Objective of the theses was to determine the botanical origin of bee-collected pollens using pollen analysis. The beehive, from where the pollen came from, is located in Hlavatce, which is 20 km far from České Budějovice. Bee-collected polens was obtained via pollen trap, which was put on the beehive once a week for 24 hours between 17th March to 6th June 2017. Pollens were dried, sorted according to the color shades, and the partial color samples were weighed on the analytical scales. The pollen analysis took place at a microscope magnification of 400x. The characteristics of exine (sculpture) and quantity and type of apertures were observed. The size of the grain, which was measured always in 50 pollen grains, helped to classify the plant species. Using the Shannon-Wiener Diversity Index, the bee's preferences were assessed in individual samples. The work is supplemented with photos of selected pollen grains.

In palaeopalynology oaks are rarely identified beyond the generic level. In order to recognize fossil evergreen and deciduous oak pollen, we compiled distinguishing criteria from the literature, most under the SEM, on the living oaks. One of the most significant criteria is the nature of the sculpturing under the SEM. Evergreen oak pollen exhibit at least four kinds of sculpturing, viz. rod-like elements, uniformly fine granules, scabrate-verrucate, and rugulate micromorphology. The first two types of sculpturing are mostly limited to the evergreen oaks and can be used to identify fossil evergreen oak pollen, while the last two types can also be seen in many deciduous oaks. On the other hand, deciduous oak pollen grains have relatively uniform sculpturing, with only two types encountered: scabrate-verrucate and rugulate sculpturing. This sculpturing is mainly present in deciduous oaks, despite its occurrence in some evergreen oaks. An absolute discrimination between evergreen and deciduous oak pollen is thus impossible and would require additional evidence in the form of macrofossils. The diagnostic criteria are applied to a Miocene pollen assemblage from Zhejiang Province, eastern China and demonstrate the high diversity of fossil oaks in the vegetation. The fossil pollen includes three morphotaxa of deciduous origin, two morphotaxa of evergreen habit, and three oaks whose habit is uncertain.

No description available.

Gesneriaceae é uma das famílias mais antigas da ordem Lamiales e possuí aproximadamente 150 gêneros, entre eles, Sinningia Nees, com um grande número de espécies endêmicas do Brasil. Foram estudados os grãos de pólen de 27 espécies brasileiras de Sinningia com o objetivo de caracterizar e identificar dados polínicos que possam auxiliar na taxonomia da família, ampliando os conhecimentos sobre a diversidade polínica do gênero, a fim de fornecer subsídios para o melhor entendimento das relações entre as espécies e da evolução dos caracteres polínicos, assim como relacionar os dados obtidos nesse trabalho com as síndromes de polinização descritas para o gênero. O material analisado foi obtido a partir de espécimes depositados nos herbários SP, MBM, MBML, ESA, UEC e INPA e de material de cultivo do Sítio Primavera em Mogi das Cruzes SP. Os grãos de pólen foram acetolisados, medidos, fotografados em microscopia óptica, microscopia eletrônica de varredura e transmissão e descritos qualitativamente. Os dados quantitativos foram analisados por meio de estatísticas descritiva e multivariada. Para as espécies brasileiras de Sinningia estudadas foram observadas variações entre os grãos de pólen, principalmente quanto ao tamanho, âmbito, forma, comprimento dos colpos e ornamentação. Foram encontrados grãos de pólen pequenos, pequenos à médios e médios; isopolares; âmbito circular, circular-lobados, subcircular e subtriangular; oblato-esferoidais, prolato-esferoidais, subprolatos e prolatos; área polar muito pequena, pequena ou grande; 3-colporados; colpos muito curtos, curtos, longos e muito longos e estreitos; colpos às vezes contritos na região mediana; endoabertura lolongadas; exina microrreticulada heterobrocada e homobrocada. Os dados obtidos confirmam o caráter estenopolínico do gênero, uma vez que as diferenças encontradas nos grãos de pólen são discretas e não permitem a identificação intragenérica. / Gesnericeae is one of the oldest families of the order Lamiales and has approximately 150 genera, among them, Sinningia Nees, that owns a great number of species endemic of Brazil. We studied the pollen grains of 27 Brazilian Sinningia with the objective of characterizing and identifying pollen data that could aid in family taxonomy, knowledge on pollen diversity of the genus,in order to provide support for a better understanding of the relationships between species and the evolution of pollinic characters, as well as to relate the data obtained in this work to the pollination syndromes described for the genus. The material analyzed was obtained from the specimens deposited in the