La propagation d’un incendie dans un espace clos s’explique par l’inflammation de matières combustibles. Un cas important est celui de la propagation d’une flamme sur une paroi verticale. En effet si la flamme progresse dans le même sens que l’écoulement (cas co-courant), la croissance est rapide. Dans ce cas, l’émission des vapeurs combustibles (pyrolyse) et le dégagement de la chaleur apportée par la combustion sont couplés par les flux convectés et rayonnés à la paroi. Ces flammes de paroi verticale sont pilotées par les forces de flottabilité, et se caractérisent par un régime de basse vitesse et avec une forte production de suie. Bien que de nombreux travaux aient été consacrés à l’étude des flammes de paroi verticale [1-3], peu d’entre eux ont été dédiés à l’étude de l’écoulement dans la couche limite proche de la paroi et à l’étude des zones de production de suie, lesquels sont des données nécessaires pour la validation des codes de calcul. Pour cela, des mesures simultanées de vitesse par PIV et de concentration de suie par LII ont été réalisées sur un brûleur gaz en configuration paroi-verticale. Dans un premier temps, ces mesures ont permis l’analyse de la forme, de la taille et de la concentration des zones de formation de suies (poches de suie) à différentes hauteurs dans la flamme. Ensuite, les champs 2D de vitesses moyennes (horizontales et verticales) ont été étudiés, ainsi que leurs fluctuations (densités de probabilité et écart-type). Une description de la couche limite réactive, à l’aide d’une échelle caractéristique obtenue avec des mesures de vitesse plus résolues spatialement (PIV « zoomé »), a également été réalisée. Finalement, les mesures de LII et PIV couplées ont permis d’étudier l’influence du champ de vitesse sur la distribution des suies dans la flamme, ainsi que le transport et le flux turbulent de la fraction volumique de suie dans la couche limite réactive. / The fire growth and spread on a confined space depends on the inflammation and combustion of combustible materials. An important case is the fire propagation on a vertical wall configuration, in which the pyrolysis gas and the total heat flux released by the flame are coupled by convective and radiative heat flux from the flame to the wall. This kind of flame is piloted by the buoyancy forces, and is characterized by a low velocity regime and a strong generation of soot particles. Although numerous works have been devoted on the study of vertical wall flames, few have been carried out on the analysis of the flame within the reactive boundary layer and the study of the zones of production of soot particles, which is data necessary for fire simulation codes validation. In this aim, simultaneous measurements of velocity by Particle Image Velocity (PIV) and of soot volume fraction by planar laser induced incandescence (LII) have been carried out on vertical wall fire generated by a vertical porous burner fed with a CH4/C2H4 mixture. First, the characteristics of soot sheet (shape, size, thickness, and peak concentration) have been studied at different heights into the flame, as well as the average and RMS soot volume fraction fields. Then, average and RMS fields of velocity and their probability density function have been analyzed. A description of the reactive boundary layer, through the definition of a characteristic velocity scale in the near-wall zone (viscous sub-layer), has been carried out by using a « PIV Zoom » set-up. Finally, simultaneous LII/PIV measurements have been carried out in order to study the influence of the aerodynamics of the flow on the soot volume fraction distribution, as well as the transport and turbulent flux of soot into the reactive boundary layer.

