Zweck der molekularen Bildgebung ist es, biologische Prozesse auf zellulärer und molekularer Ebene zu messen und zu charakterisieren, um so die Ursachen von Krankheiten und Veränderungen im Organismus zu diagnostizieren. Sie basiert auf dem Einsatz molekularer Bildgebungssonden, welche einen spezifischen biologischen Vorgang darstellen oder sich spezifisch in dem zu untersuchenden Gewebe anreichern oder aktiviert werden. Ziel dieser Arbeit war die Entwicklung und Analyse neuer Bildgebungssonden für die spezifische in-vivo-Bildgebung der Apoptose und von Enzymaktivitäten mittels Magnetresonanztomographie (MRT) auf der Grundlage sehr kleiner Eisenoxidnanopartikel (very small iron oxide particles, VSOP). VSOP sind superparamagnetisch und durch ihre negativ geladene Citrathülle elektrostatisch stabilisiert. Für die Apoptose-Bildgebung sollte durch Bindung des Proteins Annexin A5 (AnxA5) an die Citrathülle der VSOP eine zielgerichtete Sonde hergestellt werden (AnxA5-VSOP). Für die Bildgebung von Enzymaktivitäten sollte eine durch die Matrixmetalloproteinase-9 (MMP-9) aktivierbare Sonde hergestellt werden (Protease-spezifische Eisenoxidpartikel, PSOP). / The goal of molecular imaging is to characterize and measure biological processes at cellular and molecular levels for the purpose of diagnosing the cause of diseases and molecular abnormalities. Molecular imaging is based on the use of probes with a high affinity to the target tissue and / or which are specifically activated. The aim of this study was to develop and analyze new molecular imaging probes for the in vivo imaging of apoptosis and enzyme activity using magnetic resonance imaging (MRI), based on very small iron oxide particles (VSOP). VSOP are superparamagnetic and electrostatically stabilized due to their negatively charged citrate surface. For the imaging of apoptosis the protein annexin A5 (AnxA5) was coupled to the citrate surface (AnxA5-VSOP). For the imaging of enzyme activities an activatable imaging probe with a cleavage site for the matrix metalloproteinase 9 (MMP-9) was synthesized (protease-specific iron oxide particles, PSOP).

Submitted by Cássio da Cruz Nogueira (cassionogueirakk@gmail.com) on 2017-03-06T13:41:48Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_ProgramaProducaoSustentavel.pdf: 2564763 bytes, checksum: 16b735771e9c7a3b1f5e8a6088120e03 (MD5) / Approved for entry into archive by Edisangela Bastos (edisangela@ufpa.br) on 2017-03-08T13:44:56Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_ProgramaProducaoSustentavel.pdf: 2564763 bytes, checksum: 16b735771e9c7a3b1f5e8a6088120e03 (MD5) / Made available in DSpace on 2017-03-08T13:44:56Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_ProgramaProducaoSustentavel.pdf: 2564763 bytes, checksum: 16b735771e9c7a3b1f5e8a6088120e03 (MD5) Previous issue date: 2016-07-13 / CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico / No estado do Pará, a cultura do dendê vem apresentando elevadas taxas anuais de crescimento da produção, no período de 1990 a 2012 o estado alcançou uma taxa de variação média anual de 5%. Essa expansão do dendê na Amazônia se deve ao Programa Federal de Produção Sustentável de Palma de Óleo (PSOP), criado para fomentar e direcionar a produção do dendê na região. Diante da relevância que o campesinato apresenta e da expansão do dendê na região, objetiva-se avaliar os impactos que o PSOP está ocasionando na Trajetória Camponesa.T1 do município do Acará e se, por ventura, ocasionou em uma mudança na lógica produtiva peculiar do camponês local. Como resultados a pesquisa conclui que existem evidências de que o PSOP possa impactar a trajetória tecnológica T1 do município do Acará, promovendo um processo de substituição das atividades desenvolvidas pelo camponês. A escassez de dados oficiais e de trabalhos voltados à avaliação dos impactos do PSOP evidenciam a necessidade de realização de estudos dessa temática como forma de subsidiar a elaboração de políticas públicas mais adequadas ao contexto amazônico. / In the state of Pará, the palm culture has shown high annual growth rates from 1990 to 2012 the state has achieved an average annual production growth rate of 5%. This expansion of palm oil in the Amazon is due to the Federal Sustainable Production Program of Oil Palm (PSOP), created to foster and direct the production of palm oil in the region. Given the importance that the peasantry has and the expansion of palm oil in the region, aims to evaluate the impact that the PSOP is causing the Trajectory Camponesa.T1 the municipality of Acará and if, perchance, resulted in a change in the peculiar logic of productive local farmer. As a result the research concludes that there is evidence that the PSOP may impact the technological trajectory of Acará county T1, promoting a process of replacing the activities developed by the peasant. The lack of official data and studies related to the evaluation of PSOP impacts highlight the need for studies of this theme as a way to support the most appropriate public policy development to the Amazon region.