Fresh pork loins were cut for three replications and randomly assigned to one of seven treatments: 1) polyvinylchloride (PVC) packaged, 2) vacuum packaged (Vac-pack), 3) 0.2 mL of Tris-Buffer, 4) 0.2 mL of high leghemoglobin high pH (HLbHpH), 5) 0.2 mL of high leghemoglobin low pH (HLbLpH), 6) 0.2 mL of low leghemoglobin high pH (LLbHpH), and 7) 0.2 mL of low leghemoglobin low pH (LLbLpH). Treatments 3 through 7 also were vacuum packaged. Leghemoglobin (Lb) protein treatments improved (P<0.05) the visual color of vacuum packaged pork at the point of purchase. CIE L* values were not affected by HLbHpH, LLbHpH or LLbLpH treatments. However, the HLbLpH treatment was lighter (P<0.05) than the vacuum packaged control treatments on days 3, 4, and 5. High Lb (HLb) treatments had a positive effect (P=0.0116) on the CIE a* values on the day of manufacture. HLbLpH treatment eIE b* values were higher (P<0.05) than Vac-pack and buffer controls from day 0 to day 5. On the day of processing, HLb treatments were higher (P=0.0637) in oxymyoglobin than low Lb treatments. Metmyoglobin of HLb treatments was higher (P<0.05) than the Vac-pack and the buffer treatments on the day of manufacture. pH did not affect (P>0.05) sensory or objective color, pigment state, purge or total psychrotrophic aerobic bacterial counts (TPC) throughout the study. Lb treatments TPC were lower (P<0.0l) than nontreated samples at all assessment times. / Master of Science

Master of Science

Univ., Diss.--Hohenheim, 2006.

Canada's commitment to the Kyoto Protocol provides agricultural producers with an opportunity to supply carbon offset credits to a domestic carbon market and receive revenue from the sale of these credits. This study employed the multiple bounded discrete choice method to estimate Quebec hog producers' willingness to accept compensation to adopt two management practices that reduce carbon emissions; i.e. reduced protein feeding and adopting a manure storage cover. The average willingness to accept compensation for reduced protein feeding was $46.71 per tonne of CO2 equivalent and for the manure storage cover was $40.40 per tonne of CO2 equivalent. In addition, hog producers were asked what cost they would be willing to bear if they received $20 per animal unit in carbon offset credit revenue. The average cost they were willing to bear was $11.88. Key factors that influenced producers' decisions were identified. Results can be used to improve the institutional rules and public policy associated with developing a domestic carbon emission trading mechanism. Starting-point and sequencing bias were tested for with the convolution approach. Starting-point bias was found in all the hypothetical situations; while sequencing bias was not found.

The focus of this thesis was on the economic feasibility of three food processing projects. They involved freezing of pork loin and fish using cryogenic freezing and stored at $-$18, $-$12 and $-$7$ sp circ$C, and modified atmosphere packaging (MAP) of pork loin using a combination of nitrogen and carbon dioxide gases, chitosan dip and stored at 5, 10, and 15$ sp circ$C. A production plan with similar layout and capacity was adopted for the Montreal area. The benefit-cost analysis (BCA) of each project was carried out assuming that each plant operated approximately 2000 hours per annum at full capacity. Net present value (NPV) and benefit cost ratio (B/C ratio) at 8% discount rate, and internal rate of return (IRR) were determined for each project. The calculations show the NPV of $2.4 million, $1.8 million, and $3.4 million; B/C ratio of 1.09, 1.09, and 1.10; IRR of 15 %, 13 %, and 18 % for frozen pork loin, frozen fish and MAP pork respectively. All IRRs were higher compared to the 1995 return rates of TSE of 9.7 % and the average return of capital of 8.8 % for some food and beverage industries. These economic values are sensitive to changes in working hours, price of raw materials and finished products. It is also found that MAP products could be stored at temperatures up to 15$ sp circ$C. Frozen products could be stored at temperatures of $-$12$ sp circ$C and below only if the length of distribution chain can be reduced. This would decrease electricity costs up to 36% at each level of distribution. It can be concluded that the three projects are economically profitable.

No description available.

No description available.

No description available.