Paper-fluidic devices are a popular platform for point-of-care diagnostics due to their low cost, ease of use, and equipment-free detection of target molecules. The most common example is the lateral flow assay, in which samples are added to a paper membrane and a colorimetric indicator provides a binary signal indicating whether the molecule of interest is present. A novel lateral flow assay was developed to detect a protein biomarker for early stage cervical cancer. Cervical cancer can be cured if detected and treated at an early stage, but approximately 90% of cervical cancer deaths occur in low and middle-income countries due to lack of accessible testing. Methods for detecting the biomarker, valosin-containing protein (VCP), were optimized using enzymatic and gold nanoparticle dot blots, then lateral flow assays were developed and validated using purified VCP and cervical cancer HeLa cells. Future validation with patient tissue samples will permit translation of this device to testing clinics in low-resource areas. Despite advantages for use in resource limited settings, lateral flow assays are limited by their inability to perform more complex or multi-step processes, such as nucleic acid amplification or enzymatic signal enhancement. Thermally actuated wax valves are one mechanism that provides complete control over fluid obstruction and release. To better understand how wax valves can be used in fully automated, self-contained lateral flow assays, different sizes and geometries of valves were tested to investigate their effects on actuation time, flow rate, and flow pattern. Another limitation in the understanding of lateral flow assays is the lack of experimental data describing the microscale flow within the pores of the paper membrane that drives the biophysical reactions in the assay. Mathematical models can be designed to explain macroscopic phenomena, but so far, no literature has compared microfluidic models to microfluidic data. To quantify microfluidic properties within lateral flow assays, fluorescent nanoparticles were imaged flowing through different areas of the membrane and their velocity was quantified using micro-particle image velocimetry (µPIV). Scanning electron microscope images were used to verify that this experimental model was reasonable for describing microfluidic properties of the lateral flow assay. Altogether, this document investigates how developing lateral flow assays for cervical cancer detection can save lives by improving the accessibility of an early diagnosis, and how more robust lateral flow assay characterization can expand their applicability to a broad range of detection processes.

Oscillating-foil hydrokinetic turbines have gained interest over the years to extract energy from renewable sources. The influence of the sweep angle on the performance of a fully-passive oscillating-plate hydrokinetic turbine prototype was investigated experimentally in the present work. The sweep angle was introduced to promote spanwise flow along the plate in order to manipulate the leading edge vortex (LEV) and hydrodynamically optimize the performance of the turbine. In the present work, flat plates of two configurations were considered: a plate with a 6° sweep angle and an unswept plate (control), which were undergoing fully-passive pitch and heave motions in uniform inflow at the Reynolds numbers ranging from 15 000 to 30 000. The resulting kinematic parameters and the energy extraction performance were evaluated for both plates. Planar (2D) particle image velocimetry (PIV) was used to obtain patterns of the phase-averaged out-of-plane vorticity during the oscillation cycle. The circulation in the wake was then related to the induced-forces on the plate by calculating the moments of vorticity of the LEV with respect to the pitching axis of the plate. Tomographic (3D) PIV was implemented in evaluating the influence of the spanwise flow on the dynamics of the vortex structure in three-dimensional space. The rate of deformation of the vortex length was quantified by calculating the deformation terms embedded in the vorticity equations, then linked to the stability of the vortex. The results show evidence of delay of the shedding of LEV and increased vortex stability, in the case of the swept plate. The manipulation of the LEV by the spanwise flow was related to the induced kinematics exhibited by the prolonged heave forces experienced by the swept plate, which led to the higher power extraction performance at high inflow velocities. In the presence of spanwise flow, positive vortex stretching along the vortex line increased the stabilization of the vortex core and prevented the onset of helical vortex breakdown, observed in the case of the unswept plate. The use of the sweep profile on the plate has led to the improvement of energy extraction performance of the fully-passive hydrokinetic turbine. / Graduate

Für die Untersuchung von Luft/Wasser-Strömungen wurde ein horizontaler Acrylglas-Kanal mit rechteckigem Querschnitt gebaut. Der Kanal ermöglicht Gleich- und Gegenstrom-Versuche bei Atmosphärendruck, insbesondere die Untersuchung der Schwallströmung. Es wurden optische Messungen mit einer Hochgeschwindigkeits-Kamera durchgeführt, die durch synchronisierte dynamische Druckmessungen ergänzt wurden. Für die Analyse der Bilder wurde eine Methode zur Erfassung der Phasengrenze entwickelt und diese anhand möglicher Anwendungen getestet. Die Druckmessungen zeigten, dass der Druck bei Schwallströmungen um einige Kilopascal ansteigt und wieder abfällt, sobald der Schwall aus dem Kanal austritt. Zudem wurden Geschwindigkeiten in der flüssigen Phase mittels nicht invasiver Verfahren gemessen. Das durchschnittliche Geschwindigkeits-Profil am Kanaleintritt wurde mit Ultraschall-Köpfen bestimmt. Die Ermittlung des Geschwindigkeitsfeldes in einem Schwall erfolgte mit PIV (Particle Image Velocimetry).

