5 mutations responsables de retard mental ont été identifiées dans le gène p21-activated kinase 3 (pak3). Nous avons récemment identifiés dans pak3 deux exons alternatifs très conservés appelés b et c. Ainsi, en plus du variants PAK3a (dépourvu des inserts b ou c), le gène pak3 code pour 3 nouveaux variants d'épissage PAK3b, PAK3c et PAK3cb qui sont constitutivement actifs et insensibles aux GTPases. De plus, contrairement à PAK1 et PAK3a, leur domaine d'auto-inhibition est incapable d'inhiber un domaine kinase. Ainsi, le but de ce projet était de comprendre le mécanisme de régulation de la kinase PAK3. Un modèle de régulation a récemment été proposé dans lequel PAK1 forme des homodimères pouvant être dissociés par les GTPases, permettant ainsi l'activation de la kinase. En se basant sur ces observations j'ai cherché à identifier les dimères PAK3 et j'ai montré que les kinases PAK3a, b, c et cb forment préférentiellement des hétérodimères avec PAK1. J'ai démontré l'existence de ces dimères dans le cerveau et j'ai mis en évidence que ces hétérodimères permettent à chaque monomère de réguler l'activité kinase de son partenaire in vitro. Ce travail permet de proposer un modèle de régulation symétrique pour PAK3a qui forme des hétérodimères avec PAK1 et un nouveau modèle de régulation asymétrique pour les variants d'épissage, également basé sur leur hétérodimérisation avec PAK1. Mes résultats montrant une corégulation des kinases PAK neuronales suggèrent d'une part que leur activation puisse être synchronisée et d'autre part que dans certaines situations physiopathologiques (Cancer et maladies neurologiques) leur dérèglement puissent interférer.

Die vorliegende Dissertation berichtet über die Untersuchung von selbst-aggregierten Einfach- und Mehrfachschichten aus Nanographenen-Derivate mit Hilfe der Rastertunnelmikroskopie (RTM) an Fest-Flüssig-Grenzflächen. Die -Konjugation bringt einzigartige elektronische Eigenschaften mit sich, so dass die Nanographen-Derivate viel versprechende Bausteine für eine molekulare und organische Elektronik sind, da sie maßgeschneidert und kostengünstig prozessiert werden können, und leicht und flexibel sind. Für elektronische Anwendungen ist es notwendig, die Nanographene in ultradünnen Filmen mit geordneten supramolekularen Strukturen zu organisieren. Nanostrukturen werden für Nanographene-Derivate auf hoch orientiertem pyrolytischem Graphit (HOPG) untersucht, wie zum Beispiel alkylierte Hexi-peri-hexabenzocoronene (HBCs) unterschiedlicher Symmetrie und dreiecksförmige polyzyklische aromatische Kohlenwasserstoffe (PAK). Es zeigt eine erstaunliche Vielfalt von supramolekularen Strukturen, z.B. Zick-Zack-, Blumen- oder Honigwaben-Muster. Eine faszinierende Besonderheit besteht in den Honigwaben Strukturen, die sich durch Selbstaggregation dreieckiger alkylierter Phenyl-PAKs bilden, und die damit Nanotemplate für Gastmoleküle darstellen. In vielen Fällen bilden Nanographene-Derivate nicht nur Monoschichte sondern auch Multischichten auf Graphit. Die Selbstorganisation von Doppelschichten aus einer HBC-Stern-Verbindung bietet das Potenzial für Baustelemente in der organischen Elektronik, zum Beispiel für Nanodrähte. Die alkylierten Phenyl-HBCs bilden polykristalline Strukturen sowohl in der "face-on"-Anordnung in Monoschichten auf Graphit wie in der "edge-on"-Anordnung in Multischichten, die sich in einem äußeren elektrischen Feld bilden. Beides kann nützlich sein, da für die mögliche Anwendung in einer Photovoltaik-Zelle die "face-on"-Orientierung auf Oberflächen erforderlich ist, während für organische Feldeffekt-Transistoreneine "edge-on" Nanostruktur benötigt wird. / This thesis reports on the investigation of self-assembled mono- and multilayers from nanographene derivatives via scanning tunneling microscopy (STM) at solid-liquid interfaces. Because of the unique electronic properties associated with their -bonded topology, nanographenes are promising building blocks for molecular and organic electronics, which provide the possibility of tunability together with low-cost processing, light weight, and flexibility. For the application in electronics it is necessary to organize nanographenes in ultrathin films with well-ordered supramolecular structures. Nanostructures of monolayers on Highly Oriented Pyrolytic Graphite (HOPG) are studied for different nanographene derivatives, such as alkylated hexa-peri-hexabenzocoronenes (HBCs) with different symmetries, and triangle-shaped polycyclic aromatic hydrocarbons (PAHs). They exhibit a surprising diversity of supramolecular structures, for example zigzag, flower-like or honeycomb shapes. A fascinating peculiarity provides the honeycomb structures which are self-assembled from triangle-shaped alkylated phenyl PAHs, which provide nanotemplates to accommodate guest molecules. In many cases, nanographene derivatives not only form monolayers but also multilayers on HOPG. Star-shaped HBC molecules self organize into bilayers in polar solvents, which exhibit the potential for the formation of building blocks of organic electronics, for instance nanowires. The alkylated phenyl HBCs form polycrystalline structures both in the “face-on” arrangement in a monolayer on HOPG, and “edge-on” in multilayers within an external electric field. Both may be useful for potential applications, since in a photovoltaic cell, the “face-on” orientation on surfaces is required, while for the purpose to be applied in organic field-effect transistors, the “edge-on” nanostructure on the electrodes is necessary.

