Made available in DSpace on 2014-06-11T19:22:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-08Bitstream added on 2014-06-13T18:08:40Z : No. of bitstreams: 1 voltarelli_fa_me_rcla.pdf: 531219 bytes, checksum: 64c2defaa6f779e32ae3c17d4745efee (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O presente estudo teve por objetivo determinar o Lan pelo teste do lactato mínimo (TLM) em ratos adultos durante exercício de natação. Além disso, visou verificar se o Lan assim determinado: a) sofre influência dos estoques musculares de glicogênio; b) é sensível às alterações do condicionamento aeróbio decorrentes do treinamento físico c) é aplicável a animais portadores de transtorno metabólico e d) é sensível às alterações do metabolismo protéico muscular durante exercício de natação em intensidades inferior, equivalente e superior ao Lan. De acordo com os resultados obtidos: a) o treinamento físico aumentou a carga de trabalho correspondente ao Lan e reduziu a concentração sanguínea de lactato nessa carga, indicando que o TLM foi sensível às alterações do condicionamento aeróbio impostas pelo treinamento; b) a depleção dos estoques de glicogênio muscular pelo jejum não alterou a carga de trabalho equivalente ao Lan mas reduziu a concentração sanguínea na qual o mesmo apareceu; c) a redução da densidade corporal causada pela obesidade não mudou a carga de trabalho na qual o Lan é observado, mas diminuiu a concentração de lactato sanguíneo nessa carga, mostrando a viabilidade do TLM em animais com distúrbio metabólico; d) o TLM mostrou-se sensível às alterações do metabolismo protéico impostas pelo exercício; e) em todos os casos, houve estabilização do lactato sanguíneo durante exercício realizado na intensidade equivalente ao Lan determinado pelo TLM e f) tomados em conjunto, os resultados do presente estudo indicam a reprodutibilidade e fidedignidade do teste e sugerem a viabilidade do TLM na determinação do Lan em ratos. / The present study aimed the determination of the AT in adult rats by the lactate minimum test (LMT) and to verify: a) the effects of muscle glycogen stores on AT determined by LMT; b) the sensibility of the LMT to the alterations of the aerobic conditioning imposed by physical training; c) the applicability of the LMT to rats with altered metabolic condition and d) the effects of the swimming exercise performed at intensities below, equivalent and above AT, on the protein metabolism of rats. The LMT is based on the premise that during an incremental exercise test performed after a bout of maximal exercise, blood lactate decreases to a minimum value and then increases again. According to the results: a) physical training increased the work load corresponding to the AT and decrease the blood lactate concentration in which appeared, indicating the sensibility of the test to the alterations of the aerobic conditioning caused by training; b) the depletion of the muscle glycogen stores imposed by fasting did not modify the work load at AT, but reduced the blood lactate concentration; c) the reduced body density associated to obesity did not change the workload equivalent to AT but reduced the blood lactate concentration at this load, showing the viability of LMT to animals submitted to metabolic disturbance; d) the LMT seems to be sensitive to metabolic alterations (protein metabolism) imposed by exercise and e ) taken together these findings indicate the feasibility of the LMT test application in studies with rats.

