Ulm, Univ., Diss., 2008.

Zugl.: Hamburg, Univ., Diss., 2008

Zugl.: Hamburg, Univ., Diss., 2007

Regensburg, Univ., Diss., 2008.

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, 2008. / Submitted by Jaqueline Oliveira (jaqueoliveiram@gmail.com) on 2008-11-17T16:15:35Z No. of bitstreams: 1 DISSERTACAO_2008_ElianaMitikoNumazaki.pdf: 718225 bytes, checksum: 7eee7ae19dfcf31c67f761efd53784b0 (MD5) / Approved for entry into archive by Georgia Fernandes(georgia@bce.unb.br) on 2009-01-28T12:10:07Z (GMT) No. of bitstreams: 1 DISSERTACAO_2008_ElianaMitikoNumazaki.pdf: 718225 bytes, checksum: 7eee7ae19dfcf31c67f761efd53784b0 (MD5) / Made available in DSpace on 2009-01-28T12:10:07Z (GMT). No. of bitstreams: 1 DISSERTACAO_2008_ElianaMitikoNumazaki.pdf: 718225 bytes, checksum: 7eee7ae19dfcf31c67f761efd53784b0 (MD5) / Com o objetivo de avaliar o efeito da utilização de mananoligossacarídeo (MOS) e halquinol (HAL) como aditivos para aves de postura, foram utilizadas 550 poedeiras leves de linhagem Bovans White distribuídas em delineamento fatorial 3X3 em blocos casualizados com 2 controles, totalizando 11 tratamentos com 5 repetições de 10 aves cada, para que fossem avaliados os parâmetros produtivos, qualidade dos ovos, características morfológicas e morfométricas intestinais e níveis séricos de albumina e proteína total. Foram alojadas 2 aves por gaiola, com densidade de 500 cm² para cada uma, com dieta fornecida ad libitum em duas fases: Crescimento Fase I, de 12 a 16 semanas, e Postura Fase II, de 17 a 46 semanas de idade. Os 11 tratamentos avaliados foram: 1) Controle Negativo MOS; 2) Controle Negativo HAL; 3) 20ppm HAL + 0,5kg/ton MOS; 4) 20ppm HAL + 1,0kg/ton MOS; 5) 20ppm HAL + 1,5kg/ton MOS; 6) 30ppm HAL + 0,5kg/ton MOS; 7) 30ppm HAL + 1,0kg/ton MOS; 8) 30ppm HAL + 1,5kg/ton MOS; 9) 40ppm HAL + 0,5 kg/ton MOS; 10) 40ppm HAL + 1,0kg/ton MOS; 11) 40ppm HAL+ 1,5kg/ton MOS. As avaliações produtivas e qualitativas foram realizadas de 17 a 46 semanas de idade, considerando os seguintes períodos: 1) 17 a 22 semanas; 2) 23 a 26 semanas; 3) 27 a 30 semanas; 4) 31 a 34 semanas; 5) 35 a 38 semanas 6) 39 a 42 semanas e 7) 43 a 46 semanas. Não foram observadas diferenças significativas (P<0,05) para a produção de ovos (%), conversão alimentar por dúzia de ovos (kg/dz), peso dos ovos (g), peso relativo da casca, da gema e do albúmen (%), gravidade específica dos ovos e os níveis séricos de albumina e proteína total (g/100ml). Observou-se no Período 2 maior consumo de ração (g/ave/dia) no controle negativo de HAL (Trat 2), apontando-se ponto de menor consumo com a adição de 0,700kg/ton de MOS. A conversão alimentar (kg/kg) foi influenciada pelos aditivos nos períodos 4 e 6 e na média do período experimental, com indicação de mínima conversão alimentar com a inclusão de 0,600kg/ton e 0,725kg/ton de MOS no período 4 e na média do experimento, respectivamente. A adição de 19 a 28ppm de halquinol corresponderam, respectivamente, às maiores taxas de conversão alimentar (kg/kg) obtidas para o Período 6 e a média do experimento. A adição de 1,5kg/ton de MOS e 40ppm de halquinol resultaram nas maiores medidas de espessura da casca para o período 2. Nas aves sacrificadas com 36 e 46 semanas de idade, não se observou efeito dos aditivos sobre o peso do intestino, da moela, do fígado e do pâncreas. Entretanto, indicou-se menor profundidade das criptas (μm) associado à maior relação vilo:cripta para segmentos do duodeno e íleo com a adição de 1,5kg/ton de MOS e 40ppm de halquinol. Os resultados apontaram que HAL e MOS adicionados com níveis mais altos favorecem as características qualitativas dos ovos e os parâmetros intestinais, mas que o MOS em doses inferiores a 1,0kg/ton gerou melhores resultados produtivos. __________________________________________________________________________________________ ABSTRACT / To evaluate the effect of the inclusion of mananoligosaccharyde (MOS) and halquinol (HAL) as additives in laying hens feed, 550 Bovans White pullets were allotted in a 3X3 factorial arrangement design with two negative controls, totalizing 11 treatments, with 5 replicates containing 10 hens each, for the evaluation of productive parameters, egg quality, intestinal morphologic and morphometric characteristics and total serum protein and albumin. Each cage contained 2 hens, considering the stocking density of 500cm², with feed provide ad libitum in two phases: Growth Phase I, from 12 to 16 week, and Production Phase II, from 17 to 46 week. The eleven evaluated treatments were: 1) MOS Negative Control; 2) HAL Negative Control; 3) 20ppm HAL + 0,2kg/ton; 4) 20ppm HAL + 1,0kg/ton MOS; 5) 20ppm HAL + 1,5kg/ton MOS; 6) 30ppm HAL + 0,5kg/ton MOS; 7) 30ppm HAL + 1,0kg/ton MOS; 8) 30ppm HAL + 1,5kg/ton MOS; 9) 40ppm HAL + 0,5 kg/ton MOS; 10) 40ppm HAL + 1,0kg/ton MOS; 11) 40ppm HAL+ 1,5kg/ton MOS. The results were appraised from 17 to 46 week age, considering the following productive periods (weeks): 1) 17 to 22 week; 2) 23 to 23 week; 3) 27 to 30 week; 4) 31 to 34 week; 5) 35 to 38 week; 6) 39 to 42 week; 7) 43 a 46 week. There were no significant effects (P<0,05) regarding to the results of egg production (%), feed conversion (kg/dz), egg specific gravity, egg weight (g), yolk, albumen and eggshell relative weight (%) and serum total protein and albumin. Feed intake (g/hen/day) was affected by dietary MOS in the 1 Period, with higher intake on the negative control and lower intake attained with the inclusion of 0.700kg/ton MOS. The feed conversion ratio (kg/kg) was affected by dietary treatments on the 4 and 6 Periods and also at the mean result of the Experimental Period, indicating minimum feed conversion ratio with the inclusion of 0.600kg/ton and 0.725kg/ton of MOS on the a Period and on the Experimetal Period, respectively. The inclusion of 19ppm and 28 ppm of HAL was associated to the higher feed conversion ratio obtained for the 6 Period and for the Experimental Period, respectively. The inclusion of 1,5kg/ton of MOS and 40ppm of HAL resulted in higher shell thickness measures for the 2 Period. Hens were slaughtered at 36 weeks of age and the evaluation of the intestinal parameters indicated no significant influence of the additives over the intestine, liver, gizzard and pancreas weight. However, there were indication of reduced measures in crypt depth associated with higher vili:crypth for duodenum and ileum sampleswith the inclusion of 1,5kg/ton MOS and 40ppm HAL. The results indicated that higher levels of MOS and HAL improve egg quality and intestinal parameters, but levels of MOS lower than 1,0kg/ton result in better production for the laying hens.