herbaria SP, MBM, MBML, ESA, UEC and INPA and of cultive material of the Sítio Primavera in Mogi das Cruzes SP. The pollen grains were acetolysed, measured, photographed under light microscopy, scanning and transmission electron microscopy and described qualitatively. The quantitative data were analyzed by descriptive and multivariate statistics. For the Brazilian Sinningia species studied, variations were observed between pollen grains, mainly in the size, amb, shape, length colpi and ornamentation. The pollen grains are small, small to medium and medium; isopolar; circular, circular-lobate, subcircular and subtriangular amb; oblate-spheroidal, prolate-spheroidal, subprolate and prolate; polar area is very small, small or large; 3-colporate; very short, short, long and very long colpi and narrows; sometimes contrite in the median region; lolongate endoaberture; heterobrocate and homobrocate microrreticulate ornamentation. The data obtained confirm the stenopalynous character of the genus, since the differences found in the pollen grains are discrete and do not allow intrageneric identification.

Submitted by Francine Silva (francine.silva@unipampa.edu.br) on 2016-09-27T12:42:09Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Morfologia Polínica de Táxons de Poaceae do Rio Grande do Sul Uma abordagem para distinguir vegetações campestres e florestais no Sul do Brasil.pdf: 1427510 bytes, checksum: d3bfb6022f41971b6b785d86f5c494af (MD5) / Made available in DSpace on 2016-09-27T12:42:09Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Morfologia Polínica de Táxons de Poaceae do Rio Grande do Sul Uma abordagem para distinguir vegetações campestres e florestais no Sul do Brasil.pdf: 1427510 bytes, checksum: d3bfb6022f41971b6b785d86f5c494af (MD5) Previous issue date: 2015-07-16 / Grãos de pólen de Poaceae identificados em registros quaternários podem ser somente indicativos da vegetação de Campo e não permite a obtenção de maiores inferências ambientais e ecológicas devido à uniformidade dos grãos de pólen. Neste sentido, foram realizadas análises em microscopia óptica e eletrônica de varredura em diferentes táxons contemplando-se todas as tribos e subfamílias de Poaceae do Rio Grande do Sul para avaliar se seus grãos de pólen podem ser distinguidos em relação: (i) ao tipo de vegetação (Campo e Floresta), (ii) ao metabolismo fotossintético C3 e C4, (iii) ao tipo de hábito entre as espécies florestais, (iv) a diferentes níveis taxonômicos (gênero, tribo, ubfamília). São apresentadas informações polínicas sobre 68 espécies de Poaceae istribuídas em vegetações campestres ou florestais do Rio Grande do Sul. A análise por microscopia óptica e eletrônica de varredura permitiu distinguir os grãos de pólen em relação ao tipo de vegetação em que os táxons estão distribuídos (Campo e Floresta). Grãos de pólen de espécies florestais apresentaram tamanho maior do que táxons campestres, assim como espécies com metabolismo fotossintético C3 demonstraram tendência a apresentar grãos de pólen maiores que táxons com metabolismo C4. Padrões nos tamanhos dos grãos de pólen de táxons florestais de acordo com o hábito também foram obtidos, destacando-se o tamanho distinto dos grãos de pólen da tribo Bambuseae. Com base nos resultados, os grãos de pólen de Poaceae podem ser utilizados como indicadores ambientais para melhor caracterização das dinâmicas e reconstituições vegetacionais no sul do Brasil. / Poaceae pollen grains identified in quaternary records can only indicate the grassland vegetation and does not allow more environmental and ecology inferences due to the uniform morphology of the pollen grains. In this sence, analyses were performed in optical and scanning electron microscopy in different taxa representing all tribes and subfamilies of Poaceae family of the Rio Grande do Sul to assess whether their pollen grains can be distinguished in relation: (i) to the type of vegetation (grassland and forest), (ii) to the photosynthetic metabolism C3 and C4, (iii) to the type of habit between forest species, (iv) to different taxonomic levels (genera, tribe, subfamily). Are presented pollen information about 68 species of Poaceae family distributed in the grassland or forest vegetation of the Rio Grande do Sul. Analysis by optical and scanning electron microscopy allowed to distinguish the pollen grains in relation to the type of vegetation of species (grassland and forest). Pollen grains of forest species showed larger size than grasslands taxa, as well as species with photosynthetic metabolism C3 showed trend to have pollen grains larger than taxa with metabolism C4. Patterns in sizes of pollen grains of the forest species in relation to the habit were also obtained, highlighting the distintic size of pollen grains of the ambuseae tribe. Based on the results, the Poaceae pollen grains can be used as environmental indicators for better characterization of the dynamics and reconstructions vegetation in southern Brazil.

