Freshwater inflows are essential to the health of estuaries and minimum discharge levels must be maintained in order to sustain a healthy ecosystem. Due to the predicted 50% increase in urban population growth along the Texas coastline by the year 2050, water regulators and managers are faced with the challenge of meeting human needs, while maintaining essential freshwater inflows into estuarine ecosystems. Galveston Bay is of particular concern because 10 million people currently living within its watershed. Freshwater inflows into Galveston Bay during 2006 were determined by using daily discharge data from a United States Geological Survey (USGS) sampling gauge in the Trinity River. Changes in water quality parameters, primary productivity, and phytoplankton community structure in response to freshwater inflows, were monitored monthly to determine how the phytoplankton community responded to inflow events. Freshwater inflow into Galveston Bay during 2006 was indicative of a low-inflow year, with seven large (>7,000 ft.3 sec-1) inflow events occurring throughout the year. There were significant differences in phytoplankton biomass (Fm), photosynthetic efficiency (alpha), and photosynthetic potential (yield) of the phytoplankton community, between wet (January-April and October-December) and dry (May-September) months. Significant differences in the biomass of phytoplankton groups also occurred with cyanobacteria being present in higher concentrations during the dry months and diatoms & dinoflagellates during the wet months. Low flow periods favored cyanobacteria, which lead to decreased secondary productivity, while pulsed inflow events resulted in enhanced secondary productivity by favoring diatoms and dinoflagellates. Resource Limitation Assays (RLAs) indicated that nitrogen was a potential limiting nutrient in Galveston Bay during spring/summer, with light limitation of phytoplankton communities possibly occurring near the mouth of the Trinity River. This study demonstrates the role of freshwater inflows in determining the primary productivity and community composition of the phytoplankton in Galveston Bay over an annual cycle. Inter-annual studies are needed to elucidate the impact of freshwater inflows in years with higher inflows to Galveston Bay and determine which of these impacts need to be incorporated into water management decisions to maintain a healthy ecosystem.

The contribution of phytoplankton productivity to higher trophic levels in salt marshes is not well understood. My study furthers our understanding of possible mechanisms controlling phytoplankton productivity, abundance, and community composition in salt marshes. Across three consecutive springs (2001 to 2003), I sampled the upper Nueces Delta in south Texas, a shallow, turbid, salt marsh system stressed by low freshwater inflow and wide ranging salinity (<15 to >300 ppt). Water column productivity and respiration were estimated using a light-dark bottle technique, and phytoplankton biovolume and community composition were determined using inverted light microscopy. To determine their effect on the phytoplankton community, zooplankton and bacterioplankton abundance and several physical parameters were also assessed. Meaningful relationships among the numerous variables evaluated in this study were identified using principal component analysis (PCA). Despite high turbidity, phytoplankton productivity and biovolume were substantial. Resuspension appeared to play a major role in phytoplankton dynamics, as indicated by a positive relationship between ash weight and biovolume that explained up to 46% of the variation in the PCA. Negative relationships between zooplankton grazers and pennate diatoms of optimal sizes for these grazers suggested a functional grazing food chain in this system. Salinity also may have been important in phytoplankton dynamics, whereas nutrients appeared to play a minor role. Salinity increases may have been responsible for a decoupling observed between phytoplankton and grazers during late spring. Findings suggest hypotheses for future studies focused on the role of phytoplankton in salt marshes, particularly those stressed by reduced freshwater inflow and high salinities.

Nahua reservoir is an important water resource for supplying drinking water to the Tainan area and a part of Kaohsiung in Taiwan. In recent years, Nanhua reservoir suffers eutrophication problems as many other reservoirs in Taiwan. In order to study eutrophication problems in reservoirs, a three-dimensional hydrodynamic and water quality model has been constructed using the FVCOM (Finite Volume Coastal Ocean Model) model to simulation the hydrodynamics, the nutrient dynamics and the phytoplankton growth in the Nanhua reservoir. The modeling of 3D hydrodynamics is the basic module dominating the circulation in the reservoir. The 3D eutro-dynamics are also calculated by the water quality module, which includes the dynamic variations of chlorophyll-a (Chl-a), dissolve oxygen (DO), carbon biological oxygen demand (CBOD), ammonia nitrogen (NH3-N), nitrate nitrogen (NO3-N), organic nitrogen (ON), phosphate (PO4) and organic phosphorus (OP). The model was first calibrated with the data measured in 2007 and, then, verified with the 2008 data. The model results are in reasonable agreement with the field measurements, both in the calibration and the verification phases. The water level variations are influenced by daily supply for the drinking water treatment and the inflows from the catchment and from Chiashian aqueduct during the dry season in spring. Nutrients are mainly carried into the reservoir through these routes. The residence time in the reservoir and the phytoplankton response with the nutrient loads are calculated. The model results indicated that phytoplankton growth is limited by low temperature and long residence time during the winter. The chlorophyll levels are getting higher from spring through out summer, which is due to enough sun lights and high nutrient loads carried by the catchment runoff. Surface temperatures are higher then the bottom layers causing stratification that worsen the eutrophication problems. Besides the comparisons by hydrodynamic and water quality parameters, the Carlson Trophic State Index (CTSI) has been calculated to categorize the eutrophication levels in the reservoir, which have shown good agreement with the CTSI calculated from EPA sampling data. Therefore, the model can be used as a tool for water quality management in the Nanhua reservoir.