Background: For companies operating globally, expatriates represent a knowledge resource. When different ways of thinking, different experiences and ideas meet, new ideas and knowledge are created. Managers who have lived and worked abroad make an essential addition to the range of ideas and experiences that a company can make use of. While going on an international assignment offers the opportunity to acquire knowledge, finishing one, and moving on, offers the opportunity to transfer this knowledge and utilize it in the organization. Purpose: The purpose of this study is to examine how a global corporation utilizes the knowledge its expatriates gain during international assignments. Realization: We have looked at utilization of expatriates’ knowledge at Tetra Pak from both the HR managers’ and the expatriates’ point of view. We conducted five interviews with HR managers and sent out questionnaires to 60 expatriates, of which 21 replied. Conclusion: Knowledge management is highly topical at Tetra Pak right now. To open up for learning and to spread knowledge are priorities. Utilization of expatriates’ knowledge can be looked at on two levels, the individual- and the organizational level. On the individual level, it is very much a question of career management. On the organizational level, it is a matter of knowledge transfer and sharing. Transfer of knowledge in general is very much intended at Tetra Pak, but there is no focus on expatriates’ knowledge. Knowledge sharing on the other hand, seems to more or less “just happen”, it is a matter of learning by accident rather than intent.

Background: In today’s business climate, a growing number of corporations have chosen to explore markets outside their national boundaries. There has been a shift in marketing strategies from a domestic perspective to a global one. Of all the methods available for firms to internationalize, Foreign Direct Investment possesses several advantages; it stimulates employment; raises wages, and replaces declining market sectors. It acts as a stimulant for infrastructure development and technology transfer. For Sweden Saudi Arabia is the most important export market in the Middle East. As Saudi Arabia is a country that presents both huge business opportunities and challenges for Swedish firms, it is important to study how some firms have succeeded in entering this market and what attracted them there in the first place. Purpose: The author’s intent is to identify the advantages and disadvantages for Swedish firms of carrying out FDI in Saudi Arabia. Method: this thesis is based on a case study of two Swedish firms with operations in Saudi Arabia. The author has chosen to use a qualitative research method. Empirical data was gathered by e-mails and phone interviews. Conclusions:  To author answers the research questions; Why did Swedish firms decide to establish themselves in Saudi Arabia? & How did they manage to establish themselves through FDI in that region?  Swedish firms get into the Saudi market because of advantages related the economy, the considerable market size and revenue, improved business climate, business opportunities, their global marketing strategy and their ownership specific advantages. As for how they succeeded to establish their FDI, it was through an incremental approach aided by a good knowledge of the market, the ability of the firm, proactive steps to reduce the impact of cultural differences and the country of origin effect. Suggestions for future research: Future research could focus on doing broader studies involving a larger sample, focusing on one or a few FDI determinants to investigate how they affect the investment decision as well as the managerial implications of cultural distance.

