizado aversivo, como o condicionamento do medo ao som e ao contexto. Esta estrutura esta tambem envolvida com o aprendizado apetitivo, incluindo a preferencia condicionada pelo lugar. Diversos trabalhos com farmacologia, inativacao e lesao tem mostrado que esta estrutura esta envolvida com a aquisicao, a consolidacao, a expressao e o restabelecimento dessa tarefa. Como a infusao de um inibidor de sintese proteica (anisomicina) na amigdala basolateral bloqueou a reconsolidacao em uma tarefa aversiva, e como esta estrutura tambem esta envolvida na expressao da preferencia condicionada pelo lugar, a primeira parte deste estudo investigou se a infusao da anisomicina bloqueou a reconsolidacao desta tarefa. Os resultados deste estudo mostraram que a anisomicina, quando infundida na amigdala basolateral imediatamente apos a reativacao(teste), nao prejudicou o desempenho dos animais em um teste posterior, indicando que nao houve reconsolidacao dependente de sintese proteica nesta estrutura para a tarefa de preferencia condicionada pelo lugar. A segunda parte deste estudo teve por objetivo verificar se o propranolol, um antagonista &#946;-noradrenergico, quando injetado sistemicamente, bloquearia a reconsolidacao da tarefa de preferencia condicinada pelo lugar induzida pela morfina, uma vez que diversos estudos verificaram que essa droga bloqueia a reconsolidacao, tanto de tarefas aversivas, quanto de apetitivas. Novamente, a adminsitracao sistemica do propranolol apos a reativacao(teste), nao prejudicou o desempenho dos animais, sugerindo que nao houve reconsolidacao dependente do sistema noradrenergico na tarefa de preferencia pelo lugar desta tarefa. Os resultados deste estudo mostraram que a anisomicina, quando infundida na amigdala basolateral imediatamente apos a reativacao (teste), nao prejudicou o desempenho dos animais em um teste posterior, indicando que nao houve reconsolidacao dependente de sintese proteica nesta estrutura para a tarefa de preferencia condicionada pelo lugar. A segunda parte deste estudo teve por objetivo verificar se o propranolol, um antagonista &#946;-noradrenergico, quando injetado sistemicamente, bloquearia a reconsolidacao da tarefa de preferencia condicionada pelo lugar induzida pela morfina, uma vez que diversos estudos verificaram que essa droga bloqueia a reconsolidacao, tanto de tarefas aversivas, quanto de apetitivas. Novamente, a administracao sistemica do propranolol apos a reativacao (teste), nao prejudicou o desempenho dos animais, sugerindo que nao houve reconsolidacao dependente do sistema noradrenergico na tarefa de preferencia pelo lugar / BV UNIFESP: Teses e dissertações

Die vorliegende retrospektive Untersuchung fasst Ergebnisse bei 69 Säuglingen zusammen, die in den Jahren 2010 bis 2014 aufgrund infantiler Hämangiome mit eingetretenen oder drohenden Komplikationen am Universitätsklinikum Würzburg mit dem Betablocker Propranolol oral behandelt wurden. Ergänzend wurden von 45 der 69 Elternpaare nach Therapieende Fragebögen zur subjektiven Einschätzung der Behandlung ihrer Kinder ausgefüllt. Die Ergebnisse dieser Studie zeigen in Zusammenschau mit zahlreichen Publikationen zu diesem Thema, dass Propranolol bei proliferierenden Säuglingshämangiomen eine sichere und hocheffektive Therapie bei gleichzeitig überschaubaren und nur in Einzelfällen gravierenden Nebenwirkungen hat. / This retrospective research sums up the outcome of 69 infants with complicated hemangiomas being treated with beta blocker Propranolol between 2010 and 2014 at Universitätsklinikum Würzburg. In addition a survey about the subjective experiences with the Propranolol therapy was completed by 45 of 69 parents. Like the findings in literature the results of this research show that Propranolol in the treatment of complicated hemangioma in infants is save and highly effective.

