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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Diversidade morfológica e molecular e filogenia de isolados brasileiros de tripanossomas de cobras, jacarés e lagartos. / Morphological and molecular diversity and phylogeny of brazilian isolates of trypanosomes from snakes, caimans and lizards.

Viola, Laerte Bento 15 January 2008 (has links)
Tripanossomas de répteis há muito são descritos em lagartos, tartarugas, crocodilos e cobras de todos os continentes. A classificação destes tripanossomas tem sido feita de acordo com a morfologia de formas sanguíneas e hospedeiro de origem. No entanto, estes critérios tradicionais não são suficientes para descrição de espécies, gerando varias sinonímias. Vinte e sete isolados foram mantidos em culturas contínuas. Estas são as primeiras culturas de tripanossomas de lagartos da América do Sul, e as unicas culturas de tripanossomas de jacarés e cobras disponíveis no mundo. As analises filogenéticas de tripanossomas de répteis realizadas usando seqüências dos genes SSUrRNA e gGAPDH corroboram a polifília de tripanossomas de répteis. O clado que inclui isolados provenientes de cobras e lagartos sugere uma associação com hospedeiros Squamata, sugerindo que flebotomíneos tenham um papel importante na transmissão destes tripanossomas. Os dados morfológicos e moleculares resultantes deste estudo permitiram a descrição de 7 novas espécies de tripanossomas. / Reptile trypanosomes have been described in lizards, tortoises, crocodiles and snakes of all continents. Reliable classification of reptile trypanosomes requires analysis based on traditional parameters and molecular phylogeny. However, these criteria are not enough for species description, generating synonymies Twenty-seven isolates were established in continuous cultures: 9 from lizards, 8 from snakes and 10 from caimans. Cultures of these isolates displayed distinct morphology and growth behavior, representing the first cultures of lizard trypanosomes from S. America and the only available cultures from caimans and snakes. The phylogenetic analysis of the reptile trypanosome using SSU rRNA and gGAPDH gene sequences corroborated the polyphyly of reptile trypanosomes. The clade harboring snake and lizard trypanosomes suggested an association with Squamata hosts, with evidence that sand flies play an important role in the transmission of these trypanosomes. Morphological and molecular data from this study permitted the description of 7 new trypanosome species.
2

Diversidade morfológica e molecular e filogenia de isolados brasileiros de tripanossomas de cobras, jacarés e lagartos. / Morphological and molecular diversity and phylogeny of brazilian isolates of trypanosomes from snakes, caimans and lizards.

Laerte Bento Viola 15 January 2008 (has links)
Tripanossomas de répteis há muito são descritos em lagartos, tartarugas, crocodilos e cobras de todos os continentes. A classificação destes tripanossomas tem sido feita de acordo com a morfologia de formas sanguíneas e hospedeiro de origem. No entanto, estes critérios tradicionais não são suficientes para descrição de espécies, gerando varias sinonímias. Vinte e sete isolados foram mantidos em culturas contínuas. Estas são as primeiras culturas de tripanossomas de lagartos da América do Sul, e as unicas culturas de tripanossomas de jacarés e cobras disponíveis no mundo. As analises filogenéticas de tripanossomas de répteis realizadas usando seqüências dos genes SSUrRNA e gGAPDH corroboram a polifília de tripanossomas de répteis. O clado que inclui isolados provenientes de cobras e lagartos sugere uma associação com hospedeiros Squamata, sugerindo que flebotomíneos tenham um papel importante na transmissão destes tripanossomas. Os dados morfológicos e moleculares resultantes deste estudo permitiram a descrição de 7 novas espécies de tripanossomas. / Reptile trypanosomes have been described in lizards, tortoises, crocodiles and snakes of all continents. Reliable classification of reptile trypanosomes requires analysis based on traditional parameters and molecular phylogeny. However, these criteria are not enough for species description, generating synonymies Twenty-seven isolates were established in continuous cultures: 9 from lizards, 8 from snakes and 10 from caimans. Cultures of these isolates displayed distinct morphology and growth behavior, representing the first cultures of lizard trypanosomes from S. America and the only available cultures from caimans and snakes. The phylogenetic analysis of the reptile trypanosome using SSU rRNA and gGAPDH gene sequences corroborated the polyphyly of reptile trypanosomes. The clade harboring snake and lizard trypanosomes suggested an association with Squamata hosts, with evidence that sand flies play an important role in the transmission of these trypanosomes. Morphological and molecular data from this study permitted the description of 7 new trypanosome species.

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