Thesis (Ph. D.)--Massacnusetts Institute of Technology and Woods Hole Oceanographic Institution, 1977. / Includes bibliographical references (leaves 201-220).

Estimating with precision coastal marine properties such as primary production, particulate and dissolved carbon, and red tide concentrations is a challenging but important part of marine research. It benefits not only the local communities, but also provides an important input to various global biogeochemical modeling efforts. Due to the complexity of coastal environments resulting from temporal variability of tidal and riverine influences, it is useful to develop and deploy an automated sensor network that provides real-time feedback. It can be used to validate remote sensing models to retrieve in-water constituents, and provide calibration and validation for atmospheric correction of satellite sensors. For turbid waters, satellite observations in the infrared part of the spectrum can not be used to estimate atmospheric aerosol concentration because the water is not black as is found for clearer waters. This research contribution introduces a modeling effort for a turbid coastal harbor area using a semi-analytical hyperspectral remote sensing algorithm for Case 2 waters to process data from the Autonomous Marine Optical System (AMOS). Retrieved results are then compared with field sample measurements showing satisfactory closure between measurements and theory. A time series of AMOS data over a one-month time span is examined, revealing significant variations in biological activity. A sensitivity analysis of the model is performed to expose the limitations and possible improvements to AMOS measurements in the future.

The West Florida Shelf (WFS), typically characterized as being oligotrophic, is one of the most productive continental shelves in the United States. In addition to supporting a large fishing industry, the WFS also supports high biomass blooms of the toxic dinoflagellate Karenia brevis. Because of the large ecological and economic impacts these blooms have on the area, the ECOHAB: Florida program was developed to gain a better understanding of red tides and their initiation, maintenance, and dispersal. This interdisciplinary program consisted of monthly cruises from June 1998 through December 2001, with a hiatus from January through March of 2001. Hydrography, nutrients, chlorophyll a, phaeopigments, and a wide variety of other factors were measured during the cruises. In this paper chlorophyll a and phaeopigment concentration, nutrients, and hydrographic data were examined to explain the temporal and spatial distribution of chlorophyll on the shelf. Average surface chlorophyll values were 0.55 mg/m 3 with near bottom values averaging 0.85 mg/m 3. Chlorophyll was found to be highest near the estuaries of Tampa Bay and Charlotte Harbor with a decreasing gradient seaward. Near bottom chlorophyll values were generally two to fourfold greater than surface values. Midshelf stations (35- 50 m) were characterized by high near bottom chlorophyll, whereas the offshore stations (86-200 m) were characterized by a subsurface chlorophyll maximum ranging between 40 to 80 m deep. Nutrients were generally low across the shelf except for 1998 when a subsurface intrusion of nutrient rich slope water reached to the 20 m isobath. Temperatures ranged from 14.00 ° C to 31.47° C. Salinity ranged from 30.5 to 37.50 in the study area. Four blooms of Karenia brevis, lasting several months, contributed to the high chlorophyll concentrations along the inner shelf. Maximum chlorophyll concentrations of 27.10 mg/m 3 were a result of the October 1999 to March 2000 red tide. Blooms of Trichodesmium and diatoms also were contributors to patterns seen on the shelf. Maximum chlorophyll values were generally highest in the late summer and fall except for offshore values which showed little to no seasonality. Inshore of the 50 m isobath, average phaeopigments comprised from 43 to 68 percent of the measured Chl a, while offshore values were from 68 to over 100 percent. Inshore chlorophyll distributions were attributed to riverine and estuarine flux of nutrients, localized upwelling, and recycling of nutrients aided by salinity and temperature fronts. Midshelf distributions were attributed to the movement of biologically important material through the bottom Ekman layer from offshore to the inshore regions of the shelf. Offshore distributions were attributed to Loop Current upwelling and synoptic scale processes associated with seasonal meteorological forcing.

Thalassiosira oceanica, a marine centric diatom, possesses an extracellular reductase that reduces iron (Fe(III)) bound to organic complexes as part of a high-affinity Fe transport mechanism. A number of Fe(III) organic complexes are reduced, including siderophores---effective Fe chelates produced by microorganims in response to Fe stress. Reduction rates are inversely related to the relative stability constants of the oxidized and reduced Fe chelates (log Kox/Kred), and vary by a factor of 2.4 in accordance with theoretical predictions. Under Fe-limiting conditions, reduction rates increase and the ability of T. oceanica to transport Fe from siderophores is enhanced. Iron bound to the siderophore desferrioxamine B (DFB) is reduced 2 times faster than it is taken up, suggesting that the reductase is well coupled to the Fe transporter, and can provide all the inorganic Fe to account for the measured Fe uptake rates in the presence of excess DFB. The efficacy of the reductase in providing inorganic Fe for uptake and growth is ultimately dependent on the relative concentrations of excess ligands in solution and cell surface Fe transporters competing for inorganic Fe. The rates of Fe reduction and uptake are twice as fast in cells grown in NO3- compared to those grown in NH 4+, suggesting a link with cellular N metabolism and with NO3- utilization in particular. Enhanced Fe reductase activity in NO3--grown cells enables them to maintain a 1.6-fold higher cellular Fe concentration under low Fe conditions. / Experiments conducted in the subarctic Pacific, an Fe-limited oceanic region, demonstrated that even indigenous plankton (both prokaryotic and eukaryotic plankton) have the ability to acquire Fe bound to strong organic chelates. Large phytoplankton species (>3 mum) reduce Fe bound to siderophores extracellularly. Because the predominant form of dissolved Fe in the sea is bound to strong organic complexes, a reductive mechanism as described here may be a critical step in Fe acquisition by phytoplankton.

No description available.

I investigated how competitor species respond to chemical cues released from the red tide dinoflagellate Karenia brevis. K. brevis produces a mix of unstable, relatively polar, allelopathic organic molecules that are produced and released at low concentrations. The production of these compounds also varies greatly within and among strains of K. brevis. The majority of these compounds caused sublethal reductions in competitor growth. In laboratory experiments, these compounds inhibited the growth of competitors Asterionellopsis glacialis, Skeletonema grethae, Prorocentrum minimum, and Akashiwo sanguinea, although each species was susceptible to a different sub-set of K. brevis compounds. Cell physiological state and population densities were important in dictating the susceptibility of competitors to allelopathy: phytoplankton were most susceptible to K. brevis allelopathy when in earlier growth stages (rather than later stages) and in lower cell concentrations. However, these compounds have limited negative effects on natural, mixed populations of competitors from both near and offshore environments, and competitors from inshore and offshore environments appear to respond similarly to K. brevis allelopathy. In the sensitive competitor, Thalassiosira pseudonana, allelopathic compounds ultimately caused a reshuffling of cellular nitrogen pools, altered carbon storage and impaired osmotic regulation as determined using a nuclear magnetic resonance (NMR) based metabolomics approach. By characterizing the pool of primary metabolites present in the cell after exposure to K. brevis cues, we inferred which metabolic pathways may be affected by allelopathy. For instance, concentrations of betaine and the aromatic metabolite homarine were suppressed, indicating that K. brevis allelopathy may disrupt this competitor’s ability to osmoregulate. Exposure to K. brevis cues enhanced the concentrations of glutamate and the fatty acid caprylate/caprate in T. pseudonana, suggesting that protein degradation was enhanced and that energy metabolism was altered. This contrasts with the response to K. brevis allelopathy of the diatom Asterionellopsis glacialis, which was much more resistant to chemical cues produced by K. brevis, likely through as yet unidentified detoxification pathways. Overall, my dissertation research provides insight into how species-specific, antagonistic interactions among phytoplankton competitors can affect community structure through direct or indirect mechanisms, highlights the potential role of allelopathy in the maintenance of K. brevis blooms, and uses a novel tool set (i.e., metabolomics) to determine the molecular targets of K. brevis allelopathy. It further demonstrates that planktonic communities are complex and dynamic ecological systems and that interspecific interactions between phytoplankton can have unexpected, cascading impacts in marine systems.

Phosphorus and phytoplankton dynamics in freshwater are usually thought to be tightly coupled and interdependent, yet orthophosphate uptake in situ has been observed to be mediated largely by particles of bacterial size. The purpose of this thesis is to examine the general hypothesis that freshwater bacterioplankton are markedly superior competitors for phosphorus, relative to the phytoplankton. Using isolates of three species of pelagic bacteria, and two of algae, it was shown that the bacteria possess much higher affinity orthophosphate uptake kinetics than the algae. In a Monte Carlo simulation study, the accuracy and precision of these Michaelis-Menten parameter estimates were found to depend strongly upon the experimental design matrix. Bacterial superiority in uptake was also reflected in terms of growth, in chemostat competition experiments, which also showed algal and bacterial uptake of orthophosphate to be well correlated with their relative long-term phosphorus assimilation. In parallel experiments in situ, bacterioplankton were found invariably to be responsible for more than 97% of the orthophosphate uptake. In contrast, excreted organic phosphorus was utilised almost exclusively by the phytoplankton. There is little evidence as yet that excretion and reuptake of phosphorus is important in redistributing phosphorus among the plankton.