Orientador: Eliete Luciano / Banca: Alcides Guimarães / Banca: Maria Alice Rostom de Mello / Banca: Flávio Henrique Caetano / Banca: Silvia Cristina Crepaldi Alves / Resumo: Este trabalho teve por objetivo estudar a influencia do treinamento físico aeróbio sobre o eixo de crescimento GH/IGF-1 em ratos diabéticos experimentais. Para o estudo, os ratos foram distribuídos em 4 grupos, controle sedentário (CS), controle treinado (CT), diabético sedentário (DS) e diabético treinado (DT). O diabetes experimental foi induzido por meio de Aloxana (35mg/kg). O programa de treinamento físico consistiu em natação por 60 minutos diários, 5 dias por semana, com uma carga equivalente à 5% do peso corporal, durante 6 semanas consecutivas. Na 5º semana de estudo, os ratos foram submetidos ao teste de tolerância à glicose (GTT), ao teste de tolerância à insulina (ITT) e a uma sessão de exercício agudo. Ao final do período experimental, os ratos foram sacrificados e o sangue foi utilizado para dosagem de glicose, insulina, proteínas, albumina, fosfatase alcalina, hormônio do crescimento (GH), fator de crescimento semelhante à insulina (IGF-1) e proteína transportadora de IGF-1 (IGFBP-3). Amostras do tecido hepático foram utilizadas para avaliação de glicogênio, proteínas totais e DNA, enquanto amostras do músculo gastrocnêmio foram utilizadas para avaliação de glicogênio, proteínas totais, DNA e do peptídeo IGF-1. As tíbias foram retiradas para avaliação do peso, comprimento, densidade mineral e área total. Os resultados obtidos foram avaliados pela análise de variância (ANOVA) com aplicação do teste de Bonferroni, e o nível de significância foi estabelecido em 5%. Para a avaliação das correlações entre variáveis, foi realizada a análise de Spearman's rank order. O diabetes reduziu as concentrações séricas de GH, IGF-1, IGFBP-3, além de reduzir a área total das tíbias no grupo DS... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The purpose of this study was to investigate the influence of aerobic physical training on the GH/IGF-1 growth axis in experimental diabetic rats. For the study, male Wistar rats were distributed into 4 groups, sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Experimental diabetes was induced by Aloxan (35mg/b.w.) The training program consisted in swimming 5 days/week, 1 h/day, supporting a load of 5% b.w., during 6 weeks. At the 5th week the rats were submitted to glucose (GTT) and insulin (ITT) tolerance tests, and to an acute exercise test. At the end of the experimental period, the rats were sacrificed and the blood was collected for determinations of serum glucose, insulin, proteins, albumin, alkaline phosphatase, growth hormone (GH), insulin-like growth factor (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3). Samples of liver tissue were used to evaluate glycogen, protein and DNA contents and samples of muscle (gastrocnemius) were used to evaluate glycogen, protein, DNA and IGF-1 contents. The tibias were collected for weight length, mineral density and total area determination. The results were analyzed by ANOVA, and Bonferroni test and the significance level was set at 5%. To evaluate the relationship between variables, Spearman's rank order analysis was performed. Diabetes decreased serum GH, IGF-1, IGFBP-3 and total tibia area in SD group (P<0.05). Diabetes also resulted in increase in glycaemia and serum alkaline phosphatase. Moreover, there was significant reduction in serum glucose in TD group when compared to SD. Aerobic training promoted increase in serum IGF-1 in both TC and TD groups and increased liver glycogen store... (Complete abstract, click electronic address below) / Doutor

Orientador: Maria Alice Rostom de Mello / Resumo: O presente estudo teve por objetivo determinar o Lan pelo teste do lactato mínimo (TLM) em ratos adultos durante exercício de natação. Além disso, visou verificar se o Lan assim determinado: a) sofre influência dos estoques musculares de glicogênio; b) é sensível às alterações do condicionamento aeróbio decorrentes do treinamento físico c) é aplicável a animais portadores de transtorno metabólico e d) é sensível às alterações do metabolismo protéico muscular durante exercício de natação em intensidades inferior, equivalente e superior ao Lan. De acordo com os resultados obtidos: a) o treinamento físico aumentou a carga de trabalho correspondente ao Lan e reduziu a concentração sanguínea de lactato nessa carga, indicando que o TLM foi sensível às