Στόχος της παρούσας εργασίας είναι η συμβολή στη δημιουργία ενός γυρεολογικού άτλαντα για τα φυτά της Πανεπιστημιούπολης Πατρών. Γι’ αυτό το σκοπό, στη διάρκεια ενός χρόνου συλλέχθηκαν και αναγνωρίστηκαν 150 δείγματα από την Πανεπιστημιούπολη και δημιουργήθηκε ένα «Herbarium». Από κάθε είδος συλλέχθηκε ένας μεγάλος αριθμός ανθέων για επεξεργασία στο εργαστήριο. Στη διαδικασία αυτή ελήφθη το γυρεολογικό υλικό και ετοιμάστηκαν τα μικροσκοπικά παρασκευάσματα με τη μέθοδο ακετόλυσης Erdtman. Μελετήθηκαν οι εξής οικογένειες Apocynaceae (1), Boraginaceae (7), Buxaceae (1), Capparaceae (1), Caprifoliaceae (1), Caryophyllaceae (2), Compositae (15), Convolvulaceae (2), Cruciferae (2), Dipsacaceae (2) Euphorbiaceae (1), Geraniaceae (1), Gramineae (2), Iridaceae (2), Labiatae (9), Leguminosae (18), Liliaceae 6, Malvaceae (2), Oleaceae (3), Oxalidaceae (1), Papaveraceae (2), Plantaginaceae (1), Pinaceae (1), Pittosporaceae (1), Primulaceae (1), Ranunculaceae (4), Resedaceae (1), Scrophulariaceae (2), Solanaceae (1) Umbelliferae (3) Urticaceae (2), Verbenaceae (2). Μετά έγιναν για κάθε είδος τουλάχιστον 150 μετρήσεις των χαρακτηριστικών διαστάσεων των γυρεοκόκκων του που είναι η πολική (Ρ) και η ισημερινή (Ε) απόσταση και ο λόγος της πολικής προς την ισημερινή απόσταση (Ρ/Ε). Στη συνέχεια πραγματοποιήθηκε η στατιστική επεξεργασία των μετρήσεων και η εξαγωγή των μέσων τιμών και της διασποράς τους. Για κάθε είδος, περιγράφτηκαν τα μορφολογικά και ανατομικά χαρακτηριστικά στοιχεία των γυρεοκόκκων. Τέλος, η μορφολογική περιγραφή του γυρεοκόκκου ολοκληρώθηκε με τη φωτογράφηση στο Ο.Μ. των παρασκευασμάτων. Σε μερικά είδη παρατηρήθηκαν διαφορές ως προς τις βιβλιογραφικά δεδομένα από άλλες περιοχές, όσον αφορά το ανάγλυφο, την μορφολογία των ανοιγμάτων (Anemone pavonina) το μέγεθος των γυρεοκόκκων (Vicia villosa, Pimpinella peregrina, Onopordon illyricum κ. α.) Στο Onopordon illyricum L. βρήκαμε γυρεοκόκκους με Ρ = 59, 35 μm, Ε = 55,14 μm ενώ στη βιβλιογραφία αναφέρονται Ρ = 40 – 45 μm και Ε = 39 - 45 μm. Στο είδος Anemone pavonina Lam. η βιβλιογραφία αναφέρει πολυπορώδεις γυρεοκόκκους ενώ στο δικό μας υλικό οι γυρεόκοκκοι φαίνονται παντοκολπικοί. Στην Vicia villosa Roth.. βρήκαμε Ρ = 42,32 μm και Ε = 20,61 μm ενώ βιβλιογραφικά αναφέρονται τιμές Ρ = 28 – 35 μm και Ε = 14 – 21 μm. Αυτή η διαφοροποίηση είναι μια γεωγραφική διαφοροποίηση που σχετίζεται και συνοδεύεται και από μορφολογικές και γενετικές διαφορές. / The aim of the present study is the construction