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DETERMINATION OF THE EFFICACY OF THE ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) IN CHARACTERIZING CROTALUS SNAKE VENOM AT THE SPECIES LEVELHitt, John Michael, 1952- January 1986 (has links)
No description available.
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The cytolytic effect of cobra cardiotoxin on ehrlich ascites tumor cells and its inhabition by calcium ions.January 1975 (has links)
Thesis (M. Ph.)--Chinese University of Hong Kong. / Bibliography: l. 73-78.
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A homoeopathic drug proving of the venom of the eastern green mamba (Dendroaspis angusticeps) analysing symptomatology in relation to the doctrine of signatures and toxicological dataHansjee, Sharad January 2010 (has links)
Dissertation submitted in partial compliance with the requirements for the Master’s Degree in Technology: Homoeopathy, Durban University of Technology, 2010. / Provings are an essential element in the development of the homoeopathic
medicine knowledge base and scope of practice. There are many South
African indigenous plants and animals that can play a role in homoeopathy
but need to be proved first. Several indigenous snake venoms have already
been proved as homoeopathic remedies, so the proving of Dendroaspis
angusticeps venom expands this particular knowledge base.
Aim
The aim of this study was to determine the effect of a homoeopathic dose of
the venom of the Dendroaspis angusticeps on healthy individuals in order to
elicit and document the resulting mental and physical symptomotology, so
that it may be prescribed to those that require it based on the Law of
Similars. The other aims of this study were to compare the proving symptoms
of Dendroaspis angusticeps 30CH to the toxicology of Eastern Green Mamba
venom and to analyze the remedy picture in relation to the doctrine of
signatures.
The thirtieth centesimal potency of the remedy was hypothesised to produce
clearly observable signs and symptoms in provers (healthy volunteers).
The symptoms obtained from the proving were also compared to the doctrine
of signature analysis of Dendroaspis angusticeps to help clarify the nature of
this snake and to expand the remedy picture of the substance and facilitate
our understanding of the remedy in light of the toxicology of the venom and
the doctrine of signatures.
vii
Methodology
The homoeopathic proving of Dendroaspis angusticeps 30CH was carried
out as a randomised, double blind, placebo-controlled trial. The proving
population consisted of 30 provers. Twenty four of the provers were in the
experimental group and they received the potentised snake venom. Six
provers were in the control group and they received the placebo. As this was
a double blind, placebo-controlled trial, neither the individuals taking part in
the study nor the researcher had any knowledge of who received placebo or
the active remedy. Provers were also unaware of the substance being proven
or the potency in which it was prepared.
Results
Provers recorded their symptoms in a journal over a period of 5 weeks and
were monitored regularly by the researcher. At the completion of the proving
period, the researcher collected all the recorded data and each journal was
assessed by the researcher to determine the suitability of the recorded
symptoms. The symptoms were then translated into the language of the
materia medica and the repertory and a remedy picture was formulated.
Thereafter a comparison was made to the nature of the snake by means of
the doctrine of signatures and the toxicology of the venom as an attempt to
broaden the repertoire of our knowledge of the action of the remedy.
Provers experienced a wide range of symptoms particularly on the mental
and emotional spheres including feelings of powerful assertion and
confidence; cheerful and excited energy; spiritual and prayerful feelings;
seductive, sensual and extravagant mannerisms; desire to dance and
heightened energy; clairvoyant natures and desires to be in nature as well as
pronounced irritability, anger and sadness. Other symptoms included desire
to be alone and withdrawn feelings; quarrelsome natures; desire to kill;
deceitful ways; intolerance to injustice; poor concentration; thoughts of the
past; confusion and antagonism of the will and anxious thoughts. Delusions,
fears and thoughts of death also manifested in provers. There was a marked
polarity of symptoms as:
Anger, rage, irritability vs calmness / spirituality;
Sadness / depression vs cheerfulness / joy;
Poor concentration / forgetful vs mental power / mental clarity;
Alone / withdrawn vs desire to be touched, caressed; and
Prostration, exhaustion vs energetic.
On the physical sphere there were marked symptoms produced in the head
area with a wide range of headaches. Eye symptoms were also vast in the
inflammation, heaviness and ptosis of the eyes. Throat symptoms manifested
as pharyngitis and sore throat. Toxicological symptoms included vertigo,
ptosis, pain in different areas, inflammation, vomiting, blurred vision, slurred
speech, difficulty breathing and difficult swallowing. Back pain and lower back
pain were also key symptoms. Sleep difficulties and sleepiness were
experienced by provers.