This thesis deals with turbulent flows in 90 degree curved pipes of circular cross-section. The flow cases investigated experimentally are turbulent flow with and without an additional motion, swirling or pulsating, superposed on the primary flow. The aim is to investigate these complex flows in detail both in terms of statistical quantities as well as vortical structures that are apparent when curvature is present. Such a flow field can contain strong secondary flow in a plane normal to the main flow direction as well as reverse flow. The motivation of the study has mainly been the presence of highly pulsating turbulent flow through complex geometries, including sharp bends, in the gas exchange system of Internal Combustion Engines (ICE). On the other hand, the industrial relevance and importance of the other type of flows were not underestimated. The geometry used was curved pipes of different curvature ratios, mounted at the exit of straight pipe sections which constituted the inflow conditions. Two experimental set ups have been used. In the first one, fully developed turbulent flow with a well defined inflow condition was fed into the pipe bend. A swirling motion could be applied in order to study the interaction between the swirl and the secondary flow induced by the bend itself. In the second set up a highly pulsating flow (up to 40 Hz) was achieved by rotating a valve located at a short distance upstream from the measurement site. In this case engine-like conditions were examined, where the turbulent flow into the bend is non-developed and the pipe bend is sharp. In addition to flow measurements, the effect of non-ideal flow conditions on the performance of a turbocharger was investigated. Three different experimental techniques were employed to study the flow field. Time-resolved stereoscopic particle image velocimetry was used in order to visualize but also quantify the secondary motions at different downstream stations from the pipe bend while combined hot-/cold-wire anemometry was used for statistical analysis. Laser Doppler velocimetry was mainly employed for validation of the aforementioned experimental methods. The three-dimensional flow field depicting varying vortical patterns has been captured under turbulent steady, swirling and pulsating flow conditions, for parameter values for which experimental evidence has been missing in literature. / QC 20120425

Cardiovascular diseases (CVD) remain one of the leading causes of deaths worldwide. The formation and presence of aneurysm is a very important question in the study of this CVDs. An aneurysm is a balloon-like bulge on a blood vessel which forms over time. An aneurysm is usually considered to be a result of weakening of the blood vessel walls, this definition has stood over many years without being conclusively proven. Eventually, the aneurysm could clot or burst due to degradation of the aneurysm wall and accumulation of blood. The latter would lead to internal bleeding and result in a stroke. Local hemodynamics have been found to be very important in the study of the evolution of an aneurysm. In this study, a steady flow experimental investigation was conducted using planar Particle Image Velocimetery (PIV) on a rigid flow phantom of an idealised geometry consisting of a curve parent artery and a spherical aneurysm located on the outer convex side of the curvature. The flow phantom was fabricated directly using a commercially available desktop Stereolithography (STL) 3D printer instead of the more conventional investment casting method using a core. Although 3D printing technologies have been around for many years, the fabrication of flow phantoms by direct printing is still largely under-explored. This thesis details the results of investigation into the optimal printing and post-printing procedures required to produce a flow phantom of suitable clarity and transparency. Other important areas of concern such as the geometric accuracy, surface topography and refractive index of the final model are also investigated. A planar PIV is conducted to study the impact of flow rates on the local flow field in and around the aneurysm and their impact on the wall shear stress. It was found that direct 3D printing is appropriate for the fabrication of flow phantoms suitable for PIV or other flow visualisation techniques. It reduces the complexities and time needed compared to the conventional investment casting methods. It was observed that the optical properties of the printed material such as the high refractive index (RI) and the transmittivity of light could cause a problem in large models. From the PIV measurements it was found that flow rates affect the flow field in both the parent artery and the aneurysm. First, high velocities were observed on the outer curvature of the parent artery. Secondly the centre of rotation in the aneurysm is not at the geometric centre but is displaced slightly in the direction of the flow. Finally, the flow rate affects the angle in which flow enters the aneurysm from the parent vessel. This change in the flow angle affects the flow within the aneurysm. A higher flow rate in the parent artery increases the incident angle which brings the centre of rotation closer to the geometric centre of the aneurysm, this changes the location and magnitude of high velocities and hence the local wall shear stress (WSS) on the wall of the aneurysm. This may have implications in the evolution of aneurysms. / Mechanical and Industrial Engineering