Tumor Susceptibility Gene 101, TSG101, exhibits multiple biological functions including the regulation of gene transcription, vesicular trafficking, cellular growth and differentiation. However, the signals involve in the regulation of TSG101 gene functions are unclear. In this present study, we observed congruous TSG101 up-regulation and the differentiation status of keratinocyte in both human foreskin tissue and reconstructed organotypic skin culture. In addition, we found an essential and downstream role of TSG101 in calcium-induced early keratinocyte differentiation since TSG101 siRNA inhibits this process. Our results also indicate a PKC-dependent mechanism is involved based on the following findings. First, a PKC agonist, TPA up-regulates TSG101 and keratin 10 under low calcium condition. Second, co-treatment of keratinocytes with GF 109203X, a PKC inhibitor, blocks TPA-induced TSG101 and keratin 10 up-regulation. Previous report indicates TSG101 gene exhibits a TATA-less and Sp1-containing promoter. Our analysis further shows that both calcium and TPA stimulate phosphorylation of Sp1 and the corresponding TSG101 wild type promoter activity, but not the activity of Sp1 site mutant TSG101 promoter. The co-treatment with GF 109203X blocks the above effects of calcium and TPA, implying that this is a PKC signaling-dependent process. Taken together, these data suggest a PKC-Sp1 signaling is involved in early differentiation switch of keratinocyte through up-regulation of TSG101. Functional inactivation experiment indicates that tsg101 is a tumor suppressor in mouse model. However, many studies using human tumor specimens or conditional knockout mouse give discrepant and contradictive results. Therefore, the role of TSG101 in human cancer remains illusive. Here we demonstrate an inverse correlation between TSG101 and p16INK4a or acetylated- histone H4 protein expression profiles in human head and neck squamous cell carcinomas (HNSCC) (N=98, p<0.001). Using conditioned human HEp2 cells, we confirm that TSG101 negatively modulates p16INK4a expression. Chromatin immunoprecipitation and the subsequent PCR analysis reveal that TSG101 dose-dependently decreases the amount of acetylated histone H4-associated chromatin on p16INK4a promoter. In addition, TSG101 interacts and colocalizes with HDAC1 and SUMO-1 in the nucleus. Furthermore, TSG101 confers a dose-dependent effect on promoting HDAC1 SUMOylation, hence its activity. Taken together, our data demonstrate for the first time that TSG101 can promote SUMO-1 modification of HDAC1, which impacts on down-regulation of p16INK4a gene expression, providing evidence whereby TSG101 might participate in the epigenetic silencing of p16INK4a during the development of HNSCC.

