Doctorat en Sciences / info:eu-repo/semantics/nonPublished

Doctorat en Sciences / info:eu-repo/semantics/nonPublished

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Cheang Chi-chiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 126-136). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.vi / Contents --- p.viii / List of Tables --- p.xi / List of Figures --- p.xiii / Chapter CHAPTER 1: --- Introduction and Background --- p.1 / Chapter 1.1 --- Ecology and distribution of the genus Sargassum --- p.1 / Chapter 1.2 --- Classical taxonomy of the genus Sargassum and associated problems --- p.2 / Chapter 1.3 --- Recent approaches on taxonomic studies of Sargassum --- p.4 / Chapter 1.3.1 --- Morphological studies --- p.4 / Chapter 1.3.2 --- Genetic studies --- p.5 / Chapter 1.3.3 --- Integration of genetic and morphological studies --- p.7 / Chapter 1.4 --- Phylogeographic distribution and population dispersal --- p.8 / Chapter 1.5 --- Description of species --- p.11 / Chapter 1.6 --- Objectives --- p.14 / Chapter 1.7 --- Layout of the thesis --- p.15 / Chapter CHAPTER 2: --- Morphological Examination on Sargassum hemiphyllum --- p.17 / Chapter 2.1 --- Introduction --- p.17 / Chapter 2.2 --- Methodology --- p.18 / Chapter 2.2.1 --- Sampling locations and treatment of specimens --- p.18 / Chapter 2.2.2 --- Morphological measurement --- p.22 / Chapter 2.2.3 --- Data analysis and statistical tests --- p.28 / Chapter 2.3 --- Results --- p.31 / Chapter 2.3.1 --- Choosing parameters from preliminary results --- p.31 / Chapter 2.3.2 --- Analysis on the main data set --- p.40 / Chapter 2.3.2.1 --- Layout of the data set --- p.40 / Chapter 2.3.2.2 --- Spatially conservable vs. variable parameters --- p.51 / Chapter 2.3.3 --- Conservable morphological parameters- consensus of specimens --- p.58 / Chapter 2.3.4 --- Variable morphological parameters- variation among specimens based on categorical parameters --- p.58 / Chapter 2.3.5 --- Differentiation of populations based on measurable and numerical parameters --- p.63 / Chapter 2.4 --- Discussion --- p.74 / Chapter 2.4.1 --- Temporal vs. spatial variation in the morphology of Sargassum hemiphyllum --- p.74 / Chapter 2.4.2 --- Spatially conservable characters for the taxonomic identification of Sargassum hemiphyllum --- p.77 / Chapter 2.4.3 --- Variation within Sargassum hemiphyllum --- p.77 / Chapter 2.4.4 --- Variation along the biogeographical gradient --- p.79 / Chapter CHAPTER 3: --- Genetic Analysis of RbcL-S Spacer in Sargassum hemiphyllum --- p.82 / Chapter 3.1 --- Introduction --- p.82 / Chapter 3.2 --- Methodology --- p.87 / Chapter 3.2.1 --- Extraction and purification of DNA --- p.87 / Chapter 3.2.2 --- Polymerase chain reaction (PCR) and gel electrophoresis --- p.87 / Chapter 3.2.3 --- Purification of PCR product --- p.90 / Chapter 3.2.4 --- DNA sequencing --- p.90 / Chapter 3.2.5 --- RFLP study --- p.92 / Chapter 3.2.6 --- Sequence alignment and analysis --- p.92 / Chapter 3.3 --- Results --- p.93 / Chapter 3.3.1 --- Extraction and PCR amplification --- p.93 / Chapter 3.3.2 --- Pilot study --- p.97 / Chapter 3.3.3 --- RFLP study --- p.103 / Chapter 3.4 --- Discussion --- p.108 / Chapter 3.4.1 --- Suitability of RbcL-S spacer as genetic marker at population level --- p.108 / Chapter 3.4.2 --- Two clades vs. two varieties of S. hemiphyllum --- p.109 / Chapter 3.4.3 --- Reproductive barrier --- p.110 / Chapter CHAPTER 4: --- General Discussion --- p.113 / Chapter 4.1 --- Morphological and genetic data- consistence or conflict --- p.113 / Chapter 4.2 --- Latitudinal gradient of seawater temperature in the Pacific NW --- p.115 / Chapter 4.3 --- Fluctuation in seawater salinity in the Pacific NW --- p.118 / Chapter 4.4 --- Possible initiation of speciation --- p.122 / Chapter 4.5 --- Works to be done in the future --- p.123 / References --- p.126

Orientador: Denise Maria Trombert de Oliveira / Banca: Luiz Antonio de Souza / Banca: Maria das Graças Sajo / Banca: Orlando Cavalari de Paula / Banca: Suzana Bissacot Barbosa / Resumo: Myrtaceae é uma das mais relevantes famílias da flora brasileira. Foi tradicionalmente dividida em duas subfamílias, Myrtoideae, com frutos carnosos, e Leptospermoideae, com frutos secos. Esta classificação vem sendo questionada e estudos moleculares atuais vêm reorganizando a família; a classificação mais recente divide a família em Myrtoideae e Psiloxyloideae, sendo que todas as espécies nativas do Brasil encontram-se em Myrteae. Dentro de Myrteae, trabalhos clássicos reconhecem três subtribos, distintas com base na morfologia do embrião e nas características do tegumento seminal, Eugeniinae, Myrciinae e Myrtinae; no cerrado sensu stricto da região de Botucatu, há representantes destas três subtribos. Atualmente, reconhecem-se espécies cuja posição nas subtribos é indefinida, caso de Blepharocalyx salicifolius. Apesar de sua importância taxonômica, os órgãos reprodutivos de Myrtaceae ainda são pouco conhecidos e poderiam fornecer informações relevantes para a compreensão da evolução dos caracteres nos grupos infrafamiliares. Estudos envolvendo a ontogênese de tais órgãos são ainda mais raros, e estudos ontogenéticos que busquem diferenças no desenvolvimento entre estados de caráter aparentemente idênticos com origens independentes, caso dos frutos carnosos, podem fornecer novas informações, passíveis de aplicação em classificações infrafamiliares. Desta forma, sete espécies de Myrteae tiveram seus órgãos reprodutivos analisados anatomicamente, fazendo-se uso de técnicas usuais de anatomia vegetal, com relação aos seguintes aspectos: 1) Vascularização do ovário de botões florais, a fim de se definir o tipo de ovário ínfero e de se reconhecerem possíveis características utilizáveis em estudos filogenéticos; 2) Desenvolvimento dos pericarpos, procurando... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Myrtaceae is one of the most relevant families of the Brazilian flora. It was traditionally divided into two subfamilies, Myrtoideae, with fleshy fruits, and Leptospermoideae, with dry fruits. This classification has been questioned and current molecular studies have been reorganizing the family; the most recent classification has divided the family into Myrtoideae and Psiloxyloideae, and all species native to Brazil are included in Myrteae. Considering Myrteae, classic studies have recognized three subtribes that are different based on the embryo morphology and on the seed coat features, Eugeniinae, Myrciinae and Myrtinae; in sensu stricto cerrado in the region of Botucatu, there are representatives of these three subtribes. Currently, species with undefined position in the subtribes, such as Blepharocalyx salicifolius, have been recognized. Although taxonomically important, the reproductive organs of Myrtaceae are still little known and could yield relevant information to understand the evolution of traits in infrafamilial groups. Studies involving the ontogeny of such organs are even scarcer, and ontogenetic studies searching for development differences between character states apparently identical with independent origins, such as fleshy fruits, may provide new information that could be applied in intrafamilial classifications. Thus, seven Myrteae species had their reproductive organs anatomically analyzed by adopting usual techniques of plant anatomy for the following aspects: 1) Vasculature of the ovary of floral buds, to define the type of inferior ovary and to recognize characters that may be used in phylogenetic studies; 2) Pericarp development, attempting to recognize phases and/or traits that may be used in phylogenetic studies; 3) Seed development, attempting to recognize differences that... (Complete abstract click electronic access below) / Doutor

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Thèse--Faculté des Sciences de Paris. / Photocopy (positive) made in 1949 from original in Gray Herbarium, Harvard Univ.

Made available in DSpace on 2014-06-11T19:32:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-10-24Bitstream added on 2014-06-13T20:44:22Z : No. of bitstreams: 1 coneglian_irm_dr_botib.pdf: 1880517 bytes, checksum: 8f54e43516be2d23550fba138b8614d6 (MD5) / Myrtaceae é uma das mais relevantes famílias da flora brasileira. Foi tradicionalmente dividida em duas subfamílias, Myrtoideae, com frutos carnosos, e Leptospermoideae, com frutos secos. Esta classificação vem sendo questionada e estudos moleculares atuais vêm reorganizando a família; a classificação mais recente divide a família em Myrtoideae e Psiloxyloideae, sendo que todas as espécies nativas do Brasil encontram-se em Myrteae. Dentro de Myrteae, trabalhos clássicos reconhecem três subtribos, distintas com base na morfologia do embrião e nas características do tegumento seminal, Eugeniinae, Myrciinae e Myrtinae; no cerrado sensu stricto da região de Botucatu, há representantes destas três subtribos. Atualmente, reconhecem-se espécies cuja posição nas subtribos é indefinida, caso de Blepharocalyx salicifolius. Apesar de sua importância taxonômica, os órgãos reprodutivos de Myrtaceae ainda são pouco conhecidos e poderiam fornecer informações relevantes para a compreensão da evolução dos caracteres nos grupos infrafamiliares. Estudos envolvendo a ontogênese de tais órgãos são ainda mais raros, e estudos ontogenéticos que busquem diferenças no desenvolvimento entre estados de caráter aparentemente idênticos com origens independentes, caso dos frutos carnosos, podem fornecer novas informações, passíveis de aplicação em classificações infrafamiliares. Desta forma, sete espécies de Myrteae tiveram seus órgãos reprodutivos analisados anatomicamente, fazendo-se uso de técnicas usuais de anatomia vegetal, com relação aos seguintes aspectos: 1) Vascularização do ovário de botões florais, a fim de se definir o tipo de ovário ínfero e de se reconhecerem possíveis características utilizáveis em estudos filogenéticos; 2) Desenvolvimento dos pericarpos, procurando... / Myrtaceae is one of the most relevant families of the Brazilian flora. It was traditionally divided into two subfamilies, Myrtoideae, with fleshy fruits, and Leptospermoideae, with dry fruits. This classification has been questioned and current molecular studies have been reorganizing the family; the most recent classification has divided the family into Myrtoideae and Psiloxyloideae, and all species native to Brazil are included in Myrteae. Considering Myrteae, classic studies have recognized three subtribes that are different based on the embryo morphology and on the seed coat features, Eugeniinae, Myrciinae and Myrtinae; in sensu stricto cerrado in the region of Botucatu, there are representatives of these three subtribes. Currently, species with undefined position in the subtribes, such as Blepharocalyx salicifolius, have been recognized. Although taxonomically important, the reproductive organs of Myrtaceae are still little known and could yield relevant information to understand the evolution of traits in infrafamilial groups. Studies involving the ontogeny of such organs are even scarcer, and ontogenetic studies searching for development differences between character states apparently identical with independent origins, such as fleshy fruits, may provide new information that could be applied in intrafamilial classifications. Thus, seven Myrteae species had their reproductive organs anatomically analyzed by adopting usual techniques of plant anatomy for the following aspects: 1) Vasculature of the ovary of floral buds, to define the type of inferior ovary and to recognize characters that may be used in phylogenetic studies; 2) Pericarp development, attempting to recognize phases and/or traits that may be used in phylogenetic studies; 3) Seed development, attempting to recognize differences that... (Complete abstract click electronic access below)

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