Accurate single-shot visualization of laser wakefield structures can improve our fundamental understanding of plasma-based accelerators. Previously, frequency domain holography (FDH) was used to visualize weakly nonlinear sinusoidal wakes in plasmas of density n[subscript e] < 0.6 × 10¹⁹/cm³ that produced few or no relativistic electrons. Here, I address the more challenging task of visualizing highly nonlinear wakes in plasmas of density n[subscript e] ~ 1 to 3× 10¹⁹/cm³ that can produce high-quality relativistic electron beams. Nonlinear wakes were driven by 30 TW, 30 fs, 800 nm pump pulses. When bubbles formed, part of a 400 nm, co-propagating, overlapping probe pulse became trapped inside them, creating a light packet of plasma wavelength dimensions--that is, an optical "bullet"--that I reconstruct by FDH methods. As ne increased, the bullets first appeared at 0.8 × 10¹⁹/cm³, the first observation of bubble formation below the electron capture threshold. WAKE simulations confirmed bubble formation without electron capture and the trapping of optical bullets at this density. At n[subscript] >1× 10¹⁹/cm³, bullets appeared with high shot-to-shot stability together with quasi-monoenergetic relativistic electrons. I also directly observed the temporal walk-off of the optical bullet from the beam-loaded plasma bubble revealed by FDH phase shift data, providing unprecedented visualization of the electron injection and beam loading processes. There are five chapters in this thesis. Chapter 1 introduces general laser plasma- based accelerators (LPA). Chapter 2 discusses the FDH imaging technique, including the setup and reconstruction process. In 2006, Dr. N. H. Matlis used FDH to image a linear plasma wakefield. His work is also presented in Chapter 2 but with new analyses. Chapter 3, the main part of the thesis, discusses the visualization of LPAs in the bubble regime. Chapter 4 presents the concept of frequency domain tomography. Chapter 5 suggests future directions for research in FDH. / text

Ultra-high dose rate proton radiation has the potential to improve cancer treatment by reducing the normal tissue complication probability and, at the same time, reaching the tumor control probability known from conventional photon radiation therapy. Here, the ultra-high dose rate leads to normal tissue sparing via the FLASH effect. Before a clinical implementation is possible, the proton FLASH effect requires translational research via in-vivo irradiation studies with small animals. Laser plasma-based accelerators (LPAs) for protons offer unique opportunities for studying the proton FLASH effect, since the proton dose rate at LAPs is in the order of 10^9 Gy/s, which is unreached at conventional medical proton accelerators. Different to medical proton accelerators, LPAs are operated in a pulsed mode and feature a lower beam stability caused by inherent pulse-to-pulse fluctuations of the acceleration process. To ensure successful in-vivo irradiation studies, advanced beam delivery, monitoring and dosimetry concepts for an online-monitored application of the 3D dose distribution in the target volume (TV) of the in-vivo sample are needed. The detectors and dosimetric concept developed in this thesis enable the world wide frst pilot radiobiological in-vivo study with LPA protons, where mouse ear tumors are irradiated with ultra-high dose rate proton pulses. For performing the radiobiological study, the ALBUS-2S (Advanced Laser-driven Beamlines for User-specifc Studies - 2 Solenoids) beamline is used, which is installed at the compact petawatt (PW) laser system DRACO (Dresden laser acceleration source) at HZDR (Helmholtz-Zentrum Dresden-Rossendorf). In this thesis, a scintillator-based time-of-fight (ToF) beam monitoring sytem (BMS) is developed, which records single-pulse proton energy spectra in transmission at the ALBUS-2S beamline. A relative energy uncertainty of 5.5 % (1σ) is reached for the ToF BMS, allowing for a Monte Carlo simulation-based prediction of depth dose profiles at the irradiation site. The ToF BMS is used for characterization of the ALBUS-2S LPA beamline for application-oriented parameters, in order to qualify the LPA proton source for radiobiological in-vivo studies. Furthermore, a dosimetry and beam monitoring concept for in-vivo irradiations of small target volumes with LPA protons is presented in this thesis. With the overall relative dose uncertainty of 7.4 % (2σ) for the specifc mouse ear tumor irradiation scenario, the concept enables verifcation of accurate volumetric dose delivery to the mm-scale TVs. In addition, tomography-based approaches with scintillators are investigated as detectors for online 3D dose measurement at LPAs. The miniature scintillator dosimeter (miniSCIDOM) detector, which is developed in the scope of this thesis, is used for online 3D dose measurements in 1 cm^3 volumes with < 1 mm^3 resolution at the irradiation site of the ALBUS-2S beamline. For online 3D dose measurements directly behind the LPA proton source of the DRACO PW laser system, the optical cone beam tomograph for proton online dosimetry (OCTOPOD) detector is developed. The OCTOPOD detector has a sensitive volume of 5 cm-diameter and water equivalent thickness of 4.3 cm, which is sufficient to stop 70 MeV protons. It is designed to reach a spatial resolution of 1 mm^3. The detectors developed in this thesis are optimized tools for source-to-sample characterization of LPA beamlines and hence are an essential contribution for radiobiological in-vivo studies with LPA protons.