alterações do condicionamento aeróbio impostas pelo treinamento; b) a depleção dos estoques de glicogênio muscular pelo jejum não alterou a carga de trabalho equivalente ao Lan mas reduziu a concentração sanguínea na qual o mesmo apareceu; c) a redução da densidade corporal causada pela obesidade não mudou a carga de trabalho na qual o Lan é observado, mas diminuiu a concentração de lactato sanguíneo nessa carga, mostrando a viabilidade do TLM em animais com distúrbio metabólico; d) o TLM mostrou-se sensível às alterações do metabolismo protéico impostas pelo exercício; e) em todos os casos, houve estabilização do lactato sanguíneo durante exercício realizado na intensidade equivalente ao Lan determinado pelo TLM e f) tomados em conjunto, os resultados do presente estudo indicam a reprodutibilidade e fidedignidade do teste e sugerem a viabilidade do TLM na determinação do Lan em ratos / Abstract: The present study aimed the determination of the AT in adult rats by the lactate minimum test (LMT) and to verify: a) the effects of muscle glycogen stores on AT determined by LMT; b) the sensibility of the LMT to the alterations of the aerobic conditioning imposed by physical training; c) the applicability of the LMT to rats with altered metabolic condition and d) the effects of the swimming exercise performed at intensities below, equivalent and above AT, on the protein metabolism of rats. The LMT is based on the premise that during an incremental exercise test performed after a bout of maximal exercise, blood lactate decreases to a minimum value and then increases again. According to the results: a) physical training increased the work load corresponding to the AT and decrease the blood lactate concentration in which appeared, indicating the sensibility of the test to the alterations of the aerobic conditioning caused by training; b) the depletion of the muscle glycogen stores imposed by fasting did not modify the work load at AT, but reduced the blood lactate concentration; c) the reduced body density associated to obesity did not change the workload equivalent to AT but reduced the blood lactate concentration at this load, showing the viability of LMT to animals submitted to metabolic disturbance; d) the LMT seems to be sensitive to metabolic alterations (protein metabolism) imposed by exercise and e ) taken together these findings indicate the feasibility of the LMT test application in studies with rats / Mestre

The purpose of this study was to evaluate the Virginia Military Institute (VMI) Rat Challenge program. The Rat Challenge is designed to foster self-confidence and physical conditioning in Fourth Classmen by creating training situations which are stressful enough to show them they are capable of doing tasks which surpass their previously self-imposed mental and physical limits. The program is designed to provide leadership development opportunities for the cadre. It places the responsibility on the cadets for leading and teaching the Fourth Classmen as they advance through the exercises and participate in activities which have calculated elements of risk that make safety paramount. In designing the Rat Challenge, VMI has named 10 goals and objectives of each Challenge Station. They are: improve physical condition, help conquer fears, improve relations with cadre, improve self-esteem, help build team spirit, improve ability to resolve conflict, improve sensitivity to differences in ability, develop problem solving skills, and have fun. The physical education department has not assessed these goals and objectives after completion of the program. Two research questions were asked. What level of achievement was reached by participating VMI cadets by Rat Challenge station? What differences by group(gender, class standing, VMI legacy, siblings at VMI, participation in intramural activities, participation in organized sports, prior military experience, parental prior military experience, parents presently in the military) were observed? The survey population included the Rats and cadre involved in the Rat Challenge at VMI. A two-part, paper and pencil, self-report questionnaire was utilized. Respondents were asked to respond using a Likert-type scale. An open-ended response section followed. Respondents were asked to write any additional comments regarding each Challenge Station. Significant differences in means were found in 6 out of 10 objectives and 9 out of 21 stations. / Master of Arts

No description available.