The interaction between a bluff body and the impinging fluid flow, can involve detached boundary layers, massive flow separations, free shear layers, development of recirculation zones and formation of a highly disturbed and complex region downstream of the bluff body, which can be categorized as wake. The present research aims to experimentally investigate such fluid-structure interaction and provide insight into the wake structure of two bluff bodies. To this end, the airwake over the helicopter platform of a Canadian Coast Guard (CCG) polar icebreaker was studied using high-speed particle image velocimetry (PIV). The experiments were conducted on a scaled model of the polar icebreaker situated on a costume-built and computer-controlled turntable, which provided the ability to accurately change the incidence angle of the impinging flow with a given rate of change for incidence angle. Quantitative flow field data were obtained in several vertical and horizontal planes. The obtained velocity field was then used to calculate the time-averaged flow structure and turbulence metrics over the helicopter platform of the vessel. The present work compared the effects of two types of inflow conditions: (i) a uniform flow and (ii) a simulated atmospheric boundary layer (ABL) on the flow structure over the helicopter platform of the ship. Moreover, for the bluff scaled model, the effects of the Reynolds number on the wake structure and the flow patterns were investigated. The incidence angle (α) between the oncoming flow and the orientation of the ship varied between 0° to 330° with the increment of 30°. It was observed that higher maximum values of the turbulence intensity were associated with the simulated ABL. Moreover, it was found that for both inflow conditions, the incidence angle of 300o corresponded to the highest turbulence levels over the helicopter platform. Building on the results obtained for a stationary vessel in the simulated ABL, this work aimed to quantify the effects of the unsteady change in the direction of the impinging wind, simulated by rotating the model at a certain rate, . It was observed that the increase of the rate of change of the inflow direction resulted in an increase of the turbulent intensity over the helicopter platform. However, an exception was observed for the case of α = 60°, where clockwise rotation of the ship model with respect to the inflow exposed the helicopter platform to increased turbulent velocity fluctuations, while counterclockwise rotation diminished the flow unsteadiness over the helicopter platform. Moreover, aiming to identify the origins of the unsteady forces applied on bluff elongated plates with high chord-to thickness ratio (c/t = 23) at zero incidence, direct force measurement as well as PIV were used to identify the effect of transverse perforations on the flow-induced loading on the flow structure in the near-wake of the plates. The experiments were conducted in a water channel, where the plates were located at the center of channel, parallel to the upstream flow direction. Plates with various characteristic diameter of the perforation as well as a reference case without perforations were considered. The spectra of the trailing-edge vortex shedding and flow-induced forces were compared and it was observed that the vortex shedding frequencies were in very good agreement with those of the measured flow-induced forces for all considered perforation patterns. Thus, it was determined that the trailing-edge vortex shedding was the main mechanism of generating the unsteady loading on the plates. The staggered patterns of the perforations created a three-dimensional flow structure at the vicinity of the trailing edge and in the near wake, which was investigated using PIV at several data acquisition planes. It was found that in the cross-sectional planes corresponding to the close proximity of the perforations to the downstream edge, the periodic trailing-edge vortex shedding were suppressed. Furthermore, it was observed that for small perforations, the velocity fluctuations in the near wake were enhanced. However, further increase of the perforation diameter led to suppression of the velocity fluctuations. / Graduate

No description available.