Dream symptoms were the most prevalent in this proving. Dreams were
repetitive in provers in the dreams of changing places and being in many
places and unfamiliar places. There were marked dreams of sexuality, death,
spirituality, friendships, colours, snakes, weddings, fighting and killings and
past recollections.
Conclusion
Dendroaspis angusticeps 30CH thus did produce clearly observable signs
and symptoms in healthy volunteers as hypothesised. The correlation of the
proving symptoms to the toxicology of the venom and the doctrine of
signatures also illustrated the themes of this remedy and aided in
development of the remedy picture for this new addition to the knowledge
base of indigenous South African substances and their homoeopathic
application. / M
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An evaluation of the homoeopathic drug proving of Naja Mossambica in the light of a doctrine of signatures analysis and a comparison between the proving symptons and the venom toxicologyTaylor, Liesel January 2004 (has links)
Thesis (M.Tech.: Homoeopathy)-Dept of Homoeopathy, Durban Institute of Technology, 2004
xiv, 154 leaves : ill. ; 30 cm / This study was conducted by administering Naja mossambica 30CH (a homoeopathic remedy derived from the venom of the Mozambican spitting cobra) to healthy individuals in order to elicit and document the resulting mental and physical symptomology. These symptoms were compared to the toxicology of Naja mossambica venom as well as a doctrine of signatures analysis of the snake in order to expand and clarify the remedy picture.
Existing knowledge of the venom toxicology gives a clear indication of the organs and body systems that the substance has an affinity for. Many poisonous substances used homoeopathically rely heavily on inferences made from the toxicology of the substance, as much of the gross pathology in the symptom picture cannot safely be elucidated in a proving. The aim of this study was to determine the sphere of action of Naja mossambica by utilising symptoms obtained from the proving and from the toxicology of the venom. This was done in order to determine the remedy's usefulness in a homoeopathic clinical setting by expanding our understanding of the substance and thereby facilitating the treatment of disease based on the law of similars.
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A homoeopathic drug proving of Bitis atropos with a subsequent comparison to venom toxicology and related remediesBrijnath, Shraddha 28 May 2014 (has links)
Submitted in partial compliance with the requirements of the Master’s Degree in Technology: Homoeopathy, Durban University of Technology. 2013. / This study was a homoeopathic drug proving of Bitis atropos 30CH (derived from Berg adder venom) with a subsequent comparison of the proving symptoms to known venom toxicology and existing remedies from the materia medica, that on repertorisation, yielded the greatest similarities in the Mental, General, Physical and unique symptomatology of Bitis atropos.
Methodology : The proving was carried out in the form of a double-blinded, placebo controlled trial on healthy subjects who were administered the proving substance or placebo. The resultant influence of this substance on the health of provers (i.e. symptoms produced) was recorded in journal format and formed the materia medica and ultimately the clinical indications thereof according to the Law of Similars. Twenty eight healthy consenting provers who meet the inclusion criteria (Appendix B), were randomly split into two groups, one being the experimental group comprising 22 provers, and the other a placebo control group comprising 6 provers. This was further split between the researcher and co-researcher, each responsible for 11 provers receiving verum and 3 receiving placebo. The researchers and the individual provers were unaware of their respective group allocation and the provers were unaware of the identity of the proving substance.
The fresh venom sourced from a wild, Berg adder, was processed according to the German Homoeopathic Pharmacopoeia (Appendix G) to produce the 30CH Homoeopathic potency thereof. Six lactose powders were dispensed to each prover (either placebo or verum) and taken sublingually three times a day or until the onset of symptoms. Symptoms were recorded by the provers in journals over 4 weeks and were closely supervised by the researcher.
When the symptoms subsided, the combined journals were collected, collated, analysed, interpreted and validated. Accepted symptoms were converted to materia medica and Repertory format.
Results : The proving yielded a total of 903 rubrics, of which 18 were newly created. The systems mostly affected were Dreams, Mind, Head and Eye. Comparison of proving symptoms to that of venom toxicology, as seen in case studies of envenomation by Bitis atropos, yielded similar results, as the sensations experienced in provers closely matched that of known venom toxicology. On repertorisation of the proving symptoms, the existing remedies that were closely related were Sepia officinalis, Lachesis mutus and Argentum nitricum. Further repertorisation of toxicological symptoms indicated a further relation to Belladonna, Natrum muriaticum and Hyoscyamus niger.
Conclusion : Clearly observable signs and symptoms were produced by healthy provers in response to administration of Bitis atropos 30CH, in addition there was a significant degree of similarity between proving symptoms and that of known toxicology of the crude substance. The researcher identified Sepia officinalis, Lachesis mutis and Argentum nitricum as the three most similar existing homoeopathic remedies and a detailed comparison thereof was conducted. A further repertorisation of the toxicological symptoms of envenomation by the snake, yielded the remedies Belladonna, Natrum muriaticum and Hyoscyamus niger which were also compared to Bitis atropos.
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A pharmacological characterisation of death adder (Acanthophis Spp.) venoms and toxinsWickramaratna, Janith C. January 2003 (has links)
Abstract not available
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An evaluation of the homoeopathic drug proving of Naja Mossambica in the light of a doctrine of signatures analysis and a comparison between the proving symptons and the venom toxicologyTaylor, Liesel January 2004 (has links)
Thesis (M.Tech.: Homoeopathy)-Dept of Homoeopathy, Durban Institute of Technology, 2004
xiv, 154 leaves : ill. ; 30 cm / This study was conducted by administering Naja mossambica 30CH (a homoeopathic remedy derived from the venom of the Mozambican spitting cobra) to healthy individuals in order to elicit and document the resulting mental and physical symptomology. These symptoms were compared to the toxicology of Naja mossambica venom as well as a doctrine of signatures analysis of the snake in order to expand and clarify the remedy picture.
Existing knowledge of the venom toxicology gives a clear indication of the organs and body systems that the substance has an affinity for. Many poisonous substances used homoeopathically rely heavily on inferences made from the toxicology of the substance, as much of the gross pathology in the symptom picture cannot safely be elucidated in a proving. The aim of this study was to determine the sphere of action of Naja mossambica by utilising symptoms obtained from the proving and from the toxicology of the venom. This was done in order to determine the remedy's usefulness in a homoeopathic clinical setting by expanding our understanding of the substance and thereby facilitating the treatment of disease based on the law of similars.
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Avaliação da viabilidade do selante de fibrina derivado de veneno de serpente como arcabouço biológico para células-tronco mesenquimais de medula óssea de ratosGasparotto, Vinicius Peron de Oliveira [UNESP] 10 September 2012 (has links) (PDF)
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gasparotto_vpo_me_botfm.pdf: 823732 bytes, checksum: 72bbebb42d3239b78a28393a5aa3b0b7 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Universidade Estadual Paulista (UNESP) / O estudo avaliou a viabilidade in vitro do biomaterial “Selante de Fibrina derivado de veneno de serpente” (SF), como arcabouço para células tronco mesenquimais (CTMs) de ratos. O SF é um material caracterizado como adesivo biológico, e foi produzido e fornecido pelo Centro de Estudos de Venenos e Animais Peçonhentos, CEVAP, Brasil. As CTMs foram coletadas a partir da medula óssea de fêmures e tíbias de ratos e foram caracterizadas por meio de citometria de fluxo com auxilio de marcadores positivos: CD 44 e CD 90 (CTMs) e marcador negativo: CD 34 (células tronco hematopoiéticas). Cultivos foram induzidos para diferenciarem-se em linhagens específicas (osteogênico, condrogênico e adipogênico). Para avaliação do crescimento in vitro e a viabilidade celular em conjunto ao biomaterial foram utilizadas microscopia óptica de luz invertida, microscopia de fluorescência e microscopia eletrônica de varredura e transmissão. O SF em contato com as CTMs não induziu a diferenciação espontânea para as linhagens osteogênica, condrogênica e adipogênica. As diferentes técnicas de avaliação microscópica utilizadas mostraram que o SF foi capaz de realizar a captura e manutenção das CTMs e houve interação das células com o interior e superfície do biomaterial. Portanto, a coleta, o cultivo e a caracterização das CTMs de ratos foram possíveis. O SF mostrou-se eficiente como arcabouço biológico e interagiu com as células tronco mesenquimais mantendo-as viáveis, oferecendo-se como uma ferramenta de uso clínico-cirúrgico alternativa para processos regenerativos visando terapias mais eficientes / This study evaluated the in vitro viability of biomaterial Fibrin Sealant (FS) derived from snake venom as a scaffold for rat mesenchymal stem cells (MSCs). The FS is characterized as a biological adhesive material, and is produced and was supplied by the Center