Temmincks pangolins are the world’s most trafficked mammal. This animal is unique to Southern Africa, and just as rare are the conservationists who are working against all odds to save this species from extinction. While the incidence of poaching within South Africa is increasing, as is the value of a pangolin, the conservation workspace is rapidly becoming increasingly dangerous. In light of the above, one cannot help but be concerned for the well-being of the pangolin conservationists. Despite engaging in meaningful work, the conservationists are exposing themselves to prolonged stress that is likely to impact their well-being. The purpose of this study is to explore the well-being of Temmincks pangolin conservationists in terms of state and trait depression as well as burnout. Furthermore, the study aims to understand whether coping mechanisms or resilience mitigate the effect of the potential depression and burnout. Therefore, a sequential explanatory mixed-method design was adopted for this study. The quantitative data was gathered using the Maslach Burnout Inventory General Survey (MBI-GS), the State Trait Personality Inventory Form Y (STPI-Y) depression subscale, the Coping Orientations to Problems Experienced (COPE) questionnaire and the Predictive 6 Factor Resilience Scale (PR6). The qualitative data was collected utilising a semi-structured interview. Results indicated that the participants were currently experiencing both state and trait depression. Additionally, a third of the sample is at risk of developing burnout. In spite of this, the participants displayed the presence of goal focus, tenacity and collaborative spirit. These elements of resilience were shown to assist the conservationists to persist through adversity. The participants also showed the use of effective coping mechanisms, namely; accepting, planning and critically engaging with perceived stressors. These coping mechanisms were shown to enhance the presence of the resilience indicators. Thus, despite depression and burnout impacting negatively on their well-being, the participants are utilising coping mechanisms and resilience which enhances their well-being. Recommendations included a developmental workshop focusing on fostering effective coping mechanisms to build stronger resilience and ward off potential burnout and possibly ameliorate current depression experiences. / Dissertation (MA (Psychology))--University of Pretoria, 2020. / Psychology / MA (Psychology) / Unrestricted

Wolbachia are widely spread, maternally transmitted insect endosymbiotic intracellular bacteria. They have been implicated in the control of several insect transmitted diseases, including dengue, yellow fever, Zika and malaria. Effective pathogen suppression in the insect host is shown to be proportional to the intracellular levels of bacteria. Therefore, understanding the molecular mechanisms underlying Wolbachia accumulation within organisms is extremely important for future epidemic control and research. Using Drosophila as a model insect, our lab has previously observed Wolbachia tropism to stem cell niches. Current work has identified polar cells as an additional site of Wolbachia tropism and demonstrated that Wg/Wnt signaling is important for Wolbachia intracellular accumulation in these somatic cells. In this thesis, we first observed that the Wg/Wnt pathway protein Armadillo also controls Wolbachia levels in the germline cells, indicating the possibility of having a conserved molecular mechanism controlling Wolbachia. Using RNAi and small molecule inhibitors of Shaggy, another component of the canonical Wg/Wnt pathway, we demonstrate that the canonical Wg/Wnt signaling is essential for Wolbachia intracellular accumulation. Our investigation provides fundamental insights into the mechanisms of Wolbachia intracellular accumulation. Furthermore, it offers novel strategies to modulate Wolbachia in non-model insect species, including various disease transmitting Anopheles, Culex, and Aedes. These findings potentially will increase the effectiveness of a Wolbachia-based vector transmitted disease suppression. / 2017-02-28

The aim of this work was to evaluate international trade with Manis. spp. according to the CITES database during the period 2002-2013. Manis spp. was classified in appendix II CITES in 1995. In 2000 there was an unsuccesfull attempt to reclassified Manis spp. to appendix I CITES. In this year the zero quota was set for Asian pangolins for commercial purpose. A rapid increase of an illegal trade was noticed after the setting of zero quota. The most frequent trade with pangolins was in these forms: 1) derivatives, 2) specimens, 3) scales, 4) live pangolins and 5) skins. Asian pangolins´ species were traded mostly in forms of derivatives, specimens, scales and skins in the period 2002-2013. M. javanica and M. pentadactyla with medium and large distribution area were quantitatively the most traded species, respectively. Main importers of Asian forms of pangolins were: USA (derivatives and specimens), China (scales in kg) and Indonesia (scales in pieces) and Mexico (skins). The African pangolin M. tricuspis was the third most frequent species in the international trade and was traded especially in form of live pangolins. M. tricuspis lives in one of the largest distribution area. Main importer of live pangolins was Italy. Breeding pangolins in captivity is due to the specific feeding requirements demanding. There are about 46 individuals of pangolins in Zoos all over the world, whereas in Europe there are only 2 pangolins in the Leipzig Zoo.