Regensburg, Univ., Diss., 2009.

Made available in DSpace on 2018-08-01T22:56:44Z (GMT). No. of bitstreams: 1 tese_11216_Thiago Queiroz de Menezes20171116-154231.pdf: 577045 bytes, checksum: d5c98685b75309cdca78da41f73e2b3d (MD5) Previous issue date: 2017-07-28 / Desempenho e histomorfometria intestinal de frangos de corte alimentados com dietas contendo extrato pirolenhoso. 2017. 63p. Dissertação (Mestrado em Ciências Veterinárias) Centro de Ciências Agrárias e Engenharias, Universidade Federal do Espírito Santo, Alegre, ES, 2017. Nesse estudo se investigou o efeito do extrato pirolenhoso (EPL) sobre o desempenho e histomorfometria intestinal de frangos de corte. Para isso, 1120 frangos de corte machos, da linhagem Cobb 500, de 8 dias de idade, foram distribuídos ao acaso em 8 tratamentos com 7 repetições de 20 aves cada. As rações experimentais formaram os tratamentos, sendo: T1 ração basal (RB); T2 RB contendo 0,4% de EPL; T3 RB contendo 0,8% de EPL; T4 RB contendo 1,2% de EPL; T5 RB contendo 1,6% de EPL; T6 RB contendo enramicina e halquinol; T7 RB contendo enrofloxacina; e T8 RB contendo ácido acético. Foram avaliadas variáveis de desempenho e histomorfometria intestinal nas fases de 08 a 21 e 08 a 35 dias de idade. Não se verificou diferença entre as médias de consumo de ração, ganho de peso e conversão alimentar dos tratamentos contendo EPL (P>0,05). Na fase de 08 a 21 dias de idade, as aves do tratamento contendo 1,2% de EPL apresentaram médias de consumo de ração (P<0,05) e ganho de peso (P<0,05) superiores às médias observadas nas aves dos tratamentos contendo ácido acético. A média de ganho de peso das aves do tratamento contendo 1,2% de EPL ainda foi superior à média de ganho de peso das aves do tratamento contendo enrofloxacina (P<0,05). Na fase de 08 a 35 dias de idade, verificou-se que o ganho de peso das aves do tratamento contendo 1,2% de EPL foi superior (P<0,05) ao ganho de peso apresentado pelas aves dos tratamentos contendo enrofloxacina e ácido acético. Não houve influência dos tratamentos contendo EPL sobre altura de epitélio, altura de vilosidade, largura de vilosidade e profundidade de cripta intestinal das aves (P>0,05). A suplementação com EPL influenciou negativamente a relação altura de vilosidade/profundidade de cripta duodenal (P>0,05). A suplementação com 1,2% de EPL foi capaz de melhorar o desempenho de frangos de corte.

The strain Rhodococcus erythropolis A4 is a source of enzymes nitrilhydratase and amidase, that catalyse conversion of nitriles and amides. These enzymes are used in industrial biotransformation and bioremediation. Since it was difficult to carry out genetic manipulations aimed at increasing the production of these enzymes in the strain A4, the corresponding genes (ami and nha1 + nha2) of a related strain R. erythropolis CCM2595, in which both plasmid and chromosome manipulations can be routinely performed, were identified and analyzed in this diploma theses. The ami and nha1 + nha2 genes from the strain R. erythropolis CCM2595 were isolated and sequenced together with the flanking regions (5.5 kb in total). The organization of these genes and the expected regulatory genes was described in the strain CCM2595 and mechanisms of regulation of expression of these genes were studied. For the analysis of transcription of amidase and nitrilhydratase genes from both strains of R. erythropolis, the promoter-probe vector pEPR1 replicating in Escherichia coli and R. erythropolis was used. Transcriptional fusion of Pami promoters of the strains A4 and CCM2595 and the reporter gfp gene were constructed. The activity of the Pami promoter was measured by means of fluorescence of gfp gene product (green fluorescent...

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