of a pollen atlas with regard to the plant species in the area of the University of Patras, Greece. For this purpose, 120 different samples were collected and characterized and a «Herbarium» was created. From each of the species, a big number of flowers were collected for further processing in the lab. Fresh polliniferous material was procured randomly from several plants growing in the field of the University of Patras. For light optical microscope (LO) studies pollen were acetolyzed following the protocol of Erdtman (1952). The plant taxa studied here belongs to the following families: Apocynaceae (1), Boraginaceae (7), Buxaceae (1), Capparaceae (1), Caprifoliaceae (1), Caryophyllaceae (2), Compositae (15), Convolvulaceae (2), Cruciferae (2), Dipsacaceae (2) Euphorbiaceae (1), Geraniaceae (1), Gramineae (2), Iridaceae (2), Labiatae (9), Leguminosae (18), Liliaceae 6, Malvaceae (2), Oleaceae (3), Oxalidaceae (1), Papaveraceae (2), Plantaginaceae (1), Pinaceae (1), Pittosporaceae (1), Primulaceae (1), Ranunculaceae (4), Resedaceae (1), Rubiaceae (1), Scrophulariaceae (2), Solanaceae (1) Umbelliferae (3) Urticaceae (2), Verbenaceae (2). At least 150 measurements were taken for each plant taxon pollen grain preparation, in order to calculate the mean value of the polar (Ρ) and equatorial (Ε) dimensions of the taxon’ s pollen grains, as well as, the ratio Ρ/Ε. Statistical analysis was performed and the mean values were recorded. The morphology and anatomy of each species pollen grains were determined. The study was completed by taking LM pictures of all pollen grains. In some of the species studied in the area we have noticed some differences between the pollen grains dimensions, or even the morphology of the pollen grain surface, and of the same taxa that are described from other countries (Anemone pavonina Lam., Onopordon illyricum L., Vicia villosa Roth., Pimpinella peregrina L.) In Onopordon illyricum L. we have found pollen grains with P = 59,35 μm, Ε = 55,14 μm and the same species from Spain is described with P = 40 – 45 μm ανδ Ε – 39 – 45 μm. Anemone pavonina Lam. is referred to have poliporate pollen grains, in our material it seems to have policolpate pollen grains. In Vicia villosa Roth. From Spain is referred P = 28 – 35 μm and E = 14 – 21 μm, and we found P = 42,32 μm and E = 20,61 μm. This might be a geographic differentiation that is normal in pollen grains and is related with other morphological and genetic differences.