<p>Background: For companies operating globally, expatriates represent a knowledge resource. When different ways of thinking, different experiences and ideas meet, new ideas and knowledge are created. Managers who have lived and worked abroad make an essential addition to the range of ideas and experiences that a company can make use of. While going on an international assignment offers the opportunity to acquire knowledge, finishing one, and moving on, offers the opportunity to transfer this knowledge and utilize it in the organization. </p><p>Purpose: The purpose of this study is to examine how a global corporation utilizes the knowledge its expatriates gain during international assignments. </p><p>Realization: We have looked at utilization of expatriates’ knowledge at Tetra Pak from both the HR managers’ and the expatriates’ point of view. We conducted five interviews with HR managers and sent out questionnaires to 60 expatriates, of which 21 replied. </p><p>Conclusion: Knowledge management is highly topical at Tetra Pak right now. To open up for learning and to spread knowledge are priorities. Utilization of expatriates’ knowledge can be looked at on two levels, the individual- and the organizational level. On the individual level, it is very much a question of career management. On the organizational level, it is a matter of knowledge transfer and sharing. Transfer of knowledge in general is very much intended at Tetra Pak, but there is no focus on expatriates’ knowledge. Knowledge sharing on the other hand, seems to more or less “just happen”, it is a matter of learning by accident rather than intent.</p>

published_or_final_version / Architecture / Master / Master of Architecture

Vascular smooth muscle cell migration is a significant contributor to many aspects of heart disease, and specifically atherosclerosis. Tissue damage in the arteries can result in the formation of a fatty streak. Smooth muscle cells (SMC) can then migrate to this site to form a fibrous cap, stabilizing the fatty plaque. Since cardiovascular disease is the leading cause of death in developed countries, this function of SMC is an essential area of study. The formation of lamellipodia and circular dorsal ruffles were studied in this project as indicators that cell migration is occurring. The roles of the proteins p53, Rac, caldesmon and PTEN were investigated with regards to these actin-based structures. The tumour suppressor p53 is often reported to cause apoptosis, senescence or cell cycle arrest when stress is placed on a cell, but has recently been shown to regulate cell migration as well. It was determined in this project that p53 could inhibit the formation of both lamellipodia and circular dorsal ruffles. It was also shown that this could occur directly through an inhibition of the GTPase Rac. Previous studies have shown that p53 can upregulate caldesmon, a protein which is known to bind to and stabilize actin filaments while inhibiting Arp2/3-mediated branching. It was confirmed that p53 could upregulate caldesmon, and that caldesmon could inhibit the formation of lamellipodia and circular dorsal ruffles. The phosphorylation of caldesmon by p21-associated kinase (PAK) or extracellular signal-related kinase (Erk) was shown to effectively reverse the ability of caldesmon to inhibit these structures. The role of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) was also studied with regards to this signalling pathway. PTEN was shown to inhibit lamellipodia and circular dorsal ruffles through its lipid phosphatase activity. It was concluded that p53 can inhibit the formation of lamellipodia and circular dorsal ruffles in vascular SMC, and that this occurs through Rac, caldesmon and PTEN. / Thesis (Master, Biochemistry) -- Queen's University, 2011-06-10 13:15:37.081

Thesis (M. Phil.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 125-128).

Thesis (M.S. in Physics)--Naval Postgraduate School, January 1964. / Thesis Advisor(s): Hawns, William W. "January 1964." Description based on title screen as viewed on June 2, 2010 DTIC Descriptor(s): (Neutron Flux), (Research Reactors, Measurement, Neutron Activation, Reactor Lattice Parameters, Reactor Control, Power, Reactor Operation, Reactor Power Density, Scintillation Counters, Foils (Materials), Gold, Gamma Rays, Nuclear Radiation Spectrometers, Indium, Monitors, Errors, Probability, Statistical Analysis. DTIC Identifier(s): AGN-201 Reactors. Includes bibliographical references (p. 20). Also available in print.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第21840号 / 農博第2353号 / 新制||農||1069(附属図書館) / 学位論文||H31||N5212(農学部図書室) / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 田中 千尋, 教授 本田 与一, 准教授 刑部 正博 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM