The opportunistic pathogen P. aeruginosa is a leading cause of hospital-accquired infections, and is also the primary cause of morbidity and mortality in patients with cystic fibrosis (CF). In this thesis, I describe the identification and characterization of a novel LysR-type transcription regulator (LTTR) of P. aeruginosa named BexR. I show that BexR exhibits reversible ON/OFF bistable expression, which leads to the bistable expression of several genes including one encoding a virulence factor. I present results suggesting that this bistable expression depends on positive feedback of BexR. This work illuminates the simplicity with which a transcription regulatory network can exhibit a complex behavior and generate phenotypic diversity in a clonal population.

Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-10-29T11:30:57Z No. of bitstreams: 1 Maria Madalena Noronha de Vasconcelos.pdf: 2721928 bytes, checksum: a8a3efbe4a9691a7a4da030dbc69c3a1 (MD5) / Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2015-05-30T13:58:49Z (GMT) No. of bitstreams: 1 Maria Madalena Noronha de Vasconcelos.pdf: 2721928 bytes, checksum: a8a3efbe4a9691a7a4da030dbc69c3a1 (MD5) / Made available in DSpace on 2015-05-30T13:58:49Z (GMT). No. of bitstreams: 1 Maria Madalena Noronha de Vasconcelos.pdf: 2721928 bytes, checksum: a8a3efbe4a9691a7a4da030dbc69c3a1 (MD5) / Este estudo analisa dois grandes contextos temporalmente opostos, porém análogos ao modelo da estrutura agrária brasileira. Um diz respeito aos fatores estruturantes do processo de formação territorial e aos problemas relativos à questão fundiária brasileira. O outro está na implementação do Plano Nacional de Reforma Agrária (PNRA), em 1985. Apesar da política de pactuação entre o governo e setores populares, a reação da classe dominante contra o Plano, e os compromissos do Estado com esta classe, restringiu este a um instrumento que tentou melhorar a forma de consolidação das relações capitalistas no campo, sem efetivamente modificar a estrutura agrária que a despeito desse processo, continuou bastante concentrada. O Plano Regional de Reforma Agrária (PRRA) foi oficialmente implantado na Bahia em 1986, respeitando as diretrizes do PNRA. Mas as ações efetivas deste, só tiveram inicio em 1987, quando o Estado criou um aparato burocrático, materializado pela Companhia de Desenvolvimento e Ação Regional (CAR), que juntamente com o INCRA e a Prefeitura de Vitória da Conquista, formaram uma “tríade tutelar” na produção e gestão do espaço do Projeto Amaralina. O Município de Vitória da Conquista em situação de crise estrutural, ocasionada pela perda de subsídios por parte do Governo Federal aos cafeicultores, num acordo institucional, incluiu em 1987, o primeiro projeto de assentamento da Região Sudoeste da Bahia, o Projeto de Assentamento Amaralina. Nos procedimentos metodológicos, utilizouse a pesquisa documental com leituras sobre Reforma Agrária, Estado e os fundamentos teórico-metodológicos da Geografia, tendo como eixo orientador para interpretação dos fenômenos as categorias de análise da geografia ― Território, lugar, região e paisagem ― donde o espaço foi a própria categoria estruturante. Com base nos pressupostos da Pesquisa- Ação procedeu-se reuniões e entrevistas com os assentados e técnicos das instituições que operaram o PRRA. Fundamentada no método dialético através da observação participante estes dois movimentos operados em escalas geográfica ― visto tempo-espacial em seus diferentes tamanhos de realidade ― nos permitiram constatar que a forma de distribuição de terras adotada pelo PNRA/PRRA, com políticas compensatórias, não atingiu os objetivos desse plano que almejava a reprodução econômica nos projetos de assentamentos. O saldo da aplicação de “parcos” investimentos, tanto causou diferenciação no próprio lócus do PA Amaralina como os fragmentou na capacidade produtiva ― devido à orientação para investimento na atividade pecuária ― e nas relações sociais. A situação no assentamento Amaralina e de maneira geral para os pequenos produtores é um contexto de proletarização rural, com renda negativa, baixa produtividade e diminuição das propriedades menores.