Objective of the work was to identify with using pollen analysis botanical origin of bee-colected pollens collected from beehive station Lískovec near the town Volary in the Protected Landscape Area of Šumava. Bee-collected pollens were collected once a week from one honey bee colony with using of a pollen trap. The time of collection was summer and late summer period from 22 June to 11 September 2010. Bee-collected pollens were dried, categorized by color, weighed and observed using the microscope. Pollen grains were determined by the number and type of apertures, shape, sculpture and size. Size of pollen grains was measured on at least 50 pollen grains of one type. In each microscopic slide were counted at least 500 pollen grains. The proportion of plant species in the sample was expressed as a percentage and the importace of plant species was evaluated in the diet of honey bees in the area of interest. Selected samples of bee-collected pollens from beehive station Lískovec and also from beehive station Dobčice were investigated for protein content and amino acid composition. Pollen belonging to Polemonium coeruleum, Trifolium pratense and Trifolium repens was evaluated as an important source of protein and amino acids for honey bees. The concentration of essential amino acids expressed as percentages of total amount of amino acids did not differ significantly between samples of bee-collected pollen. Another objective of the work was to compare the results from the area of interest with the results processed in the similar study from beehive station Dobčice in the northern area of the landscape Blanský les. The work also includes statistical evaluation of dominance, frequency, Shannon-Wiener index of diversity and variability between samples and species. The work is complemented by photographs of selected pollen grains.

Objective of the work was to identify with using pollen analysis botanical origin of bee pollens. Pollen analysis consisted in the distribution of samples to individual sub-samples . Samples were weighed and a part of them was dissolved in a solution of glycerin and water. Dissolved samples were viewed under a microscope. Pollen was removed by the beekeeper Mgr .Milan Trhlín with using a device called pollen catcher. The samples were collected in the year 2011 in the spring and early summer period from beehive location on the hill Lískovec, of the protected landscape Šumava 1,8 km west of city Volary, at a time of 25. march to 24. june. Another objective of the work was with using phytocenology study, in the distance 1,5 km from the beehive habitat, evaluate the structure of plant association from the point of nutrition honeybees. The work also includes evaluation preference bees for individual plant species. There were detected, that bees prefer the plants that provide the best digest pollen and the plants which grows up to 1,5 km from beehive habitat. There were distinguised 32 types of pollen grains.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-09-26T21:20:40Z No. of bitstreams: 1 THA?LA VIEIRA ALVES DOS SANTOS_v_final_ficha-2.pdf: 5538347 bytes, checksum: f6306a15edb73efd871da71bcbbdd53c (MD5) / Made available in DSpace on 2016-09-26T21:20:40Z (GMT). No. of bitstreams: 1 THA?LA VIEIRA ALVES DOS SANTOS_v_final_ficha-2.pdf: 5538347 bytes, checksum: f6306a15edb73efd871da71bcbbdd53c (MD5) Previous issue date: 2016-03-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Portulaca hirsutissima is a succulent herb that occurs in open fields, sandy or rocky soil in distinct regions in Brazil, belonging to the Portulacaceae family. We analyzed the external morphology, seeds and pollen grains in representatives of this species and related morphotypes along their known distribution. We observed a consistent variation in their micromorphology and two new species are here described and illustrated. Portulaca giuliettiae occurs in Eastern Brazil, from Paraiba to Rio de Janeiro and P. goiasensis is restricted to the Chapada dos Veadeiros, in Central Brazil, while P. hirsutissima is now considered as restricted to Minas Gerais State. / Portulacaceae ? uma fam?lia com ca. de 100 esp?cies de ervas suculentas, com distribui??o em todo o mundo, tendo como centro de diversidade as Am?ricas. O grupo apresenta taxonomia complexa, com caracteres macromorfol?gicos vari?veis e muitas vezes de dif?cil interpreta??o. Neste trabalho foram analisados caracteres micromorfol?gicos das esp?cies que ocorrem no Brasil, como a ornamenta??o das sementes e morfologia pol?nica, em busca de melhor compreender os limites taxon?micos entre as esp?cies. As varia??es observadas envolveram a presen?a de poros ou colpos distribu?dos em todo gr?o de p?len, permitindo a identifica??o de tr?s tipos pol?nicos: pantossincolpado, com colpos organizados em pent?gonos; pantossincolpado, com colpos organizados em espiral; e pantoporado, com poros isolados e organizados em poliedros. A ornamenta??o das sementes forneceu os caracteres taxon?micos mais importantes e permitiu a separa??o de todas as esp?cies analisadas, apoiando inclusive a recente proposi??o de duas novas esp?cies end?micas do Brasil, relacionadas a P. hirsutissima e tamb?m distintas pela morfologia pol?nica.

