Bibliography : leaves 159-184. / xv, 191 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This study found at least three mechanisms of resistance to the acetyl coenzyme A carboxylase (ACCase)-inhibiting herbicides. A modified target -site was responsible for moderate and high resistance to herbicides at the whole plant level. Enhanced herbicide metabolism and reduced translocation of herbicide to the target site was observed in one resistant biotype each. / Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1996

Bibliography: leaves 147-163. / ix, 164 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The aim of this thesis is to confirm the resistance status of three purported resistant weed species by herbicide screening of outdoor pot grown plants. Field experiments are set up to investigate changes in dormancy and seedbank life of the resistant populations over a three year period. After determining the herbicide resistance spectrum of the resistant biotypes, genetic crosses between resistant and susceptible plants are performed to follow the mode of inheritance of acetolactate synthase (ALS) resistance. "In vitro" ALS enzyme assays in the presence of various herbicides are carried out to reveal a modified ALS enzyme as the main mechanism of resistance in all cases. A molecular investigation of the ALS gene is performed to identify mutations responsible for endowing a resistant enzyme. / Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1996

Bibliography: leaves 209-254. / xv, 254 leaves : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Experiments detailed in this thesis, evaluate the impact of various herbicides and herbicide mixtures on the growth, nodulation and nitrogen fixation of annual pasture legumes, the efficacy of the herbicides for weed control, and the potential role of mechanical defoliation to replace herbicides, leading to lower cost and more sustainable farming systems. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1996

Bibliography: leaves 147-163. / ix, 164 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The aim of this thesis is to confirm the resistance status of three purported resistant weed species by herbicide screening of outdoor pot grown plants. Field experiments are set up to investigate changes in dormancy and seedbank life of the resistant populations over a three year period. After determining the herbicide resistance spectrum of the resistant biotypes, genetic crosses between resistant and susceptible plants are performed to follow the mode of inheritance of acetolactate synthase (ALS) resistance. "In vitro" ALS enzyme assays in the presence of various herbicides are carried out to reveal a modified ALS enzyme as the main mechanism of resistance in all cases. A molecular investigation of the ALS gene is performed to identify mutations responsible for endowing a resistant enzyme. / Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1996

Orientador: Caio Antonio Carbonari / Banca: Edivaldo Domingues Velini / Banca: Leandro Tropaldi / Resumo: Muito já foi e ainda vem sendo estudado sobre os efeitos clássicos de dose-resposta dos herbicidas e uma outra vertente que vem surgindo são as respostas bifásicas, que incluem hormesis, caracterizada por doses baixas de um herbicida que causa estímulo e inibição em dose elevada. Assim como o estímulo pode ser benéfico na agricultura em algumas aplicações, porem quando ocorre em plantas não alvo, o efeito de hormesis pode-se tornar um problema. Nesse trabalho foram realizados quatro experimentos. Um experimento de curva dose-resposta para biótipo resistente e suscetível de Digitaria insularis, resistente ao herbicida glyphosate, avaliando-se altura das plantas, massa fresca e seca e fitotoxicidade (%) aos 7, 14 e 21 dias após a aplicação. Houve também a repetição do mesmo, um experimento com subdosagem de glyphosate para averiguar o efeito das subdosagens no florescimento, avaliando-se o número de panículas emitidos após a aplicação dos tratamentos e o quarto experimento que foi a verificação da germinação, índice de velocidade de germinação e peso de sementes provindas do terceiro experimento. O efeito de estímulo das subdoses foram constatados em todos os experimentos sendo que diferentes faixas de doses causaram o efeito de hormesis nos biótipos de D.insularis, resistente e suscetível havendo aumento de altura, massa, peso de sementes e velocidade de germinação e diminuição do tempo necessário para emissão das inflorescências. Esses efeitos podem conceder uma grande vantag... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Currently much has been studied about the classic dose-response effects of herbicides and another aspect that is emerging are the biphasic responses, which include hormesis, a biphasic dose-response phenomenon characterized by low dose stimulation and high dose inhibition. Just as the stimulus may be beneficial in agriculture in several applications, when this stimulus occurs in non-target plants, the effect of hormesis can become a problem. In this work, a dose-response curve experiment was performed for resistant and susceptible biotypes of Digitaria insularis, resistant to the glyphosate herbicide and evaluating plant height, fresh and dry mass and phytotoxicity (%) at 7, 14 and 21 days after application. The second experiment was the repetition of the firs. An experiment with subdoses of glyphosate are performed to investigate the effect of the subdoses on flowering, evaluating the number of panicles emitted after the application of the treatments and the fourth experiment that was the verification of the germination, germination speed index and seed weight from the third experiment. The effect of subdoses stimulation was observed in all the experiments, with different dose ranges causing the stimulus in the D.insularis biotypes, resistant and susceptible, increasing height, mass, seed weight and germination speed and reduced the time required for inflorescence emission. These effects can give a great advantage to the spread of this species, helping to change the flora of weeds / Mestre

Orientador: Cláudio Cavariani / Banca: Edvaldo Aparecido Amaral da Silva / Banca: Silvio José Bicudo / Banca: Ricardo Augusto Dias Kanthack / Banca: Mariana Zampar Toledo / Resumo: A máxima qualidade das sementes de soja é expressa na maturidade fisiológica, mas, a colheita nesse estádio fenológico não é recomendada, devido ao elevado teor de água nas sementes e o excesso de folhas e hastes verdes nas plantas. Com o intuito de antecipar a colheita, a prática da dessecação química, com o herbicida glyphosate, vem sendo estudada em soja e requer alguns procedimentos, visando a preservação da qualidade fisiológica das sementes no campo. Desse modo, o trabalho teve como objetivo avaliar a qualidade fisiológica de sementes de soja de cultivares convencionais e transgênicas, oriundas de plantas dessecadas com herbicida glyphosate, em diversas épocas de dessecação. A dessecação química com o herbicida glyphosate (2,0 L.ha-1) foi realizada quando os estádios fenológicos R6.0, R7.1, R7.2 e R7.3 das plantas das cultivares BRS 133, Conquista, BRS 243RR e BRS 246RR foram atingidos. As determinações e testes para avaliação da qualidade das sementes foram: teor de água, massa de 100 sementes, germinação, primeira contagem de germinação, envelhecimento acelerado, emergência de plântulas no campo, índice de velocidade de emergência de plântulas no campo, condutividade elétrica, comprimento de plântulas e massa de matéria seca de plântulas. O delineamento experimental adotado foi o de blocos casualizados, dispostos em esquema de parcelas subdivididas, com quatro repetições, totalizando 20 tratamentos. As parcelas constaram de quatro cultivares (BRS 133, Conquista, BRS 243RR e BRS 246RR) e as subparcelas corresponderam a quatro épocas de dessecação, que corresponderam a quatro estádios da cultura (R6.0, R7.1, R7.2 e R7.3) e uma testemunha para cada cultivar (sem dessecação). Os tratamentos foram dispostos em esquema fatorial com 4 cultivares x 4 épocas de dessecação, mais as testemunhas e as médias foram comparadas pelo teste de ... / Abstract: The maximum quality of soybean seeds is expressed in physiological maturity, but, harvest is not recommended in this phase, due to the high water content in seeds and excess leaves and green stems on the plants. With the aim of anticipating the harvest, the practice of chemical desiccation, with the herbicide glyphosate, has been studied in soybean and requires some procedures, aiming at the preservation of the physiological quality of seeds in the field. In this way, the work aimed to evaluate the physiological quality of soybean seeds of conventional and transgenic cultivars from desiccated plants with herbicide glyphosate, in various periods of desiccation. Chemical desiccation with the herbicide glyphosate (2,0L.ha-1) was performed when the phenological stages R6.0, R7.1, R7.2 and R7.3 plants of cultivars BRS 133, Conquista, BRS 243RR and BRS 246RR were achieved. Tests for evaluation of the quality of the seeds were: water content, mass of 100 seeds, germination, first count of germination, accelerated aging, emergence of seedlings in the field, speed index of seedling emergence in the field, electrical conductivity, seedling length and mass of the dry matter of seedlings. The experimental design was randomized block, arranged in plots scheme subdivided, with four repetitions, totaling 20 treatments. The parcels consisted of four cultivars (BRS 133, Conquista, BRS 243RR and BRS 246RR) and the subplots corresponded to four periods of desiccation, which corresponded to four stages of culture (R6.0, R7.1, R7.2 and R7.3) and a witness to every cultivar (without desiccation). Treatments were arranged in factorial scheme 4 cultivars x 4 times of desiccation, more witnesses and the averages were compared by t test the probability 5. The application of glyphosate in phenological stages R6.0 and R7.1 of soybean plants, conventional cultivars BRS 133 and Conquista, negatively influences the physiological ... / Doutor

Orientador: Edivaldo Domingues Velini / Coorientador: Stephen O.Duke / Coorientador: Franck E.Dayan / Banca: Caio Antonio Carbonari / Banca: Antonio Luiz Cerdeira / Banca: Marcelo de Almeda Silva / Banca: Sidnei Douglas Cavalieri / Resumo: O efeito benéfico de subdoses de substâncias tóxicas é conhecido há muito tempo e este fenômeno é chamado de hormesis. Em agricultura, muitos são os casos de herbicidas que em doses normais causam severas injurias nas plantas, mas que em doses muito baixas promovem o crescimento de estruturas das plantas ou promovem outros efeitos benéficos, como aumento do teor de proteína em plantas. Objetivou-se com este trabalho avaliar o efeito de hormesis dos herbicidas glyphosate, amicarbazone, mesotrione, imazapic e carfentrazone em soja, além de explorar se o efeito de hormesis na soja é afetado por um pré-tratamento com baixas doses de glyphosate e mais tarde exposta as mesmas doses ou doses maiores. Para isso, três experimentos distintos foram conduzidos, sendo que no primeiro, sete doses do herbicida glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a.ha-1 ) foram aplicadas sobre as plantas de soja e avaliadas quanto à taxa de transporte de elétrons (ETR), acúmulo de compostos químicos alterados em função da ação do glyphosate (ácido chiquímico, ácido benzoico, ácido salicílico, AMPA, fenilalanina, tirosina e triptofano) e massa de matéria seca das plantas. No segundo experimento, foi feito um pré-tratamento das plantas com subdoses do glyphosate (0; 1,8; 3,6 e 7,2 g e.a. ha-1 ) no estádio V2 e 14 dias após esta aplicação, as plantas receberam uma segunda aplicação de diferentes doses do glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a. ha-1 ) e posteriormente avaliadas quanto ao acúmulo de massa de matéria seca e ácido chiquímico. No terceiro experimento, foram testadas uma completa curva de dose-resposta dos herbicidas amicarbazone, ... / Abstract: The beneficial effect of low doses of toxic substances is known a long time and this phenomenon is called hormesis. In agriculture, there are many cases of herbicides that in normal rates cause severe injuries in plants, but in very low doses promote the growth of plant structures or other beneficial effect such as increase in the protein content in plants. The objective of this study was to evaluate the effect of hormesis of glyphosate, amicarbazone, mesotrione, imazapic and carfentrazone in soybean, in addition to exploring if the effect of hormesis in soybeans is affected by a pretreatment with low doses of glyphosate and later expose to the same doses or higher. For this, three separate experiments were carried out, and on the first one seven doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 and 720 g a.e.ha-1 ) were applied to the soybean plants and evaluated the electron transport rate (ETR), the chemical compounds resulting from the action of glyphosate (shikimic acid, benzoic acid, salicylic acid, AMPA , phenylalanine, tyrosine and tryptophan) and hormesis effect on the dry weight of plants. The second experiment was made a pretreatment of soybean plants with low doses of glyphosate (0; 1.8; 3.6 and 7.2 g a.e.ha-1) in the V2 stage and 14 days later the plants received a second application of different doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 e 720 g a.e.ha-1 ) and subsequently evaluated the dry weight of plants and shikimic acid. In the third experiment, was tested a full dose-response of the herbicides amicarbazone, mesotrione, imazapic and carfentrazone on soybean plants and evaluated the ETR and dry weight. The results indicated that at low doses glyphosate stimulated ETR and increased biomass. The compounds from shikimic acid pathway such as benzoic and salicylic acids had respective increases of 203 and 170% in the dose of 3.6 g a.e. ha-1of glyphosate and the amino ... / Doutor

Made available in DSpace on 2015-12-10T14:22:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-17. Added 1 bitstream(s) on 2015-12-10T14:28:58Z : No. of bitstreams: 1 000853172.pdf: 668118 bytes, checksum: 6bb014fda576f413445f741abae4b6a9 (MD5) / O eucalipto é a espécie florestal mais utilizada em reflorestamento no país, em virtude do rápido crescimento das plantas, condições climáticas e tecnologias desenvolvidas. Porém, a interferência das plantas daninhas causa grandes prejuízos, decréscimo quantitativo e qualitativo da produção do eucalipto. Entre as possibilidades de manejo de plantas daninhas, o método químico é o mais utilizado, porém, poucos são os herbicidas registrados para a cultura do eucalipto, entre os quais o glyphosate é o mais usado por controlar um grande número de espécies. Existem poucos trabalhos a respeito dos efeitos diretos e indiretos do glyphosate nesta espécie florestal. Neste trabalho, clones de eucalipto urograndis foram submetidos ao contato direto com o glyphosate e indireto por meio de aplicação sobre plantas de Brachiaria decumbens, visando caracterizar o movimento do herbicida, exsudação radicular e seus efeitos na planta. Foram realizadas avaliações de intoxicação visual do eucalipto aos 7, 14 e 21 DAA e da massa da matéria seca das plantas aos 21 DAA. Também foram realizadas quantificações por cromatografia líquida de alta eficiência e espectrometria de massas (LC-MS/MS) em diferentes partes das plantas (superior e inferior) dos seguintes compostos: glyphosate, ácido amimetilfofônico (AMPA), ácido chiquímico, chiquimato-3-fosfato, fenilalanina, tirosina e triptofano. O glyphosate foi detectado nas plantas de eucalipto para os tratamentos com a aplicação direta e indireta do herbicida e houve translocação para os locais que não foram atingidos na aplicação, mesmo quando aplicado sobre a B. decumbens, entretanto o AMPA não foi. A presença de glyphosate nas plantas influenciou na alteração dos níveis de ácido chiquímico, fenilalanina, tirosina, triptofano e chiquimato-3-fosfato, além de causar níveis de intoxicação nas plantas de eucalipto e redução de ... / Eucalyptus is the most used forest species in reforestation in the country due to the rapid growth of plants, climatic conditions and developed technologies. However, interference by the weeds cause major damage, the quantitative and qualitative decrease in production of eucalyptus. Among the weed management possibilities, the chemical method is the most used, however, there are few herbicides registered for eucalyptus cultivation, in which glyphosate is most commonly used to control a large number of species. There are few studies regarding the direct and indirect effects of the glyphosate on this forest species. In this paper, Eucalyptus urograndis clones were subjected to direct contact with glyphosate and indirectly through application on Brachiaria decumbens plants, aiming to characterize the movement of the herbicide, root exudation and its effects on the plant. Visual evaluations were carried out through eucalyptus phytointoxication at 7, 14 and 21 DAA and of the dry mass of the plants at 21 DAA. Also, measurements were made on different parts of plants (top and bottom) of the following compounds by high-performance liquid chromatography and mass spectrometry (LC-MS / MS): glyphosate, aminomethylphosphonic acid (AMPA), shikimic acid, shikimate-3-phosphate, phenylalanine, tyrosine and tryptophan. Glyphosate was detected in eucalyptus plants for treatments with the direct and indirect application of herbicide and there was a translocation to sites that were not attain in the application, even when applied to the B. decumbens, however the AMPA was not. The presence of glyphosate on plants, influenced the change in the shikimic acid levels, phenylalanine, tyrosine, tryptophan, and shikimate- 3-phosphate, apart from causing intoxication in eucalyptus plants and reduction of dry mass. Glyphosate was detected in eucalyptus plants after application on the plants of B decumbens with low intoxicication ...

Made available in DSpace on 2014-12-02T11:16:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-07-07Bitstream added on 2014-12-02T11:21:41Z : No. of bitstreams: 1 000800283.pdf: 3261975 bytes, checksum: bdfd9509a74fed5a5ddfcc147954a0fb (MD5) / As cultivares de algodão Liberty Link ® são aquelas que resistem à aplicação do amônio glufosinate a uma dose que normalmente seria recomendada para os demais biótipos da espécie. Estas cultivares de algodão são constituídas geneticamente com um gene que codifica a produção da enzima fosfinotric ina acetil transferase (PAT), responsável pela acetilação do amônio glufosinate, inativando - o na planta. A cultivar de algodão FiberMax ® 975 WideStrike por também apresentar em seu DNA o gene PAT apresentaria o comportamento de seu metabolismo intermediário às cultivares convencional e Liberty Link ® . O objetivo do trabalho foi avaliar as alterações metabólicas e a intoxicação das plantas de algodão Wide Strike ® comparadas a plantas Liberty Link ® e convencionais, quando submetidos ao herbicida amônio glufosinate. Os es tudos foram conduzidos em casa de vegetação n a Faculdade de Ciências Agronômicas - FCA / UNESP - Campus de Botucatu (SP). Dois experimentos foram instalad os simultaneamente utilizand o cultivares as cultivares transgênicas e convencional , sendo o primeiro para avaliar a intoxicação e alterações na fotossíntese e o segundo para quantificar compostos relacionados a rota de ação do herbicida amônio glufosinate . Foram semeadas 5 sementes p or vaso de três cultivares de algodão FM 993 (convencional), FM 975WS ® (transgênica) e IMACD 6001LL ® (transgênica) e, aos 15 dias após a emergência (DAE) foi realizado o desbaste das plantas, mantendo duas plantas por vaso. Quando as plantas apresentaram 2 folhas verdadeiras (25 dias após emergência) realizou - se a primeira aplicação dos ... / The Liberty Link cotton cultivars are those that resist a dose of ammonium glufosinate application that normally would be recommended for other biotypes species .These cotton plants are constitute genetically with gene that encodes the production of enzyme phosphinothricin acetyl transferase (PAT) responsible for the acetylation of glufosinate ammonium, inactivating it in the plant. The cotton cultivar FiberMax® 975 WideStrike has present in i ts DNA the gene PAT and present intermediate metabolism to conve ntional cultivars and Liberty Link ®. The purpose of the study was to evaluate the metabolic changes and the intoxication of WideStrike®cotton plants compared with Liberty Link ® and conventional plants, when treated to ammonium glufosinate herbicide. The studies were carried out in a greenhouse at the Faculty of Agricultural Sciences, “Júlio de MesquitaFilho State University – Botucatu Campus (SP). Were conducted two experiments simultaneously using the transgenic and conventional cultivars, the first one to evaluate the toxicity and changes in photosynthesis and the secondto quantify compounds related to the herbicide ammonium glufosinate action route. Five seeds were sown of each cultivar per pot of the three cotton cultivars FM 993 (non - genetically modi fied), FM 975WS® (genetically modified) and IMACD 6001LL® (genetically modified) and at 15 days after emergence (DAE) was performed thinning of the plants, keeping two plants per pot. When the plants had two true leaves (25 days after emergence) held the ...

Made available in DSpace on 2014-12-02T11:16:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-05-23Bitstream added on 2014-12-02T11:21:40Z : No. of bitstreams: 1 000791298.pdf: 1255629 bytes, checksum: 167ceaa2fd11583895b9a2aee9dab019 (MD5) / O efeito benéfico de subdoses de substâncias tóxicas é conhecido há muito tempo e este fenômeno é chamado de hormesis. Em agricultura, muitos são os casos de herbicidas que em doses normais causam severas injurias nas plantas, mas que em doses muito baixas promovem o crescimento de estruturas das plantas ou promovem outros efeitos benéficos, como aumento do teor de proteína em plantas. Objetivou-se com este trabalho avaliar o efeito de hormesis dos herbicidas glyphosate, amicarbazone, mesotrione, imazapic e carfentrazone em soja, além de explorar se o efeito de hormesis na soja é afetado por um pré-tratamento com baixas doses de glyphosate e mais tarde exposta as mesmas doses ou doses maiores. Para isso, três experimentos distintos foram conduzidos, sendo que no primeiro, sete doses do herbicida glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a.ha-1 ) foram aplicadas sobre as plantas de soja e avaliadas quanto à taxa de transporte de elétrons (ETR), acúmulo de compostos químicos alterados em função da ação do glyphosate (ácido chiquímico, ácido benzoico, ácido salicílico, AMPA, fenilalanina, tirosina e triptofano) e massa de matéria seca das plantas. No segundo experimento, foi feito um pré-tratamento das plantas com subdoses do glyphosate (0; 1,8; 3,6 e 7,2 g e.a. ha-1 ) no estádio V2 e 14 dias após esta aplicação, as plantas receberam uma segunda aplicação de diferentes doses do glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a. ha-1 ) e posteriormente avaliadas quanto ao acúmulo de massa de matéria seca e ácido chiquímico. No terceiro experimento, foram testadas uma completa curva de dose-resposta dos herbicidas amicarbazone, ... / The beneficial effect of low doses of toxic substances is known a long time and this phenomenon is called hormesis. In agriculture, there are many cases of herbicides that in normal rates cause severe injuries in plants, but in very low doses promote the growth of plant structures or other beneficial effect such as increase in the protein content in plants. The objective of this study was to evaluate the effect of hormesis of glyphosate, amicarbazone, mesotrione, imazapic and carfentrazone in soybean, in addition to exploring if the effect of hormesis in soybeans is affected by a pretreatment with low doses of glyphosate and later expose to the same doses or higher. For this, three separate experiments were carried out, and on the first one seven doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 and 720 g a.e.ha-1 ) were applied to the soybean plants and evaluated the electron transport rate (ETR), the chemical compounds resulting from the action of glyphosate (shikimic acid, benzoic acid, salicylic acid, AMPA , phenylalanine, tyrosine and tryptophan) and hormesis effect on the dry weight of plants. The second experiment was made a pretreatment of soybean plants with low doses of glyphosate (0; 1.8; 3.6 and 7.2 g a.e.ha-1) in the V2 stage and 14 days later the plants received a second application of different doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 e 720 g a.e.ha-1 ) and subsequently evaluated the dry weight of plants and shikimic acid. In the third experiment, was tested a full dose-response of the herbicides amicarbazone, mesotrione, imazapic and carfentrazone on soybean plants and evaluated the ETR and dry weight. The results indicated that at low doses glyphosate stimulated ETR and increased biomass. The compounds from shikimic acid pathway such as benzoic and salicylic acids had respective increases of 203 and 170% in the dose of 3.6 g a.e. ha-1of glyphosate and the amino ...