La grande majorité des causes tranchées par la Cour fédérale relève du droit de l’immigration. Environ 80% des causes plaidées devant la Cour fédérale sont en matière d’immigration. La plupart des causes qui se rendent à la Cour fédérale aboutissent au renvoi de la personne concernée. La requête en sursis est généralement le dernier recours que la personne peut exercer afin d’éviter ou à tout le moins retarder son renvoi du Canada. Près de 800 de ces requêtes en sursis ont été décidées par la Cour fédérale en 2008. Malgré un si grand nombre de causes et malgré le rôle important que ces requêtes peuvent jouer dans la vie d’une personne, aucun auteur n’a organisé et présenté les règles législatives et jurisprudentielles qui s’appliquent à ces procédures. Aucun livre, article ou commentaire n’a été rédigé sur ce sujet. De même, il n’existe aucun cours d’université ni de formations professionnelles sur les requêtes en sursis. Le droit des sursis consiste exclusivement de la jurisprudence des cours fédérales. Ainsi, on s’attend à ce qu’un avocat prépare une requête en sursis intuitivement. Toutefois, à cause de la nature urgente de cette procédure, il est pratiquement impossible pour un avocat inexpérimenté de se préparer adéquatement et de bien représenter les intérêts de son client. Beaucoup de causes ayant un fort potentiel sont perdues par manque d’expérience de l’avocat ou à cause d’une préparation inadéquate. La jurisprudence émanant de la Cour fédérale relativement aux sursis semble être incohérente et parfois même contradictoire. Ce livre organise, présente et explique de façon claire et concise le droit des sursis. Plus particulièrement, nous examinerons en détail les trois types de sursis – les sursis législatifs, administratifs et judiciaires. Tant les juges que les plaideurs trouveront cet ouvrage de référence utile dans la préparation et l’adjudication des causes. / The vast majority of cases heard and determined by the Federal Court of Canada relate to immigration law; approximately 80% of the cases adjudicated by the Federal Court of Canada are immigration matters. Most immigration cases that reach the Federal Court of Canada eventually result in the individual’s removal. A motion for a stay of removal is generally the last recourse a person can seek in order to avoid or, at least, delay his or her removal from Canada. Nearly 800 such motions were adjudicated by the Federal Court of Canada in 2008. Despite such a considerable number of cases and the important role these proceedings play in a person’s life, no author has ever attempted to organize and present the legislative and jurisprudential rules that govern stays. No books, articles or commentaries have been written to analyze the cases rendered on motions for a stay of removal. No document compiling decisions relating to stay of removal has ever been prepared. Similarly, universities and other institutions do not offer courses or professional development training on this subject. The law relating to stays consists exclusively of cases decided by the Federal Court. A lawyer is expected to prepare a stay motion almost intuitively. Yet, the urgent nature of these proceedings makes it practically impossible for inexperienced counsel to conduct adequate research and properly represent the interests of their client. Hence, many strong cases are lost due to a lack of experience and inadequate preparation. Many excellent lawyers practicing immigration law refuse to introduce such proceedings before the Federal Courts because they are not familiar with the principles governing stays. The law of stays in an immigration context resembles a legal patchwork because the case law is often inconsistent and at times contradictory. This book organizes, presents, and explains, in a clear and concise manner, the law of stays. In particular, this book examines the three types of stays: legislative, administrative and judicial. Judges and practitioners alike will find this quick reference tool very useful when dealing with motions for a stay of removal.

La grande majorité des causes tranchées par la Cour fédérale relève du droit de l’immigration. Environ 80% des causes plaidées devant la Cour fédérale sont en matière d’immigration. La plupart des causes qui se rendent à la Cour fédérale aboutissent au renvoi de la personne concernée. La requête en sursis est généralement le dernier recours que la personne peut exercer afin d’éviter ou à tout le moins retarder son renvoi du Canada. Près de 800 de ces requêtes en sursis ont été décidées par la Cour fédérale en 2008. Malgré un si grand nombre de causes et malgré le rôle important que ces requêtes peuvent jouer dans la vie d’une personne, aucun auteur n’a organisé et présenté les règles législatives et jurisprudentielles qui s’appliquent à ces procédures. Aucun livre, article ou commentaire n’a été rédigé sur ce sujet. De même, il n’existe aucun cours d’université ni de formations professionnelles sur les requêtes en sursis. Le droit des sursis consiste exclusivement de la jurisprudence des cours fédérales. Ainsi, on s’attend à ce qu’un avocat prépare une requête en sursis intuitivement. Toutefois, à cause de la nature urgente de cette procédure, il est pratiquement impossible pour un avocat inexpérimenté de se préparer adéquatement et de bien représenter les intérêts de son client. Beaucoup de causes ayant un fort potentiel sont perdues par manque d’expérience de l’avocat ou à cause d’une préparation inadéquate. La jurisprudence émanant de la Cour fédérale relativement aux sursis semble être incohérente et parfois même contradictoire. Ce livre organise, présente et explique de façon claire et concise le droit des sursis. Plus particulièrement, nous examinerons en détail les trois types de sursis – les sursis législatifs, administratifs et judiciaires. Tant les juges que les plaideurs trouveront cet ouvrage de référence utile dans la préparation et l’adjudication des causes. / The vast majority of cases heard and determined by the Federal Court of Canada relate to immigration law; approximately 80% of the cases adjudicated by the Federal Court of Canada are immigration matters. Most immigration cases that reach the Federal Court of Canada eventually result in the individual’s removal. A motion for a stay of removal is generally the last recourse a person can seek in order to avoid or, at least, delay his or her removal from Canada. Nearly 800 such motions were adjudicated by the Federal Court of Canada in 2008. Despite such a considerable number of cases and the important role these proceedings play in a person’s life, no author has ever attempted to organize and present the legislative and jurisprudential rules that govern stays. No books, articles or commentaries have been written to analyze the cases rendered on motions for a stay of removal. No document compiling decisions relating to stay of removal has ever been prepared. Similarly, universities and other institutions do not offer courses or professional development training on this subject. The law relating to stays consists exclusively of cases decided by the Federal Court. A lawyer is expected to prepare a stay motion almost intuitively. Yet, the urgent nature of these proceedings makes it practically impossible for inexperienced counsel to conduct adequate research and properly represent the interests of their client. Hence, many strong cases are lost due to a lack of experience and inadequate preparation. Many excellent lawyers practicing immigration law refuse to introduce such proceedings before the Federal Courts because they are not familiar with the principles governing stays. The law of stays in an immigration context resembles a legal patchwork because the case law is often inconsistent and at times contradictory. This book organizes, presents, and explains, in a clear and concise manner, the law of stays. In particular, this book examines the three types of stays: legislative, administrative and judicial. Judges and practitioners alike will find this quick reference tool very useful when dealing with motions for a stay of removal.