Made available in DSpace on 2014-06-11T19:30:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-03-18Bitstream added on 2014-06-13T21:01:30Z : No. of bitstreams: 1 gomes_rj_dr_rcla.pdf: 328015 bytes, checksum: 2cb5e0d5012cb02f308be4fee1dcf4e4 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Este trabalho teve por objetivo estudar a influencia do treinamento físico aeróbio sobre o eixo de crescimento GH/IGF-1 em ratos diabéticos experimentais. Para o estudo, os ratos foram distribuídos em 4 grupos, controle sedentário (CS), controle treinado (CT), diabético sedentário (DS) e diabético treinado (DT). O diabetes experimental foi induzido por meio de Aloxana (35mg/kg). O programa de treinamento físico consistiu em natação por 60 minutos diários, 5 dias por semana, com uma carga equivalente à 5% do peso corporal, durante 6 semanas consecutivas. Na 5º semana de estudo, os ratos foram submetidos ao teste de tolerância à glicose (GTT), ao teste de tolerância à insulina (ITT) e a uma sessão de exercício agudo. Ao final do período experimental, os ratos foram sacrificados e o sangue foi utilizado para dosagem de glicose, insulina, proteínas, albumina, fosfatase alcalina, hormônio do crescimento (GH), fator de crescimento semelhante à insulina (IGF-1) e proteína transportadora de IGF-1 (IGFBP-3). Amostras do tecido hepático foram utilizadas para avaliação de glicogênio, proteínas totais e DNA, enquanto amostras do músculo gastrocnêmio foram utilizadas para avaliação de glicogênio, proteínas totais, DNA e do peptídeo IGF-1. As tíbias foram retiradas para avaliação do peso, comprimento, densidade mineral e área total. Os resultados obtidos foram avaliados pela análise de variância (ANOVA) com aplicação do teste de Bonferroni, e o nível de significância foi estabelecido em 5%. Para a avaliação das correlações entre variáveis, foi realizada a análise de Spearman's rank order. O diabetes reduziu as concentrações séricas de GH, IGF-1, IGFBP-3, além de reduzir a área total das tíbias no grupo DS... / The purpose of this study was to investigate the influence of aerobic physical training on the GH/IGF-1 growth axis in experimental diabetic rats. For the study, male Wistar rats were distributed into 4 groups, sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Experimental diabetes was induced by Aloxan (35mg/b.w.) The training program consisted in swimming 5 days/week, 1 h/day, supporting a load of 5% b.w., during 6 weeks. At the 5th week the rats were submitted to glucose (GTT) and insulin (ITT) tolerance tests, and to an acute exercise test. At the end of the experimental period, the rats were sacrificed and the blood was collected for determinations of serum glucose, insulin, proteins, albumin, alkaline phosphatase, growth hormone (GH), insulin-like growth factor (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3). Samples of liver tissue were used to evaluate glycogen, protein and DNA contents and samples of muscle (gastrocnemius) were used to evaluate glycogen, protein, DNA and IGF-1 contents. The tibias were collected for weight length, mineral density and total area determination. The results were analyzed by ANOVA, and Bonferroni test and the significance level was set at 5%. To evaluate the relationship between variables, Spearman's rank order analysis was performed. Diabetes decreased serum GH, IGF-1, IGFBP-3 and total tibia area in SD group (P<0.05). Diabetes also resulted in increase in glycaemia and serum alkaline phosphatase. Moreover, there was significant reduction in serum glucose in TD group when compared to SD. Aerobic training promoted increase in serum IGF-1 in both TC and TD groups and increased liver glycogen store... (Complete abstract, click electronic address below)