Hydrodynamic drag testing was conducted for eleven different configurations of IsoTruss® lattice structures. Flow visualization of prototypical IsoTruss® wind towers was also performed using Particle Image Velocimetry instrumentation. The drag test and flow visualization specimens included 6-node and 8-node configurations, single and double-grid geometries, thick and thin member sizes, smooth and rough surface finishes, a helical-only structure, and a smaller outer diameter test specimen. Three sets of hydrodynamic drag tests were conducted in a closed-circuit water tunnel: 1) orientation drag tests, 2) water velocity drag tests, and 3) height variation drag tests. The orientation drag tests measured the hydrodynamic drag force of the IsoTruss® test specimens at five different orientations with an average water velocity of 1.43 mph (0.64 m/s). The water velocity drag tests measured the maximum drag for each IsoTruss® test specimen at water velocities ranging from 0.0 to an average 1.43 mph (0.64 m/s). Based on the average outer structure diameter of the IsoTruss® specimens, the water velocities corresponded to a Reynolds number range of 7,000 to 80,000. Based on the average member diameter, the corresponding Reynolds number spanned from 600 to 3,000. In addition, the height variation drag tests were performed by vertically extracting the IsoTruss® test specimens from the test section at four different immersed height levels, with a maximum immersed height of 7.22 in (18.1 cm). The height variation testing corresponded to a Froude number range of 0.40 to 0.90. The IsoTruss® specimens exhibited an average lower drag coefficient based on the projected cylindrical area than the smooth circular cylinder data throughout the Reynolds number and Froude number ranges. The drag coefficient based on solid member area showed no correlation when shown as a function of the solidity ratio. However, for the drag coefficient calculated from the solid member projected area, the data for all IsoTruss® test specimens collapsed to a 2nd order polynomial when presented as a function of the Froude number, with an R2 of 0.99. Conversely, no significant relationship was shown when the drag coefficient based on projected cylindrical area was plotted versus the Froude number. The hydrodynamic data was compared to aerodynamic data, and the orientation testing results were identical. The hydrodynamic data differed by an average of 17% compared to the non-dimensional aerodynamic results. The flow visualization research revealed that the velocity returned to 2% of the freestream velocity at 1.24 diameters upstream from the prototypical IsoTruss® wind tower. Likewise, the velocity returned to a maximum 4% of the freestream velocity at 0.94 diameters sidestream of the model IsoTruss® wind tower.

The vocal folds are an essential component of human speech production and communication. Advancements in voice research allow for improved voice disorder treatments. Since in vivo analysis of vocal fold function is limited, models have been developed to simulate vocal fold motion. In this research, synthetic and computational vocal fold models were used to investigate various aspects of vocal fold vibratory characteristics. A series of tests were performed to quantify the effect of varying material and geometric parameters on the models' flow-induced responses. First, the influence of asymmetric vocal fold stiffness on voice production was evaluated using life-sized, self-oscillating vocal fold models with idealized vocal fold geometry. Asymmetry significantly influenced glottal jet flow, glottal area, and vibration frequency. Second, flow-induced responses of simplified and MRI-based synthetic models were compared. The MRI-based models showed remarkable improvements, including less vertical motion, alternating convergent-divergent glottal profile patterns, and mucosal wave-like movement. Third, a simplified model was parametrically investigated via computational modeling techniques to determine which geometric features influenced model motion. This parametric study led to identification and ranking of key geometric parameters based on their effects on various measures of vocal fold motion (e.g., mucosal wavelike movement). Incorporation of the results of these studies into the definition of future models could lead to models with more life-like motion.