El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado. / El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado. / Hematohidrosis is a condition that presents with the excretion of blood from intact skin. Reported cases suggest emotional stress reactions as the most common inciting events. The pathogenesis of the condition is not well understood. We describe a 9-year old boy and his 6-month old half-sister with a history of bleeding episodes from the ears, eyes, and scalp, as well as other sites. Symptoms in both children have shown a positive response to propranolol, with decreased frequency and severity of bleeding. There are no prior reports of siblings with hematohidrosis, suggesting a possible genetic predisposition. / Revisión por pares

1. 1. Psychiatric patients presenting with chronic psychogenic polydipsia are often difficult to treat with standard psychiatric interventions. 2. 2. Pharmacologie intervention was attempted in three patients and was successful in one. 3. 3. One patient had a significant and sustained reduction of water intake while on 160 mg of propranolol. 4. 4. One patient did not improve with either propranolol or captopril while a third patient showed no improvement of serum sodium with demeclocycline nor reduction of water intake with propranolol. 5. 5. The potential mechanisms by which these pharmacologic agents might alter thirst in patients with primary polydipsia are discussed.

Orientador: Marcia Carneiro Valera / Coorientadora: Renata Falchete do Prado / Banca: Eduardo Bresciani / Banca: Mariella Vieira Pereira Leão / Resumo: Os objetivos deste estudo são: 1) Esclarecer a possível associação entre o estresse crônico e a estimulação do Sistema Nervoso Simpático (SNS) e investigar sua interferência no desenvolvimento e progressão da lesão periapical; 2) Avaliar a quantidade de receptores para os neurotransmissores na região periapical; 3) Elucidar uma via farmacológica de modulação inflamatória através do uso de bloqueadores adrenérgicos. Trinta e dois ratos Wistar foram submetidos à modelo animal de lesão periapical através da exposição da cavidade pulpar e em seguida foram aleatoriamente divididos em 4 grupos: sem estresse (NS); estresse + solução salina (SS); estresse + β-bloqueador (Sβ); estresse + α-bloqueador (Sα). Os grupos SS, Sβ e Sα foram submetidos à modelo animal de estresse crônico durante 28 dias e receberam injeções diárias de solução salina, propranolol (β bloqueador adrenérgico) e fentolamina (α bloqueador adrenérgico), respectivamente. Após 28 dias os animais foram eutanasiados e procedeu-se as seguintes análises: a) dos níveis séricos de corticosterona através de Radioimunoensaio; b) histomorfométrica por coloração com hematoxilina e eosina; c) da estrutura óssea periapical através de microtomografia computadorizada (micro-CT); d) expressão de receptores β e α adrenérgicos; e) da atividade osteoclástica através de histoquímica para fosfatase ácida resistente ao tartarato (TRAP). Os resultados obtidos mostram um aumento do nível sérico de corticosterona dos animais do grupo SS send... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The objectives of this study are: 1) To clarify the possible association between chronic stress (CS) and stimulation of the Sympathetic Nervous System (SNS) and to investigate its interference in the development and progression of periapical lesion; 2) To evaluate the amount of receptors for neurotransmitters in the periapical region; 3) To elucidate a pharmacological pathway of inflammatory modulation through the use of adrenergic blockers. Thirty- two Wistar rats were submitted to animal model of periapical lesion through exposure of the pulp cavity and were then randomly divided into 4 groups: no stress (NS); stress + saline solution (SS); stress + β-blocker (Sβ); stress + α-blocker (Sα). The SS, Sβ and Sα groups were submitted to animal model of CS for 28 days and received daily injections of saline solution, propranolol (β blocker adrenergic) and phentolamine (α adrenergic blocker), respectively. After 28 days the animals were euthanized and the following analysis were carried out: a) serum corticosterone levels through Radioimmunoassay; b) histomorphometric by staining with hematoxylin and eosin; c) periapical bone structure through micro computed tomography; d) expression of β and α adrenergic receptors; e) osteoclast activity by histochemistry for tartrate resistant acid phosphatase (TRAP). The results obtained show an increase in the seric corticosterone level of the animals of the SS group being statistically significant compared to the NS group animals (without str... (Complete abstract click electronic access below) / Mestre