Foram estudadas a morfologia polínica de 20 espécies de Beslerieae Bartl. (Anetanthus, Besleria e Tylopsacas) e quatro espécies de Napeantheae Wiehler (Napeanthus), nativas no território brasileiro e encontradas principalmente nos biomas da Amazônia e/ou Mata Atlântica. O objetivo deste trabalho é contribuir com a caracterização morfológica das espécies, identificando dados polínicos que possam auxiliar na taxonomia do grupo, ampliando desta forma os conhecimentos sobre a diversidade polínica nos gêneros estudados e fornecendo subsídios para o melhor entendimento das relações entre as tribos brasileiras de Gesneriaceae e da evolução dos caracteres polínicos nestes gêneros. Também foi discutida a relação entre a morfologia polínica de Besleria e sua distribuição fitogeográfica. O material analisado foi obtido a partir de espécimes depositados nos herbários INPA, SP e MBM. Os grãos de pólen foram acetolisados, medidos, fotografados em microscopia óptica, microscopia eletrônica de varredura e transmissão e descritos qualitativamente. Os dados quantitativos foram analisados por meio de estatísticas descritiva e multivariada. Os grãos de pólen são mônades; isopolares; de tamanho pequeno; com grande variação de forma, podendo ser, oblatos, suboblatos, oblatos-esferoidais, prolatos-esferoidais, subprolatos ou prolatos; âmbitos circulares, circulares-lobados, subcirculares a subtriangulares. As aberturas são 3-colpadas, 3- (4) colpadas ou 3-colporadas; colpos longos, curtos ou muito curtos; estreitos a largos; extremidades afiladas ou arredondadas, algumas vezes possuindo margem, colpo constrito ou membrana ornamentada; endoabertura predominantemente lolongada, as vezes circulares. Ornamentação da exina fossulada, microrreticulada, microrreticulada-rugulada, microrreticulada-fossulada, rugulada, rugulada-perfurada. Exina variando de muito fina, fina a espessa, sexina sempre mais espessa que a nexina. Variações no tipo de abertura e nos padrões da ornamentação da exina dos grãos de pólen contribuíram para a distinção das espécies estudadas e confirmam o caráter euripolínico de Beslerieae e Napeantheae. / The pollen morphology of 24 Brazilian species of Beslerieae Bartl. (Anetanthus, Besleria and Tylopsacas) and Napeantheae Wiehler (Napeanthus) was studied, these species are native to the Amazon and / or Atlantic Forest biomes. The objective of this study is to contribute to the morphological characterization of the species, identifying pollen data that may help in the taxonomy of the group, thus increasing the knowledge about the pollen diversity and evolution in the studied genera and providing subsidies for a better understanding of the relations between the Brazilian tribes of Gesneriaceae. Also we discussed the relationship between the pollen morphology of Besleria and its phytogeographic distribution. The material analyzed was obtained from specimens deposited in the INPA, SP and MBM herbaria. The pollen grains were acetolysed, measured, photographed under light microscopy, scanning and transmission electron microscopy and described qualitatively. Quantitative data were analyzed using descriptive and multivariate statistics. The pollen grains are monads; isopolar; small size; with variation in shape, oblate, suboblate, oblate-spheroidal, prolate-spheroidal, subprolate or prolate; circular, circular-lobed, subcircular to subtriangular amb. The apertures are 3-colpate, 3-(4) colpate or 3-colporate; long, short or very short colpi and narrow to wide; with or without margo, rounded or tapered at the polar ends, sometimes constricted or with ornate membrane; endoapertures lolongate or circular. Exine fossulate, microrreticulate, microreticulate-rugulate, microreticulate-fossulate, rugulate, rugulate-perforate. Very thin, thin to thick exine, sexine always thicker than nexin. Variations in the type of aperture and patterns of exine ornamentation of the pollen grains contributed to the distinction of the species studied and confirmed the eurypalynous character of Beslerieae and Napeantheae.