Syfte och frågeställningar: Syftet med studien var att beskriva skador och träningsvanor hos löpare som uppsöker sjukgymnast på stora träningscenter i Stockholmsområdet. Frågeställningarna var: Vilka löprelaterade skador förekommer? Hur ser träningsvanor hos dessa löpare ut? Hur många har haft tidigare löprelaterade skador? Hur ser skillnaden i löpmängd ut mellan kvinnor och män samt mellan knäskadade och icke-knäskadade? Metod: En enkät utformades och lämnades ut till 18 sjukgymnaster som arbetar på 12 träningscenter i Stockholmsområdet. Frågorna i enkäten baserades på en tidigare enkät och modifierades och anpassades efter tillgänglig litteratur och studiens syfte. Enkäten var indelad i 4 delar och innehöll frågor om demografi, löpning, andra träningsvanor och skador. Personer 18 år och äldre med smärta i benet som uppkommit i samband med löpning inkluderades i studien. Totalt samlades 40 enkäter in under perioden 2014-01-29 till och med 2014-06-27. Deskriptiv statistik användes i form av antal och procent samt medelvärde och standarddeviation för normalfördelad data och median och interkvartilt avstånd (IQR) för icke-normalfördelad data. Analytisk statistik i form av student t-test för normalfördelad data och Mann Whitney-U för icke-normalfördelad data användes för de jämförande analyserna. Resultat: Av 40 deltagare var 19 st kvinnor och 21 st män. Knäskada var den mest förekommande skadan, vilket totalt 52.5 % angav. Meniskskada var den vanligaste diagnosen vilket 20 % av deltagarna hade följt av löparknä/iliotibialt smärtsyndrom (17.5 %) och achillestendinos (12.5 %). Asfalt var det vanligaste underlaget att springa på. Långdistans var den mest frekventa löpträningen följt av intervall och backträning. De flesta deltagarna styrketränade (78 %). Av de 40 deltagarna hade 23 st (57.5 %) haft en tidigare löprelaterad skada och endast 12 st blev besvärsfria ifrån denna tidigare skada. Det fanns ingen signifikant skillnad i löpmängd mellan knäskadade och icke-knäskadade. Dock fanns en tendens till signifikant skillnad mellan knäskadade och icke-knäskadade gällande löphastighet (p = 0.05). Det fanns en signifikant skillnad mellan kvinnor och män gällande löphastighet (p = 0.02), men inte gällande antal sprungna kilometer per vecka, antal minuter löpning eller antal löptillfällen per vecka. Slutsats: Studien visar att knäskada är den vanligaste skadan och meniskskada den vanligaste diagnosen följt av löparknä/iliotibialt smärtsyndrom och achillestendinos hos löpare som uppsöker sjukgymnast på de utvalda träningscentrena i Stockholmsområdet. Mer än hälften av deltagarna har haft en tidigare löprelaterad skada.  Framtida studier med fler deltagare behövs för att kunna undersöka och dra generella slutsatser om samband mellan löpmängd och olika löprelaterade skador samt om träning såsom styrketräning kan användas i skadeförebyggande syfte. / Aim: The purpose of the study was to describe injuries and training characteristics in runners who consult a physical therapist at a large training center in the County of Stockholm. Research questions: What running related injuries (RRI) do they have? What are the training characteristics of these runners? How many have had earlier RRIs? How does running volume in this sample differ between men and women as well as between knee-injured and non-knee injured runners? Method: A questionnaire was designed and handed to 18 physical therapists who work at 12 different training centers in the County of Stockholm. The questions in the questionnaire were based on a previous questionnaire then modified based on previous studies and adjusted to fit the purpose of the study. The questionnaire was divided into 4 parts with questions about demographics, running, other forms of training and injuries. People, 18 years and older, with pain from the hip down, that started during running were included in the study. A total of 40 questionnaires were collected from 2014-01-29 to 2014-06-27. Descriptive statistics were used as number of observations and percent as well as mean and standard deviation for normally distributed data and median and inter quartile range (IQR) for non-normally distributed data. Statistical analysis was performed using student t-test for normally distributed data and Mann Whitney-U test for non-normally distributed data. Results: There were a total of 40 participants, 19 female and 21 male. Knee injury was the most common injury which 52.5 % of the participants stated. Meniscal injury was the most common diagnosis with 20 % of the participants followed by runner’s knee/iliotibial band syndrome (17.5 %) and achillestendinosis (12.5 %). Asphalt was the most commonly used running surface. Long distance running was the most common type of running followed by interval and hill training. Most of the participants were performing strength training (78 %). 