for the Study of Venoms and Venomous Animals, CEVAP, Brazil. MSCs were collected from the bone marrow of femurs and tibias of rat and were characterized using flow cytometry with CD 44 and CD 90 positive markers (MSC) and CD34 negative marker (mononuclear stem cells). Cultivations were induced to differentiate into specific cell lineage (osteogenic, chondrogenic and adipogenic). To evaluate the in vitro growth and cell viability with the biomaterial were used inverted light optical microscopy, fluorescence microscopy and scanning electron microscopy and transmission. The SF did not cause the spontaneous differentiation in contact with MSCs to osteogenic, chondrogenic and adipogenic lineage. Different microscopy techniques showed that the SF was able to accomplish the capture and maintenance of MSCs and there was interaction with the cell interior and surface of the biomaterial. Finally, the collection, cultivation and characterization of rat MSCs were possible. The SF was effective as a biological scaffold and interacted with the MSCs keeping them viable offering itself as a tool for clinical and surgical alternative providing clinical and surgical therapies more efficient for regenerative processes
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Avaliação da viabilidade do selante de fibrina derivado de veneno de serpente como arcabouço biológico para células-tronco mesenquimais de medula óssea de ratos /Gasparotto, Vinicius Peron de Oliveira. January 2012 (has links)
Orientador: Rui Seabra Ferreira Junior / Coorientador: João Ferreira de Lima Neto / Banca: Fernanda da Cruz Landim Alvarenga / Banca: Alexandre Leite Rodrigues de Oliveira / Resumo: O estudo avaliou a viabilidade in vitro do biomaterial "Selante de Fibrina derivado de veneno de serpente" (SF), como arcabouço para células tronco mesenquimais (CTMs) de ratos. O SF é um material caracterizado como adesivo biológico, e foi produzido e fornecido pelo Centro de Estudos de Venenos e Animais Peçonhentos, CEVAP, Brasil. As CTMs foram coletadas a partir da medula óssea de fêmures e tíbias de ratos e foram caracterizadas por meio de citometria de fluxo com auxilio de marcadores positivos: CD 44 e CD 90 (CTMs) e marcador negativo: CD 34 (células tronco hematopoiéticas). Cultivos foram induzidos para diferenciarem-se em linhagens específicas (osteogênico, condrogênico e adipogênico). Para avaliação do crescimento in vitro e a viabilidade celular em conjunto ao biomaterial foram utilizadas microscopia óptica de luz invertida, microscopia de fluorescência e microscopia eletrônica de varredura e transmissão. O SF em contato com as CTMs não induziu a diferenciação espontânea para as linhagens osteogênica, condrogênica e adipogênica. As diferentes técnicas de avaliação microscópica utilizadas mostraram que o SF foi capaz de realizar a captura e manutenção das CTMs e houve interação das células com o interior e superfície do biomaterial. Portanto, a coleta, o cultivo e a caracterização das CTMs de ratos foram possíveis. O SF mostrou-se eficiente como arcabouço biológico e interagiu com as células tronco mesenquimais mantendo-as viáveis, oferecendo-se como uma ferramenta de uso clínico-cirúrgico alternativa para processos regenerativos visando terapias mais eficientes / Abstract: This study evaluated the in vitro viability of biomaterial Fibrin Sealant (FS) derived from snake venom as a scaffold for rat mesenchymal stem cells (MSCs). The FS is characterized as a biological adhesive material, and is produced and was supplied by the Center for the Study of Venoms and Venomous Animals, CEVAP, Brazil. MSCs were collected from the bone marrow of femurs and tibias of rat and were characterized using flow cytometry with CD 44 and CD 90 positive markers (MSC) and CD34 negative marker (mononuclear stem cells). Cultivations were induced to differentiate into specific cell lineage (osteogenic, chondrogenic and adipogenic). To evaluate the in vitro growth and cell viability with the biomaterial were used inverted light optical microscopy, fluorescence microscopy and scanning electron microscopy and transmission. The SF did not cause the spontaneous differentiation in contact with MSCs to osteogenic, chondrogenic and adipogenic lineage. Different microscopy techniques showed that the SF was able to accomplish the capture and maintenance of MSCs and there was interaction with the cell interior and surface of the biomaterial. Finally, the collection, cultivation and characterization of rat MSCs were possible. The SF was effective as a biological scaffold and interacted with the MSCs keeping them viable offering itself as a tool for clinical and surgical alternative providing clinical and surgical therapies more efficient for regenerative processes / Mestre
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