CAPES / A Salmonella spp. é um dos principais micro-organismos patogênicos envolvidos em Doenças Transmitidas por Alimentos (DTA’s), com destaque para surtos envolvendo a ingestão de carne suína. Nesse sentido, o objetivo deste estudo foi avaliar a segurança alimentar na produção de carne suína e embutidos quanto a presença de Salmonella spp. no processo produtivo. As amostras foram coletadas em um frigorífico que abate suínos e fabrica embutidos, localizado na região dos Campos Gerais - PR. Inicialmente, para padronização da PCR foi necessário determinar um protocolo de extração, bem como, ajustes metodológicos para amplificação de DNA. Para esses testes realizados, foi utilizada amostra de Salmonella spp previamente isolada de alimento. O protocolo de extração testado foi lise térmica e para reação de amplificação foram avaliadas três concentrações de DNA e diferentes temperaturas de hibridização para estabelecimento do padrão ideal. O protocolo escolhido mostrou-se bastante eficiente para extração do DNA de Salmonella spp, pois permitiu a obtenção de DNA em quantidade e com qualidade suficiente para amplificação de bandas. Para a amplificação, a melhor condição encontrada foi a concentração de DNA de aproximadamente 40 ng e uma temperatura de hibridização de57 ºC.Com o intuito de validar a análise molecular via PCR, realizouse um estudo comparativo inicial com a microbiologia convencional para comprovação dos resultados obtidos pela análise molecular. Inicialmente foram escolhidos dezessete pontos durante as diferentes etapas do processo produtivo do frigorífico em estudo. Duas carcaças foram acompanhadas durante todo o processo e amostras foram coletadas, contemplando desde a etapa de escaldagem até o embutimento do produto final. A utilização da técnica de PCR mostrou-se vantajosa nos seguintes aspectos: tempo de análise total de aproximadamente 30 horas; maior sensibilidade comparado ao método convencional. Decorrida a validação, foi realizada nova coleta, contemplando etapas desde pré-abate até a obtenção do embutido, perfazendo um total de 62 amostras, com intuito de avaliar contaminação durante a produção de carne suína e embutidos. Como resultado, foi verificado que 60% das amostras estavam contaminadas por Salmonella spp, em diversas etapas do processo produtivo. A partir dessa avaliação, foram selecionados alguns pontos contaminados e elaborado um plano de ações corretivas, a fim de controlar e diminuir os perigos microbiológicos existentes no local. Através de novas análises via PCR foi possível verificar que o plano de ações foi eficiente em 100% das amostras. / The Salmonella spp is major pathogens involved in Food borne Diseases (FBD), especially outbreaks involving the ingestion of meet and this products. The aim of this study was to evaluate the food safety in pork and sausages production for the Salmonella spp. presence in the process. The samples were collected in a refrigerator that slaughter pigs and manufactures sausages, located in the Campos Gerais - PR. Initially, to standardize the PCR was necessary to determine an extraction protocol, as well as methodological adjustments to the PCR reaction. For these tests it was used sample of Salmonella isolated from food. The extraction protocol was tested by heating process and for amplification reaction were evaluated three different concentration of DNA and hybridization temperatures to establish the ideal standard. The chosen protocol proved to be very efficient for the Salmonella ssp DNA extraction because it allowed obtaining DNA in sufficient quality and quantity for amplification bands. For amplification, the best condition was found a concentration of approximately 40 ng DNA and a hybridization temperature of 57 ° C. In order to validate the molecular analysis by PCR, we carried out a comparative study with the initial conventional microbiology for proof of all results obtained by molecular analysis. Seventeen points were chosen during the different stages of production process. Two carcasses were monitored throughout the procedure and samples were collected, comprising the scalding step to the final product inlay. The PCR usage technique proved advantageous in the following aspects: total analysis time of approximately 30 hours; higher sensitivity compared to conventional. After validation, we performed a new collection, covering stages from pre-slaughter until embedded obtaining, making a total of 62 samples, in order to assess contamination during production of pork and sausages. As a result, it was found that 60% of the samples were contaminated with Salmonella spp, in various stages of production. From this evaluation, we selected some points contaminated and developed a corrective action plan in order to control and reduce microbiological hazards on the premises. Through further analysis by PCR was possible to verify that the action plan was effective in 100% of samples.