23 (57.5 %) of the 40 participants have had a previous running related injury. There was not a significant difference in running volume between participants with knee injury and non-knee injury. There was a tendency towards a significant difference in running speed between runners with knee injury and non- knee injury (p = 0.05). There was a significant difference between women and men regarding running speed (p = 0.02) but not regarding number of kilometers, number of minutes or number of occasions of running per week.  Conclusions: The study demonstrates that knee injury is the most common running injury and that meniscal injury is the most common diagnosis followed by runner’s knee/iliotibial band syndrome and achillestendinosis in runners diagnosed by a physical therapist at the chosen training centers in the County of Stockholm. More than 50% have had a previous running related injury. Future studies with more participants are needed in order to draw conclusions on relationships between running volume and RRIs and if strength training can be used as prevention of RRIs. / <p>Presenterat vid OMT/FYIM Kongress/Årsmöte Täby Park hotell 20-21 mars 2015</p>

Made available in DSpace on 2014-06-11T19:32:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-02-19Bitstream added on 2014-06-13T20:42:58Z : No. of bitstreams: 1 arca_ea_dr_botfm.pdf: 1044660 bytes, checksum: 0fce8dd210cd1da0f10aedd10a8511d0 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O propósito deste trabalho foi comparar os efeitos do treinamento aquático com o treinamento físico realizado no solo sobre a pressão arterial, medidas antropométricas, variáveis bioquímicas e hormonais em hipertensas. Foram avaliadas 52 mulheres hipertensas, randomizadas em três grupos: grupo água (GA; n=19), grupo solo (GS; n=19) e grupo controle (GC; n=14). Em todos os grupos foram realizadas análises da atividade da renina plasmática (APR) e do peptídeo natriurérico do tipo B (BNP) antes e após as 12 semanas de programas de exercícios. Os dados paramétricos foram expressos em média e desvio-padrão, sendo utilizada a análise de variância de duas vias para medidas repetidas. Para a variável BNP foi ajustado um modelo para medidas repetidas assumindo distribuição gamma baseado em equações de estimações generalizadas. Em ambos os testes, o nível de significância foi de 5%. No GA, houve aumento de peso corporal (de 67,6±13,4 kg para 68,4±13,8 kg) e IMC (de 27,0±5,1 Kg/m2 para 27,7±5,3 Kg/m2), diminuição da PAS (mercúrio) de 136 ± 16 mm Hg no momento zero (M0) para 124±18 mm Hg no M11 e 124±15 mm Hg no M12 e da PAS (automático) que se reduziu de 132 ± 18 mm Hg no M0 para 124 ± 14 mm Hg no M8, 124 ±16 mm Hg no M10 e 125 ± 16 mm Hg no M12. No GS, houve diminuição do VLDL (de 32,1±15,2 mg/dl para 25,8±12,5 mg/dl), da PAS (mercúrio), de 138 ± 15 mm Hg no M0 para 125 ± 10 mm Hg no M7, 127 ± 10 mm Hg no M10 e 126 ± 9 mm Hg no M12 e da PAS (automático) de 135 ±20 mm Hg no M0 para 125 ± 11mm Hg no M10. O grupo controle não teve modificações estatísticas em quaisquer das variáveis avaliadas. Os grupos não diferiram quanto às pressões basais e diferiram quanto à pressão no fim do seguimento, sendo que a pressão arterial do grupo controle foi superior à dos grupos experimentais que foram semelhantes entre si. Nenhuma outra variável... / The purpose of this study was to compare the effects of aquatic training with physical training carried out on the floor on blood pressure, anthropometric measurements, biochemical and hormonal variables in hypertension. A total of 52 hypertensive women were randomized into three groups: Water Group (WG, n = 19), Land Group (LG, n = 19) and Control Group (CG, n = 14). In all groups analysis of plasma renin activity (PRA) and peptide natriuretic type B (BNP) were performed before and after 12 weeks of exercise programs. Parametric data were expressed as mean and standard deviation. Comparison between groups and moments was performed using two-way analysis of variance for repeated measures. For the variable BNP was set a model for repeated measures assuming gamma distribution based on generalized estimation equations. In both tests, the level of significance was 5%. In WG, there was an statistically significant increase of body weight (67,6 ± 13.4 kg to 68,4 ± 13,8 kg) and BMI (27,0 ± 5,1 kg/m2 to 27,7 ± 5,3 kg / m2), a decrease in SBP (mercury) from 136 ± 16 mm Hg at the baseline (M0) to 124 ± 18 mm Hg in M11 and 124 ± 15 mm Hg in M12. Measurements obtained by automatic device was reduced from 132 ± 18 mm Hg in M0 to 124 ± 14 mm Hg in M8, 124 ± 16 mm Hg in M10 and 125 ± 16 mm Hg in M12. In LG, there was a decrease of VLDL (from 32,1 ± 15,2 mg / dl to 25,8 ± 12,5 mg / dl), BP (mercury) of 138 ± 15 mm Hg in M0 to 125 ± 10 mm Hg in M7, 127 ± 10 mm Hg in M10 and 126 ± 9 mm Hg in M12. Measurements obtained with automatic device 135 ± 20 mm Hg in M0 to 125 ± 11 mm Hg in M10. The CG had no statistical changes in any of the variables. The groups did not differ in baseline pressures and differ in terms of pressure at the end of follow-up, the pressure in the control group was higher than that of the experimental groups. The response of blood pressure were similar in both ...(Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:22:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-05-04Bitstream added on 2014-06-13T19:28:18Z : No. of bitstreams: 1 leme_jaca_me_rcla.pdf: 614425 bytes, checksum: bf0825069c6f13243a70965f900b9372 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Para investigar os efeitos do treinamento físico aeróbio nas concentrações de IGF-I em ratos diabéticos, ratos wistar foram distribuídos em quatro grupos: controle sedentário, controle treinado, diabético sedentário e diabético treinado. O diabetes foi induzido por aloxana (32 mg/kg) e o protocolo de treinamento consistiu de natação 1 hora/dia, 5 dias/semana, durante 8 semanas e suportando 5% do peso corporal. Durante o período experimental foram registrados semanalmente o peso, ingestão de água e comida. Na 7ª semana foram determinadas a glicemia e insulinemia em jejum para cálculo do índice Homa. Ao final deste período, os ratos foram sacrificados e o sangue foi coletado para determinação da glicose, insulina, albumina, triglicerídeos séricos e hematócrito. Amostras dos músculos gastrocnêmio e sóleo foram coletadas para determinação do glicogênio. Amostras do miocárdio foram utilizadas para determinar glicogênio e triglicerídeos e do fígado para determinar as concentrações de glicogênio, triglicerídeos, proteína, DNA e IGF-I. O diabetes aumentou a glicemia em jejum e em estado alimentado, triglicerídemia, glicogênio e triglicerídeos cardíacos além do DNA hepático. A doença ainda reduziu insulinemia em jejum e em estado alimentado, razão proteína/DNA hepática e concentrações de IGF-I no sangue e fígado. O protocolo de treinamento físico reduziu nos animais diabéticos a glicemia em jejum e em estado alimentado, trigliceridemia, glicogênio e triglicerídeos cardíaco além do DNA hepático. O treinamento, por outro lado, aumentou glicogênio muscular e recuperou a razão proteína/DNA hepático e IGF-I hepático e sérico nos animais diabéticos. Em conclusão, treinamento físico moderado melhora as condições metabólicas e endócrinas, particularmente no eixo GH-IGF, em ratos diabéticos. / To investigate the influence of aerobic physical training on IGF-I concentrations in diabetic rats, male wistar rats were allocated into four groups: sedentary control, trained control, sedentary diabetic and trained diabetic. Diabetes was induced by Alloxan (32 mg/kg b.w.) and training protocol consisted of swimming 1hour/day, 5 days/week, during 8 weeks, supporting 5% b.w. During the experimental period, rats weight, water and food ingestion were weekly colected. In the 7th week, blood was collected for glicose and insulin in fasting to Homa index determination. At the end of this period, rats were sacrificed and blood was collected for determinations of serum glucose, insulin, albumin, triglycerides, IGF-I and hematocrit. Gastrocnemius and soleus muscle samples were collected for glycogen determination. Samples of myocardium were used to determine glycogen and triglyceride contents and of liver to determine glycogen, triglyceride, protein, DNA and IGF-I concentrations. Diabetes increased fasting and fed state glycemia, triglyceridemia, cardiac glycogen and triglyceride younder hepatic DNA. The disease still reduced fasting and fed state insulinemia, hepatic ratio protein/DNA and IGF-I concentrations in blood and liver. Physical training protocol was able to reduce fasting and fed state serum glucose, triglyceridemia, cardiac glycogen and trygliceride and hepatic 92 DNA, to increase gastrocnemius muscle glycogen and to recover hepatic ratio protein/DNA and blood and hepatic IGF-I concentrations in diabetic animals. In conclusion, moderate physical training improved the metabolic and endocrine conditions, particulary in GH-IGF axis, in diabetic rats.

Made available in DSpace on 2014-06-11T19:22:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-07-04Bitstream added on 2014-06-13T20:10:01Z : No. of bitstreams: 1 crespilho_dm_me_rcla.pdf: 275859 bytes, checksum: 7d173afbc11a2f0b83977a420734397e (MD5) / Secretaria de Estado de Educação / Os objetivos do presente estudo foram investigar as adaptações endócrinometabólicas em ratos submetidos ao exercício físico de natação associado com a administração de testosterona durante 8 semanas. Ratos jovens Wistar foram divididos em 4 grupos: controle sedentário (CS), controle treinado (CT), testosterona sedentário (TS), testosterona treinado (TT). O protocolo de natação consistiu de 1 hora/dia, 5 dias/semanas, durante 8 semanas, suportando durante a natação uma sobrecarga de 5% do seu peso corporal. A testosterona foi administrada 3 dias/semana, na concentração de 5 mg/kg de peso corporal do animal, por via subcutânea, também por 8 semanas. Ao final da sétima semana de experimento, os ratos foram submetidos aos testes de tolerância à glicose (GTT) e teste de tolerância à insulina (ITT). Após 10 semanas os animais foram sacrificados na condição de repouso. Amostras de sangue foram coletadas para a determinação do hematócrito, glicose, insulina, proteínas totais, colesterol total e triglicérides. Os testículos, a próstata e vesícula seminal, os tecidos adiposos foram analisados em balança analítica. O tecido muscular e hepático foram usados para determinação do glicogênio e da razão proteína/DNA. As adrenais foram pesadas e usadas para determinar o conteúdo de colesterol e ácido ascórbico. A análise estatística foi feita por ANOVA e aplicação do teste de post-hoc de Newman-Keuls, onde adequado, com nível de significância pré-fixado em 5%. Os resultados indicam que os ratos treinados tiveram maior tolerância à glicose comparados com os animais sedentários durante o GTT. Nossos dados mostram que a administração crônica da testosterona mais o treinamento físico foi associada ao aumento da sensibilidade à insulina.O grupo treinado administrado com testosterona mostrou uma maior taxa de desaparecimento... / The aims of this study were to investigate the endocrine-metabolic adaptations in rats submitted to chronic swimming exercise associate to administration of testosterone during 8 weeks. Young Wistar rats were divided into four groups: sedentary control (CS), trained control (CT), sedentary testosterone (TS), trained testosterone (TT). Training protocol consisted of swimming 1h/day, 5 days/week, during 8 weeks, supporting a load of 5% body weight. Testosterone was administered 3 times a week, in concentration of 5 mg/kg body weight, during 8 weeks. After 7 weeks rats were submitted to glucose (GTT) and insulin (ITT) tolerance tests. After 8 weeks rats were sacrificed in rest. Blood samples were collected to determine hematocrit, glucose, insulin, total protein, total cholesterol and triglyceride. Hepatic and muscular tissues were used to determine glycogen and protein/DNA ratio. Adrenals were weighed and used to determine cholesterol and ascorbic acid contents. The results were analyzed by ANOVA and Newman- Keuls post-hoc test where appropriate and the significance level was 5%. The results indicate that trained rats showed higher glucose tolerance compared to sedentary animals during GTT. Our data showed that chronic exposure to testosterone plus physical training was associated with increase insulin sensitivity. The animals trained and administered with testosterone, showed higher glucose disappearance rates when compared to other groups. Testosterone administration promoted decrease in testicular mass and increase in prostate and seminal vesicle. Training promoted increase in muscle when compared to the sedentary groups, taking us to presuppose that training stimulated glycogen syntheses. The training protocol, testosterone administration or combination of two treatments, resulted in decrease of epididimal and perirrenal adipose tissues weight... (Complete abstract click electronic access below)